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The map label for this gene is rbsB [H]

Identifier: 150398419

GI number: 150398419

Start: 3402576

End: 3403565

Strand: Direct

Name: rbsB [H]

Synonym: Smed_3227

Alternate gene names: 150398419

Gene position: 3402576-3403565 (Clockwise)

Preceding gene: 150398418

Following gene: 150398420

Centisome position: 89.97

GC content: 61.01

Gene sequence:

>990_bases
ATGAGCATAGCCAAGCGCATTCTTTCCCGCCGGGCCTTCAATGCCCTGGCCGGCGCCGCATTCGTTGCAGCAATGATGCC
TGCGACCTCTTTCGCGCAGGACGTCACGATTCCGATCATCGTCAAGGACACCACGTCCTTCTATTGGCAGATCGTCCTTG
CGGGCGCGCGCGCCGCCGGCAAGGACCTCGGCGTCAATGTGCCGGAACTCGGAGCCCAGTCGGAAGCGGACATCAACGGC
CAGATCACCATCCTCGAAAACGCCGTCGCGAGTGCCCCGGCAGCAGTTGTGATCGCTCCGACGGAGTTCAAGGCACTCGG
CAAGCCTGTCGACGAGGCCGCCAAATCGGTTCCGGTGATCGGCATCGATTCAGGCGCCGATTCCAAGGCGTTCACCTCGT
TCCTGACGACCGACAATGTCCAAGGCGGGCGCATCGCCGCCGACGGCCTCGCGGCTGCAATCAAGGAGGCGACCGGAAAG
GAAGAGGGTGAAATCGCGATCATCACCAGCCTGCCGGGCGTCGGCTCGCTCGATCAGCGCCGCGAAGGTTTTCTCGATCA
GATCAAGACGAAATATCCCGGTCTGAAAGTCGTCGCCGACAAATATGCCGACGGTCAGGCAACGACCGGTCTCAATATCA
TGACCGACCTGATCACAGCCAACCCCAACCTCGTCGGCGTTTTCGCATCCAATCTGATCATGGCGCAGGGCGTCGGCCAG
GCGATCGCCGAGAACAAGCTCGGCGACAAGATCAAGGTGATAGGTTTCGACAGCGACGAAAAGACGGTGGGTTTCCTCAA
GGAGGGCGTGCTGGCCGGCCTCGTCGTCCAGGATCCCTATCGCATGGGCTATGACGGCGTGAAGACGGCCTTGGCAGTCT
CCAAGGGCGAGAAGGTCGAAGCCAATGTCGACACCGGCGCGAACCTGGTCACCAAGGCCAATATGAGCGAGCCGAAGATC
GACGCGCTCTTGAACCCGAAGGTCAACTGA

Upstream 100 bases:

>100_bases
CCTTCGTGGAGAGACGTTCATTTCATAAGCACCGGACCGCATGCACCTGGCGGTCCGTCTCGATAAACGGAACGAGGTGT
GATTTAAGAGGAGAACCCTG

Downstream 100 bases:

>100_bases
CGAAAAGGAAATGCGCGGCGCTGTACGGCAGAGTCGCGCTTTCTTCCGCAATTCAAGAAACTCCGCAACGGCCGGGAGGG
CCGGCCGCTGTGATCCGGAG

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSIAKRILSRRAFNALAGAAFVAAMMPATSFAQDVTIPIIVKDTTSFYWQIVLAGARAAGKDLGVNVPELGAQSEADING
QITILENAVASAPAAVVIAPTEFKALGKPVDEAAKSVPVIGIDSGADSKAFTSFLTTDNVQGGRIAADGLAAAIKEATGK
EEGEIAIITSLPGVGSLDQRREGFLDQIKTKYPGLKVVADKYADGQATTGLNIMTDLITANPNLVGVFASNLIMAQGVGQ
AIAENKLGDKIKVIGFDSDEKTVGFLKEGVLAGLVVQDPYRMGYDGVKTALAVSKGEKVEANVDTGANLVTKANMSEPKI
DALLNPKVN

Sequences:

>Translated_329_residues
MSIAKRILSRRAFNALAGAAFVAAMMPATSFAQDVTIPIIVKDTTSFYWQIVLAGARAAGKDLGVNVPELGAQSEADING
QITILENAVASAPAAVVIAPTEFKALGKPVDEAAKSVPVIGIDSGADSKAFTSFLTTDNVQGGRIAADGLAAAIKEATGK
EEGEIAIITSLPGVGSLDQRREGFLDQIKTKYPGLKVVADKYADGQATTGLNIMTDLITANPNLVGVFASNLIMAQGVGQ
AIAENKLGDKIKVIGFDSDEKTVGFLKEGVLAGLVVQDPYRMGYDGVKTALAVSKGEKVEANVDTGANLVTKANMSEPKI
DALLNPKVN
>Mature_328_residues
SIAKRILSRRAFNALAGAAFVAAMMPATSFAQDVTIPIIVKDTTSFYWQIVLAGARAAGKDLGVNVPELGAQSEADINGQ
ITILENAVASAPAAVVIAPTEFKALGKPVDEAAKSVPVIGIDSGADSKAFTSFLTTDNVQGGRIAADGLAAAIKEATGKE
EGEIAIITSLPGVGSLDQRREGFLDQIKTKYPGLKVVADKYADGQATTGLNIMTDLITANPNLVGVFASNLIMAQGVGQA
IAENKLGDKIKVIGFDSDEKTVGFLKEGVLAGLVVQDPYRMGYDGVKTALAVSKGEKVEANVDTGANLVTKANMSEPKID
ALLNPKVN

Specific function: Involved in the high-affinity D-ribose membrane transport system and also serves as the primary chemoreceptor for chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=309, Percent_Identity=30.42071197411, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790526, Length=287, Percent_Identity=28.5714285714286, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 34105; Mature: 33974

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAKRILSRRAFNALAGAAFVAAMMPATSFAQDVTIPIIVKDTTSFYWQIVLAGARAAG
CCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEEEECCCHHEEEEEECCCCCCC
KDLGVNVPELGAQSEADINGQITILENAVASAPAAVVIAPTEFKALGKPVDEAAKSVPVI
CCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCEEEEEECHHHHHHCCCHHHHHHCCCEE
GIDSGADSKAFTSFLTTDNVQGGRIAADGLAAAIKEATGKEEGEIAIITSLPGVGSLDQR
EECCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHH
REGFLDQIKTKYPGLKVVADKYADGQATTGLNIMTDLITANPNLVGVFASNLIMAQGVGQ
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
AIAENKLGDKIKVIGFDSDEKTVGFLKEGVLAGLVVQDPYRMGYDGVKTALAVSKGEKVE
HHHHHCCCCEEEEEEECCCCCHHHHHHHHHHEEEEEECCHHCCCHHHHHHHHHCCCCEEE
ANVDTGANLVTKANMSEPKIDALLNPKVN
ECCCCCCCEEEECCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SIAKRILSRRAFNALAGAAFVAAMMPATSFAQDVTIPIIVKDTTSFYWQIVLAGARAAG
CHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCEEEEEEECCCHHEEEEEECCCCCCC
KDLGVNVPELGAQSEADINGQITILENAVASAPAAVVIAPTEFKALGKPVDEAAKSVPVI
CCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCEEEEEECHHHHHHCCCHHHHHHCCCEE
GIDSGADSKAFTSFLTTDNVQGGRIAADGLAAAIKEATGKEEGEIAIITSLPGVGSLDQR
EECCCCCHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHH
REGFLDQIKTKYPGLKVVADKYADGQATTGLNIMTDLITANPNLVGVFASNLIMAQGVGQ
HHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEHHHHHHHHHHHHH
AIAENKLGDKIKVIGFDSDEKTVGFLKEGVLAGLVVQDPYRMGYDGVKTALAVSKGEKVE
HHHHHCCCCEEEEEEECCCCCHHHHHHHHHHEEEEEECCHHCCCHHHHHHHHHCCCCEEE
ANVDTGANLVTKANMSEPKIDALLNPKVN
ECCCCCCCEEEECCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6313683; 7686882; 9278503; 3011793; 3011794; 8899705; 9298646; 1583688; 7982928; 9641984 [H]