Click here to switch to the map view.

The map label for this gene is garD [H]

Identifier: 150398417

GI number: 150398417

Start: 3399877

End: 3401382

Strand: Direct

Name: garD [H]

Synonym: Smed_3225

Alternate gene names: 150398417

Gene position: 3399877-3401382 (Clockwise)

Preceding gene: 150398415

Following gene: 150398418

Centisome position: 89.9

GC content: 64.67

Gene sequence:

>1506_bases
TTGCCCGCACCGTCATCGATTCTTCTTTCAACTGACGACAACGTCGTCGTCGCCACCGCGGCGATCGCGCCGGGTGACAG
GCTGGTCGGCGAAGTATCCGCAGTCGCGCGGATAGAGCCGGGCCACAAGGCCGCGGTCCGCGCGATCGAGGCAGGCGAGG
CCGTGGTAAAATATGGTCAGGCAATCGGCCGTGCCACCTCGCCCATTGCCCCTGGCGAACACGTCCATTCGCATAACCTC
GCCTTCGATCAGGGTCGGCTGGCCATTGGCGCTCCCGTTCCGCCGGAGGCTGCGAGCGAGGCCGACAGGACGCGCACATT
CCTCGGCTACCGCCGGGCGGACGGGCGTGCGGCTACGCGCAACTATATCGGCATCGTCGCCAGCGTGAACTGTTCCACCA
CGGTCTGTCGCGCAATCGCCGATGAGGCGAACCGGCGCGTCCTGCCCAAGTATGAAGGCATCGACGGCTTCGTTCCGATC
GTCCATGACCAGGGATGCGGCATGTCGTCCACCGGCGACGGAATGAAGAACCTGCACAGGACGCTCGCCGGCTATGCGCG
CCACGCGAATTTCGGCGGCGTGCTGATGGTGGGGCTCGGTTGCGAGGTCAATCAGCTGACGCTCTACGGCCAGAGCGGAG
CGGGGGCCGAGAAACGGCATTTCAACATTCAGGAGGCTGGCGGTTCGCGCCGCTCGGTGGAACGCGCCCTCGGCATTCTC
GAGGAGATCGCGAAGGAGGTAGCGACAGCCCGGCGTGTGCCGATCCCGGTCAGCGAAATCATCGTCGGCCTGCAATGCGG
TGGTTCGGACGGTCTGTCGGGCATCACCGCCAACCCGGCGCTCGGTGCGGCGGTCGATATACTTGCCGGCGCCGGCGGCA
CCGCCATCCTCTCCGAAACCTCCGAGATCTACGGGGCCGAGCATCTGCTGCGCAGCCGCGCGGTCAACGAGGCGGTCGCG
GTCAAGCTGGACGGGCTGATCGCTTGGTGGGAAGATTATGTGGCGATGCATGGCGCATCGCTCGACAATAATCCTTCGCC
GGGTAACAAGAGGGGCGGTTTGACGACGATCCTCGAAAAATCGCTGGGTGCCGTCGCCAAGGGCGGCCGATCGCCTTTGA
CGGCCGTTTATAATTACGCGGAACGCGTGACGGAGCCCGGCCTGGTTTTCATGGACACGCCCGGCTACGATCCGGTCTCG
GCAACCGGCCAGGTCGCCGGCGGCGCCAACGTCATCGCGTTCACGACCGGACGCGGCAGCTGTTTCGGGTGTCGGCCGGC
GCCATCGATCAAGCTCACCAGCAACACGGCGCTTTATCAGGCGATGGAAGAGGACATGGACATTGACTGCGGCATCATCG
CATCGGGAGAAGCCACGATTGCAGGCATGGGCCGGGGGATCTTCGATCTGATCATAGAGACCGCCTCGGGCCGCAAGACC
AAGAGCGAGCTTTTCGGCTACGGCGACAACGAGTTCGTGCCATGGCACCTGGGGGCAACGCTTTAG

Upstream 100 bases:

>100_bases
GCCAGAGCCATAATAGCCATTGACGAACTTCTGTCTATTGTAGATAAAAGACAGAAGTGATGCCCGCCCAGATTGGGGAA
ACGTTCGGGAGGAGTTTGAT

Downstream 100 bases:

>100_bases
GCGGGGAGATGCGGGGCAGCACCAGGCGTATTAAGGGCGCAATCCTTGGAGGAGGAGGCAAGGGATGCAGACGAGAAGGA
TCGGACGGACCGCGCTTGCG

Product: Altronate dehydratase

Products: NA

Alternate protein names: GalcD [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MPAPSSILLSTDDNVVVATAAIAPGDRLVGEVSAVARIEPGHKAAVRAIEAGEAVVKYGQAIGRATSPIAPGEHVHSHNL
AFDQGRLAIGAPVPPEAASEADRTRTFLGYRRADGRAATRNYIGIVASVNCSTTVCRAIADEANRRVLPKYEGIDGFVPI
VHDQGCGMSSTGDGMKNLHRTLAGYARHANFGGVLMVGLGCEVNQLTLYGQSGAGAEKRHFNIQEAGGSRRSVERALGIL
EEIAKEVATARRVPIPVSEIIVGLQCGGSDGLSGITANPALGAAVDILAGAGGTAILSETSEIYGAEHLLRSRAVNEAVA
VKLDGLIAWWEDYVAMHGASLDNNPSPGNKRGGLTTILEKSLGAVAKGGRSPLTAVYNYAERVTEPGLVFMDTPGYDPVS
ATGQVAGGANVIAFTTGRGSCFGCRPAPSIKLTSNTALYQAMEEDMDIDCGIIASGEATIAGMGRGIFDLIIETASGRKT
KSELFGYGDNEFVPWHLGATL

Sequences:

>Translated_501_residues
MPAPSSILLSTDDNVVVATAAIAPGDRLVGEVSAVARIEPGHKAAVRAIEAGEAVVKYGQAIGRATSPIAPGEHVHSHNL
AFDQGRLAIGAPVPPEAASEADRTRTFLGYRRADGRAATRNYIGIVASVNCSTTVCRAIADEANRRVLPKYEGIDGFVPI
VHDQGCGMSSTGDGMKNLHRTLAGYARHANFGGVLMVGLGCEVNQLTLYGQSGAGAEKRHFNIQEAGGSRRSVERALGIL
EEIAKEVATARRVPIPVSEIIVGLQCGGSDGLSGITANPALGAAVDILAGAGGTAILSETSEIYGAEHLLRSRAVNEAVA
VKLDGLIAWWEDYVAMHGASLDNNPSPGNKRGGLTTILEKSLGAVAKGGRSPLTAVYNYAERVTEPGLVFMDTPGYDPVS
ATGQVAGGANVIAFTTGRGSCFGCRPAPSIKLTSNTALYQAMEEDMDIDCGIIASGEATIAGMGRGIFDLIIETASGRKT
KSELFGYGDNEFVPWHLGATL
>Mature_500_residues
PAPSSILLSTDDNVVVATAAIAPGDRLVGEVSAVARIEPGHKAAVRAIEAGEAVVKYGQAIGRATSPIAPGEHVHSHNLA
FDQGRLAIGAPVPPEAASEADRTRTFLGYRRADGRAATRNYIGIVASVNCSTTVCRAIADEANRRVLPKYEGIDGFVPIV
HDQGCGMSSTGDGMKNLHRTLAGYARHANFGGVLMVGLGCEVNQLTLYGQSGAGAEKRHFNIQEAGGSRRSVERALGILE
EIAKEVATARRVPIPVSEIIVGLQCGGSDGLSGITANPALGAAVDILAGAGGTAILSETSEIYGAEHLLRSRAVNEAVAV
KLDGLIAWWEDYVAMHGASLDNNPSPGNKRGGLTTILEKSLGAVAKGGRSPLTAVYNYAERVTEPGLVFMDTPGYDPVSA
TGQVAGGANVIAFTTGRGSCFGCRPAPSIKLTSNTALYQAMEEDMDIDCGIIASGEATIAGMGRGIFDLIIETASGRKTK
SELFGYGDNEFVPWHLGATL

Specific function: D-galactarate metabolism; first step. [C]

COG id: COG2721

COG function: function code G; Altronate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uxaA family [H]

Homologues:

Organism=Escherichia coli, GI1789516, Length=505, Percent_Identity=36.039603960396, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1789475, Length=481, Percent_Identity=35.966735966736, Blast_Score=266, Evalue=2e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007392
- InterPro:   IPR017654
- InterPro:   IPR013974 [H]

Pfam domain/function: PF04295 GD_AH_C; PF08666 SAF [H]

EC number: =4.2.1.42 [H]

Molecular weight: Translated: 52006; Mature: 51875

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAPSSILLSTDDNVVVATAAIAPGDRLVGEVSAVARIEPGHKAAVRAIEAGEAVVKYGQ
CCCCCCEEEECCCCEEEEEEEECCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHH
AIGRATSPIAPGEHVHSHNLAFDQGRLAIGAPVPPEAASEADRTRTFLGYRRADGRAATR
HHCCCCCCCCCCCCCCCCCCEECCCCEEECCCCCCHHHHHHHHHHHHHEEECCCCCHHCC
NYIGIVASVNCSTTVCRAIADEANRRVLPKYEGIDGFVPIVHDQGCGMSSTGDGMKNLHR
CEEEEEEECCCHHHHHHHHHHHHCCEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHH
TLAGYARHANFGGVLMVGLGCEVNQLTLYGQSGAGAEKRHFNIQEAGGSRRSVERALGIL
HHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCCCHHCCCHHHCCCCHHHHHHHHHHH
EEIAKEVATARRVPIPVSEIIVGLQCGGSDGLSGITANPALGAAVDILAGAGGTAILSET
HHHHHHHHHHCCCCCCHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEHHCH
SEIYGAEHLLRSRAVNEAVAVKLDGLIAWWEDYVAMHGASLDNNPSPGNKRGGLTTILEK
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
SLGAVAKGGRSPLTAVYNYAERVTEPGLVFMDTPGYDPVSATGQVAGGANVIAFTTGRGS
HHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCC
CFGCRPAPSIKLTSNTALYQAMEEDMDIDCGIIASGEATIAGMGRGIFDLIIETASGRKT
EEECCCCCCEEECCCHHHHHHHHHCCCCCCEEEECCCEEEEECCCCHHHHHHCCCCCCCC
KSELFGYGDNEFVPWHLGATL
HHHHCCCCCCCEEEEECCCCC
>Mature Secondary Structure 
PAPSSILLSTDDNVVVATAAIAPGDRLVGEVSAVARIEPGHKAAVRAIEAGEAVVKYGQ
CCCCCEEEECCCCEEEEEEEECCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHHHH
AIGRATSPIAPGEHVHSHNLAFDQGRLAIGAPVPPEAASEADRTRTFLGYRRADGRAATR
HHCCCCCCCCCCCCCCCCCCEECCCCEEECCCCCCHHHHHHHHHHHHHEEECCCCCHHCC
NYIGIVASVNCSTTVCRAIADEANRRVLPKYEGIDGFVPIVHDQGCGMSSTGDGMKNLHR
CEEEEEEECCCHHHHHHHHHHHHCCEECCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHH
TLAGYARHANFGGVLMVGLGCEVNQLTLYGQSGAGAEKRHFNIQEAGGSRRSVERALGIL
HHHHHHHCCCCCCEEEEECCCCEEEEEEEECCCCCCCHHCCCHHHCCCCHHHHHHHHHHH
EEIAKEVATARRVPIPVSEIIVGLQCGGSDGLSGITANPALGAAVDILAGAGGTAILSET
HHHHHHHHHHCCCCCCHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCEEHHCH
SEIYGAEHLLRSRAVNEAVAVKLDGLIAWWEDYVAMHGASLDNNPSPGNKRGGLTTILEK
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHH
SLGAVAKGGRSPLTAVYNYAERVTEPGLVFMDTPGYDPVSATGQVAGGANVIAFTTGRGS
HHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCC
CFGCRPAPSIKLTSNTALYQAMEEDMDIDCGIIASGEATIAGMGRGIFDLIIETASGRKT
EEECCCCCCEEECCCHHHHHHHHHCCCCCCEEEECCCEEEEECCCCHHHHHHCCCCCCCC
KSELFGYGDNEFVPWHLGATL
HHHHCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704254; 9384377 [H]