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The map label for this gene is nuoB1 [H]

Identifier: 150396112

GI number: 150396112

Start: 960748

End: 961326

Strand: Direct

Name: nuoB1 [H]

Synonym: Smed_0889

Alternate gene names: 150396112

Gene position: 960748-961326 (Clockwise)

Preceding gene: 150396111

Following gene: 150396113

Centisome position: 25.4

GC content: 62.52

Gene sequence:

>579_bases
ATGGAATTAGCATCCGGCACCACGCTCGTTGCGCCGCAGCCCAAGGGCATCCTGGATCCGGCCACCGGCAAGCCGATCGG
CAGCAATGACGCATTCTTCGGCGAGATCAACAACGAGCTCGCCGACAAGGGCTTTCTCGTCACCTCGACCGACGAGCTTA
TAACCTGGGCCCGCACCGGCTCGCTGATGTGGATGACCTTCGGGCTTGCTTGCTGTGCGGTCGAGATGATGCAGATGTCG
ATGCCGCGTTACGACGCCGAGCGTTTCGGTTTCGCGCCGCGCGCCTCACCGCGCCAGTCCGACGTCATGATCGTCGCCGG
TACGCTCACCAACAAGATGGCGCCCGCGCTTCGCAAGGTCTACGACCAGATGCCGGAGCCGCGTTACGTGATCTCGATGG
GATCCTGCGCCAATGGCGGCGGCTATTATCACTATTCCTATTCGGTTGTGCGCGGCTGCGATCGCGTCGTGCCCGTCGAC
ATCTACGTGCCAGGCTGTCCTCCCACGGCAGAAGCGCTGCTTTACGGCGTGCTTCTGCTGCAGAAGAAGATCCGCCGGAC
CGGCACGATCGAGCGCTAA

Upstream 100 bases:

>100_bases
CGTTCAAGGCGATCGGCTGGTTCGGCTTCTGGTCGATGATGGTCTTCCTTCTGGTCCTGACGGTCGGCTTTATCTATGAA
TGGAAGAAGGGAGCGCTGGA

Downstream 100 bases:

>100_bases
GGGCAGGGGAACTTTGACATGAGTGAAGCCCTGAACGAGCTTGCCTCCTATATTCGCGAGGCGCGAGGCGCGCTGGTCGC
CGGATCGGAAGTCAGATACG

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B 1; NDH-1 subunit B 1 [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MELASGTTLVAPQPKGILDPATGKPIGSNDAFFGEINNELADKGFLVTSTDELITWARTGSLMWMTFGLACCAVEMMQMS
MPRYDAERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVD
IYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Sequences:

>Translated_192_residues
MELASGTTLVAPQPKGILDPATGKPIGSNDAFFGEINNELADKGFLVTSTDELITWARTGSLMWMTFGLACCAVEMMQMS
MPRYDAERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVD
IYVPGCPPTAEALLYGVLLLQKKIRRTGTIER
>Mature_192_residues
MELASGTTLVAPQPKGILDPATGKPIGSNDAFFGEINNELADKGFLVTSTDELITWARTGSLMWMTFGLACCAVEMMQMS
MPRYDAERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVD
IYVPGCPPTAEALLYGVLLLQKKIRRTGTIER

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=78.2312925170068, Blast_Score=251, Evalue=3e-67,
Organism=Escherichia coli, GI1788624, Length=168, Percent_Identity=44.6428571428571, Blast_Score=150, Evalue=4e-38,
Organism=Escherichia coli, GI1789074, Length=126, Percent_Identity=43.6507936507937, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788834, Length=123, Percent_Identity=41.4634146341463, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17509685, Length=149, Percent_Identity=67.7852348993289, Blast_Score=224, Evalue=2e-59,
Organism=Drosophila melanogaster, GI18859983, Length=159, Percent_Identity=70.440251572327, Blast_Score=246, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24642371, Length=159, Percent_Identity=70.440251572327, Blast_Score=246, Evalue=8e-66,
Organism=Drosophila melanogaster, GI24651058, Length=166, Percent_Identity=67.4698795180723, Blast_Score=240, Evalue=4e-64,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20990; Mature: 20990

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
8.3 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
8.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELASGTTLVAPQPKGILDPATGKPIGSNDAFFGEINNELADKGFLVTSTDELITWARTG
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEHHCHHHCCCCEEEECHHHHEEEHHCC
SLMWMTFGLACCAVEMMQMSMPRYDAERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
YDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGVLLL
HHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHH
QKKIRRTGTIER
HHHHHHCCCCCC
>Mature Secondary Structure
MELASGTTLVAPQPKGILDPATGKPIGSNDAFFGEINNELADKGFLVTSTDELITWARTG
CCCCCCCEEECCCCCCCCCCCCCCCCCCCCCEEEHHCHHHCCCCEEEECHHHHEEEHHCC
SLMWMTFGLACCAVEMMQMSMPRYDAERFGFAPRASPRQSDVMIVAGTLTNKMAPALRKV
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCCCCEEEEEECHHHHHHHHHHHH
YDQMPEPRYVISMGSCANGGGYYHYSYSVVRGCDRVVPVDIYVPGCPPTAEALLYGVLLL
HHHCCCCEEEEEECCCCCCCCEEEEEEHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHH
QKKIRRTGTIER
HHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA