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The map label for this gene is aspS [H]

Identifier: 150396026

GI number: 150396026

Start: 863504

End: 865291

Strand: Direct

Name: aspS [H]

Synonym: Smed_0802

Alternate gene names: 150396026

Gene position: 863504-865291 (Clockwise)

Preceding gene: 150396020

Following gene: 150396027

Centisome position: 22.83

GC content: 62.19

Gene sequence:

>1788_bases
ATGCACCGTTACCGCAGCCACACCTGTGCCGCCCTCCGCAAGTCGGATGTCGGCTCCACCGTTCGCCTTTCCGGCTGGGT
TCACCGCGTCCGCGATCACGGCGGCGTGCTCTTCATCGATTTGCGCGACCATTATGGCATGACCCAGGTCGTCGCCGATC
CGGATTCGCCGGCCTTCAAAGCGGCCGAGACGGTGCGCGGCGAATGGGTCATTCGCGTCGACGGTGCCGTCAAGGCGCGC
ACCGACGAGACGGTCAACCGGAACATGCCTACGGGTGAGGTCGAGCTTTATGCCCGCGAGATCGAGGTGCTCTCGGCCGC
CAAGGAACTGCCGCTGCCGGTTTTCGGCGAGCCGGACTATCCCGAAGACGTGCGCCTGAAATACCGCTTCCTCGATCTGC
GCCGCGAGACGCTGCACAAGAACATCGTCAGGCGCACCGAAATCATCGCCGCCATGCGGCGCCGGATGAGCGACATAGGC
TTCACCGAATATACGACCCCGATCCTGACGGCCTCGTCGCCGGAAGGCGCGCGCGACTTCCTGGTGCCCAGCCGCATCCA
CCCCGGAAATTTCTATGCGCTGCCGCAGGCCCCTCAGCAGTACAAGCAGCTCCTGATGGTTGCGGGCTTTGACCGGTACT
TCCAGATTGCTCCGTGTTTCCGCGACGAGGATCCGCGCGCCGACCGTCTGCCCGGCGAGTTCTATCAGCTCGATCTCGAA
ATGAGCTTCGTCGAACAGGAGGACGTTTGGGACGCCATGGAGCCGATGATCCGGGCGATCTTCGCGGATTTCGCCGGAGG
CAAGCCGGTGACGGACAAGTTCCCGCGCATTCCCTATGACGCGGCGATCCGCAAATATGGTTCCGATAAGCCGGACCTGC
GCAACCCGATCGAGATGCAGGAGGTTACGGAGCATTTCGCCGGTTCCGGCTTCAAGGTCTTCGCCAATATGATAGCCTCC
AACCCGAAGGTGGAGATCTGGGCCATTCCCGCCAAGACCGGCGGCTCTCGCGCTTTCTGCGACCGTATGAACGCCTGGGC
TCAAAGCCAGGGTCAGCCGGGCCTCGGATATATATTCTGGCGCAAGGAAGGCGAGAAGCTCGAAGGTGCCGGTCCGCTCG
CCAAGAATATCGGCGAGGAGCGGACCGACGCCATCCGCACGCAACTCGGTCTCGGGGACGGCGACGCCTGCTTCTTCGTT
GCCGGGGAACCGGCGAAATTCTACAAGTTCGCCGGCGAAGCCCGCACAAGGGCAGGCGAGGAATTGAACCTCGTCGACCG
TGAGCGTTACGAGCTGTGCTGGATCGTGGACTTCCCGTTCTATGAATGGAACGAGGAGGAAAAGCGCGTCGATTTCGCCC
ACAACCCGTTCTCGATGCCGCAGGGTGGCCTCACTGCTCTTTCGAGCGACGATCTCCTGTCGATCAAGGCTTTCCAGTAC
GACATGGTGTGCAACGGCTTCGAGATCGCCTCGGGTTCGATCCGTAACCAGTCGCCGGAACTCATGGTGAAGGCTTTCGA
GAATGTCGGGCTCAGCCAGGCTGACGTGGAAGAGCGGTTCGGTGGTCTCTACCGCGCGTTCCAATATGGTGCCCCGCCGC
ATGGCGGCATGGCTTTCGGCATCGATCGCATCGTCATGCTGCTGGTCGGCGCCAAGAATTTGCGCGAAATCTCTCTGTTT
CCGATGAACCAGCAGGCTCAGGACCTGCTGATGGGGGCCCCTTCGCCGGCGGAGCCGGCGCAGCTCAGGGAACTGGCGAT
CCGACCGGTACCGCAGAAGAAGGATTGA

Upstream 100 bases:

>100_bases
AAACCGGCGCATCATGCGCTTTTCCGCCCGACTTTGATCATGCCGCCCTGCGCCGTTTGCGACACGCGCGGTCTTAAGCA
ATTCCGGGACTATATATACG

Downstream 100 bases:

>100_bases
TCATCCTTGCAGGGAAAACCCGGCGATGAACGCGCCGGATTTTCGCTCGCTTCTAGCCTCACCTCTATGCTTGTCACAGG
AATCCTGTGTGTCGAAACTG

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 595; Mature: 595

Protein sequence:

>595_residues
MHRYRSHTCAALRKSDVGSTVRLSGWVHRVRDHGGVLFIDLRDHYGMTQVVADPDSPAFKAAETVRGEWVIRVDGAVKAR
TDETVNRNMPTGEVELYAREIEVLSAAKELPLPVFGEPDYPEDVRLKYRFLDLRRETLHKNIVRRTEIIAAMRRRMSDIG
FTEYTTPILTASSPEGARDFLVPSRIHPGNFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVEQEDVWDAMEPMIRAIFADFAGGKPVTDKFPRIPYDAAIRKYGSDKPDLRNPIEMQEVTEHFAGSGFKVFANMIAS
NPKVEIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWRKEGEKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFV
AGEPAKFYKFAGEARTRAGEELNLVDRERYELCWIVDFPFYEWNEEEKRVDFAHNPFSMPQGGLTALSSDDLLSIKAFQY
DMVCNGFEIASGSIRNQSPELMVKAFENVGLSQADVEERFGGLYRAFQYGAPPHGGMAFGIDRIVMLLVGAKNLREISLF
PMNQQAQDLLMGAPSPAEPAQLRELAIRPVPQKKD

Sequences:

>Translated_595_residues
MHRYRSHTCAALRKSDVGSTVRLSGWVHRVRDHGGVLFIDLRDHYGMTQVVADPDSPAFKAAETVRGEWVIRVDGAVKAR
TDETVNRNMPTGEVELYAREIEVLSAAKELPLPVFGEPDYPEDVRLKYRFLDLRRETLHKNIVRRTEIIAAMRRRMSDIG
FTEYTTPILTASSPEGARDFLVPSRIHPGNFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVEQEDVWDAMEPMIRAIFADFAGGKPVTDKFPRIPYDAAIRKYGSDKPDLRNPIEMQEVTEHFAGSGFKVFANMIAS
NPKVEIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWRKEGEKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFV
AGEPAKFYKFAGEARTRAGEELNLVDRERYELCWIVDFPFYEWNEEEKRVDFAHNPFSMPQGGLTALSSDDLLSIKAFQY
DMVCNGFEIASGSIRNQSPELMVKAFENVGLSQADVEERFGGLYRAFQYGAPPHGGMAFGIDRIVMLLVGAKNLREISLF
PMNQQAQDLLMGAPSPAEPAQLRELAIRPVPQKKD
>Mature_595_residues
MHRYRSHTCAALRKSDVGSTVRLSGWVHRVRDHGGVLFIDLRDHYGMTQVVADPDSPAFKAAETVRGEWVIRVDGAVKAR
TDETVNRNMPTGEVELYAREIEVLSAAKELPLPVFGEPDYPEDVRLKYRFLDLRRETLHKNIVRRTEIIAAMRRRMSDIG
FTEYTTPILTASSPEGARDFLVPSRIHPGNFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
MSFVEQEDVWDAMEPMIRAIFADFAGGKPVTDKFPRIPYDAAIRKYGSDKPDLRNPIEMQEVTEHFAGSGFKVFANMIAS
NPKVEIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFWRKEGEKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFV
AGEPAKFYKFAGEARTRAGEELNLVDRERYELCWIVDFPFYEWNEEEKRVDFAHNPFSMPQGGLTALSSDDLLSIKAFQY
DMVCNGFEIASGSIRNQSPELMVKAFENVGLSQADVEERFGGLYRAFQYGAPPHGGMAFGIDRIVMLLVGAKNLREISLF
PMNQQAQDLLMGAPSPAEPAQLRELAIRPVPQKKD

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=598, Percent_Identity=33.7792642140468, Blast_Score=318, Evalue=9e-87,
Organism=Homo sapiens, GI45439306, Length=293, Percent_Identity=29.0102389078498, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI226491502, Length=255, Percent_Identity=27.843137254902, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI194272210, Length=260, Percent_Identity=27.6923076923077, Blast_Score=78, Evalue=3e-14,
Organism=Homo sapiens, GI5031815, Length=260, Percent_Identity=27.6923076923077, Blast_Score=78, Evalue=3e-14,
Organism=Escherichia coli, GI1788173, Length=595, Percent_Identity=40.672268907563, Blast_Score=443, Evalue=1e-125,
Organism=Escherichia coli, GI1789256, Length=291, Percent_Identity=28.5223367697594, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790571, Length=220, Percent_Identity=30.9090909090909, Blast_Score=79, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI32566633, Length=607, Percent_Identity=32.7841845140033, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17551876, Length=254, Percent_Identity=30.3149606299213, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71984122, Length=296, Percent_Identity=26.6891891891892, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71994340, Length=300, Percent_Identity=26.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535925, Length=300, Percent_Identity=26.3333333333333, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17535927, Length=300, Percent_Identity=26.3333333333333, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=660, Percent_Identity=27.8787878787879, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=271, Percent_Identity=28.4132841328413, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=255, Percent_Identity=25.8823529411765, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584738, Length=599, Percent_Identity=33.7228714524207, Blast_Score=303, Evalue=3e-82,
Organism=Drosophila melanogaster, GI17136276, Length=245, Percent_Identity=29.3877551020408, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24640849, Length=273, Percent_Identity=26.3736263736264, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24640851, Length=273, Percent_Identity=26.3736263736264, Blast_Score=74, Evalue=3e-13,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 67095; Mature: 67095

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRYRSHTCAALRKSDVGSTVRLSGWVHRVRDHGGVLFIDLRDHYGMTQVVADPDSPAFK
CCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHH
AAETVRGEWVIRVDGAVKARTDETVNRNMPTGEVELYAREIEVLSAAKELPLPVFGEPDY
HHHHCCCCEEEEECCCEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDVRLKYRFLDLRRETLHKNIVRRTEIIAAMRRRMSDIGFTEYTTPILTASSPEGARDF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHC
LVPSRIHPGNFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVEQEDVWDAMEPMIRAIFADFAGGKPVTDKFPRIPYDAAIRKYGSDKPDLRNPIEMQ
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHH
EVTEHFAGSGFKVFANMIASNPKVEIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFW
HHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
RKEGEKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFVAGEPAKFYKFAGEARTRAGE
CCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCHHHHCCCC
ELNLVDRERYELCWIVDFPFYEWNEEEKRVDFAHNPFSMPQGGLTALSSDDLLSIKAFQY
CCCCCCCCCEEEEEEEECCCCCCCCHHHCEEECCCCCCCCCCCCEEECCCCEEEEEEEEE
DMVCNGFEIASGSIRNQSPELMVKAFENVGLSQADVEERFGGLYRAFQYGAPPHGGMAFG
EEEECCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IDRIVMLLVGAKNLREISLFPMNQQAQDLLMGAPSPAEPAQLRELAIRPVPQKKD
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MHRYRSHTCAALRKSDVGSTVRLSGWVHRVRDHGGVLFIDLRDHYGMTQVVADPDSPAFK
CCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCCEEEEEECCCCCCEEEEECCCCCHHH
AAETVRGEWVIRVDGAVKARTDETVNRNMPTGEVELYAREIEVLSAAKELPLPVFGEPDY
HHHHCCCCEEEEECCCEEECCCCHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PEDVRLKYRFLDLRRETLHKNIVRRTEIIAAMRRRMSDIGFTEYTTPILTASSPEGARDF
CCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCHHC
LVPSRIHPGNFYALPQAPQQYKQLLMVAGFDRYFQIAPCFRDEDPRADRLPGEFYQLDLE
CCCCCCCCCCEEECCCCHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEEEEEEE
MSFVEQEDVWDAMEPMIRAIFADFAGGKPVTDKFPRIPYDAAIRKYGSDKPDLRNPIEMQ
EEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCHH
EVTEHFAGSGFKVFANMIASNPKVEIWAIPAKTGGSRAFCDRMNAWAQSQGQPGLGYIFW
HHHHHHCCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEE
RKEGEKLEGAGPLAKNIGEERTDAIRTQLGLGDGDACFFVAGEPAKFYKFAGEARTRAGE
CCCCCCCCCCCCHHHHCCHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHCHHHHCCCC
ELNLVDRERYELCWIVDFPFYEWNEEEKRVDFAHNPFSMPQGGLTALSSDDLLSIKAFQY
CCCCCCCCCEEEEEEEECCCCCCCCHHHCEEECCCCCCCCCCCCEEECCCCEEEEEEEEE
DMVCNGFEIASGSIRNQSPELMVKAFENVGLSQADVEERFGGLYRAFQYGAPPHGGMAFG
EEEECCEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHH
IDRIVMLLVGAKNLREISLFPMNQQAQDLLMGAPSPAEPAQLRELAIRPVPQKKD
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]