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The map label for this gene is gcvA [H]

Identifier: 150395977

GI number: 150395977

Start: 809248

End: 810156

Strand: Direct

Name: gcvA [H]

Synonym: Smed_0753

Alternate gene names: 150395977

Gene position: 809248-810156 (Clockwise)

Preceding gene: 150395972

Following gene: 150395978

Centisome position: 21.4

GC content: 62.27

Gene sequence:

>909_bases
GTGCGGATACCCTCGACACAGGCACTTCGTGCACTCGAGAGCTTTGCGCGACACGGCACCGTCTGGCGCGCAGCGGAAGA
ACTGCACCTGACCCGAAGCGCGGTGAGCCACCAATTGCGGCTTCTCGAGCGTGAGCTGGGTTTTGACCTCCTCGAGCGGG
TCGGCAAAGGCGTAGCATTGACCGCGCGCGGCCGGCGTTATGCCCATGATGTGCACAAGGCGCTGACGATGATCGGTGAC
GCCGGGGCGCAGAACCGCGGCGGTGGTATCGGCGGCTCGTTCCGCGTAAGCTGCACGCCCGGCTTTGCCTCTCTCTGGCT
GTGCACCCATATCGCGGAGTTCCAGGAGATGTATCAGGACGTCTCCTTGTCCATCGTGACGCCGCGACGTCTCGACGACG
TGAGCAATCCGGACGTCGATGCGTTCATCGCCTTTGGTGACGGAAACTGGCCCAATTGCGGCGTCGAGCTGTTGTGCGAC
GTCCTCTTCACTCCCCTCTGCAGTCCGAGCCTTCTCAATAGGCTGGGAGGGTTTTCCAAGCCGGCCGATGTGTTGCGCGC
AAATCTGCTTCACCTCAACGATGTCGACGACTGGTCGCGCTGGCTTTCGCTCACTAAGGTGGAAACGGCGGACCCATCCG
GCGGCATCTTCTTTTCGGACATGAACCTGGTTTTCTCCGCAGCCATTGCCGGGCAGGGAATAGCCATGGGCGATTCGCTC
ACCGGGCAGACGGCATTGAGCGAGGGCAGACTCGTACGCCCGTTCGACATCGCGGTTCCCGCAGCCCGCTCCTATTACCT
CGTTGTGGACCTGGCCAAGTCCGACCATGCGGTCCTCTTTGCTTTCCGGGATTGGCTTCGGTCGAGCCTCGCGGATGCAG
CGGCCGATATGCAAGCCGCTCGAGGATAG

Upstream 100 bases:

>100_bases
GGGTTGCACCTGCTCGCAAAGAAAGCAGTGAAATGCTTGCACAAAAAGCGATATGTTTTATCGTTTCGTGCAAATTTCGT
TCACCACAGGAGGTTCGATC

Downstream 100 bases:

>100_bases
CCGGTCGAAAGGTGAATCAAATTTGCCAATAAGGCGAAAATTATTCGGTTGCCGTGAGCCGATTGCGCGCATAGGCTTTC
CCAGCAGGTGAGGGAGAGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRIPSTQALRALESFARHGTVWRAAEELHLTRSAVSHQLRLLERELGFDLLERVGKGVALTARGRRYAHDVHKALTMIGD
AGAQNRGGGIGGSFRVSCTPGFASLWLCTHIAEFQEMYQDVSLSIVTPRRLDDVSNPDVDAFIAFGDGNWPNCGVELLCD
VLFTPLCSPSLLNRLGGFSKPADVLRANLLHLNDVDDWSRWLSLTKVETADPSGGIFFSDMNLVFSAAIAGQGIAMGDSL
TGQTALSEGRLVRPFDIAVPAARSYYLVVDLAKSDHAVLFAFRDWLRSSLADAAADMQAARG

Sequences:

>Translated_302_residues
MRIPSTQALRALESFARHGTVWRAAEELHLTRSAVSHQLRLLERELGFDLLERVGKGVALTARGRRYAHDVHKALTMIGD
AGAQNRGGGIGGSFRVSCTPGFASLWLCTHIAEFQEMYQDVSLSIVTPRRLDDVSNPDVDAFIAFGDGNWPNCGVELLCD
VLFTPLCSPSLLNRLGGFSKPADVLRANLLHLNDVDDWSRWLSLTKVETADPSGGIFFSDMNLVFSAAIAGQGIAMGDSL
TGQTALSEGRLVRPFDIAVPAARSYYLVVDLAKSDHAVLFAFRDWLRSSLADAAADMQAARG
>Mature_302_residues
MRIPSTQALRALESFARHGTVWRAAEELHLTRSAVSHQLRLLERELGFDLLERVGKGVALTARGRRYAHDVHKALTMIGD
AGAQNRGGGIGGSFRVSCTPGFASLWLCTHIAEFQEMYQDVSLSIVTPRRLDDVSNPDVDAFIAFGDGNWPNCGVELLCD
VLFTPLCSPSLLNRLGGFSKPADVLRANLLHLNDVDDWSRWLSLTKVETADPSGGIFFSDMNLVFSAAIAGQGIAMGDSL
TGQTALSEGRLVRPFDIAVPAARSYYLVVDLAKSDHAVLFAFRDWLRSSLADAAADMQAARG

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=300, Percent_Identity=33.6666666666667, Blast_Score=177, Evalue=1e-45,
Organism=Escherichia coli, GI1788706, Length=294, Percent_Identity=29.2517006802721, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=26.9230769230769, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI145693193, Length=250, Percent_Identity=28, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI157672245, Length=160, Percent_Identity=28.75, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1786401, Length=300, Percent_Identity=23.3333333333333, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32819; Mature: 32819

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPSTQALRALESFARHGTVWRAAEELHLTRSAVSHQLRLLERELGFDLLERVGKGVAL
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEE
TARGRRYAHDVHKALTMIGDAGAQNRGGGIGGSFRVSCTPGFASLWLCTHIAEFQEMYQD
EECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
VSLSIVTPRRLDDVSNPDVDAFIAFGDGNWPNCGVELLCDVLFTPLCSPSLLNRLGGFSK
CCEEEECCHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCC
PADVLRANLLHLNDVDDWSRWLSLTKVETADPSGGIFFSDMNLVFSAAIAGQGIAMGDSL
CHHHHHHHHHCCCCCHHHHHHHHHHEEECCCCCCCEEEECHHHHHHHHHCCCCEECCCCC
TGQTALSEGRLVRPFDIAVPAARSYYLVVDLAKSDHAVLFAFRDWLRSSLADAAADMQAA
CCCHHHCCCCEECCCCCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHC
RG
CC
>Mature Secondary Structure
MRIPSTQALRALESFARHGTVWRAAEELHLTRSAVSHQLRLLERELGFDLLERVGKGVAL
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCEEE
TARGRRYAHDVHKALTMIGDAGAQNRGGGIGGSFRVSCTPGFASLWLCTHIAEFQEMYQD
EECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHC
VSLSIVTPRRLDDVSNPDVDAFIAFGDGNWPNCGVELLCDVLFTPLCSPSLLNRLGGFSK
CCEEEECCHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCC
PADVLRANLLHLNDVDDWSRWLSLTKVETADPSGGIFFSDMNLVFSAAIAGQGIAMGDSL
CHHHHHHHHHCCCCCHHHHHHHHHHEEECCCCCCCEEEECHHHHHHHHHCCCCEECCCCC
TGQTALSEGRLVRPFDIAVPAARSYYLVVDLAKSDHAVLFAFRDWLRSSLADAAADMQAA
CCCHHHCCCCEECCCCCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHC
RG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]