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The map label for this gene is rsh [H]

Identifier: 150395901

GI number: 150395901

Start: 728903

End: 731128

Strand: Direct

Name: rsh [H]

Synonym: Smed_0677

Alternate gene names: 150395901

Gene position: 728903-731128 (Clockwise)

Preceding gene: 150395900

Following gene: 150395902

Centisome position: 19.27

GC content: 60.65

Gene sequence:

>2226_bases
ATGATGCGCCAATACGAGCTCGTCGAGCGCGTGCAGAAATACAAGCCCGACGTCAACGAGGCCCTGCTGAACAAGGCCTA
TGTTTACGCCATGCAGAAGCATGGGCAGCAGAAGCGGGCAAGCGGCGATCCCTACATCTCCCATCCCCTGGAGGTCGCGG
CGATCCTGACCGAAATGCATCTCGACGAATCCACGATAGCCGTCGCGCTGCTGCACGACACGATCGAGGATACGACCGCG
ACGCGACAGGAAATCGACGGCCTCTTCGGCGAAGATATCGGTGCCTTGGTCGAAGGTCTCACCAAGATCAAGAAACTCGA
CCTCGTCACCAAGAAGGCCAAGCAGGCGGAAAATCTGCGCAAGCTGCTGCTCGCTATTTCGGACGACGTCCGCGTCCTCC
TGGTCAAGCTCGCCGATCGCCTCCACAATATGCGTACGCTCGATCACATGTCGGCGGAAAAGCGCGCGCGCATTTCGGAG
GAGACGATGGACATCTATGCGCCCCTGGCGGGGCGCATGGGCATGCAGGATATGCGCGAGGAGCTTGAAAACCTCTCCTT
TCGGCACATCAACCCGGAAGCCTACGAAACCGTCACCAGACGCCTTCAGGAGCTCTCCGAACGTAACGAAGGGCTGATCA
AGAAGATCGAGGAGGAACTGAGCGAATTGCTTCAGGCGGAGGGGCTTGCCGATGCCCAGGTCAAGGGGCGCCAGAAGAAG
CCTTACTCGGTTTTCCGCAAAATGCAGTCGAAATCGCTTTCCTTCGAGCAGCTCTCGGACGTCTGGGGCTTCCGTATCAT
CGTCAGCGACATTCCGGCCTGCTATCGCGCGCTGGGTATCGTGCACACCCGCTGGCGCGTCGTGCCCGGACGCTTCAAGG
ACTATATCTCGACGCCGAAGCAGAACGACTATCAATCCATTCATACGACGATCATCGGTCCGTCGCGCCAGCGCATCGAA
CTGCAAATCCGCACGAAGCGCATGCATGAGATCGCCGAATACGGTATCGCGGCCCACGCACTCTACAAGGATCGCGACAC
TGTTTCCGGCGACCTGACGCGCACACCGACATCGAACGCCTATTCCTGGCTGCGCCGCACGATCGAATCTCTCGCCGAGG
GTGATAATCCGGAGGAGTTCCTCGAGCACACCAAGCTCGAACTCTTCCAGGATCAGGTCTTCTGCTTCACTCCGAAGGGG
CAGCTGATAGCATTGCCGCGCGGTGCCACCCCGATCGACTTCGCCTATGCCGTGCATACGAATATCGGCGACACCTGCGT
CGGGGCCAAGATCAACGGTCGCATCATGCCCCTGGTGACGCGGCTCAACAATGGCGATGAAGTGGAGATCATCCGTTCGG
GCATTCAGGTGCCGCCGCCGGCCTGGGAAGAGATCGTCGTCACGGGCAAGGCTCGGGCGGCCATCCGCCGCGCTACTCGC
GCCGCGATCCGCAAGCAATATGCCGGCCTCGGCTATCGCATCCTGGAGCGCACTTTCGAACGGGCAGGGAAGACGTTCTC
GCGCGAGGCGTTGAAGCCGGTGCTGCATCGGCTCGGGCAAAAGGACGTGGAAGACGCCATCGCTGCGGTAGGACGCGGCG
AACTCTCCTCGCTCGACGTTCTGCGCGCCGTCTTCCCGGATCATCAGGACGAGCGCGTGACCGTGAAGCCTAGCGCCGAT
GACGGCTGGTTCAACATGCGCAGCGCCGCAGGCATGATCTTCAAGCTGCCGGAGCGGTCGAAGGAAATGGCCGCCGCAGA
GCAGGCGGAAGGCCCCGAAGCTCTCCCGATCCGCGGCCTGTCCGGCAATGCCGAGGTCCACTTCAGTCCTGCCGGCGCCG
TTCCCGGAGATCGCATCGTCGGTATTATGGACAAGGACAAAGGCATCACCATCTATCCGATCCAGTCGCCGATCCTGCAG
CAGTTCGACGAGCAGCCCGAGCGCTGGATCGACGTTCGTTGGGATCTCGACGAGGCGAACAACAGCCGTTTCATTGCCAG
AATTGCGGTCAGCGCCTTGAACGAGCCGGGTACGCTGGCGGAGGTCGCACAGGCGATCGCGACCACCGATGTCAACATCC
GCTCCCTATCGATGGGGCGCGTCGCGGCTGATTTCAGCGAATTGCAGTTCGACCTCGAAGTCTGGGATCTGCGTCAGCTC
AATCACCTGATGGCGCAACTCAAGGAATTGCCAAGCATTTCCATGGTCAAGCGCCTCTTCGAGTAG

Upstream 100 bases:

>100_bases
ATAGTTCCGGAACCATAGTTCCGACGCGTTATCGAAGCAGTGCGCCACTCGTGTGATTGGCGCACTTTTTTATTTGCCTG
ATTTCAGGAGCCGTCAGCGG

Downstream 100 bases:

>100_bases
GGCGTCTGCCGGGCGGCCACGCAGAGATGCTGCTCCTGCAGGCGCGCAAGGATGCGTTCCATTTCCGTGCAGACTGCCTT
CAAAATAAGCAGTTAGGGGC

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 741; Mature: 741

Protein sequence:

>741_residues
MMRQYELVERVQKYKPDVNEALLNKAYVYAMQKHGQQKRASGDPYISHPLEVAAILTEMHLDESTIAVALLHDTIEDTTA
TRQEIDGLFGEDIGALVEGLTKIKKLDLVTKKAKQAENLRKLLLAISDDVRVLLVKLADRLHNMRTLDHMSAEKRARISE
ETMDIYAPLAGRMGMQDMREELENLSFRHINPEAYETVTRRLQELSERNEGLIKKIEEELSELLQAEGLADAQVKGRQKK
PYSVFRKMQSKSLSFEQLSDVWGFRIIVSDIPACYRALGIVHTRWRVVPGRFKDYISTPKQNDYQSIHTTIIGPSRQRIE
LQIRTKRMHEIAEYGIAAHALYKDRDTVSGDLTRTPTSNAYSWLRRTIESLAEGDNPEEFLEHTKLELFQDQVFCFTPKG
QLIALPRGATPIDFAYAVHTNIGDTCVGAKINGRIMPLVTRLNNGDEVEIIRSGIQVPPPAWEEIVVTGKARAAIRRATR
AAIRKQYAGLGYRILERTFERAGKTFSREALKPVLHRLGQKDVEDAIAAVGRGELSSLDVLRAVFPDHQDERVTVKPSAD
DGWFNMRSAAGMIFKLPERSKEMAAAEQAEGPEALPIRGLSGNAEVHFSPAGAVPGDRIVGIMDKDKGITIYPIQSPILQ
QFDEQPERWIDVRWDLDEANNSRFIARIAVSALNEPGTLAEVAQAIATTDVNIRSLSMGRVAADFSELQFDLEVWDLRQL
NHLMAQLKELPSISMVKRLFE

Sequences:

>Translated_741_residues
MMRQYELVERVQKYKPDVNEALLNKAYVYAMQKHGQQKRASGDPYISHPLEVAAILTEMHLDESTIAVALLHDTIEDTTA
TRQEIDGLFGEDIGALVEGLTKIKKLDLVTKKAKQAENLRKLLLAISDDVRVLLVKLADRLHNMRTLDHMSAEKRARISE
ETMDIYAPLAGRMGMQDMREELENLSFRHINPEAYETVTRRLQELSERNEGLIKKIEEELSELLQAEGLADAQVKGRQKK
PYSVFRKMQSKSLSFEQLSDVWGFRIIVSDIPACYRALGIVHTRWRVVPGRFKDYISTPKQNDYQSIHTTIIGPSRQRIE
LQIRTKRMHEIAEYGIAAHALYKDRDTVSGDLTRTPTSNAYSWLRRTIESLAEGDNPEEFLEHTKLELFQDQVFCFTPKG
QLIALPRGATPIDFAYAVHTNIGDTCVGAKINGRIMPLVTRLNNGDEVEIIRSGIQVPPPAWEEIVVTGKARAAIRRATR
AAIRKQYAGLGYRILERTFERAGKTFSREALKPVLHRLGQKDVEDAIAAVGRGELSSLDVLRAVFPDHQDERVTVKPSAD
DGWFNMRSAAGMIFKLPERSKEMAAAEQAEGPEALPIRGLSGNAEVHFSPAGAVPGDRIVGIMDKDKGITIYPIQSPILQ
QFDEQPERWIDVRWDLDEANNSRFIARIAVSALNEPGTLAEVAQAIATTDVNIRSLSMGRVAADFSELQFDLEVWDLRQL
NHLMAQLKELPSISMVKRLFE
>Mature_741_residues
MMRQYELVERVQKYKPDVNEALLNKAYVYAMQKHGQQKRASGDPYISHPLEVAAILTEMHLDESTIAVALLHDTIEDTTA
TRQEIDGLFGEDIGALVEGLTKIKKLDLVTKKAKQAENLRKLLLAISDDVRVLLVKLADRLHNMRTLDHMSAEKRARISE
ETMDIYAPLAGRMGMQDMREELENLSFRHINPEAYETVTRRLQELSERNEGLIKKIEEELSELLQAEGLADAQVKGRQKK
PYSVFRKMQSKSLSFEQLSDVWGFRIIVSDIPACYRALGIVHTRWRVVPGRFKDYISTPKQNDYQSIHTTIIGPSRQRIE
LQIRTKRMHEIAEYGIAAHALYKDRDTVSGDLTRTPTSNAYSWLRRTIESLAEGDNPEEFLEHTKLELFQDQVFCFTPKG
QLIALPRGATPIDFAYAVHTNIGDTCVGAKINGRIMPLVTRLNNGDEVEIIRSGIQVPPPAWEEIVVTGKARAAIRRATR
AAIRKQYAGLGYRILERTFERAGKTFSREALKPVLHRLGQKDVEDAIAAVGRGELSSLDVLRAVFPDHQDERVTVKPSAD
DGWFNMRSAAGMIFKLPERSKEMAAAEQAEGPEALPIRGLSGNAEVHFSPAGAVPGDRIVGIMDKDKGITIYPIQSPILQ
QFDEQPERWIDVRWDLDEANNSRFIARIAVSALNEPGTLAEVAQAIATTDVNIRSLSMGRVAADFSELQFDLEVWDLRQL
NHLMAQLKELPSISMVKRLFE

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=137, Percent_Identity=40.1459854014599, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1790082, Length=738, Percent_Identity=37.2628726287263, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1789147, Length=734, Percent_Identity=30.7901907356948, Blast_Score=362, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI25143118, Length=130, Percent_Identity=38.4615384615385, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24650996, Length=123, Percent_Identity=39.0243902439024, Blast_Score=79, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 83621; Mature: 83621

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRQYELVERVQKYKPDVNEALLNKAYVYAMQKHGQQKRASGDPYISHPLEVAAILTEMH
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHH
LDESTIAVALLHDTIEDTTATRQEIDGLFGEDIGALVEGLTKIKKLDLVTKKAKQAENLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLLAISDDVRVLLVKLADRLHNMRTLDHMSAEKRARISEETMDIYAPLAGRMGMQDMRE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ELENLSFRHINPEAYETVTRRLQELSERNEGLIKKIEEELSELLQAEGLADAQVKGRQKK
HHHCCCEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
PYSVFRKMQSKSLSFEQLSDVWGFRIIVSDIPACYRALGIVHTRWRVVPGRFKDYISTPK
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHCCCC
QNDYQSIHTTIIGPSRQRIELQIRTKRMHEIAEYGIAAHALYKDRDTVSGDLTRTPTSNA
CCCHHHHHHEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
YSWLRRTIESLAEGDNPEEFLEHTKLELFQDQVFCFTPKGQLIALPRGATPIDFAYAVHT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCEEEEEEEC
NIGDTCVGAKINGRIMPLVTRLNNGDEVEIIRSGIQVPPPAWEEIVVTGKARAAIRRATR
CCCCCEECCEECCEEEEEEEECCCCCHHHHHHCCCCCCCCCCHHEEEECHHHHHHHHHHH
AAIRKQYAGLGYRILERTFERAGKTFSREALKPVLHRLGQKDVEDAIAAVGRGELSSLDV
HHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHH
LRAVFPDHQDERVTVKPSADDGWFNMRSAAGMIFKLPERSKEMAAAEQAEGPEALPIRGL
HHHHCCCCCCCEEEEECCCCCCCCHHHHHCCEEEECCCHHHHHHHHHHCCCCCCCEEECC
SGNAEVHFSPAGAVPGDRIVGIMDKDKGITIYPIQSPILQQFDEQPERWIDVRWDLDEAN
CCCCEEEECCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHCCCCCEEEEEEECCCCC
NSRFIARIAVSALNEPGTLAEVAQAIATTDVNIRSLSMGRVAADFSELQFDLEVWDLRQL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHEEHHHHHHHHHH
NHLMAQLKELPSISMVKRLFE
HHHHHHHHHCCCHHHHHHHHC
>Mature Secondary Structure
MMRQYELVERVQKYKPDVNEALLNKAYVYAMQKHGQQKRASGDPYISHPLEVAAILTEMH
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHHHH
LDESTIAVALLHDTIEDTTATRQEIDGLFGEDIGALVEGLTKIKKLDLVTKKAKQAENLR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLLLAISDDVRVLLVKLADRLHNMRTLDHMSAEKRARISEETMDIYAPLAGRMGMQDMRE
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
ELENLSFRHINPEAYETVTRRLQELSERNEGLIKKIEEELSELLQAEGLADAQVKGRQKK
HHHCCCEECCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCC
PYSVFRKMQSKSLSFEQLSDVWGFRIIVSDIPACYRALGIVHTRWRVVPGRFKDYISTPK
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHCCCC
QNDYQSIHTTIIGPSRQRIELQIRTKRMHEIAEYGIAAHALYKDRDTVSGDLTRTPTSNA
CCCHHHHHHEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHH
YSWLRRTIESLAEGDNPEEFLEHTKLELFQDQVFCFTPKGQLIALPRGATPIDFAYAVHT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEECCCCCCCEEEEEEEC
NIGDTCVGAKINGRIMPLVTRLNNGDEVEIIRSGIQVPPPAWEEIVVTGKARAAIRRATR
CCCCCEECCEECCEEEEEEEECCCCCHHHHHHCCCCCCCCCCHHEEEECHHHHHHHHHHH
AAIRKQYAGLGYRILERTFERAGKTFSREALKPVLHRLGQKDVEDAIAAVGRGELSSLDV
HHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHH
LRAVFPDHQDERVTVKPSADDGWFNMRSAAGMIFKLPERSKEMAAAEQAEGPEALPIRGL
HHHHCCCCCCCEEEEECCCCCCCCHHHHHCCEEEECCCHHHHHHHHHHCCCCCCCEEECC
SGNAEVHFSPAGAVPGDRIVGIMDKDKGITIYPIQSPILQQFDEQPERWIDVRWDLDEAN
CCCCEEEECCCCCCCCCCEEEEEECCCCEEEEECCCHHHHHHHCCCCCEEEEEEECCCCC
NSRFIARIAVSALNEPGTLAEVAQAIATTDVNIRSLSMGRVAADFSELQFDLEVWDLRQL
CCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHEEHHHHHHHHHH
NHLMAQLKELPSISMVKRLFE
HHHHHHHHHCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA