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The map label for this gene is mrpD [H]

Identifier: 150395834

GI number: 150395834

Start: 658991

End: 660553

Strand: Direct

Name: mrpD [H]

Synonym: Smed_0610

Alternate gene names: 150395834

Gene position: 658991-660553 (Clockwise)

Preceding gene: 150395833

Following gene: 150395835

Centisome position: 17.42

GC content: 62.12

Gene sequence:

>1563_bases
ATGGCTGTTTCTACCTCCGCTCCCGTCGACCTTTCCGCAGCACTGGTGCTGACCCCCACATCGGCTGCCGAATGGCTTGT
CGTTCTGCCGGTCGCGTGGTGCCTGGCTGTCGGCGCGCTCCTCATGATGCTTCGCCGATGGGCCATTCTCCAGCCTGTGA
TAGCCCTGGCGGCTCTGGCCGTGCTCGTGCTGATCGACGTGGCGCTGCTCGATCATGTCGCGCGAAACGGTCCGACGACG
ATGGTGATGGGACGCTGGCTGCCGCCGTTCGGTATCGCCTTCACCGTCGACCTGACCGGAGCGCTCTTTGCTCTTATGGC
CGCTGCCGTTGCGTTTGCCGCCGGCATCTTTTCGCTGGCCGATATCAACGAGAGCGGCCGGCGCTACGGTTTCTATCCGC
TGCTGATGCTGCTGATGGCCGGCGTTTCGGGAGCCTTCCTTACGGGAGATATCTTCAATCTCTATGTCTGGTTCGAGGTC
CTGCTGATTTCGTCCTTCGGTCTTCTGGTCCTCGGCTCCGAGAGGGAGCAGATCGACGGGACGGTGAAATACGGATTTCT
GAATCTGGTCGCGACGACCTTGTTTCTCGTCGCCACCGGCTTCCTCTACGCCGTGTTCGGCACGCTCAACATGGCCGACA
TCGCGCGCAAGGCTGACGAACTGCGCGCCGGTGCGCCGCTGATGACATTGGTCGGGCTCTATGTTCTTGCCTTCGGCATG
AAGGCGGCCGCGTTTCCAGTGAATTTCTGGCTGCCGGCTTCCTATCATACGCCTCGCGTGGTCGTGTCGGCGCTCTTTGC
AGGCCTGCTTACCAAGGTCGGTATTTATGCCCTGATCCGGGTGACTGTCATGCTCTTCCCGGTCGAAAGGGAGGAGCTGA
GCTTTGTACTGGCGCTTATCGGCGCGCTGACGATGATGGTCGGCGTTCTCGGTGCGCTCGCGCAAACGGATTTCCGCCGC
ATCCTCGGCTACCTCGTCGTGTCGGGCATCGGCTCGATGGTTGCAGGCATCGCCATCGGCGGACCAGGCGGCATCGGCGG
CGCAATTTTCTATGCACTCCATTCGATGCTGGTGATGACGGGCCTGTATTTTGCTTCAGGGATTGCCATGCGCCTTGGCG
CGGATAGCTCGATCTCGACGCTTGCCGGTCTTTACCGCAAGCATGTCGGATTTGCTGCGCTCACCTTGATGCTGTGCTTC
GCCGTTTCGGGCCTCCCGCCCTTTTCCGGCTTCTGGCCGAAGGTCATGCTGGTGAAGGCAGCGCTCGACATCGGCGCCTG
GTGGCTTGCCGCCGCGCTCCTTCTTGTCGGCTTCTTCACCATGATAGCCACGGGCCGGCTTTTCCTCCTGGCCTATTGGC
GCGAGAGTACGAGACCTGACGCCGGCGGTACGACGCGCTTGCCGGCCAAGACTTTCGGTCCGCTTGCCGCGCTTACGCTG
CTGGCGCTGGCGCTTGGCGTCTACCCCGAGCCGCTGCTTGCGATGATCCAGAACGCGGCGGCTGGTCTGGCCGAGCCCTC
CGCTTACCTGAATTCAGTCTTTCCCGCCGGAGGTCCGCAATGA

Upstream 100 bases:

>100_bases
CTGGCGGGCGTATGAAGACCTGGCGACGGACAATACGGACGAGATGCGAGTTGCGGAACCGGTGAGCGAGCCGGCGCCGC
CGCTGGGATACTGACGCGCC

Downstream 100 bases:

>100_bases
AGTTCCTGCTGACCAACGTAATTTTCACGCTGATCTGGGGAGCAATCAGCGCCAGCTTCACGCCCGCGAACCTATTGCTG
GGCTTCGCAGCCGGCGCCCT

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: Mrp complex subunit D; Multiple resistance and pH homeostasis protein D [H]

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MAVSTSAPVDLSAALVLTPTSAAEWLVVLPVAWCLAVGALLMMLRRWAILQPVIALAALAVLVLIDVALLDHVARNGPTT
MVMGRWLPPFGIAFTVDLTGALFALMAAAVAFAAGIFSLADINESGRRYGFYPLLMLLMAGVSGAFLTGDIFNLYVWFEV
LLISSFGLLVLGSEREQIDGTVKYGFLNLVATTLFLVATGFLYAVFGTLNMADIARKADELRAGAPLMTLVGLYVLAFGM
KAAAFPVNFWLPASYHTPRVVVSALFAGLLTKVGIYALIRVTVMLFPVEREELSFVLALIGALTMMVGVLGALAQTDFRR
ILGYLVVSGIGSMVAGIAIGGPGGIGGAIFYALHSMLVMTGLYFASGIAMRLGADSSISTLAGLYRKHVGFAALTLMLCF
AVSGLPPFSGFWPKVMLVKAALDIGAWWLAAALLLVGFFTMIATGRLFLLAYWRESTRPDAGGTTRLPAKTFGPLAALTL
LALALGVYPEPLLAMIQNAAAGLAEPSAYLNSVFPAGGPQ

Sequences:

>Translated_520_residues
MAVSTSAPVDLSAALVLTPTSAAEWLVVLPVAWCLAVGALLMMLRRWAILQPVIALAALAVLVLIDVALLDHVARNGPTT
MVMGRWLPPFGIAFTVDLTGALFALMAAAVAFAAGIFSLADINESGRRYGFYPLLMLLMAGVSGAFLTGDIFNLYVWFEV
LLISSFGLLVLGSEREQIDGTVKYGFLNLVATTLFLVATGFLYAVFGTLNMADIARKADELRAGAPLMTLVGLYVLAFGM
KAAAFPVNFWLPASYHTPRVVVSALFAGLLTKVGIYALIRVTVMLFPVEREELSFVLALIGALTMMVGVLGALAQTDFRR
ILGYLVVSGIGSMVAGIAIGGPGGIGGAIFYALHSMLVMTGLYFASGIAMRLGADSSISTLAGLYRKHVGFAALTLMLCF
AVSGLPPFSGFWPKVMLVKAALDIGAWWLAAALLLVGFFTMIATGRLFLLAYWRESTRPDAGGTTRLPAKTFGPLAALTL
LALALGVYPEPLLAMIQNAAAGLAEPSAYLNSVFPAGGPQ
>Mature_519_residues
AVSTSAPVDLSAALVLTPTSAAEWLVVLPVAWCLAVGALLMMLRRWAILQPVIALAALAVLVLIDVALLDHVARNGPTTM
VMGRWLPPFGIAFTVDLTGALFALMAAAVAFAAGIFSLADINESGRRYGFYPLLMLLMAGVSGAFLTGDIFNLYVWFEVL
LISSFGLLVLGSEREQIDGTVKYGFLNLVATTLFLVATGFLYAVFGTLNMADIARKADELRAGAPLMTLVGLYVLAFGMK
AAAFPVNFWLPASYHTPRVVVSALFAGLLTKVGIYALIRVTVMLFPVEREELSFVLALIGALTMMVGVLGALAQTDFRRI
LGYLVVSGIGSMVAGIAIGGPGGIGGAIFYALHSMLVMTGLYFASGIAMRLGADSSISTLAGLYRKHVGFAALTLMLCFA
VSGLPPFSGFWPKVMLVKAALDIGAWWLAAALLLVGFFTMIATGRLFLLAYWRESTRPDAGGTTRLPAKTFGPLAALTLL
ALALGVYPEPLLAMIQNAAAGLAEPSAYLNSVFPAGGPQ

Specific function: Mrp complex is a Na(+)/H(+) antiporter involved in Na(+) excretion and Na(+) resistance [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=227, Percent_Identity=24.2290748898678, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI1788613, Length=355, Percent_Identity=25.3521126760563, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1788827, Length=351, Percent_Identity=24.2165242165242, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1788831, Length=399, Percent_Identity=25.062656641604, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI145693160, Length=394, Percent_Identity=22.8426395939086, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788829, Length=332, Percent_Identity=23.7951807228916, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788614, Length=279, Percent_Identity=29.3906810035842, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 54837; Mature: 54706

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVSTSAPVDLSAALVLTPTSAAEWLVVLPVAWCLAVGALLMMLRRWAILQPVIALAALA
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVLIDVALLDHVARNGPTTMVMGRWLPPFGIAFTVDLTGALFALMAAAVAFAAGIFSLA
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
DINESGRRYGFYPLLMLLMAGVSGAFLTGDIFNLYVWFEVLLISSFGLLVLGSEREQIDG
HCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHCC
TVKYGFLNLVATTLFLVATGFLYAVFGTLNMADIARKADELRAGAPLMTLVGLYVLAFGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
KAAAFPVNFWLPASYHTPRVVVSALFAGLLTKVGIYALIRVTVMLFPVEREELSFVLALI
HHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GALTMMVGVLGALAQTDFRRILGYLVVSGIGSMVAGIAIGGPGGIGGAIFYALHSMLVMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GLYFASGIAMRLGADSSISTLAGLYRKHVGFAALTLMLCFAVSGLPPFSGFWPKVMLVKA
HHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ALDIGAWWLAAALLLVGFFTMIATGRLFLLAYWRESTRPDAGGTTRLPAKTFGPLAALTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LALALGVYPEPLLAMIQNAAAGLAEPSAYLNSVFPAGGPQ
HHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
AVSTSAPVDLSAALVLTPTSAAEWLVVLPVAWCLAVGALLMMLRRWAILQPVIALAALA
CCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLVLIDVALLDHVARNGPTTMVMGRWLPPFGIAFTVDLTGALFALMAAAVAFAAGIFSLA
HHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
DINESGRRYGFYPLLMLLMAGVSGAFLTGDIFNLYVWFEVLLISSFGLLVLGSEREQIDG
HCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHCC
TVKYGFLNLVATTLFLVATGFLYAVFGTLNMADIARKADELRAGAPLMTLVGLYVLAFGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
KAAAFPVNFWLPASYHTPRVVVSALFAGLLTKVGIYALIRVTVMLFPVEREELSFVLALI
HHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GALTMMVGVLGALAQTDFRRILGYLVVSGIGSMVAGIAIGGPGGIGGAIFYALHSMLVMT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
GLYFASGIAMRLGADSSISTLAGLYRKHVGFAALTLMLCFAVSGLPPFSGFWPKVMLVKA
HHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
ALDIGAWWLAAALLLVGFFTMIATGRLFLLAYWRESTRPDAGGTTRLPAKTFGPLAALTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
LALALGVYPEPLLAMIQNAAAGLAEPSAYLNSVFPAGGPQ
HHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11356194 [H]