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The map label for this gene is leuB [H]

Identifier: 150401838

GI number: 150401838

Start: 1472184

End: 1473176

Strand: Direct

Name: leuB [H]

Synonym: Maeo_1416

Alternate gene names: 150401838

Gene position: 1472184-1473176 (Clockwise)

Preceding gene: 150401835

Following gene: 150401839

Centisome position: 93.8

GC content: 35.55

Gene sequence:

>993_bases
ATGCATAAAATTTGTGTTATAGAAGGAGATGGAATTGGTAAGGAAGTAGTTCCAGCAGCAGTATCCATATTAAAGGCAAC
AGGCGTAGATTTTGAATTTATAAATGCAGATGCAGGGGACGAAGTATTTGAAAAAACAGGAGTTGCTTTGCCAGAGGAAA
CAGTGAAATCCGCAAAAGAATGCGATGCCGTATTTTTTGGTGCAGCGGGAGAAACAGCCGCCGATGTAATCGTAAAATTA
AGAAAAATATTAAATACTTATGCAAATGTTAGGCCTGTAAAGGCATACAAAGGTATAAACTGTTTAAATAGCGATATTGA
TTATATAATTGTAAGGGAAAACACAGAAGGATTATATAAAGGAATAGAGGCAGAAATAGCAGATGGAGTTTATACGGCTA
CAAGAGTAATTACAGAAGAGGCATGCGAAAAAATATTTAAATTTGCATGTAATATGGCAGAGGATAGGAAAAAACAAGGT
ATAAATAACGGCGAAATAACATGTGGTCATAAAGCTAATGTGTTAAAATTAACCGATGGAGTATTTAAAAATAAATTTTA
TGAAGTTGCTAAACAATACTCAGTAAAACCAGAGGATTATTATGTTGATGCCTTAAATATGTATCTAATAACAAGACCAG
AAACATTTAATGTGGTTGTTACCTCAAACCTTTTTGGAGATATTTTATCAGATGGTGCAGCTGGAACTGTTGGAGGGCTT
GGTATGGCTCCATCGGCAAACATCGGAGATGAATACGGATTATTTGAACCGGTTCATGGTTCAGCACCAGACATAGCAGG
TAAAGGAATAGCTAACCCAACAGCTACAATTTTAACGGCAATATTAATGTTAAGATATTTAAATGAAAATGATGCAGCTG
ATAGGGTGGAAAAGGCACTTGAAGAAGTTCTTGAAAACAAATTAACCACGCCAGATTTAGGGGGAAGCTTAACAACGACA
CAAATGGCAGAAGAAGTTGCTAAAAGAGTTTAA

Upstream 100 bases:

>100_bases
ATAATGATATTTTTATATTATTTTCCATATTATTTTATATTATTATTTCCTATTTATAATATATATTAATATTTATTTTA
ATCAAAATCAAGGTGAAATT

Downstream 100 bases:

>100_bases
TTTATTCCAATAAATAAAAAATATAAATGGTGATTAAATGTATAATGCTACAATAATTATAAAATTGAAAAAAGGAGTAT
TAAACCCAGAAGGTAGAACA

Product: multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase

Products: NA

Alternate protein names: 3-isopropylmalate dehydrogenase; 3-IPM-DH; IMDH; IPMDH; Beta-IPM dehydrogenase; D-malate dehydrogenase [decarboxylating] [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MHKICVIEGDGIGKEVVPAAVSILKATGVDFEFINADAGDEVFEKTGVALPEETVKSAKECDAVFFGAAGETAADVIVKL
RKILNTYANVRPVKAYKGINCLNSDIDYIIVRENTEGLYKGIEAEIADGVYTATRVITEEACEKIFKFACNMAEDRKKQG
INNGEITCGHKANVLKLTDGVFKNKFYEVAKQYSVKPEDYYVDALNMYLITRPETFNVVVTSNLFGDILSDGAAGTVGGL
GMAPSANIGDEYGLFEPVHGSAPDIAGKGIANPTATILTAILMLRYLNENDAADRVEKALEEVLENKLTTPDLGGSLTTT
QMAEEVAKRV

Sequences:

>Translated_330_residues
MHKICVIEGDGIGKEVVPAAVSILKATGVDFEFINADAGDEVFEKTGVALPEETVKSAKECDAVFFGAAGETAADVIVKL
RKILNTYANVRPVKAYKGINCLNSDIDYIIVRENTEGLYKGIEAEIADGVYTATRVITEEACEKIFKFACNMAEDRKKQG
INNGEITCGHKANVLKLTDGVFKNKFYEVAKQYSVKPEDYYVDALNMYLITRPETFNVVVTSNLFGDILSDGAAGTVGGL
GMAPSANIGDEYGLFEPVHGSAPDIAGKGIANPTATILTAILMLRYLNENDAADRVEKALEEVLENKLTTPDLGGSLTTT
QMAEEVAKRV
>Mature_330_residues
MHKICVIEGDGIGKEVVPAAVSILKATGVDFEFINADAGDEVFEKTGVALPEETVKSAKECDAVFFGAAGETAADVIVKL
RKILNTYANVRPVKAYKGINCLNSDIDYIIVRENTEGLYKGIEAEIADGVYTATRVITEEACEKIFKFACNMAEDRKKQG
INNGEITCGHKANVLKLTDGVFKNKFYEVAKQYSVKPEDYYVDALNMYLITRPETFNVVVTSNLFGDILSDGAAGTVGGL
GMAPSANIGDEYGLFEPVHGSAPDIAGKGIANPTATILTAILMLRYLNENDAADRVEKALEEVLENKLTTPDLGGSLTTT
QMAEEVAKRV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate, which decarboxylates to 4-methyl-2-oxopentanoate (2-oxoisocaproate). Also catalyzes the oxidative decarboxylation of 3-methylmal

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=339, Percent_Identity=36.8731563421829, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4758582, Length=336, Percent_Identity=33.9285714285714, Blast_Score=181, Evalue=8e-46,
Organism=Homo sapiens, GI28178838, Length=312, Percent_Identity=33.974358974359, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI28178821, Length=334, Percent_Identity=34.4311377245509, Blast_Score=162, Evalue=3e-40,
Organism=Homo sapiens, GI28178816, Length=334, Percent_Identity=34.1317365269461, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI28178819, Length=206, Percent_Identity=36.4077669902913, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1788101, Length=357, Percent_Identity=35.2941176470588, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87081683, Length=362, Percent_Identity=35.6353591160221, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787381, Length=366, Percent_Identity=30.8743169398907, Blast_Score=147, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI71986051, Length=349, Percent_Identity=35.8166189111748, Blast_Score=205, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17550882, Length=343, Percent_Identity=35.5685131195335, Blast_Score=180, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI25144293, Length=341, Percent_Identity=34.3108504398827, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17505779, Length=329, Percent_Identity=33.434650455927, Blast_Score=167, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6322097, Length=349, Percent_Identity=44.6991404011461, Blast_Score=248, Evalue=8e-67,
Organism=Saccharomyces cerevisiae, GI6324291, Length=337, Percent_Identity=37.3887240356083, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6324709, Length=341, Percent_Identity=34.6041055718475, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6319830, Length=366, Percent_Identity=34.4262295081967, Blast_Score=169, Evalue=5e-43,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=37.4251497005988, Blast_Score=232, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24643268, Length=334, Percent_Identity=37.4251497005988, Blast_Score=231, Evalue=4e-61,
Organism=Drosophila melanogaster, GI24661184, Length=339, Percent_Identity=32.7433628318584, Blast_Score=201, Evalue=5e-52,
Organism=Drosophila melanogaster, GI161078635, Length=342, Percent_Identity=30.4093567251462, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI161078633, Length=342, Percent_Identity=30.4093567251462, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24650122, Length=342, Percent_Identity=30.4093567251462, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI161078637, Length=342, Percent_Identity=30.4093567251462, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI161078639, Length=338, Percent_Identity=30.4733727810651, Blast_Score=162, Evalue=2e-40,
Organism=Drosophila melanogaster, GI281362242, Length=333, Percent_Identity=32.1321321321321, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI24648872, Length=333, Percent_Identity=32.1321321321321, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI20130355, Length=328, Percent_Identity=28.9634146341463, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85; =1.1.1.83 [H]

Molecular weight: Translated: 35555; Mature: 35555

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKICVIEGDGIGKEVVPAAVSILKATGVDFEFINADAGDEVFEKTGVALPEETVKSAKE
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHH
CDAVFFGAAGETAADVIVKLRKILNTYANVRPVKAYKGINCLNSDIDYIIVRENTEGLYK
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHCCCCCCEEEEEECCHHHHH
GIEAEIADGVYTATRVITEEACEKIFKFACNMAEDRKKQGINNGEITCGHKANVLKLTDG
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEECCCCCEEEECHH
VFKNKFYEVAKQYSVKPEDYYVDALNMYLITRPETFNVVVTSNLFGDILSDGAAGTVGGL
HHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCEEEEEEECHHHHHHHCCCCCCCCCCC
GMAPSANIGDEYGLFEPVHGSAPDIAGKGIANPTATILTAILMLRYLNENDAADRVEKAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EEVLENKLTTPDLGGSLTTTQMAEEVAKRV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MHKICVIEGDGIGKEVVPAAVSILKATGVDFEFINADAGDEVFEKTGVALPEETVKSAKE
CCEEEEEECCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCCCHHHHHHHHH
CDAVFFGAAGETAADVIVKLRKILNTYANVRPVKAYKGINCLNSDIDYIIVRENTEGLYK
CCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCHHHHCCCHHCCCCCCEEEEEECCHHHHH
GIEAEIADGVYTATRVITEEACEKIFKFACNMAEDRKKQGINNGEITCGHKANVLKLTDG
CCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCEEECCCCCEEEECHH
VFKNKFYEVAKQYSVKPEDYYVDALNMYLITRPETFNVVVTSNLFGDILSDGAAGTVGGL
HHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCEEEEEEECHHHHHHHCCCCCCCCCCC
GMAPSANIGDEYGLFEPVHGSAPDIAGKGIANPTATILTAILMLRYLNENDAADRVEKAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
EEVLENKLTTPDLGGSLTTTQMAEEVAKRV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 10940051 [H]