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The map label for this gene is hycG [C]

Identifier: 150401781

GI number: 150401781

Start: 1421208

End: 1421648

Strand: Direct

Name: hycG [C]

Synonym: Maeo_1359

Alternate gene names: 150401781

Gene position: 1421208-1421648 (Clockwise)

Preceding gene: 150401780

Following gene: 150401782

Centisome position: 90.55

GC content: 37.64

Gene sequence:

>441_bases
ATGATAGAAAAAACTGCCCGTAAAAAAAATATACATATTATGGTGGCATACACAGGAGGCTGTAATGGCTGTGATATAGA
TATTGTAAATTGTGTTTTGTCTCCATTTTATGATGCCGAACAATACAATGTATTTTTAACATGGAATCCCAGGGAGGCAG
ATATTTTATTGGTTAGTGGCTGTGTAACAAAGGTAATGACAGAACCCCTTAAAGAAATATATGAGGCAATACCTGAACCA
AAGGCAGTAGTTGCAGTAGGTTGCTGTGCCTTGATGGGTGGAGTATATGCTAATATCGGGGGAGATTTGGGAACTTCTGA
TTTCATAGACGGGCCTGTTAAAAATATAATTCCTGTTGATATAAATGTTCCGGGCTGTTCTCCAAGACCAGAGGACATAA
TACAGGGAATAGTTCAAGCTTTACCGAAGATTTTAGAATAA

Upstream 100 bases:

>100_bases
AACTATTATACATATATTATTTTAATATTTAATGGTCATATTTGATATTGTATTAAAAATAATAGAAATAATAATAAAAT
AAAAATAAAGGAGGTGCGTT

Downstream 100 bases:

>100_bases
TATGGGAAAATATATTTAGTGAATATTGGTGATTTTATGATTGCTTATAATATGTCATATCTTTATGGAATAGAATTAAT
ACTGTCAATAGTAGGAATTC

Product: NADH ubiquinone oxidoreductase 20 kDa subunit

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MIEKTARKKNIHIMVAYTGGCNGCDIDIVNCVLSPFYDAEQYNVFLTWNPREADILLVSGCVTKVMTEPLKEIYEAIPEP
KAVVAVGCCALMGGVYANIGGDLGTSDFIDGPVKNIIPVDINVPGCSPRPEDIIQGIVQALPKILE

Sequences:

>Translated_146_residues
MIEKTARKKNIHIMVAYTGGCNGCDIDIVNCVLSPFYDAEQYNVFLTWNPREADILLVSGCVTKVMTEPLKEIYEAIPEP
KAVVAVGCCALMGGVYANIGGDLGTSDFIDGPVKNIIPVDINVPGCSPRPEDIIQGIVQALPKILE
>Mature_146_residues
MIEKTARKKNIHIMVAYTGGCNGCDIDIVNCVLSPFYDAEQYNVFLTWNPREADILLVSGCVTKVMTEPLKEIYEAIPEP
KAVVAVGCCALMGGVYANIGGDLGTSDFIDGPVKNIIPVDINVPGCSPRPEDIIQGIVQALPKILE

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=139, Percent_Identity=33.0935251798561, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI1789074, Length=134, Percent_Identity=35.8208955223881, Blast_Score=99, Evalue=9e-23,
Organism=Escherichia coli, GI1788834, Length=134, Percent_Identity=35.0746268656716, Blast_Score=97, Evalue=5e-22,
Organism=Escherichia coli, GI1788624, Length=124, Percent_Identity=33.0645161290323, Blast_Score=68, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17509685, Length=127, Percent_Identity=37.007874015748, Blast_Score=86, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24651058, Length=127, Percent_Identity=35.4330708661417, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI18859983, Length=127, Percent_Identity=34.6456692913386, Blast_Score=86, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24642371, Length=127, Percent_Identity=34.6456692913386, Blast_Score=86, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: NA

Molecular weight: Translated: 15740; Mature: 15740

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
4.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKTARKKNIHIMVAYTGGCNGCDIDIVNCVLSPFYDAEQYNVFLTWNPREADILLVSG
CCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEHH
CVTKVMTEPLKEIYEAIPEPKAVVAVGCCALMGGVYANIGGDLGTSDFIDGPVKNIIPVD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEEEE
INVPGCSPRPEDIIQGIVQALPKILE
EECCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIEKTARKKNIHIMVAYTGGCNGCDIDIVNCVLSPFYDAEQYNVFLTWNPREADILLVSG
CCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCEEEEHH
CVTKVMTEPLKEIYEAIPEPKAVVAVGCCALMGGVYANIGGDLGTSDFIDGPVKNIIPVD
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCEEEEE
INVPGCSPRPEDIIQGIVQALPKILE
EECCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]