Click here to switch to the map view.

The map label for this gene is ygfT [C]

Identifier: 150401595

GI number: 150401595

Start: 1237376

End: 1237831

Strand: Reverse

Name: ygfT [C]

Synonym: Maeo_1172

Alternate gene names: 150401595

Gene position: 1237831-1237376 (Counterclockwise)

Preceding gene: 150401596

Following gene: 150401594

Centisome position: 78.87

GC content: 30.92

Gene sequence:

>456_bases
ATGAATCCAAAATTAATAGTTCTTGACCCATCAAAATGCACAAACTGTGATGATTGTGTCAATAAATGTAAAGAAATTCA
TGGGATAAGTAGGATAAAAAAAGCAAATAATGTTCCTGTATTTTGTATGCAGTGTGAAAATGCACCTTGCTATAATATTT
GTCCCGTAGATGCCATTTATTTAAAAGATGGGATACCACTTGTAAAAAAAGAAAGATGTATTGGTTGTGGCATGTGTGAA
ATTGTCTGTCCTGTTGGTTCAATATTCATTGATAAATTATATGCCCATAAATGTTCACTTTGCTTAGATACTGATAGAAT
AACTCCTGCATGTCTTGAAGCCTGTAAAGATAAGGCTTTAAAACTAGTATGCGATGAATGTATCGATTCAATAAAAGAAG
AAAGAAGAAAAAAACTAATGAAAATAATATCTGATGAATGGAGAGAGGATATTTAA

Upstream 100 bases:

>100_bases
TAAAGAATAATTACCAATGTTATTAATATTACGATACATATATTATTATTTATAAATAATAATATATAATACCAAATAAT
TCATTGAATCTGGTGAAATT

Downstream 100 bases:

>100_bases
TCGATATTTTCATTTATTATTACATAATAATACTATTTTTTATTTATTCATAACTAATTATATATATATATTTGTTGATA
ATTTTAATACATATTATAAC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain-containing protein

Products: CO2; NADH

Alternate protein names: NA

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MNPKLIVLDPSKCTNCDDCVNKCKEIHGISRIKKANNVPVFCMQCENAPCYNICPVDAIYLKDGIPLVKKERCIGCGMCE
IVCPVGSIFIDKLYAHKCSLCLDTDRITPACLEACKDKALKLVCDECIDSIKEERRKKLMKIISDEWREDI

Sequences:

>Translated_151_residues
MNPKLIVLDPSKCTNCDDCVNKCKEIHGISRIKKANNVPVFCMQCENAPCYNICPVDAIYLKDGIPLVKKERCIGCGMCE
IVCPVGSIFIDKLYAHKCSLCLDTDRITPACLEACKDKALKLVCDECIDSIKEERRKKLMKIISDEWREDI
>Mature_151_residues
MNPKLIVLDPSKCTNCDDCVNKCKEIHGISRIKKANNVPVFCMQCENAPCYNICPVDAIYLKDGIPLVKKERCIGCGMCE
IVCPVGSIFIDKLYAHKCSLCLDTDRITPACLEACKDKALKLVCDECIDSIKEERRKKLMKIISDEWREDI

Specific function: Unknown

COG id: COG1142

COG function: function code C; Fe-S-cluster-containing hydrogenase components 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI87082180, Length=152, Percent_Identity=32.2368421052632, Blast_Score=88, Evalue=2e-19,
Organism=Escherichia coli, GI2367245, Length=155, Percent_Identity=36.7741935483871, Blast_Score=84, Evalue=5e-18,
Organism=Escherichia coli, GI87082179, Length=152, Percent_Identity=32.2368421052632, Blast_Score=79, Evalue=9e-17,
Organism=Escherichia coli, GI1788811, Length=168, Percent_Identity=30.3571428571429, Blast_Score=77, Evalue=5e-16,
Organism=Escherichia coli, GI87082114, Length=183, Percent_Identity=25.6830601092896, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1789067, Length=169, Percent_Identity=31.9526627218935, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1789079, Length=188, Percent_Identity=26.063829787234, Blast_Score=64, Evalue=4e-12,
Organism=Escherichia coli, GI2367345, Length=141, Percent_Identity=32.6241134751773, Blast_Score=61, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR000813 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: 1.2.1.2

Molecular weight: Translated: 17048; Mature: 17048

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

11.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
14.6 %Cys+Met (Translated Protein)
11.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
14.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKLIVLDPSKCTNCDDCVNKCKEIHGISRIKKANNVPVFCMQCENAPCYNICPVDAIY
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEE
LKDGIPLVKKERCIGCGMCEIVCPVGSIFIDKLYAHKCSLCLDTDRITPACLEACKDKAL
ECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KLVCDECIDSIKEERRKKLMKIISDEWREDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNPKLIVLDPSKCTNCDDCVNKCKEIHGISRIKKANNVPVFCMQCENAPCYNICPVDAIY
CCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCEEE
LKDGIPLVKKERCIGCGMCEIVCPVGSIFIDKLYAHKCSLCLDTDRITPACLEACKDKAL
ECCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
KLVCDECIDSIKEERRKKLMKIISDEWREDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]