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The map label for this gene is rpoN [H]

Identifier: 150009754

GI number: 150009754

Start: 3834117

End: 3835574

Strand: Direct

Name: rpoN [H]

Synonym: BDI_3169

Alternate gene names: 150009754

Gene position: 3834117-3835574 (Clockwise)

Preceding gene: 150009753

Following gene: 150009755

Centisome position: 79.69

GC content: 46.57

Gene sequence:

>1458_bases
ATGTTAAAGCAACAGTTACAACAAAAGTTACAGCAAAAGCTATCCCCTCAGCAGATACAGTTGATACGGCTTCTGGAGCT
TCCTGCGATCGAGTTGGAAGAACGTGTGAAGCATGAGCTGGAAGATAACCCAGCCTTGGAGGAAGGAAAAGAGCCTGTTG
ATGATTTTGAACGAACTGAAAGCGAGGAGGGGGGAGAAGAGATCCCTTCTGTTGATACTGAGACCGACCTTTCTTTGGGA
GATTATTTAACGGAAGACGATATACCTGATTATAAGCTACGGGAAATGACCGAGCGTGCGGAACGGAAAGAGGATGTCCC
CTTCTCCGTAAGCCAATCCTTGAATGAGTTTCTGTTACAGCAGCTCGGGTTAAGGGATTTACCGGATAAGCAAATGAAAA
TAGCGGAGTATATCATTGGAAATATCGATGATGACGGTTATTTGAGACGGGACCTTTCGGCGATAGCGGACGATTTGATC
TTCCAAGCCGGACAAGAGGTAGATGAGAAGGAGATCGAGTCTATCTTGAATATCATCCAAGATTTTGAGCCTGCCGGGGT
AGGTGCCCGTGACTTGAAAGAATGTCTGTTGATCCAGCTGGATAAGAAGGAGAATACTCCGGTTACAAATTTGGCGATTC
GTGTTTTGACCGAATATTTCGAGGAGTTCACCCGCAAACATTACGATAAGATCTTACGAGGTCTCGATATCGATGAGGAA
ACGTTAAAGAAGGTCATTCACGAGATTACTATGTTAAATCCGAAACCCGGAAGTAGTTGGGGTGGTTCTATGGAAGTGGC
GATGAGCCAGATCATCCCGGATTTCGTGGTGGAAGCGCATAATGGAGAGCTTATCTTGAGCATGAATAACCGGGGGGTAC
CGGATATGCGTATCAACCGGGAGTATGCCGAGATGTTTCAGGATTATACGGCGAATAAGGCGAACCAAACCGCTGAGAGG
CGGGATGCCGTACAGTTCGTGAAACAAAAGTTGGATTCCGCCCAGTGGTTCATCGACGCTATCAAGCAACGTAATGAGAC
CTTGCAGCGTACCATGGAGGCGATTATCTATTTACAACGGGACTTTTTCCTGACAGGCGATGAGGCTACCCTCCATCCCA
TGATCTTGAAAGACGTAGCGGAGCGTGCGGGATATGATATCTCCACTATTTCCCGGGTAAGTAACAGTAAATATGTGCAA
ACGAATTTCGGTATTTATCCGTTGAAATACTTTTTCTCCGAGTCTATGCAGACCGATACCGGCGAGGAGATCTCGACCCG
TGAGGTGAAAAAGATTATGAAAGAGCATGTAGATGCCGAAGACAAACGAAAACCGTTGACCGATGAGGAGTTGGCTACGA
TCCTGAAAGAAAAAGGATATGTCATCGCCCGGCGTACGGTAGCGAAATACAGGGAGCAACTGGGGATTCCGGTAGCTCGT
CTGAGAAAAGAAATATAA

Upstream 100 bases:

>100_bases
GTTTAATAGCTGTACTGTTAAAACTAAAAACTTATATTTGCATGTTGATAAATAAGAGGGCGGGTGATTGTCCTACCCTT
ACAGTGCAATGATTTAGATT

Downstream 100 bases:

>100_bases
AAGCGTATGAAGCTATTCTCGAATATAATATCCTTCGTGTTCCATCCGTTGCTTATGGTGACCTATGGGATCGTGTTGGC
GTTGATGTTTACATTCCTCG

Product: RNA polymerase sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MLKQQLQQKLQQKLSPQQIQLIRLLELPAIELEERVKHELEDNPALEEGKEPVDDFERTESEEGGEEIPSVDTETDLSLG
DYLTEDDIPDYKLREMTERAERKEDVPFSVSQSLNEFLLQQLGLRDLPDKQMKIAEYIIGNIDDDGYLRRDLSAIADDLI
FQAGQEVDEKEIESILNIIQDFEPAGVGARDLKECLLIQLDKKENTPVTNLAIRVLTEYFEEFTRKHYDKILRGLDIDEE
TLKKVIHEITMLNPKPGSSWGGSMEVAMSQIIPDFVVEAHNGELILSMNNRGVPDMRINREYAEMFQDYTANKANQTAER
RDAVQFVKQKLDSAQWFIDAIKQRNETLQRTMEAIIYLQRDFFLTGDEATLHPMILKDVAERAGYDISTISRVSNSKYVQ
TNFGIYPLKYFFSESMQTDTGEEISTREVKKIMKEHVDAEDKRKPLTDEELATILKEKGYVIARRTVAKYREQLGIPVAR
LRKEI

Sequences:

>Translated_485_residues
MLKQQLQQKLQQKLSPQQIQLIRLLELPAIELEERVKHELEDNPALEEGKEPVDDFERTESEEGGEEIPSVDTETDLSLG
DYLTEDDIPDYKLREMTERAERKEDVPFSVSQSLNEFLLQQLGLRDLPDKQMKIAEYIIGNIDDDGYLRRDLSAIADDLI
FQAGQEVDEKEIESILNIIQDFEPAGVGARDLKECLLIQLDKKENTPVTNLAIRVLTEYFEEFTRKHYDKILRGLDIDEE
TLKKVIHEITMLNPKPGSSWGGSMEVAMSQIIPDFVVEAHNGELILSMNNRGVPDMRINREYAEMFQDYTANKANQTAER
RDAVQFVKQKLDSAQWFIDAIKQRNETLQRTMEAIIYLQRDFFLTGDEATLHPMILKDVAERAGYDISTISRVSNSKYVQ
TNFGIYPLKYFFSESMQTDTGEEISTREVKKIMKEHVDAEDKRKPLTDEELATILKEKGYVIARRTVAKYREQLGIPVAR
LRKEI
>Mature_485_residues
MLKQQLQQKLQQKLSPQQIQLIRLLELPAIELEERVKHELEDNPALEEGKEPVDDFERTESEEGGEEIPSVDTETDLSLG
DYLTEDDIPDYKLREMTERAERKEDVPFSVSQSLNEFLLQQLGLRDLPDKQMKIAEYIIGNIDDDGYLRRDLSAIADDLI
FQAGQEVDEKEIESILNIIQDFEPAGVGARDLKECLLIQLDKKENTPVTNLAIRVLTEYFEEFTRKHYDKILRGLDIDEE
TLKKVIHEITMLNPKPGSSWGGSMEVAMSQIIPDFVVEAHNGELILSMNNRGVPDMRINREYAEMFQDYTANKANQTAER
RDAVQFVKQKLDSAQWFIDAIKQRNETLQRTMEAIIYLQRDFFLTGDEATLHPMILKDVAERAGYDISTISRVSNSKYVQ
TNFGIYPLKYFFSESMQTDTGEEISTREVKKIMKEHVDAEDKRKPLTDEELATILKEKGYVIARRTVAKYREQLGIPVAR
LRKEI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=510, Percent_Identity=33.7254901960784, Blast_Score=273, Evalue=2e-74,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 56032; Mature: 56032

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQQLQQKLQQKLSPQQIQLIRLLELPAIELEERVKHELEDNPALEEGKEPVDDFERTE
CCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHCCCCCHHHHHHCC
SEEGGEEIPSVDTETDLSLGDYLTEDDIPDYKLREMTERAERKEDVPFSVSQSLNEFLLQ
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QLGLRDLPDKQMKIAEYIIGNIDDDGYLRRDLSAIADDLIFQAGQEVDEKEIESILNIIQ
HCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DFEPAGVGARDLKECLLIQLDKKENTPVTNLAIRVLTEYFEEFTRKHYDKILRGLDIDEE
CCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TLKKVIHEITMLNPKPGSSWGGSMEVAMSQIIPDFVVEAHNGELILSMNNRGVPDMRINR
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCH
EYAEMFQDYTANKANQTAERRDAVQFVKQKLDSAQWFIDAIKQRNETLQRTMEAIIYLQR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFFLTGDEATLHPMILKDVAERAGYDISTISRVSNSKYVQTNFGIYPLKYFFSESMQTDT
HEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCC
GEEISTREVKKIMKEHVDAEDKRKPLTDEELATILKEKGYVIARRTVAKYREQLGIPVAR
CCCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHH
LRKEI
HHHCC
>Mature Secondary Structure
MLKQQLQQKLQQKLSPQQIQLIRLLELPAIELEERVKHELEDNPALEEGKEPVDDFERTE
CCHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHCCCCCHHHHHHCC
SEEGGEEIPSVDTETDLSLGDYLTEDDIPDYKLREMTERAERKEDVPFSVSQSLNEFLLQ
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
QLGLRDLPDKQMKIAEYIIGNIDDDGYLRRDLSAIADDLIFQAGQEVDEKEIESILNIIQ
HCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
DFEPAGVGARDLKECLLIQLDKKENTPVTNLAIRVLTEYFEEFTRKHYDKILRGLDIDEE
CCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
TLKKVIHEITMLNPKPGSSWGGSMEVAMSQIIPDFVVEAHNGELILSMNNRGVPDMRINR
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCH
EYAEMFQDYTANKANQTAERRDAVQFVKQKLDSAQWFIDAIKQRNETLQRTMEAIIYLQR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFFLTGDEATLHPMILKDVAERAGYDISTISRVSNSKYVQTNFGIYPLKYFFSESMQTDT
HEEEECCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCC
GEEISTREVKKIMKEHVDAEDKRKPLTDEELATILKEKGYVIARRTVAKYREQLGIPVAR
CCCHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHH
LRKEI
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113171; 10984043 [H]