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The map label for this gene is lacZ [H]

Identifier: 150009705

GI number: 150009705

Start: 3753949

End: 3755781

Strand: Direct

Name: lacZ [H]

Synonym: BDI_3120

Alternate gene names: 150009705

Gene position: 3753949-3755781 (Clockwise)

Preceding gene: 150009704

Following gene: 150009707

Centisome position: 78.02

GC content: 45.17

Gene sequence:

>1833_bases
ATGAAAAAGATTACCGTTGTTTGTTTAGCTCTTTGCTCCTTTCTTTTTGGGGGAGATCTCTTCTCCGTAAAGGCGCAAAT
CCTAGAGAAGTTCACATTGCCTACCCCATGGACAGAGGAGGCATTGAAGGCTGAGATTCCTTTACCGGAATATCCACGTC
CGCAGATGGTGCGTTCGGAATGGTTGAATCTGAATGGTATATGGGATTATATGGGAGGAAAAGACCTGCTTGATCCTGTT
ACGGCAACCACCCCTCCAGTCTTTCCCGCAAAAGTAGAAAAGATACGTGTGCCGTATCCTCCGGAATCGGAATTATCCGG
TATAGCTCGTGGGGGTGATACTTGCCTGTGGTATAAGCGTAGCTTCTCTGTTCCTCAAGCATGGAAGGGCAGGAATGTAT
TGTTGAATTTTGGGGCTGTAGATCGGATCTCGTCTGTGTTTGTCAACGGTAAGAAGGTGGGTACCCATACGGGAGGGTAC
GATGCTTTTAGTTTAAATATTACTGATTATTTGAAATCCGGTGAAAATGTCCTTGTGGTAGGTGCTTATGATCCTAACGA
CGGCCGGGCCGCTTCGGGCAAGAACGGCTCACGTGGAGATTATATTTTTACCTCCGGTATCTGGCAAACCGTTTGGCTTG
AACCGGTAGAGAAACAATATATTTCTCAGATTAAGCTAGTGCCCGATTTAAAAAATAATCGGTTGGAAGTAATTGCTTAT
ACGGAGGATAAGGAGTTAAAGGTAACGGCTATCGCGGATAATGGTACTTCAGAGGTGGCAAGAACCGAAGGAAATTCCAA
TATGAGTTTTTACTTGCCTATCCGGGAACCCCGTCTATGGAGTCCAGATGATCCTTTCTTGTATGGTTTGAAATTGCAGT
TGAAAGATCGAAAGGGTGAGATAATAGATGAGGTATCTTCTTATTTTGGTATGCGTTCTGTTAGTATGGGAAAGGTAGAT
GGTGTGCTTCGGCCTTTATTGAACGGGGAGTTTGTTTTCCATATCGGTCTTTTAGATCAAGGTTATTGGCCCGATGGCGC
ATTTACTGCTCCTACCGACAAGGCTTTGTTGTATGATATAGAACTGGCGAAACGTGCGGGTTTTAATGTAATACGCAAGC
ATATCAAAGTAGAGCCTCAACGTTGGTATTATCATTGTGACAGGCTTGGCTTGATGGTTTGGCAAGATATGCCGAATCTT
TGGGAGCCGGATGGTGAGGATTCCGTGGCGGTTCGTAAACAGTTCCGTGACGAATTGAAAGTGATGATCGACCAACACGT
CAGCTCGCCTTCTATCGTGATGTGGGTCCCGTTTAATGAGAATTGGGGAGCTTTTGAAGCGACAGACATCACAGCTTGGG
TGAAAAAGTACGATCCGAATCGTTGGGTAAACGGTATGTCGGGATATAATTACGCTCCGGGCTATCGTAAGGCTTATGGA
GATCCCAGAAATGGAGATTTCGTGGATATGCATCATTATGGCAAGATCGAGCCTAAGGCGATCCCCCGTCCCGATGATAA
ACGTGCTGCGTCCCTTGGCGAATTCGGAGGAAAAGGTCTATTTGTGCGTGGGCATATGTGGCCTGTACGCAATGACGCTT
ATGAGATGATGTTGAATAGAGAGGTCCTGACCGATACCTATGTATTAATGCTTACCGAGCTAGAGCAGATGATAAACTAT
TTCGGACTAAGCACGGCCATCTATACGCAGACTACAGATGTGGAACATGAGATAAACGGCTTGGTCACTTATGACCGAAA
GGTGGAGAAGATGGATCTGGAGAAAGTTAAGTACATCAATCAGGAAGTTATAAGATGTACAAGAAAAAAGTAG

Upstream 100 bases:

>100_bases
CTCCGTCCGCAAGATAAGCCGGTCTTGATCGATAAGTTGGAGAAAGAAGATCAAGATTTGATCCGGAAATATTATCCGGA
ATTACTTAAATAGTTGGTTT

Downstream 100 bases:

>100_bases
CATATCCTCTAAAATGTTGATAATTAAAGAATACGGGATTCATTGATGAGTTCCGTGTTTTTTTATTTTGTAAGTAAAGA
GCTAATATTTTTCCCTTTTG

Product: beta-glycosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MKKITVVCLALCSFLFGGDLFSVKAQILEKFTLPTPWTEEALKAEIPLPEYPRPQMVRSEWLNLNGIWDYMGGKDLLDPV
TATTPPVFPAKVEKIRVPYPPESELSGIARGGDTCLWYKRSFSVPQAWKGRNVLLNFGAVDRISSVFVNGKKVGTHTGGY
DAFSLNITDYLKSGENVLVVGAYDPNDGRAASGKNGSRGDYIFTSGIWQTVWLEPVEKQYISQIKLVPDLKNNRLEVIAY
TEDKELKVTAIADNGTSEVARTEGNSNMSFYLPIREPRLWSPDDPFLYGLKLQLKDRKGEIIDEVSSYFGMRSVSMGKVD
GVLRPLLNGEFVFHIGLLDQGYWPDGAFTAPTDKALLYDIELAKRAGFNVIRKHIKVEPQRWYYHCDRLGLMVWQDMPNL
WEPDGEDSVAVRKQFRDELKVMIDQHVSSPSIVMWVPFNENWGAFEATDITAWVKKYDPNRWVNGMSGYNYAPGYRKAYG
DPRNGDFVDMHHYGKIEPKAIPRPDDKRAASLGEFGGKGLFVRGHMWPVRNDAYEMMLNREVLTDTYVLMLTELEQMINY
FGLSTAIYTQTTDVEHEINGLVTYDRKVEKMDLEKVKYINQEVIRCTRKK

Sequences:

>Translated_610_residues
MKKITVVCLALCSFLFGGDLFSVKAQILEKFTLPTPWTEEALKAEIPLPEYPRPQMVRSEWLNLNGIWDYMGGKDLLDPV
TATTPPVFPAKVEKIRVPYPPESELSGIARGGDTCLWYKRSFSVPQAWKGRNVLLNFGAVDRISSVFVNGKKVGTHTGGY
DAFSLNITDYLKSGENVLVVGAYDPNDGRAASGKNGSRGDYIFTSGIWQTVWLEPVEKQYISQIKLVPDLKNNRLEVIAY
TEDKELKVTAIADNGTSEVARTEGNSNMSFYLPIREPRLWSPDDPFLYGLKLQLKDRKGEIIDEVSSYFGMRSVSMGKVD
GVLRPLLNGEFVFHIGLLDQGYWPDGAFTAPTDKALLYDIELAKRAGFNVIRKHIKVEPQRWYYHCDRLGLMVWQDMPNL
WEPDGEDSVAVRKQFRDELKVMIDQHVSSPSIVMWVPFNENWGAFEATDITAWVKKYDPNRWVNGMSGYNYAPGYRKAYG
DPRNGDFVDMHHYGKIEPKAIPRPDDKRAASLGEFGGKGLFVRGHMWPVRNDAYEMMLNREVLTDTYVLMLTELEQMINY
FGLSTAIYTQTTDVEHEINGLVTYDRKVEKMDLEKVKYINQEVIRCTRKK
>Mature_610_residues
MKKITVVCLALCSFLFGGDLFSVKAQILEKFTLPTPWTEEALKAEIPLPEYPRPQMVRSEWLNLNGIWDYMGGKDLLDPV
TATTPPVFPAKVEKIRVPYPPESELSGIARGGDTCLWYKRSFSVPQAWKGRNVLLNFGAVDRISSVFVNGKKVGTHTGGY
DAFSLNITDYLKSGENVLVVGAYDPNDGRAASGKNGSRGDYIFTSGIWQTVWLEPVEKQYISQIKLVPDLKNNRLEVIAY
TEDKELKVTAIADNGTSEVARTEGNSNMSFYLPIREPRLWSPDDPFLYGLKLQLKDRKGEIIDEVSSYFGMRSVSMGKVD
GVLRPLLNGEFVFHIGLLDQGYWPDGAFTAPTDKALLYDIELAKRAGFNVIRKHIKVEPQRWYYHCDRLGLMVWQDMPNL
WEPDGEDSVAVRKQFRDELKVMIDQHVSSPSIVMWVPFNENWGAFEATDITAWVKKYDPNRWVNGMSGYNYAPGYRKAYG
DPRNGDFVDMHHYGKIEPKAIPRPDDKRAASLGEFGGKGLFVRGHMWPVRNDAYEMMLNREVLTDTYVLMLTELEQMINY
FGLSTAIYTQTTDVEHEINGLVTYDRKVEKMDLEKVKYINQEVIRCTRKK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI48994920, Length=396, Percent_Identity=22.7272727272727, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787903, Length=215, Percent_Identity=25.1162790697674, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24655438, Length=433, Percent_Identity=25.1732101616628, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI62471735, Length=433, Percent_Identity=25.1732101616628, Blast_Score=87, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922582, Length=433, Percent_Identity=25.1732101616628, Blast_Score=86, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR011013
- InterPro:   IPR014718
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR004199
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02929 Bgal_small_N; PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 69501; Mature: 69501

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKITVVCLALCSFLFGGDLFSVKAQILEKFTLPTPWTEEALKAEIPLPEYPRPQMVRSE
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCHHHHHHH
WLNLNGIWDYMGGKDLLDPVTATTPPVFPAKVEKIRVPYPPESELSGIARGGDTCLWYKR
CCCCCCEEECCCCCHHHCCCCCCCCCCCCCCHHEEECCCCCCHHHHHHHCCCCEEEEEEE
SFSVPQAWKGRNVLLNFGAVDRISSVFVNGKKVGTHTGGYDAFSLNITDYLKSGENVLVV
CCCCCCCCCCCEEEEECCCHHHHHHHHCCCEEECCCCCCCEEEEEEHHHHHHCCCCEEEE
GAYDPNDGRAASGKNGSRGDYIFTSGIWQTVWLEPVEKQYISQIKLVPDLKNNRLEVIAY
EEECCCCCCCCCCCCCCCCCEEEECCCEEEECCCHHHHHHHHHEEECCCCCCCCEEEEEE
TEDKELKVTAIADNGTSEVARTEGNSNMSFYLPIREPRLWSPDDPFLYGLKLQLKDRKGE
ECCCCEEEEEEECCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCH
IIDEVSSYFGMRSVSMGKVDGVLRPLLNGEFVFHIGLLDQGYWPDGAFTAPTDKALLYDI
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEE
ELAKRAGFNVIRKHIKVEPQRWYYHCDRLGLMVWQDMPNLWEPDGEDSVAVRKQFRDELK
HHHHHCCHHHHHHHHCCCCHHHEEEHHHHCEEHHHCCCCCCCCCCCCHHHHHHHHHHHHH
VMIDQHVSSPSIVMWVPFNENWGAFEATDITAWVKKYDPNRWVNGMSGYNYAPGYRKAYG
HHHHHCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHCC
DPRNGDFVDMHHYGKIEPKAIPRPDDKRAASLGEFGGKGLFVRGHMWPVRNDAYEMMLNR
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
EVLTDTYVLMLTELEQMINYFGLSTAIYTQTTDVEHEINGLVTYDRKVEKMDLEKVKYIN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCEEEECCHHHHHHHHHHHHHH
QEVIRCTRKK
HHHHHHHCCC
>Mature Secondary Structure
MKKITVVCLALCSFLFGGDLFSVKAQILEKFTLPTPWTEEALKAEIPLPEYPRPQMVRSE
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHEECCCCCCCCCHHHHHHH
WLNLNGIWDYMGGKDLLDPVTATTPPVFPAKVEKIRVPYPPESELSGIARGGDTCLWYKR
CCCCCCEEECCCCCHHHCCCCCCCCCCCCCCHHEEECCCCCCHHHHHHHCCCCEEEEEEE
SFSVPQAWKGRNVLLNFGAVDRISSVFVNGKKVGTHTGGYDAFSLNITDYLKSGENVLVV
CCCCCCCCCCCEEEEECCCHHHHHHHHCCCEEECCCCCCCEEEEEEHHHHHHCCCCEEEE
GAYDPNDGRAASGKNGSRGDYIFTSGIWQTVWLEPVEKQYISQIKLVPDLKNNRLEVIAY
EEECCCCCCCCCCCCCCCCCEEEECCCEEEECCCHHHHHHHHHEEECCCCCCCCEEEEEE
TEDKELKVTAIADNGTSEVARTEGNSNMSFYLPIREPRLWSPDDPFLYGLKLQLKDRKGE
ECCCCEEEEEEECCCCCHHHCCCCCCCEEEEEEECCCCCCCCCCCEEEEEEEEEECCCCH
IIDEVSSYFGMRSVSMGKVDGVLRPLLNGEFVFHIGLLDQGYWPDGAFTAPTDKALLYDI
HHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCCCEEEEEE
ELAKRAGFNVIRKHIKVEPQRWYYHCDRLGLMVWQDMPNLWEPDGEDSVAVRKQFRDELK
HHHHHCCHHHHHHHHCCCCHHHEEEHHHHCEEHHHCCCCCCCCCCCCHHHHHHHHHHHHH
VMIDQHVSSPSIVMWVPFNENWGAFEATDITAWVKKYDPNRWVNGMSGYNYAPGYRKAYG
HHHHHCCCCCCEEEEEEECCCCCCEEHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHHHCC
DPRNGDFVDMHHYGKIEPKAIPRPDDKRAASLGEFGGKGLFVRGHMWPVRNDAYEMMLNR
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHH
EVLTDTYVLMLTELEQMINYFGLSTAIYTQTTDVEHEINGLVTYDRKVEKMDLEKVKYIN
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHCCCEEEECCHHHHHHHHHHHHHH
QEVIRCTRKK
HHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8088532; 10360571; 9741105 [H]