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The map label for this gene is gacS [H]

Identifier: 150008173

GI number: 150008173

Start: 1803047

End: 1804615

Strand: Reverse

Name: gacS [H]

Synonym: BDI_1539

Alternate gene names: 150008173

Gene position: 1804615-1803047 (Counterclockwise)

Preceding gene: 150008174

Following gene: 150008172

Centisome position: 37.51

GC content: 40.03

Gene sequence:

>1569_bases
ATGGGACAATGTAATAAAATGCTAGAGGAAATGAGCTTTAATGCCCTATTCCAGAAATTACCTGTTGGAATTGAAGTTTA
TGACGAGAGGGGGCAGCTAGTGGATCTCAATAGGACGGACGAGAATTTGTTCGCTATAAGCCGGAAGGATATTATGGGAA
TAAATCTTTTCGAGAATCCTAATTTTCCAGAGGACAAGATCGCTCGTCTGAAACAGGGAGAAATGGTTGTGTTCGATTGT
AATTATGAATTTGATAATATACAAACGAATGAGTACTATAACATAGACCCAAAAAAGGAAGGTATAAATTTACGTCTGTC
TATTAAATGTGTGCCGTTGAAAGGAGAGGCTGAAGATATCTGTGGATATGTACTAATGTTTACGGATGAGACGGAAGAAT
ATCAAAAGTCGGAGAAAGTAGAGGAACTGTTCACGAAGTTGAAAACGGTTATGCGTTGCAGTGATTCCTTGCTGTGGGAA
TATGATGTTAAAACCGATAAGATGCATATCGATCTGGATTTACGTGTTTTAGGTAATAAATCCAGATTGAGAGCAAGTAA
CTTGACAACGAAAAAAGATTTTTGTGAGATCGTACATCCGGAAGATCGGGAAAGGGTACTTGTACAAGGCTTTGAAAAGA
TTGTTACGGGTGAGATCGGTGAATATAGTATTCAATACCGACAGCTTTTTGAGGGGCAATATGTTTGGGTGAGAGCGTAT
GCGTACCCTTATAAATATGATGAGGCGGGAAGACCAGCGAAAATCCTTTATTACTTGATGGACATAACGGATGAGATCGC
CATGCAAGATAAATTGCGCTGGGTGGAACGAGAGCGTCAACGGAAAGAATATGAGTTAGCTAAGGCTAAAGAGGCGAATC
GGCTTAAGTCTATGTTCTTGGCGAATATGAGTCATGAGATCCGTACGCCTTTAAATGCTATTGTTGGTTTTGCGAATATA
CTGGCAGAGACCAAGGAGATAGAGGAGGAGGAACGAGAGTTTTATGTGGATATCATTGACAAGAATAGTGAATTGCTTCT
GCAATTGATTGAGGATATCCTTGATTTCTCAAAGATTGAGGCGGGAACCTTTGACATTACGAAGAAAAATTTTGATTTTA
AGGAGATCTGTGAGGAAACTTATGCGGTACATGCCTTGAAGATTCCGAAAAACGTTCGATTTGTTTTCGACCGGGGCTCA
CAACCCGTGACCGTTTTCTCAGATCCGAAACGAGTCATACAGGTTATTTCTAATTTCTTGACAAATGCGATTAAGTTTAC
TTCGGAAGGTGAGATCAAATTAGCCTATAAGGTATCGAATGATGAAATACGAGTTTCGGTCTCGGATACGGGAATGGGAA
TCTCGGATGACGATTGTAGAACTATATTCGATCGCTTTGTTAAGTTGAACGCTTGCAAACAAGGTACCGGATTAGGATTG
GCGATTTGCAAAAATATTATCGAGAAACTAGGGGGACGAATCGGGGTAGAATCTACATTGGGAGTAGGTTCCACTTTTTG
GTTTACCTTGCCTTTGAAGGAGATTAAAGAGAATGAACCTAAGTTCTAA

Upstream 100 bases:

>100_bases
TGCTGCAATCGTTATTATCGTTCATTTGTTGTATAAATAACAGAGGAGATTGCTTGATTGATTTAAAGTAGGGAATTAGT
GGTTATAATTTAATATGACG

Downstream 100 bases:

>100_bases
GGGAATATATATTATTTTACGGATATGTTTTAAAGCATGGTTTTGTTAAATCTGGTTAAGGAAATGAGATTTGCAAATAA
AATATTATGCTACATAACAT

Product: putative two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 522; Mature: 521

Protein sequence:

>522_residues
MGQCNKMLEEMSFNALFQKLPVGIEVYDERGQLVDLNRTDENLFAISRKDIMGINLFENPNFPEDKIARLKQGEMVVFDC
NYEFDNIQTNEYYNIDPKKEGINLRLSIKCVPLKGEAEDICGYVLMFTDETEEYQKSEKVEELFTKLKTVMRCSDSLLWE
YDVKTDKMHIDLDLRVLGNKSRLRASNLTTKKDFCEIVHPEDRERVLVQGFEKIVTGEIGEYSIQYRQLFEGQYVWVRAY
AYPYKYDEAGRPAKILYYLMDITDEIAMQDKLRWVERERQRKEYELAKAKEANRLKSMFLANMSHEIRTPLNAIVGFANI
LAETKEIEEEEREFYVDIIDKNSELLLQLIEDILDFSKIEAGTFDITKKNFDFKEICEETYAVHALKIPKNVRFVFDRGS
QPVTVFSDPKRVIQVISNFLTNAIKFTSEGEIKLAYKVSNDEIRVSVSDTGMGISDDDCRTIFDRFVKLNACKQGTGLGL
AICKNIIEKLGGRIGVESTLGVGSTFWFTLPLKEIKENEPKF

Sequences:

>Translated_522_residues
MGQCNKMLEEMSFNALFQKLPVGIEVYDERGQLVDLNRTDENLFAISRKDIMGINLFENPNFPEDKIARLKQGEMVVFDC
NYEFDNIQTNEYYNIDPKKEGINLRLSIKCVPLKGEAEDICGYVLMFTDETEEYQKSEKVEELFTKLKTVMRCSDSLLWE
YDVKTDKMHIDLDLRVLGNKSRLRASNLTTKKDFCEIVHPEDRERVLVQGFEKIVTGEIGEYSIQYRQLFEGQYVWVRAY
AYPYKYDEAGRPAKILYYLMDITDEIAMQDKLRWVERERQRKEYELAKAKEANRLKSMFLANMSHEIRTPLNAIVGFANI
LAETKEIEEEEREFYVDIIDKNSELLLQLIEDILDFSKIEAGTFDITKKNFDFKEICEETYAVHALKIPKNVRFVFDRGS
QPVTVFSDPKRVIQVISNFLTNAIKFTSEGEIKLAYKVSNDEIRVSVSDTGMGISDDDCRTIFDRFVKLNACKQGTGLGL
AICKNIIEKLGGRIGVESTLGVGSTFWFTLPLKEIKENEPKF
>Mature_521_residues
GQCNKMLEEMSFNALFQKLPVGIEVYDERGQLVDLNRTDENLFAISRKDIMGINLFENPNFPEDKIARLKQGEMVVFDCN
YEFDNIQTNEYYNIDPKKEGINLRLSIKCVPLKGEAEDICGYVLMFTDETEEYQKSEKVEELFTKLKTVMRCSDSLLWEY
DVKTDKMHIDLDLRVLGNKSRLRASNLTTKKDFCEIVHPEDRERVLVQGFEKIVTGEIGEYSIQYRQLFEGQYVWVRAYA
YPYKYDEAGRPAKILYYLMDITDEIAMQDKLRWVERERQRKEYELAKAKEANRLKSMFLANMSHEIRTPLNAIVGFANIL
AETKEIEEEEREFYVDIIDKNSELLLQLIEDILDFSKIEAGTFDITKKNFDFKEICEETYAVHALKIPKNVRFVFDRGSQ
PVTVFSDPKRVIQVISNFLTNAIKFTSEGEIKLAYKVSNDEIRVSVSDTGMGISDDDCRTIFDRFVKLNACKQGTGLGLA
ICKNIIEKLGGRIGVESTLGVGSTFWFTLPLKEIKENEPKF

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693157, Length=264, Percent_Identity=38.6363636363636, Blast_Score=159, Evalue=3e-40,
Organism=Escherichia coli, GI1789149, Length=255, Percent_Identity=37.6470588235294, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87081816, Length=264, Percent_Identity=34.8484848484849, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788713, Length=240, Percent_Identity=34.1666666666667, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI48994928, Length=350, Percent_Identity=28.2857142857143, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI1788393, Length=281, Percent_Identity=28.8256227758007, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1790436, Length=252, Percent_Identity=29.3650793650794, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786912, Length=237, Percent_Identity=28.6919831223629, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1786600, Length=227, Percent_Identity=28.6343612334802, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788549, Length=237, Percent_Identity=28.6919831223629, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082128, Length=315, Percent_Identity=22.8571428571429, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786783, Length=232, Percent_Identity=28.0172413793103, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790346, Length=280, Percent_Identity=26.7857142857143, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790861, Length=220, Percent_Identity=27.7272727272727, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1790300, Length=262, Percent_Identity=24.8091603053435, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 60534; Mature: 60403

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS50112 PAS ; PS50113 PAC ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGQCNKMLEEMSFNALFQKLPVGIEVYDERGQLVDLNRTDENLFAISRKDIMGINLFENP
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEEEECCCEEEEEECCCC
NFPEDKIARLKQGEMVVFDCNYEFDNIQTNEYYNIDPKKEGINLRLSIKCVPLKGEAEDI
CCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCEEEEEEEEEECCCCHHHC
CGYVLMFTDETEEYQKSEKVEELFTKLKTVMRCSDSLLWEYDVKTDKMHIDLDLRVLGNK
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEEEECCC
SRLRASNLTTKKDFCEIVHPEDRERVLVQGFEKIVTGEIGEYSIQYRQLFEGQYVWVRAY
CCEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEE
AYPYKYDEAGRPAKILYYLMDITDEIAMQDKLRWVERERQRKEYELAKAKEANRLKSMFL
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANMSHEIRTPLNAIVGFANILAETKEIEEEEREFYVDIIDKNSELLLQLIEDILDFSKIE
HHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHCC
AGTFDITKKNFDFKEICEETYAVHALKIPKNVRFVFDRGSQPVTVFSDPKRVIQVISNFL
CCEEEEECCCCCHHHHHHHHHHEEEEECCCCEEEEEECCCCCEEEECCHHHHHHHHHHHH
TNAIKFTSEGEIKLAYKVSNDEIRVSVSDTGMGISDDDCRTIFDRFVKLNACKQGTGLGL
HHHHEECCCCCEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
AICKNIIEKLGGRIGVESTLGVGSTFWFTLPLKEIKENEPKF
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCC
>Mature Secondary Structure 
GQCNKMLEEMSFNALFQKLPVGIEVYDERGQLVDLNRTDENLFAISRKDIMGINLFENP
CHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCCEEEEECCCEEEEEECCCC
NFPEDKIARLKQGEMVVFDCNYEFDNIQTNEYYNIDPKKEGINLRLSIKCVPLKGEAEDI
CCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCCCCEEEEEEEEEECCCCHHHC
CGYVLMFTDETEEYQKSEKVEELFTKLKTVMRCSDSLLWEYDVKTDKMHIDLDLRVLGNK
CEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEEEEEECCC
SRLRASNLTTKKDFCEIVHPEDRERVLVQGFEKIVTGEIGEYSIQYRQLFEGQYVWVRAY
CCEECCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCEEEEEEE
AYPYKYDEAGRPAKILYYLMDITDEIAMQDKLRWVERERQRKEYELAKAKEANRLKSMFL
ECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ANMSHEIRTPLNAIVGFANILAETKEIEEEEREFYVDIIDKNSELLLQLIEDILDFSKIE
HHCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHHHHCC
AGTFDITKKNFDFKEICEETYAVHALKIPKNVRFVFDRGSQPVTVFSDPKRVIQVISNFL
CCEEEEECCCCCHHHHHHHHHHEEEEECCCCEEEEEECCCCCEEEECCHHHHHHHHHHHH
TNAIKFTSEGEIKLAYKVSNDEIRVSVSDTGMGISDDDCRTIFDRFVKLNACKQGTGLGL
HHHHEECCCCCEEEEEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
AICKNIIEKLGGRIGVESTLGVGSTFWFTLPLKEIKENEPKF
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]