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The map label for this gene is oprM [H]

Identifier: 150006273

GI number: 150006273

Start: 4673763

End: 4675145

Strand: Direct

Name: oprM [H]

Synonym: BVU_3784

Alternate gene names: 150006273

Gene position: 4673763-4675145 (Clockwise)

Preceding gene: 150006272

Following gene: 150006275

Centisome position: 90.52

GC content: 42.3

Gene sequence:

>1383_bases
ATGAAAGAAATGATGAAACATACATTATTATATATAGCCGTATCAGGAATGACTTTGCTGACTTCTTGCCAATTAGGTAA
GCACTACACACGTCCCGACTTACATCTTCCCCAACAATTAGATACATTACGACAACAGGATACATTATCTATAGCCGATA
TGCAATGGTGGGAAATCTATACGGACACAACCTTGCAAAGCCTGATAGAAAAGACGTTGGATAACAACAAAGACATGAAA
ATAGCTGCGGCACGTGTAAAAGAACTTGCCGCAATGAAGCGCATAGACTTTGCCAACCTTTTCCCCCAACTGAACGGAAG
CGCTTATGCCCAAAAAGAAGGAAGTAATTATGGTGGTGACAACTATAAAAATGACCCCGAACAGGGTGGTAAACTTACCG
TGACTTGGGAATTAGACTTATGGGGTAACCTGCGTTGGGCAAAAGACAAAAGCATAGCACAATTTCTGGGAAGCATCGAG
GCGCAACGTGCTTTGAAAATGAGTATTGTGTCCGAAGTGGCACAAGCCTATTTTGAATTGGTCGCATTAGACAATGAGCT
GAACATCGTTCGTCAAACACTCTATGCCCGTAAAGAAGGAGTACGCCTGGCAAAGCTTCGTTTTGAAGGAGGACTAACTT
CAGAAACTTCCTATCAACAGGCACAAGTGGAGTATGCACGTACCGCAACTTTAGTTCCTGAACTGGAAAGGAAAATTTCA
TTGAAAGAAAATGATATCGCATTCCTGGCAGGCGAATACCCGCACCGCATACAGCGTTCCATTCTACCCGAAGAGGTAAA
ACTACCTGAAACATTACCAGTAGGTCTACCCTCCACTCTGTTGGAACGTCGTCCCGATGTACGCGAAGCAGAACAGAAAT
TAATAGCAGCCAATGCCAGTGTAGGTATTGCCTATACCAATATATTCCCACGACTCAGCCTCACAGCACAATATGGCGTA
GAAAGTGAAGAATTCTCTGATTTCTTTAAATCACCCATTCATTATATCAGTGGCAATCTTCTCACTCCTCTCTTCGCCAT
GGGAAAATACAGAGCAACCCTAAAGGCTAAGAAAGCCGCATACGAACAGGAATGCTACAGCTATGAAAAATCAGTGCTGA
CTGCATTCAAAGAGGCCCGCAATGCCATTGTGGACTTCAATAAGATAAAAGATATCTACGAATCGCGTCTGCAACTGGAA
CGTTCCTCTAAAGCAACAATGGAACTGGCCCAATTGCAATACATCAATGGAGTAATAGGCTATTTGGATGTATTGGATGC
ACAACGTAGCTATTTCGATGCACAAATAGGATTAAGTAATGCAATAAGAGACAAACAAATTACATTAGTAAAATTGTATA
AAGCATTAGGGGGTGGATGGTAA

Upstream 100 bases:

>100_bases
GATCTTTGCTGTAACTGTGGGAATTTTGTTAGTACCTTTCTTCTTTGTACTGATATACAAGATGAAAGCGAAGATGAAAC
AAAAATAAGGATATAAAAAG

Downstream 100 bases:

>100_bases
CCATCCCCTCTTAATTTGATTTTCCTTTATCCGGAAAATCAAATTAAGATATATGCCTGATAAGACGTATTGTTCTCTAC
CGGTGTAAGTGACAAATAAC

Product: outer membrane protein oprM precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MKEMMKHTLLYIAVSGMTLLTSCQLGKHYTRPDLHLPQQLDTLRQQDTLSIADMQWWEIYTDTTLQSLIEKTLDNNKDMK
IAAARVKELAAMKRIDFANLFPQLNGSAYAQKEGSNYGGDNYKNDPEQGGKLTVTWELDLWGNLRWAKDKSIAQFLGSIE
AQRALKMSIVSEVAQAYFELVALDNELNIVRQTLYARKEGVRLAKLRFEGGLTSETSYQQAQVEYARTATLVPELERKIS
LKENDIAFLAGEYPHRIQRSILPEEVKLPETLPVGLPSTLLERRPDVREAEQKLIAANASVGIAYTNIFPRLSLTAQYGV
ESEEFSDFFKSPIHYISGNLLTPLFAMGKYRATLKAKKAAYEQECYSYEKSVLTAFKEARNAIVDFNKIKDIYESRLQLE
RSSKATMELAQLQYINGVIGYLDVLDAQRSYFDAQIGLSNAIRDKQITLVKLYKALGGGW

Sequences:

>Translated_460_residues
MKEMMKHTLLYIAVSGMTLLTSCQLGKHYTRPDLHLPQQLDTLRQQDTLSIADMQWWEIYTDTTLQSLIEKTLDNNKDMK
IAAARVKELAAMKRIDFANLFPQLNGSAYAQKEGSNYGGDNYKNDPEQGGKLTVTWELDLWGNLRWAKDKSIAQFLGSIE
AQRALKMSIVSEVAQAYFELVALDNELNIVRQTLYARKEGVRLAKLRFEGGLTSETSYQQAQVEYARTATLVPELERKIS
LKENDIAFLAGEYPHRIQRSILPEEVKLPETLPVGLPSTLLERRPDVREAEQKLIAANASVGIAYTNIFPRLSLTAQYGV
ESEEFSDFFKSPIHYISGNLLTPLFAMGKYRATLKAKKAAYEQECYSYEKSVLTAFKEARNAIVDFNKIKDIYESRLQLE
RSSKATMELAQLQYINGVIGYLDVLDAQRSYFDAQIGLSNAIRDKQITLVKLYKALGGGW
>Mature_460_residues
MKEMMKHTLLYIAVSGMTLLTSCQLGKHYTRPDLHLPQQLDTLRQQDTLSIADMQWWEIYTDTTLQSLIEKTLDNNKDMK
IAAARVKELAAMKRIDFANLFPQLNGSAYAQKEGSNYGGDNYKNDPEQGGKLTVTWELDLWGNLRWAKDKSIAQFLGSIE
AQRALKMSIVSEVAQAYFELVALDNELNIVRQTLYARKEGVRLAKLRFEGGLTSETSYQQAQVEYARTATLVPELERKIS
LKENDIAFLAGEYPHRIQRSILPEEVKLPETLPVGLPSTLLERRPDVREAEQKLIAANASVGIAYTNIFPRLSLTAQYGV
ESEEFSDFFKSPIHYISGNLLTPLFAMGKYRATLKAKKAAYEQECYSYEKSVLTAFKEARNAIVDFNKIKDIYESRLQLE
RSSKATMELAQLQYINGVIGYLDVLDAQRSYFDAQIGLSNAIRDKQITLVKLYKALGGGW

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=450, Percent_Identity=30, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI2367348, Length=489, Percent_Identity=24.5398773006135, Blast_Score=103, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 52164; Mature: 52164

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEMMKHTLLYIAVSGMTLLTSCQLGKHYTRPDLHLPQQLDTLRQQDTLSIADMQWWEIY
CHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEECCCHHHHHH
TDTTLQSLIEKTLDNNKDMKIAAARVKELAAMKRIDFANLFPQLNGSAYAQKEGSNYGGD
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCC
NYKNDPEQGGKLTVTWELDLWGNLRWAKDKSIAQFLGSIEAQRALKMSIVSEVAQAYFEL
CCCCCCCCCCEEEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALDNELNIVRQTLYARKEGVRLAKLRFEGGLTSETSYQQAQVEYARTATLVPELERKIS
HHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
LKENDIAFLAGEYPHRIQRSILPEEVKLPETLPVGLPSTLLERRPDVREAEQKLIAANAS
CCCCCEEEEECCCHHHHHHHCCCHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCC
VGIAYTNIFPRLSLTAQYGVESEEFSDFFKSPIHYISGNLLTPLFAMGKYRATLKAKKAA
CEEEEECCCCCEEEHHHCCCCHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YEQECYSYEKSVLTAFKEARNAIVDFNKIKDIYESRLQLERSSKATMELAQLQYINGVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLDVLDAQRSYFDAQIGLSNAIRDKQITLVKLYKALGGGW
HHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKEMMKHTLLYIAVSGMTLLTSCQLGKHYTRPDLHLPQQLDTLRQQDTLSIADMQWWEIY
CHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCEECCCHHHHHH
TDTTLQSLIEKTLDNNKDMKIAAARVKELAAMKRIDFANLFPQLNGSAYAQKEGSNYGGD
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCC
NYKNDPEQGGKLTVTWELDLWGNLRWAKDKSIAQFLGSIEAQRALKMSIVSEVAQAYFEL
CCCCCCCCCCEEEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VALDNELNIVRQTLYARKEGVRLAKLRFEGGLTSETSYQQAQVEYARTATLVPELERKIS
HHCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
LKENDIAFLAGEYPHRIQRSILPEEVKLPETLPVGLPSTLLERRPDVREAEQKLIAANAS
CCCCCEEEEECCCHHHHHHHCCCHHCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHCCCC
VGIAYTNIFPRLSLTAQYGVESEEFSDFFKSPIHYISGNLLTPLFAMGKYRATLKAKKAA
CEEEEECCCCCEEEHHHCCCCHHHHHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
YEQECYSYEKSVLTAFKEARNAIVDFNKIKDIYESRLQLERSSKATMELAQLQYINGVIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YLDVLDAQRSYFDAQIGLSNAIRDKQITLVKLYKALGGGW
HHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]