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The map label for this gene is mdtB [H]

Identifier: 150006088

GI number: 150006088

Start: 4461975

End: 4464269

Strand: Direct

Name: mdtB [H]

Synonym: BVU_3597

Alternate gene names: 150006088

Gene position: 4461975-4464269 (Clockwise)

Preceding gene: 150006087

Following gene: 150006089

Centisome position: 86.42

GC content: 45.1

Gene sequence:

>2295_bases
ATGAAACTCGTAAAATATTTTCTACAGAAGAAAGCAGTCACTATCTTGCTGTTGGTATTGATATTGGCCGGTGGGCTGTT
CTCCTATATCAAAATGGGAAAACTGGAAGACGCCCCCTTTACCATCAAACAAGCACTCGTACTGACTCCGTATCCCGGAG
CATCCCCCTCCGAGGTACAATCTCAAGTAACAGATGTTCTGGAAGAATCCATCCAATCTCTTGGTGAACTTTATTATCTG
AAAACAGAAAACCGAACAGGACTTTCAAAAATAACCGTTTATGTAAAAAAGGAAATCCGGGCAGACGAAATGCAACAACT
TTGGGACAAATTACGCCGTAAAGTAAATGATGTACAAAGCAAACTTCCCGCCGGAGCAGGTCCATCTGTTGTTAACGATG
ATTTTGGAGATGTATTGGGAGTTTTCTATGGACTGACTGGAAAGAGCCATACCTATCGGGAACTGGAAGACGAAGCCAAG
ATTATCAAAAACGAACTATTGAAAATAAAAGATGTTGCTAAAATAGAAATCTACGGAATACAGACACCTACCATTGACGT
GTCTGTCAGCCCATCCGTCATGGCACAAAGCGGAATTACGACCTCGGATATAGCCCGCGCTTTCGAAGCACAAAATAAAG
TGGTGGATGCCGGAGGTATTGATGCCGGACAAAATCGGCTGCGCATTGAATCCACTGGAAATTTTTATTCATTAGATGAT
ATAAGAAACCTGACTATCGTATCACGCTCCGGTGAACATTTCCGCCTTGCCGACATTGCACAAATAGAAGAAAGTTATCA
GACACCTGCCAGCAATCTGATGCGCATTGACGGTAATCCGGCCATAGGTATTGCCATCTCCACCGTCCCCACCGGAAATG
TAGTGGATATGGCAGAAGCTGTGAAAAAACGCATTGACGAATTATCCCAATCCATGCCTGAAGGATATGAACTGACCTCT
ATCTACGATCAAGGATATGAATCAGCCGTTGCCAATCAAGGCTTTGTCTTGAATCTCATCATTTCCGTTTTAACTGTTAT
AGCCATTCTATTATTTTTCATCGGGTTCAAGAATGGCACGCTGATAGGTAGCGGACTGATTTTTTCCATCTTCGCCACTC
TCATTGTCATGATGGCTTGCGGCATCGCACTCCAGCGAATGTCACTGGCCGCCATCATTATCGCCATGGGGATGTTGGTA
GACAATGCCATTGTTGTGTCAGACTCCGCTTTAATCAATATGGAGCGTGGAATGCGGAAACGTGTAGCCATCATGCAGGC
TTGCTCGACTACCGCCCTGCCCTTGTTGGCAGCAACCGTAATCGCTATTCTTACTTTCCTTCCTATCTATTATTCACCAC
ATATCACAGGTGAACTGTTATCCTCATTAGTCGTGGTTATCGGAGTATCCCTGATGTTCAGCTGGGTGTTCGCACTGACA
CAGACACCTTTCTTTATACAGGAATTTGTCCGCCGCCCACGTCCCGAGGAACTGAAAGCGGCACTGTTTGACGGAAAATA
TTACAACCGTTTCCGCAATGCTTTACATTGGGTGCTCCGCCACCGGTCGGTCACCATCGGCTCACTGGCCATTATGCTGA
TACTCTCTGCCTGGAGTTTCAAATTTATTCCTAAAGTATTCGTACCCGCACTGGAAAAACAATACTTTACCGTAGATATG
TGGCTGCCCGAAGGAACAAACATTAACGAAACAGACCGCATGGCAAGCAGTCTGGCAGACTATATACGCGGACACGAAGA
AACAGAAATGGTTTCTACCTACATAGGTCGCACGCCCCCCCGCTATTATTTATCCAATGTATCTTTCGGTCCTCAATCCA
ATTATGCACAAATACTGGTAAAATGCAAGACCTCCAAAGATTCAAAGGAATTACATGCGCTACTGCAAGACTCCATTCGC
CAGAAATATCCCGAACCCCTGATAAAAGTGAACAAATTCGAGCTTAGCCCTTTGACAGAAGCCGTCATCGAAGCCCGTTT
TCTAGGACCGGATCCTGCTGTACTAGATTCACTGGCAGGGAAAGCAATAGAAATCATGCGCCGCAATCCCAAAGTAGCGG
ACGCCCGCAACGAATGGGGCAACATGAGTTTAATGATCCGCCCTGTTTACGATCCGGTAAAAGCCGGAGCACTGGGTATC
ACCAAAACACAAATGATGCAATCCGTCAAGTCTATCAGTGATGGAACTCCTGTAGGAATTTATCGGGATAATGAAAAGAA
AGTACCTGTATTACTAAAATCGGAAGGGGTCATATTACAGACGAACGATCTTTAG

Upstream 100 bases:

>100_bases
TGTATCCTGATGAAACTGTAGCAAACAGTGGGCTCCGCTTCCTATCGGATGGAATGAAAATAAGTGTAGAAAACGAATAA
TATCACAGAAATAAACAATC

Downstream 100 bases:

>100_bases
GGGATTTCTCTGTTTGGAACGGGGAGCATTCGGCCCTCTGTCACAAGTAACGGAAAAAATTGAAACCACTTGGGAATTTC
CGCAAATACGTACTTATAAC

Product: AcrB/D/F family transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug transporter MdtB [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKLVKYFLQKKAVTILLLVLILAGGLFSYIKMGKLEDAPFTIKQALVLTPYPGASPSEVQSQVTDVLEESIQSLGELYYL
KTENRTGLSKITVYVKKEIRADEMQQLWDKLRRKVNDVQSKLPAGAGPSVVNDDFGDVLGVFYGLTGKSHTYRELEDEAK
IIKNELLKIKDVAKIEIYGIQTPTIDVSVSPSVMAQSGITTSDIARAFEAQNKVVDAGGIDAGQNRLRIESTGNFYSLDD
IRNLTIVSRSGEHFRLADIAQIEESYQTPASNLMRIDGNPAIGIAISTVPTGNVVDMAEAVKKRIDELSQSMPEGYELTS
IYDQGYESAVANQGFVLNLIISVLTVIAILLFFIGFKNGTLIGSGLIFSIFATLIVMMACGIALQRMSLAAIIIAMGMLV
DNAIVVSDSALINMERGMRKRVAIMQACSTTALPLLAATVIAILTFLPIYYSPHITGELLSSLVVVIGVSLMFSWVFALT
QTPFFIQEFVRRPRPEELKAALFDGKYYNRFRNALHWVLRHRSVTIGSLAIMLILSAWSFKFIPKVFVPALEKQYFTVDM
WLPEGTNINETDRMASSLADYIRGHEETEMVSTYIGRTPPRYYLSNVSFGPQSNYAQILVKCKTSKDSKELHALLQDSIR
QKYPEPLIKVNKFELSPLTEAVIEARFLGPDPAVLDSLAGKAIEIMRRNPKVADARNEWGNMSLMIRPVYDPVKAGALGI
TKTQMMQSVKSISDGTPVGIYRDNEKKVPVLLKSEGVILQTNDL

Sequences:

>Translated_764_residues
MKLVKYFLQKKAVTILLLVLILAGGLFSYIKMGKLEDAPFTIKQALVLTPYPGASPSEVQSQVTDVLEESIQSLGELYYL
KTENRTGLSKITVYVKKEIRADEMQQLWDKLRRKVNDVQSKLPAGAGPSVVNDDFGDVLGVFYGLTGKSHTYRELEDEAK
IIKNELLKIKDVAKIEIYGIQTPTIDVSVSPSVMAQSGITTSDIARAFEAQNKVVDAGGIDAGQNRLRIESTGNFYSLDD
IRNLTIVSRSGEHFRLADIAQIEESYQTPASNLMRIDGNPAIGIAISTVPTGNVVDMAEAVKKRIDELSQSMPEGYELTS
IYDQGYESAVANQGFVLNLIISVLTVIAILLFFIGFKNGTLIGSGLIFSIFATLIVMMACGIALQRMSLAAIIIAMGMLV
DNAIVVSDSALINMERGMRKRVAIMQACSTTALPLLAATVIAILTFLPIYYSPHITGELLSSLVVVIGVSLMFSWVFALT
QTPFFIQEFVRRPRPEELKAALFDGKYYNRFRNALHWVLRHRSVTIGSLAIMLILSAWSFKFIPKVFVPALEKQYFTVDM
WLPEGTNINETDRMASSLADYIRGHEETEMVSTYIGRTPPRYYLSNVSFGPQSNYAQILVKCKTSKDSKELHALLQDSIR
QKYPEPLIKVNKFELSPLTEAVIEARFLGPDPAVLDSLAGKAIEIMRRNPKVADARNEWGNMSLMIRPVYDPVKAGALGI
TKTQMMQSVKSISDGTPVGIYRDNEKKVPVLLKSEGVILQTNDL
>Mature_764_residues
MKLVKYFLQKKAVTILLLVLILAGGLFSYIKMGKLEDAPFTIKQALVLTPYPGASPSEVQSQVTDVLEESIQSLGELYYL
KTENRTGLSKITVYVKKEIRADEMQQLWDKLRRKVNDVQSKLPAGAGPSVVNDDFGDVLGVFYGLTGKSHTYRELEDEAK
IIKNELLKIKDVAKIEIYGIQTPTIDVSVSPSVMAQSGITTSDIARAFEAQNKVVDAGGIDAGQNRLRIESTGNFYSLDD
IRNLTIVSRSGEHFRLADIAQIEESYQTPASNLMRIDGNPAIGIAISTVPTGNVVDMAEAVKKRIDELSQSMPEGYELTS
IYDQGYESAVANQGFVLNLIISVLTVIAILLFFIGFKNGTLIGSGLIFSIFATLIVMMACGIALQRMSLAAIIIAMGMLV
DNAIVVSDSALINMERGMRKRVAIMQACSTTALPLLAATVIAILTFLPIYYSPHITGELLSSLVVVIGVSLMFSWVFALT
QTPFFIQEFVRRPRPEELKAALFDGKYYNRFRNALHWVLRHRSVTIGSLAIMLILSAWSFKFIPKVFVPALEKQYFTVDM
WLPEGTNINETDRMASSLADYIRGHEETEMVSTYIGRTPPRYYLSNVSFGPQSNYAQILVKCKTSKDSKELHALLQDSIR
QKYPEPLIKVNKFELSPLTEAVIEARFLGPDPAVLDSLAGKAIEIMRRNPKVADARNEWGNMSLMIRPVYDPVKAGALGI
TKTQMMQSVKSISDGTPVGIYRDNEKKVPVLLKSEGVILQTNDL

Specific function: Participates In The Efflux Of Aminoglycosides. Confers Resistance To A Variety Of These Substances. [C]

COG id: COG0841

COG function: function code V; Cation/multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family [H]

Homologues:

Organism=Escherichia coli, GI1788814, Length=664, Percent_Identity=23.4939759036145, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788390, Length=742, Percent_Identity=23.0458221024259, Blast_Score=155, Evalue=1e-38,
Organism=Escherichia coli, GI1788391, Length=740, Percent_Identity=23.6486486486486, Blast_Score=151, Evalue=2e-37,
Organism=Escherichia coli, GI1786788, Length=747, Percent_Identity=23.4270414993307, Blast_Score=136, Evalue=6e-33,
Organism=Escherichia coli, GI1786667, Length=615, Percent_Identity=22.7642276422764, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1789666, Length=797, Percent_Identity=20.5771643663739, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1789930, Length=633, Percent_Identity=23.0647709320695, Blast_Score=124, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001036
- InterPro:   IPR022831 [H]

Pfam domain/function: PF00873 ACR_tran [H]

EC number: NA

Molecular weight: Translated: 84597; Mature: 84597

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVKYFLQKKAVTILLLVLILAGGLFSYIKMGKLEDAPFTIKQALVLTPYPGASPSEVQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHH
SQVTDVLEESIQSLGELYYLKTENRTGLSKITVYVKKEIRADEMQQLWDKLRRKVNDVQS
HHHHHHHHHHHHHHHHEEEEECCCCCCHHHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHH
KLPAGAGPSVVNDDFGDVLGVFYGLTGKSHTYRELEDEAKIIKNELLKIKDVAKIEIYGI
HCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEC
QTPTIDVSVSPSVMAQSGITTSDIARAFEAQNKVVDAGGIDAGQNRLRIESTGNFYSLDD
CCCEEEEECCHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCEEECCC
IRNLTIVSRSGEHFRLADIAQIEESYQTPASNLMRIDGNPAIGIAISTVPTGNVVDMAEA
CCCEEEEECCCCCEEHHHHHHHHHHHCCCHHHEEEECCCCEEEEEEEECCCCCHHHHHHH
VKKRIDELSQSMPEGYELTSIYDQGYESAVANQGFVLNLIISVLTVIAILLFFIGFKNGT
HHHHHHHHHHHCCCCCEEHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
LIGSGLIFSIFATLIVMMACGIALQRMSLAAIIIAMGMLVDNAIVVSDSALINMERGMRK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHEEHHHHHHH
RVAIMQACSTTALPLLAATVIAILTFLPIYYSPHITGELLSSLVVVIGVSLMFSWVFALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QTPFFIQEFVRRPRPEELKAALFDGKYYNRFRNALHWVLRHRSVTIGSLAIMLILSAWSF
CCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KFIPKVFVPALEKQYFTVDMWLPEGTNINETDRMASSLADYIRGHEETEMVSTYIGRTPP
HHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
RYYLSNVSFGPQSNYAQILVKCKTSKDSKELHALLQDSIRQKYPEPLIKVNKFELSPLTE
HHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHEECCCCCCHHHH
AVIEARFLGPDPAVLDSLAGKAIEIMRRNPKVADARNEWGNMSLMIRPVYDPVKAGALGI
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHCCCCCC
TKTQMMQSVKSISDGTPVGIYRDNEKKVPVLLKSEGVILQTNDL
HHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCEEEEECCC
>Mature Secondary Structure
MKLVKYFLQKKAVTILLLVLILAGGLFSYIKMGKLEDAPFTIKQALVLTPYPGASPSEVQ
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHH
SQVTDVLEESIQSLGELYYLKTENRTGLSKITVYVKKEIRADEMQQLWDKLRRKVNDVQS
HHHHHHHHHHHHHHHHEEEEECCCCCCHHHEEEEHHHHCCHHHHHHHHHHHHHHHHHHHH
KLPAGAGPSVVNDDFGDVLGVFYGLTGKSHTYRELEDEAKIIKNELLKIKDVAKIEIYGI
HCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEC
QTPTIDVSVSPSVMAQSGITTSDIARAFEAQNKVVDAGGIDAGQNRLRIESTGNFYSLDD
CCCEEEEECCHHHHHHCCCCHHHHHHHHHHHCCEEECCCCCCCCCEEEEECCCCEEECCC
IRNLTIVSRSGEHFRLADIAQIEESYQTPASNLMRIDGNPAIGIAISTVPTGNVVDMAEA
CCCEEEEECCCCCEEHHHHHHHHHHHCCCHHHEEEECCCCEEEEEEEECCCCCHHHHHHH
VKKRIDELSQSMPEGYELTSIYDQGYESAVANQGFVLNLIISVLTVIAILLFFIGFKNGT
HHHHHHHHHHHCCCCCEEHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCC
LIGSGLIFSIFATLIVMMACGIALQRMSLAAIIIAMGMLVDNAIVVSDSALINMERGMRK
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCHHEEHHHHHHH
RVAIMQACSTTALPLLAATVIAILTFLPIYYSPHITGELLSSLVVVIGVSLMFSWVFALT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QTPFFIQEFVRRPRPEELKAALFDGKYYNRFRNALHWVLRHRSVTIGSLAIMLILSAWSF
CCCHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
KFIPKVFVPALEKQYFTVDMWLPEGTNINETDRMASSLADYIRGHEETEMVSTYIGRTPP
HHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCC
RYYLSNVSFGPQSNYAQILVKCKTSKDSKELHALLQDSIRQKYPEPLIKVNKFELSPLTE
HHHHCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHEECCCCCCHHHH
AVIEARFLGPDPAVLDSLAGKAIEIMRRNPKVADARNEWGNMSLMIRPVYDPVKAGALGI
HHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCHHCCCCCC
TKTQMMQSVKSISDGTPVGIYRDNEKKVPVLLKSEGVILQTNDL
HHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA