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The map label for this gene is traC [H]

Identifier: 150005894

GI number: 150005894

Start: 4233512

End: 4234837

Strand: Direct

Name: traC [H]

Synonym: BVU_3390

Alternate gene names: 150005894

Gene position: 4233512-4234837 (Clockwise)

Preceding gene: 150005893

Following gene: 150005895

Centisome position: 81.99

GC content: 54.22

Gene sequence:

>1326_bases
ATGGCGTATGGAACTAACACATCAGGCAATGCCGGACAGGTAGCCATCGACCGCTTTGCCGAAATGATGATAGCCCGCAT
GGAGCAGATGAAGGCTTCCGAATGGAAGAAATGCTGGATTGGCGGCGCATCCGGATTTGCAGGTCTCCCGCAGAACGTGG
GAGGCCGCAACTACTCGGGTTCCAACTCCTTCTTCCTCCAGCTGCAGACCGCAGCGCAGGGCTACCAACTACCGGTGTAC
CTCACCTTCAAGCAGGCGCACAACTTGAAGGCCCATATACTGAAAGGTGAAAAGGCTTTTCCGGTGGTCTATTGGGACGT
GCTGGCCAAGGACAAGAACGGCCATAAGGTCAGTTCGGATGAGTATAAGGCCATGAGCAAGGAGGAGAAGAAAGGCATGG
ATGTCATTCCCTTCTTCAAGGCCTTCCCCGTATATAACATCGACCAGACCAACCTGGCAGAGGTGCAGCCCGAACGGGTG
CAGAAACTCATGGAGAAGTTCAAGGTGCCCGAACTTCGGGATAAGGAAGGCATGTATGCCCATGCCGCCCTCGACCGGAT
GATTGAGACGCAAGGCTGGCTCTGTCCCATACAGGCGAACAAACGTGAGGACGGTGCCTACTATTCTCCGGCTCGCGATA
TCGTGGTACTGCCTATGAAGGAGCAGTTCAACATCGGCAATACCCCCGAGGAAATCTACCGGGGTGGCATGGAATTTTAT
TCCACCATGCTGCACGAGATGACGCACTCCACCATGACTCCCGAACGGCTCAACCGCGAGATGGGCGGACGCTTCGGAGA
CCTGAAATACGCCAAGGAAGAACTGGTAGCCGAACTGACCGCTGCCATGATCAGCCATTCGATGGGCTTTGACATGAAAG
TGACGGACAATTCCGCAGCTTATTTGGATTCCTGGATTGGGGTATTGAAGCAGGAACCCAAGTTCATCGTTTCCGTCATG
GCGGACGTGAACAAGGCTTCGGACCTGATTCTGGACCATGTGGACAAGCAGCGGCTGGCACTCAACGAGCAGCCTTACCT
GACGAAGAACGACCCGTTTGCCCCGTTGGGCGAAGGCGAAGAAGTGCCGTTCAAGAATGCGGCCATCGTGAAGACAAGGA
ATGGCGACTATGCCATCCGTGCTTCGTATGACGGTGTGGAGTTAGGCTTGAAGAAGGTATCGAAAGATACGGCCAAGACC
TACTTCCAGCTGACGGACTACAAGGACAAGGCAGCCTTCCTGAACATGACGGCGCACAAGACATTTGAGCCGGAGATAGC
CAATCTCAAACGTACGCAGGCTGTGGGAGCGAAAATGAGTATCTAA

Upstream 100 bases:

>100_bases
CAGACCCACGGCACGGATCCTTCGGATGAATCCGCAGAGTTTTATGTCGAACAGACGGACATTGACCGGATGGACCGTCA
GGTAATGGATGACGAAAGCT

Downstream 100 bases:

>100_bases
TCACACAATCAATACCATGACTGATTACAAGGTAAACTTCAAGGACCTCAAAGCTAAGGTAGGCATCGACGATGTAGCCT
ATGCGCTCGGGTACCGCCTG

Product: putative DNA primase

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MAYGTNTSGNAGQVAIDRFAEMMIARMEQMKASEWKKCWIGGASGFAGLPQNVGGRNYSGSNSFFLQLQTAAQGYQLPVY
LTFKQAHNLKAHILKGEKAFPVVYWDVLAKDKNGHKVSSDEYKAMSKEEKKGMDVIPFFKAFPVYNIDQTNLAEVQPERV
QKLMEKFKVPELRDKEGMYAHAALDRMIETQGWLCPIQANKREDGAYYSPARDIVVLPMKEQFNIGNTPEEIYRGGMEFY
STMLHEMTHSTMTPERLNREMGGRFGDLKYAKEELVAELTAAMISHSMGFDMKVTDNSAAYLDSWIGVLKQEPKFIVSVM
ADVNKASDLILDHVDKQRLALNEQPYLTKNDPFAPLGEGEEVPFKNAAIVKTRNGDYAIRASYDGVELGLKKVSKDTAKT
YFQLTDYKDKAAFLNMTAHKTFEPEIANLKRTQAVGAKMSI

Sequences:

>Translated_441_residues
MAYGTNTSGNAGQVAIDRFAEMMIARMEQMKASEWKKCWIGGASGFAGLPQNVGGRNYSGSNSFFLQLQTAAQGYQLPVY
LTFKQAHNLKAHILKGEKAFPVVYWDVLAKDKNGHKVSSDEYKAMSKEEKKGMDVIPFFKAFPVYNIDQTNLAEVQPERV
QKLMEKFKVPELRDKEGMYAHAALDRMIETQGWLCPIQANKREDGAYYSPARDIVVLPMKEQFNIGNTPEEIYRGGMEFY
STMLHEMTHSTMTPERLNREMGGRFGDLKYAKEELVAELTAAMISHSMGFDMKVTDNSAAYLDSWIGVLKQEPKFIVSVM
ADVNKASDLILDHVDKQRLALNEQPYLTKNDPFAPLGEGEEVPFKNAAIVKTRNGDYAIRASYDGVELGLKKVSKDTAKT
YFQLTDYKDKAAFLNMTAHKTFEPEIANLKRTQAVGAKMSI
>Mature_440_residues
AYGTNTSGNAGQVAIDRFAEMMIARMEQMKASEWKKCWIGGASGFAGLPQNVGGRNYSGSNSFFLQLQTAAQGYQLPVYL
TFKQAHNLKAHILKGEKAFPVVYWDVLAKDKNGHKVSSDEYKAMSKEEKKGMDVIPFFKAFPVYNIDQTNLAEVQPERVQ
KLMEKFKVPELRDKEGMYAHAALDRMIETQGWLCPIQANKREDGAYYSPARDIVVLPMKEQFNIGNTPEEIYRGGMEFYS
TMLHEMTHSTMTPERLNREMGGRFGDLKYAKEELVAELTAAMISHSMGFDMKVTDNSAAYLDSWIGVLKQEPKFIVSVMA
DVNKASDLILDHVDKQRLALNEQPYLTKNDPFAPLGEGEEVPFKNAAIVKTRNGDYAIRASYDGVELGLKKVSKDTAKTY
FQLTDYKDKAAFLNMTAHKTFEPEIANLKRTQAVGAKMSI

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 49615; Mature: 49484

Theoretical pI: Translated: 7.72; Mature: 7.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYGTNTSGNAGQVAIDRFAEMMIARMEQMKASEWKKCWIGGASGFAGLPQNVGGRNYSG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCC
SNSFFLQLQTAAQGYQLPVYLTFKQAHNLKAHILKGEKAFPVVYWDVLAKDKNGHKVSSD
CCEEEEEEEECCCCCEEEEEEEECCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCH
EYKAMSKEEKKGMDVIPFFKAFPVYNIDQTNLAEVQPERVQKLMEKFKVPELRDKEGMYA
HHHHHHHHHHCCCCEECHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCHH
HAALDRMIETQGWLCPIQANKREDGAYYSPARDIVVLPMKEQFNIGNTPEEIYRGGMEFY
HHHHHHHHHHCCCEEECCCCCCCCCCEECCCCCEEEEECHHCCCCCCCHHHHHHCHHHHH
STMLHEMTHSTMTPERLNREMGGRFGDLKYAKEELVAELTAAMISHSMGFDMKVTDNSAA
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
YLDSWIGVLKQEPKFIVSVMADVNKASDLILDHVDKQRLALNEQPYLTKNDPFAPLGEGE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCC
EVPFKNAAIVKTRNGDYAIRASYDGVELGLKKVSKDTAKTYFQLTDYKDKAAFLNMTAHK
CCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCHHEEEEHHCC
TFEPEIANLKRTQAVGAKMSI
CCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AYGTNTSGNAGQVAIDRFAEMMIARMEQMKASEWKKCWIGGASGFAGLPQNVGGRNYSG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCC
SNSFFLQLQTAAQGYQLPVYLTFKQAHNLKAHILKGEKAFPVVYWDVLAKDKNGHKVSSD
CCEEEEEEEECCCCCEEEEEEEECCCCCCEEEEECCCCCCCEEEEEEEECCCCCCCCCCH
EYKAMSKEEKKGMDVIPFFKAFPVYNIDQTNLAEVQPERVQKLMEKFKVPELRDKEGMYA
HHHHHHHHHHCCCCEECHHHHCCCCCCCCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCHH
HAALDRMIETQGWLCPIQANKREDGAYYSPARDIVVLPMKEQFNIGNTPEEIYRGGMEFY
HHHHHHHHHHCCCEEECCCCCCCCCCEECCCCCEEEEECHHCCCCCCCHHHHHHCHHHHH
STMLHEMTHSTMTPERLNREMGGRFGDLKYAKEELVAELTAAMISHSMGFDMKVTDNSAA
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHH
YLDSWIGVLKQEPKFIVSVMADVNKASDLILDHVDKQRLALNEQPYLTKNDPFAPLGEGE
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCC
EVPFKNAAIVKTRNGDYAIRASYDGVELGLKKVSKDTAKTYFQLTDYKDKAAFLNMTAHK
CCCCCCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCCCHHEEEEHHCC
TFEPEIANLKRTQAVGAKMSI
CCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]