Click here to switch to the map view.

The map label for this gene is opuAB [H]

Identifier: 150005778

GI number: 150005778

Start: 4104517

End: 4105341

Strand: Direct

Name: opuAB [H]

Synonym: BVU_3271

Alternate gene names: 150005778

Gene position: 4104517-4105341 (Clockwise)

Preceding gene: 150005777

Following gene: 150005779

Centisome position: 79.5

GC content: 44.73

Gene sequence:

>825_bases
ATGATAAACATAGGAAAATATATAGAAACAGCCATCAACTGGCTCACAGAAAACTTTGCCCCTCTTTTCGACGCCATCAA
TGTTGGAATAGGCGGGTTCATAGATGGGTTCCAAAACATATTGATGTGGATACCTTTTTATGTAACAATAGCATTGCTGG
CCATCCTGGCTTGGTATAAATCGGGAAAAGGAGTCAGCATTTTTACCATTTTAGGATTATTGCTGATTTGGGGAATGGGA
TTTTGGAACGAAACCATGCAGACTTTAGCATTGGTGCTTTCGTCTACCATCATCGCTCTTATCATGGGACTTCCCTTGGG
AATATGGAGCGCAAACAGCAAACGATGTGATAAAATTCTGCATCCTATCCTCGACCTGATGCAAACGATGCCTGCTTTCG
TCTATCTGATTCCTGCCGTACTCTTTTTCGGGCTGGGAACCGTTCCGGGAGCCTTTGCCACCATTATTTTTGCCATGCCT
CCCGTAGTGCGTCTGACCAGCCTGGGTATCAAGCAAGTACCGAAGAATGTTGTAGAAGCTTCGCGCTCTTTTGGTGCGAC
ACCTATGCAATTATTATTCAAGGTACAATTACCGCTGGCATTGCCCACTATCATGACCGGTATAAACCAGACAATCCTGA
TGTCGCTCTCCATGGTGGTCATTGCCGCTATGATTGCAGCCGGAGGGTTAGGGGAAATCGTACTGAAAGGTATCACGCAG
ATGAAAATAGGTTTAGGTTTCGAAGGCGGTATCGCAGTAGTCATCCTGGCCATCATTCTGGATAGAATAACACAAGGACT
TGGAAAACAAAAGAAAGGGAAATAA

Upstream 100 bases:

>100_bases
GCGAACTGAAAGGCTTGGTTCCTCTATCGTCCATCGTAGTAGAGATGACCGGAAAAGATAAAGAAGAAATTAATGAACTT
ATTCAAAACGCTATCGAATT

Downstream 100 bases:

>100_bases
GCATGAAGAAAATACTATATTTTTTGTCTTTCCTCCTGCTGCTTGCCTCTTGCAGCGGGAAAAAAGATGATAAGACAATA
AGCATTGGATACATAAACTG

Product: glycine betaine/L-proline transport system permease

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MINIGKYIETAINWLTENFAPLFDAINVGIGGFIDGFQNILMWIPFYVTIALLAILAWYKSGKGVSIFTILGLLLIWGMG
FWNETMQTLALVLSSTIIALIMGLPLGIWSANSKRCDKILHPILDLMQTMPAFVYLIPAVLFFGLGTVPGAFATIIFAMP
PVVRLTSLGIKQVPKNVVEASRSFGATPMQLLFKVQLPLALPTIMTGINQTILMSLSMVVIAAMIAAGGLGEIVLKGITQ
MKIGLGFEGGIAVVILAIILDRITQGLGKQKKGK

Sequences:

>Translated_274_residues
MINIGKYIETAINWLTENFAPLFDAINVGIGGFIDGFQNILMWIPFYVTIALLAILAWYKSGKGVSIFTILGLLLIWGMG
FWNETMQTLALVLSSTIIALIMGLPLGIWSANSKRCDKILHPILDLMQTMPAFVYLIPAVLFFGLGTVPGAFATIIFAMP
PVVRLTSLGIKQVPKNVVEASRSFGATPMQLLFKVQLPLALPTIMTGINQTILMSLSMVVIAAMIAAGGLGEIVLKGITQ
MKIGLGFEGGIAVVILAIILDRITQGLGKQKKGK
>Mature_274_residues
MINIGKYIETAINWLTENFAPLFDAINVGIGGFIDGFQNILMWIPFYVTIALLAILAWYKSGKGVSIFTILGLLLIWGMG
FWNETMQTLALVLSSTIIALIMGLPLGIWSANSKRCDKILHPILDLMQTMPAFVYLIPAVLFFGLGTVPGAFATIIFAMP
PVVRLTSLGIKQVPKNVVEASRSFGATPMQLLFKVQLPLALPTIMTGINQTILMSLSMVVIAAMIAAGGLGEIVLKGITQ
MKIGLGFEGGIAVVILAIILDRITQGLGKQKKGK

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=274, Percent_Identity=51.4598540145985, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1788451, Length=197, Percent_Identity=32.48730964467, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788449, Length=206, Percent_Identity=28.1553398058252, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1786564, Length=155, Percent_Identity=32.258064516129, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29537; Mature: 29537

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIGKYIETAINWLTENFAPLFDAINVGIGGFIDGFQNILMWIPFYVTIALLAILAWYK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKGVSIFTILGLLLIWGMGFWNETMQTLALVLSSTIIALIMGLPLGIWSANSKRCDKIL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
HPILDLMQTMPAFVYLIPAVLFFGLGTVPGAFATIIFAMPPVVRLTSLGIKQVPKNVVEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SRSFGATPMQLLFKVQLPLALPTIMTGINQTILMSLSMVVIAAMIAAGGLGEIVLKGITQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
MKIGLGFEGGIAVVILAIILDRITQGLGKQKKGK
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MINIGKYIETAINWLTENFAPLFDAINVGIGGFIDGFQNILMWIPFYVTIALLAILAWYK
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGKGVSIFTILGLLLIWGMGFWNETMQTLALVLSSTIIALIMGLPLGIWSANSKRCDKIL
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
HPILDLMQTMPAFVYLIPAVLFFGLGTVPGAFATIIFAMPPVVRLTSLGIKQVPKNVVEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
SRSFGATPMQLLFKVQLPLALPTIMTGINQTILMSLSMVVIAAMIAAGGLGEIVLKGITQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
MKIGLGFEGGIAVVILAIILDRITQGLGKQKKGK
HHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]