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The map label for this gene is pfl [H]

Identifier: 150005400

GI number: 150005400

Start: 3644616

End: 3646844

Strand: Reverse

Name: pfl [H]

Synonym: BVU_2880

Alternate gene names: 150005400

Gene position: 3646844-3644616 (Counterclockwise)

Preceding gene: 150005403

Following gene: 150005399

Centisome position: 70.63

GC content: 44.32

Gene sequence:

>2229_bases
ATGGAAATGAACAAAGTTTTTAAGGATGGATTGTGGAGTAAGGAAATTAATGTTTCAGACTTCGTATATACCAACATTAC
CCCTTATGAGGGCGATGCCTCATTCTTGGCAGGCCCCACAGAGCGAACTAAAAAAGTGTGGAACGAATGCCTCAAGGCAC
TAGAAGAAGAACGTGCCAATAACGGAGTTCGTTCCCTTGATAATGTAACAGTATCTACTATCACCTCACATAAAGCAGGA
TACATTGACCGTGAAAACGAACTGATTGTAGGTCTTCAGACGGACGAACTTCTGCGCCGTGCCATCAAACCTTACGGTGG
TATCAAGGTCGTAGAAAAAGCCTGCCATGAAAACGGTGTGGAAGTAGATGAAAAAGTAAAGGATATCTTTACTCATTATC
GCAAAACCCATAATGATGGCGTATTTGATGCTTATACCGAAGAAATCCGCTCTTTCCGTTCACTCGGTTTCCTGACCGGT
CTGCCCGATAACTATGCACGCGGACGCATTATTGGTGACTACCGCCGTTTGGCACTGTATGGTATCGACCGACTGATTGA
AGCAAAACAAGAAGATTTGCGCAATATCACCAGTCCGATGACCGATGCCCGTATCCGCCTGCGCGAAGAAGTAGCCGAAC
AAATTAAAGCACTGAAAGAAATAAAAATATTGGGTGAATATTACGGACTCGATTTGAGCCGTCCCGCTACTAATGCACAA
GAAGCGGTTCAGTGGGTTTATATGGCATATCTTGCTGCCGTGAAAGAACAAGATGGAGCAGCTATGTCATTGGGCAACGT
TTCTTCATTCCTGGATATCTATATTCAATATGATTTGGATCATGGTAAAATTGACGAGTCATTCGCTCAGGAGCTAGTGG
ATCAGTTTATCATCAAGTTACGTATGGTCCGCCATCTGCGTATGCAATCATACAATGACATCTTTGCAGGTGACCCCACT
TGGGTGACCGAATCTATCGGCGGACGATTCAACGACGGACGCACCAAAGTAACAAAAACTTCTTTCCGCTTCTTACAAAC
ACTATACAATCTGGGTCCGTCACCTGAACCCAATATGACTGTACTGTGGAGTCCGGAGTTGCCGGAAGGTTTCAAAGAAT
TCTGCGCACAGGTTTCTATCGACACTTCTTCCATACAGTACGAAAACGATACGCTGATGCGCGAAGTGCGTAACTGTGAC
GATTATGGCATTGCCTGCTGTGTGTCTTATCAGGCTATCGGCAAACAAATCCAATTCTTCGGGGCACGCTGTAATCTGGC
TAAGGCTCTGTTACTAGCTATCAATGGCGGACGTTGCGAAAATACAGGAACTGTAATGGTGAAAGATATTCCTGTATTGA
CAGGAGACTTGCTGAACTTTGAAGAAGTATGGTCTAATTATAAGAAAGTATTAATGCAGATTGCACGTGTGTATAATGAT
GCGATGAATATCATTCACTATATGCATGACAAATATTATTACGAAAAGGCTCAAATGGCATTCATTGATACCGATCCACG
TATCAATCTGGCATATGGCGTAGCCGGTCTGTCCATTGCCATTGACTCATTGTCTGCCATCAAATACGGCAAGGTACGTG
CCAAACGTAATGATATAGGTTTGACTGAAGGCTTTGATATTGAAAATACATATCCTTGCTTCGGTAATGATGATGACCGC
GTAGACCACTTAGGAGTAGATTTGGTTTATTTCTTCAGTGAAGAATTAAAGAAACATCCGGTATACAAAAACGCCCGTCC
TACCCTGTCACTGCTGACTATCACCTCCAATGTAATGTACGGTAAGAAAACAGGTGCCACTCCTGACGGTCGTGCCAAAG
GGGTTGCTTTCGCTCCGGGTGCCAACCCGATGCACGGACGTGATAAGAACGGTGCCATCGCCTCATTGAGTTCTGTAGCC
AAACTACGCTATCGTGATGCTCAGGACGGCATCAGTAACACATTCTCTATCGTACCAAAATCACTAGGAGTGGATCGGGA
AACCCGCATAGAGAATCTGATAACCATGATGGACGGTTATTTCACCAAAGGGGCACACCACCTGAATGTAAATGTGCTGA
ACCGTGAAATGCTGGAAGATGCAATGGAGCATCCTGAAAAATACCCGCAACTTACTATCCGCGTATCAGGTTATGCCGTA
AATTTCATCAAATTGAGCCGTGAACATCAGCTGGAAGTAATCAGCCGTTCTTTCCACGAAAGAATGTAA

Upstream 100 bases:

>100_bases
TAAAAATGCACAAGTATTCGCATTATACCTACTAATAGTGATGCATAGCTCACAAAAAAGGAGTACCTTTGCAGCCCTAA
ATCATCTTAATAATGACAGT

Downstream 100 bases:

>100_bases
CATCACATATACATTAATCGTAGGGGCGGGTTTTAAACCTGCCCTTACCAGTTTAAAGACAAACCATTATGATCAGAGTA
CATTCTTACGAGTCAATGGG

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase [H]

Number of amino acids: Translated: 742; Mature: 742

Protein sequence:

>742_residues
MEMNKVFKDGLWSKEINVSDFVYTNITPYEGDASFLAGPTERTKKVWNECLKALEEERANNGVRSLDNVTVSTITSHKAG
YIDRENELIVGLQTDELLRRAIKPYGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTG
LPDNYARGRIIGDYRRLALYGIDRLIEAKQEDLRNITSPMTDARIRLREEVAEQIKALKEIKILGEYYGLDLSRPATNAQ
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDHGKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPT
WVTESIGGRFNDGRTKVTKTSFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNCD
DYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLLNFEEVWSNYKKVLMQIARVYND
AMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGLSIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENTYPCFGNDDDR
VDHLGVDLVYFFSEELKKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVA
KLRYRDAQDGISNTFSIVPKSLGVDRETRIENLITMMDGYFTKGAHHLNVNVLNREMLEDAMEHPEKYPQLTIRVSGYAV
NFIKLSREHQLEVISRSFHERM

Sequences:

>Translated_742_residues
MEMNKVFKDGLWSKEINVSDFVYTNITPYEGDASFLAGPTERTKKVWNECLKALEEERANNGVRSLDNVTVSTITSHKAG
YIDRENELIVGLQTDELLRRAIKPYGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTG
LPDNYARGRIIGDYRRLALYGIDRLIEAKQEDLRNITSPMTDARIRLREEVAEQIKALKEIKILGEYYGLDLSRPATNAQ
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDHGKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPT
WVTESIGGRFNDGRTKVTKTSFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNCD
DYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLLNFEEVWSNYKKVLMQIARVYND
AMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGLSIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENTYPCFGNDDDR
VDHLGVDLVYFFSEELKKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVA
KLRYRDAQDGISNTFSIVPKSLGVDRETRIENLITMMDGYFTKGAHHLNVNVLNREMLEDAMEHPEKYPQLTIRVSGYAV
NFIKLSREHQLEVISRSFHERM
>Mature_742_residues
MEMNKVFKDGLWSKEINVSDFVYTNITPYEGDASFLAGPTERTKKVWNECLKALEEERANNGVRSLDNVTVSTITSHKAG
YIDRENELIVGLQTDELLRRAIKPYGGIKVVEKACHENGVEVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTG
LPDNYARGRIIGDYRRLALYGIDRLIEAKQEDLRNITSPMTDARIRLREEVAEQIKALKEIKILGEYYGLDLSRPATNAQ
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDHGKIDESFAQELVDQFIIKLRMVRHLRMQSYNDIFAGDPT
WVTESIGGRFNDGRTKVTKTSFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNCD
DYGIACCVSYQAIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLLNFEEVWSNYKKVLMQIARVYND
AMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGLSIAIDSLSAIKYGKVRAKRNDIGLTEGFDIENTYPCFGNDDDR
VDHLGVDLVYFFSEELKKHPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVA
KLRYRDAQDGISNTFSIVPKSLGVDRETRIENLITMMDGYFTKGAHHLNVNVLNREMLEDAMEHPEKYPQLTIRVSGYAV
NFIKLSREHQLEVISRSFHERM

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=748, Percent_Identity=57.620320855615, Blast_Score=856, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=746, Percent_Identity=54.6916890080429, Blast_Score=818, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=590, Percent_Identity=26.4406779661017, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1790388, Length=745, Percent_Identity=23.8926174496644, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=66.6666666666667, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84086; Mature: 84086

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMNKVFKDGLWSKEINVSDFVYTNITPYEGDASFLAGPTERTKKVWNECLKALEEERAN
CCCHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
NGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKPYGGIKVVEKACHENGV
CCHHHHCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCHHHHHHHHHCCCC
EVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRLALY
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
GIDRLIEAKQEDLRNITSPMTDARIRLREEVAEQIKALKEIKILGEYYGLDLSRPATNAQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDHGKIDESFAQELVDQFIIKL
HHHHHHHHHHHHHHHCCCCCEEECCCHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
RMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKTSFRFLQTLYNLGPSPEPNMT
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
VLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNCDDYGIACCVSYQAIGKQIQFF
EEECCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
GARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLLNFEEVWSNYKKVLMQIARVYND
HHHHHHHHHHHHEECCCEECCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGLSIAIDSLSAIKYGKVRAKRNDIG
HHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHEECCCCCC
LTEGFDIENTYPCFGNDDDRVDHLGVDLVYFFSEELKKHPVYKNARPTLSLLTITSNVMY
CCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEE
GKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDAQDGISNTFSIVPK
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCHHHHHHHHHH
SLGVDRETRIENLITMMDGYFTKGAHHLNVNVLNREMLEDAMEHPEKYPQLTIRVSGYAV
HCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCHHCCCEEEEEEECEEE
NFIKLSREHQLEVISRSFHERM
EEEEECCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEMNKVFKDGLWSKEINVSDFVYTNITPYEGDASFLAGPTERTKKVWNECLKALEEERAN
CCCHHHHHCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHC
NGVRSLDNVTVSTITSHKAGYIDRENELIVGLQTDELLRRAIKPYGGIKVVEKACHENGV
CCHHHHCCCEEEEECCCCCCCCCCCCCEEEEECHHHHHHHHHCCCCCHHHHHHHHHCCCC
EVDEKVKDIFTHYRKTHNDGVFDAYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRLALY
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHH
GIDRLIEAKQEDLRNITSPMTDARIRLREEVAEQIKALKEIKILGEYYGLDLSRPATNAQ
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHH
EAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYIQYDLDHGKIDESFAQELVDQFIIKL
HHHHHHHHHHHHHHHCCCCCEEECCCHHHEEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
RMVRHLRMQSYNDIFAGDPTWVTESIGGRFNDGRTKVTKTSFRFLQTLYNLGPSPEPNMT
HHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEE
VLWSPELPEGFKEFCAQVSIDTSSIQYENDTLMREVRNCDDYGIACCVSYQAIGKQIQFF
EEECCCCCHHHHHHHHHCCCCCCCEEECCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHH
GARCNLAKALLLAINGGRCENTGTVMVKDIPVLTGDLLNFEEVWSNYKKVLMQIARVYND
HHHHHHHHHHHHEECCCEECCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHHHHHH
AMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGLSIAIDSLSAIKYGKVRAKRNDIG
HHHHHHHHHHHHHHHHHEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHEECCCCCC
LTEGFDIENTYPCFGNDDDRVDHLGVDLVYFFSEELKKHPVYKNARPTLSLLTITSNVMY
CCCCCCCCCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCEEE
GKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDAQDGISNTFSIVPK
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHCCHHHHHHHHHH
SLGVDRETRIENLITMMDGYFTKGAHHLNVNVLNREMLEDAMEHPEKYPQLTIRVSGYAV
HCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCHHCCCEEEEEEECEEE
NFIKLSREHQLEVISRSFHERM
EEEEECCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8636053 [H]