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The map label for this gene is pflA [H]

Identifier: 150005399

GI number: 150005399

Start: 3643819

End: 3644547

Strand: Reverse

Name: pflA [H]

Synonym: BVU_2879

Alternate gene names: 150005399

Gene position: 3644547-3643819 (Counterclockwise)

Preceding gene: 150005400

Following gene: 150005391

Centisome position: 70.59

GC content: 40.05

Gene sequence:

>729_bases
ATGATCAGAGTACATTCTTACGAGTCAATGGGGACGTTTGACGGTCCCGGCTTACGCTTAGTCGTATTTTTACAAGGATG
CAATTTCCGATGTCTCTATTGTGCCAACCCCGACACAATTGACACCAAAGGTGAAAGTACGGAAACGGCAATAGACGAAA
TTGTGCGTATGGCTGTCAGCCAAAAAGCTTTTTTCGGTAAAAAAGGAGGTGTGACTTTCAGCGGCGGCGAACCTACATTG
CAAGCAAAAGCACTGATTCCGTTATTCCAGCGGTTAAAAGAACAAAACATCCACATCTGTATAGATACCAACGGCAGTAT
ATGGAATGAGGAAGTAGAGGAACTGCTAAAATGGACAGACCTCGTATTATTAGATATCAAGGAATTTAATAATGTGCGTC
ATCGGCAACTGACAGAACGTAGCAACGAACAAACCATACGTACCGCTGAATGGCTTGAAAAAAACGGAAAACCTTTTTGG
TTGCGCTACGTATTAGTTCCAGGCTATAGTTCCTTTGAGGAAGACATACGGGCATTAGGAGAGCACTTCAAGAACTATCA
CATGATTCAAAGAGTAGAAATACTACCTTATCATACATTAGGCGTACACAAATATGAAGCCATGGGAAAAGAATACAAAC
TGAACGGAATAAAAGAAAATACTTTAGAACAATTGGAAACAGCCAAAACGTTATTTGGTGAATATTTCAATACCGTATAT
TTAAATTAG

Upstream 100 bases:

>100_bases
TAATCAGCCGTTCTTTCCACGAAAGAATGTAACATCACATATACATTAATCGTAGGGGCGGGTTTTAAACCTGCCCTTAC
CAGTTTAAAGACAAACCATT

Downstream 100 bases:

>100_bases
AAAAGACAAACTTACCGTCTATCCGGTACTTAAGTTCCGTTAAGGAGATGAGCCCCATAATTTTCGCCTCATCTCCTTTT
TATGTATAAGAAACAATATA

Product: putative pyruvate formate-lyase 1 activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGESTETAIDEIVRMAVSQKAFFGKKGGVTFSGGEPTL
QAKALIPLFQRLKEQNIHICIDTNGSIWNEEVEELLKWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAEWLEKNGKPFW
LRYVLVPGYSSFEEDIRALGEHFKNYHMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTLEQLETAKTLFGEYFNTVY
LN

Sequences:

>Translated_242_residues
MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGESTETAIDEIVRMAVSQKAFFGKKGGVTFSGGEPTL
QAKALIPLFQRLKEQNIHICIDTNGSIWNEEVEELLKWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAEWLEKNGKPFW
LRYVLVPGYSSFEEDIRALGEHFKNYHMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTLEQLETAKTLFGEYFNTVY
LN
>Mature_242_residues
MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGESTETAIDEIVRMAVSQKAFFGKKGGVTFSGGEPTL
QAKALIPLFQRLKEQNIHICIDTNGSIWNEEVEELLKWTDLVLLDIKEFNNVRHRQLTERSNEQTIRTAEWLEKNGKPFW
LRYVLVPGYSSFEEDIRALGEHFKNYHMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTLEQLETAKTLFGEYFNTVY
LN

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=240, Percent_Identity=42.5, Blast_Score=207, Evalue=6e-55,
Organism=Escherichia coli, GI1790389, Length=259, Percent_Identity=23.9382239382239, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790839, Length=237, Percent_Identity=28.6919831223629, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI226510931, Length=164, Percent_Identity=25.609756097561, Blast_Score=68, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27995; Mature: 27995

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGESTETAIDEIVRMAVS
CEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
QKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQNIHICIDTNGSIWNEEVEELLKWTD
HHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHH
LVLLDIKEFNNVRHRQLTERSNEQTIRTAEWLEKNGKPFWLRYVLVPGYSSFEEDIRALG
HHEEEHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHH
EHFKNYHMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTLEQLETAKTLFGEYFNTVY
HHHHHHHHHHHEEECCCCHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEE
LN
CC
>Mature Secondary Structure
MIRVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIDTKGESTETAIDEIVRMAVS
CEEEECCCCCCCCCCCCEEEEEEEECCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHH
QKAFFGKKGGVTFSGGEPTLQAKALIPLFQRLKEQNIHICIDTNGSIWNEEVEELLKWTD
HHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHH
LVLLDIKEFNNVRHRQLTERSNEQTIRTAEWLEKNGKPFWLRYVLVPGYSSFEEDIRALG
HHEEEHHHHCCHHHHHHHHCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHH
EHFKNYHMIQRVEILPYHTLGVHKYEAMGKEYKLNGIKENTLEQLETAKTLFGEYFNTVY
HHHHHHHHHHHEEECCCCHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEE
LN
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]