Click here to switch to the map view.

The map label for this gene is wcaJ [H]

Identifier: 150005206

GI number: 150005206

Start: 3397560

End: 3398963

Strand: Reverse

Name: wcaJ [H]

Synonym: BVU_2676

Alternate gene names: 150005206

Gene position: 3398963-3397560 (Counterclockwise)

Preceding gene: 150005207

Following gene: 150005205

Centisome position: 65.83

GC content: 45.87

Gene sequence:

>1404_bases
ATGATACAAGTCCAGAGATTCAACAAAGTACTAAAATTAGCAGTCATTCTAGGAGATTTAGTCATTCTGAATGTACTTTT
CATCTCATTTAATTCAATATGGAACGAGTTGTTTGGGGAAAGAACCTGTAGCGGCACTTTACCCCAAATTCTGACTCTGT
TCAGTGTATGCTATTTTGCATGCACTATTTCAGGAGGTGTCATACTGCATCGTCGGGGAGCCCGTGCCGACCAAATTGTA
TTCCGGGTACTCCGCAACGTGTTCTATTTCTCTTTTTTATCGACCGGACTGATGGTATTATCCGAGCTTGAAATATATTC
CAAACGCTTTTTTATCTATTATTTCATTCTGCTCATCCTCTGTATCACTGTCTATCGCCTGCTTTTCCGTTTCTGCATAC
AGCTATACAGAAGCCATGGAGGAAATTTCCGTACCGTCCTCTATTTGGGAAGCACCGAAAATATAGCCGAGCTGTACCAT
GAAATGAGCTCCGACGCTACCACCGGCTACCGGGTACTGGGCTATTTCGACACCACCCCCAATGCCAAATTCCCCGCCAG
TTGTACCTATCTGGGAAAACCGGAGCAAGTTATCAGTTACCTGGCACAGCACAAGGTGAACCAGCTTTACTGCTGCCTGC
CCTCGGCATTGAGCGAACAGATCGTGCCGGTCATCAACTATTGCGAGAACAACCTGATACATTTCTACAGCGTGCCCAAT
GTACGCAACTACCTGCGCCACCGCATGCACTTTGAAATGATAGGCAGCGTGCCCATCCTCAGCATCCGCAAGGAGCCCTT
GGGGAAAATAGAGAACAGAATGTTGAAACGCATATTCGATGTAATCTTCTCACTGCTCTTTTTATGCACATTATTCCCCA
TTATATTCCTTATTGTAGGAGTGACCATCAAAATCACTTCTCCGGGTCCCATATTCTTCCGTCAGAAACGTAACGGATTG
AACAACAAAGAATTCTGGTGCTATAAATTCCGTTCCATGAAAGTCAACAAGGAATCGGACACCTTACAGGCCACCCTCAA
CGATCCCAGGAAAACCAAATTCGGCGATTTCATGCGCCGGACCAATATTGACGAACTTCCGCAATTCATCAATGTATTGC
TGGGAGATATGTCCATTGTAGGGCCACGCCCCCACATGTTGAAACACACGGAAGAATATTCCAAGATAATCAATAAATAC
ATGGTCCGCCATTTCATCAAGCCCGGTATCACGGGATGGGCGCAGGTGACCGGTTTCCGTGGCGAAACCCGCGAACTGGA
AGACATGGAAGGACGTGTGAAAGCCGATATATGGTACATGGAGCACTGGTCTTTCCTGCTCGACCTTTATATTATGTACA
AAACCGTGGCAAACGCGCTGCACAAAGACAAGCAAGCTTATTAA

Upstream 100 bases:

>100_bases
TTACAAAGTAACAGAAGAGCAGAAGAAAGAAAAAAAGCGACATAGTTGAAACCTATTGAAAAAAACATCATATAGATCTG
ACATATAAAAAGTTTTACCT

Downstream 100 bases:

>100_bases
AACAAGACCTGAAAACTGAAAAATTTTAAATAACAGAGAGCCAAAAAAAATGAGAAGATTAAAGTTGACCCTATTCGCGC
TGGTTTCCCTGCTGCTATGC

Product: glycosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MIQVQRFNKVLKLAVILGDLVILNVLFISFNSIWNELFGERTCSGTLPQILTLFSVCYFACTISGGVILHRRGARADQIV
FRVLRNVFYFSFLSTGLMVLSELEIYSKRFFIYYFILLILCITVYRLLFRFCIQLYRSHGGNFRTVLYLGSTENIAELYH
EMSSDATTGYRVLGYFDTTPNAKFPASCTYLGKPEQVISYLAQHKVNQLYCCLPSALSEQIVPVINYCENNLIHFYSVPN
VRNYLRHRMHFEMIGSVPILSIRKEPLGKIENRMLKRIFDVIFSLLFLCTLFPIIFLIVGVTIKITSPGPIFFRQKRNGL
NNKEFWCYKFRSMKVNKESDTLQATLNDPRKTKFGDFMRRTNIDELPQFINVLLGDMSIVGPRPHMLKHTEEYSKIINKY
MVRHFIKPGITGWAQVTGFRGETRELEDMEGRVKADIWYMEHWSFLLDLYIMYKTVANALHKDKQAY

Sequences:

>Translated_467_residues
MIQVQRFNKVLKLAVILGDLVILNVLFISFNSIWNELFGERTCSGTLPQILTLFSVCYFACTISGGVILHRRGARADQIV
FRVLRNVFYFSFLSTGLMVLSELEIYSKRFFIYYFILLILCITVYRLLFRFCIQLYRSHGGNFRTVLYLGSTENIAELYH
EMSSDATTGYRVLGYFDTTPNAKFPASCTYLGKPEQVISYLAQHKVNQLYCCLPSALSEQIVPVINYCENNLIHFYSVPN
VRNYLRHRMHFEMIGSVPILSIRKEPLGKIENRMLKRIFDVIFSLLFLCTLFPIIFLIVGVTIKITSPGPIFFRQKRNGL
NNKEFWCYKFRSMKVNKESDTLQATLNDPRKTKFGDFMRRTNIDELPQFINVLLGDMSIVGPRPHMLKHTEEYSKIINKY
MVRHFIKPGITGWAQVTGFRGETRELEDMEGRVKADIWYMEHWSFLLDLYIMYKTVANALHKDKQAY
>Mature_467_residues
MIQVQRFNKVLKLAVILGDLVILNVLFISFNSIWNELFGERTCSGTLPQILTLFSVCYFACTISGGVILHRRGARADQIV
FRVLRNVFYFSFLSTGLMVLSELEIYSKRFFIYYFILLILCITVYRLLFRFCIQLYRSHGGNFRTVLYLGSTENIAELYH
EMSSDATTGYRVLGYFDTTPNAKFPASCTYLGKPEQVISYLAQHKVNQLYCCLPSALSEQIVPVINYCENNLIHFYSVPN
VRNYLRHRMHFEMIGSVPILSIRKEPLGKIENRMLKRIFDVIFSLLFLCTLFPIIFLIVGVTIKITSPGPIFFRQKRNGL
NNKEFWCYKFRSMKVNKESDTLQATLNDPRKTKFGDFMRRTNIDELPQFINVLLGDMSIVGPRPHMLKHTEEYSKIINKY
MVRHFIKPGITGWAQVTGFRGETRELEDMEGRVKADIWYMEHWSFLLDLYIMYKTVANALHKDKQAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=387, Percent_Identity=35.4005167958656, Blast_Score=248, Evalue=5e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54498; Mature: 54498

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQVQRFNKVLKLAVILGDLVILNVLFISFNSIWNELFGERTCSGTLPQILTLFSVCYFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
CTISGGVILHRRGARADQIVFRVLRNVFYFSFLSTGLMVLSELEIYSKRFFIYYFILLIL
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CITVYRLLFRFCIQLYRSHGGNFRTVLYLGSTENIAELYHEMSSDATTGYRVLGYFDTTP
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEEECCC
NAKFPASCTYLGKPEQVISYLAQHKVNQLYCCLPSALSEQIVPVINYCENNLIHFYSVPN
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCC
VRNYLRHRMHFEMIGSVPILSIRKEPLGKIENRMLKRIFDVIFSLLFLCTLFPIIFLIVG
HHHHHHHHHHHHHHCCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VTIKITSPGPIFFRQKRNGLNNKEFWCYKFRSMKVNKESDTLQATLNDPRKTKFGDFMRR
CEEEECCCCCEEEEHHCCCCCCCCEEEEEEECCCCCCCCCHHEEECCCCCCCHHHHHHHH
TNIDELPQFINVLLGDMSIVGPRPHMLKHTEEYSKIINKYMVRHFIKPGITGWAQVTGFR
CCHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
GETRELEDMEGRVKADIWYMEHWSFLLDLYIMYKTVANALHKDKQAY
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIQVQRFNKVLKLAVILGDLVILNVLFISFNSIWNELFGERTCSGTLPQILTLFSVCYFA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
CTISGGVILHRRGARADQIVFRVLRNVFYFSFLSTGLMVLSELEIYSKRFFIYYFILLIL
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CITVYRLLFRFCIQLYRSHGGNFRTVLYLGSTENIAELYHEMSSDATTGYRVLGYFDTTP
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCEEEEEEEECCC
NAKFPASCTYLGKPEQVISYLAQHKVNQLYCCLPSALSEQIVPVINYCENNLIHFYSVPN
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECCC
VRNYLRHRMHFEMIGSVPILSIRKEPLGKIENRMLKRIFDVIFSLLFLCTLFPIIFLIVG
HHHHHHHHHHHHHHCCCCEEHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
VTIKITSPGPIFFRQKRNGLNNKEFWCYKFRSMKVNKESDTLQATLNDPRKTKFGDFMRR
CEEEECCCCCEEEEHHCCCCCCCCEEEEEEECCCCCCCCCHHEEECCCCCCCHHHHHHHH
TNIDELPQFINVLLGDMSIVGPRPHMLKHTEEYSKIINKYMVRHFIKPGITGWAQVTGFR
CCHHHHHHHHHHHHHCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
GETRELEDMEGRVKADIWYMEHWSFLLDLYIMYKTVANALHKDKQAY
CCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]