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The map label for this gene is pckA [H]

Identifier: 148827997

GI number: 148827997

Start: 1388062

End: 1389678

Strand: Reverse

Name: pckA [H]

Synonym: CGSHiGG_07540

Alternate gene names: 148827997

Gene position: 1389678-1388062 (Counterclockwise)

Preceding gene: 148827998

Following gene: 148827996

Centisome position: 73.64

GC content: 39.39

Gene sequence:

>1617_bases
ATGACAGACTTAAATAAAGTGGTAAAAGAACTTGAAGCTCTTGGCATTTATGACGTAAAAGAAGTTGTTTACAATCCAAG
CTACGAGCAATTGTTCGAAGAAGAAACTAAACCAGGTTTAGAAGGCTTTGAAAAAGGTACTTTAACTACGACTGGTGCAG
TGGCAGTAGATACAGGTATCTTCACAGGTCGTTCTCCAAAAGATAAATATATCGTGTTAGATGAAAAAACAAAAGATACT
GTTTGGTGGACATCTGAAACAGCAAAAAACGACAACAAGCCAATGAACCAAGCTACATGGCAAAGCTTAAAAGACTTGGT
AACTAACCAACTTTCTCGTAAACGCTTATTTGTAGTTGATGGTTTCTGTGGTGCGAGCGAACACGACCGTATTGCAGTAC
GTATTGTCACTGAAGTAGCGTGGCAAGCACATTTTGTAAAAAATATGTTTATTCGCCCAACTGAAGAACAACTCAAAAAT
TTTGAACCAGATTTCGTTGTAATGAACGGTTCTAAAGTAACCAATCCAAACTGGAAAGAACAAGGTTTAAATTCAGAAAA
CTTTGTTGCTTTCAACTTGACTGAACGCATTCAATTAATCGGCGGTACTTGGTACGGCGGCGAAATGAAAAAAGGTATGT
TCTCAATGATGAACTACTTCCTACCTCTCAAAGGTGTTGGTGCAATGCACTGCTCAGCTAACGTTGGTAAAGATGGTGAT
GTAGCAATCTTCTTCGGCTTATCTGGCACAGGTAAAACAACCCTTTCAACGGATCCAAAACGTGAATTAATCGGTGACGA
TGAACACGGCTGGGATGATGTGGGTATCTTTAACTTTGAAGGTGGTTGTTATGCGAAAACCATTCATCTTTCAGAAGAAA
ATGAACCAGATATTTACCGCGCTATCCGTCGCGACGCATTATTAGAAAACGTGGTTGTTCGTTCAGATGGTTCTGTTGAT
TTCGATGATGGTTCAAAAACAGAAAATACTCGCGTGTCTTACCCAATTTATCACATCGATAACATTGTAAAACCAGTTTC
TCGTGCAGGTCACGCAACTAAAGTGATTTTCTTAACTGCAGATGCATTTGGCGTATTACCACCAGTATCTAAATTGACAC
CAGAACAAACTAAATACTACTTCTTATCTGGTTTCACTGCAAAATTAGCAGGGACTGAACGTGGTATTACTGAACCAACG
CCAACTTTCTCAGCATGTTTCGGTGCAGCATTTTTAACGCTTCATCCAACTCAATATGCAGAAGTGTTAGTAAAACGTAT
GCAAGCAGCAGGTGCTGAAGCTTACTTAGTGAATACTGGTTGGAATGGCACAGGCAAACGTATCTCAATCAAAGATACTC
GCGGAATCATTGATGCAATCTTAGATGGCTCAATTGAAAAAGCTGAAATGGGCGAATTGCCAATCTTCAACTTAGCCATT
CCTAAAGCATTACCAGGTGTAGATTCTGCAATCTTAGATCCTCGCGATACTTACGCAGATAAAGCACAATGGCAATCAAA
AGCTGAAGACTTAACAGGTCGTTTTGTGAAAAACTTTGTTAAATATGCAACTAACGAAGAAGGCAAAGCTTTAATTGCAG
CTGGTCCTAAAGCTTAA

Upstream 100 bases:

>100_bases
AAAAATGTTTAAAAATGAGATCTACTTAACATTTTTTTATATTGACATCCATTATAATAAACCACAGTTAAATTTTAATC
TTACATGTTAGAGGTGATTT

Downstream 100 bases:

>100_bases
TTAAAAATACTAAAAATATTAACCCTTTGGGTAACTGAAGTGCGGTCATTTTTTATCTGTTTTCAAGCTGATAAATGTAT
TCAAAAATTTAAAAAAATCC

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGIFTGRSPKDKYIVLDEKTKDT
VWWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVDGFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKN
FEPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSMMNYFLPLKGVGAMHCSANVGKDGD
VAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYRAIRRDALLENVVVRSDGSVD
FDDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLTLHPTQYAEVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAI
PKALPGVDSAILDPRDTYADKAQWQSKAEDLTGRFVKNFVKYATNEEGKALIAAGPKA

Sequences:

>Translated_538_residues
MTDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGIFTGRSPKDKYIVLDEKTKDT
VWWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVDGFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKN
FEPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSMMNYFLPLKGVGAMHCSANVGKDGD
VAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYRAIRRDALLENVVVRSDGSVD
FDDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPT
PTFSACFGAAFLTLHPTQYAEVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAI
PKALPGVDSAILDPRDTYADKAQWQSKAEDLTGRFVKNFVKYATNEEGKALIAAGPKA
>Mature_537_residues
TDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGIFTGRSPKDKYIVLDEKTKDTV
WWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVDGFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKNF
EPDFVVMNGSKVTNPNWKEQGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSMMNYFLPLKGVGAMHCSANVGKDGDV
AIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYRAIRRDALLENVVVRSDGSVDF
DDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTADAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTP
TFSACFGAAFLTLHPTQYAEVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAIP
KALPGVDSAILDPRDTYADKAQWQSKAEDLTGRFVKNFVKYATNEEGKALIAAGPKA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=530, Percent_Identity=77.9245283018868, Blast_Score=875, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=504, Percent_Identity=46.4285714285714, Blast_Score=448, Evalue=1e-126,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59495; Mature: 59364

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGI
CCCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHCCCCEEECCCEEEECCE
FTGRSPKDKYIVLDEKTKDTVWWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVD
ECCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
GFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKNFEPDFVVMNGSKVTNPNWKE
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCEECCCCHHH
QGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSMMNYFLPLKGVGAMHCSANVGKDGD
HCCCCCCEEEEEHHHEEEEECCEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
VAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYR
EEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHH
AIRRDALLENVVVRSDGSVDFDDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTA
HHHHHHHHHHHEEECCCCEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEC
DAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLTLHPTQYA
CCCCCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHEEEECCHHHH
EVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAI
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHCCCCCEEEEEC
PKALPGVDSAILDPRDTYADKAQWQSKAEDLTGRFVKNFVKYATNEEGKALIAAGPKA
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
>Mature Secondary Structure 
TDLNKVVKELEALGIYDVKEVVYNPSYEQLFEEETKPGLEGFEKGTLTTTGAVAVDTGI
CCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCCCCHHCCCCEEECCCEEEECCE
FTGRSPKDKYIVLDEKTKDTVWWTSETAKNDNKPMNQATWQSLKDLVTNQLSRKRLFVVD
ECCCCCCCCEEEEECCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEE
GFCGASEHDRIAVRIVTEVAWQAHFVKNMFIRPTEEQLKNFEPDFVVMNGSKVTNPNWKE
CCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHCCCCCEEEECCCEECCCCHHH
QGLNSENFVAFNLTERIQLIGGTWYGGEMKKGMFSMMNYFLPLKGVGAMHCSANVGKDGD
HCCCCCCEEEEEHHHEEEEECCEEECCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCC
VAIFFGLSGTGKTTLSTDPKRELIGDDEHGWDDVGIFNFEGGCYAKTIHLSEENEPDIYR
EEEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCEEEEECCCEEEEEEEECCCCCCHHHH
AIRRDALLENVVVRSDGSVDFDDGSKTENTRVSYPIYHIDNIVKPVSRAGHATKVIFLTA
HHHHHHHHHHHEEECCCCEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEEC
DAFGVLPPVSKLTPEQTKYYFLSGFTAKLAGTERGITEPTPTFSACFGAAFLTLHPTQYA
CCCCCCCCHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCCHHHHHHHHEEEECCHHHH
EVLVKRMQAAGAEAYLVNTGWNGTGKRISIKDTRGIIDAILDGSIEKAEMGELPIFNLAI
HHHHHHHHHCCCCEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCHHHCCCCCEEEEEC
PKALPGVDSAILDPRDTYADKAQWQSKAEDLTGRFVKNFVKYATNEEGKALIAAGPKA
CCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA