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The map label for this gene is ogt [H]

Identifier: 148827604

GI number: 148827604

Start: 947348

End: 947887

Strand: Direct

Name: ogt [H]

Synonym: CGSHiGG_05185

Alternate gene names: 148827604

Gene position: 947348-947887 (Clockwise)

Preceding gene: 148827603

Following gene: 148827605

Centisome position: 50.2

GC content: 41.3

Gene sequence:

>540_bases
ATGACCGCACTTTATTACACTTATTACCCCTCGCCAGTGGGACGGCTTTTGATTTTATCTGACGGCGAGAGCATTACGCA
TATTGATTTTGAAAAAGAGCAATATGCGCCTAATCCAAAATGGCAAAAGCAAGATGAATTGCCTGTTTTTCAAAAAGTGC
GGTTAGCTTTTGAACGATATTTTAATGGGGAAATTGAGCGTTTTTCTGAGATTCCCTTAAAACCAGAAGGCACTGCGTTT
CAACAAGCCATTTGGCAAGCCTTGCGAGAAATTGATTATGGCGAACTTTCAACTTATGGCGAGCTGGCGTTACGGATTAA
TAACCCGAAAGCTGTTCGTGCCGTGGGAGGCGCGGTAGGTAGTAATCCGATTAGTATTATTATTCCTTGTCACCGCATTT
TAGGCAAAGACCGAACTTTAACTGGCTTTGGTGGTGGCTTAGAGGCAAAACGTTTTTTATTACAGCTAGAGAAAATTCCC
TATATTGATAAAGGCACTGAAAACACGAAGCCACGCTTTTTTAAGAAATATCACGAATGA

Upstream 100 bases:

>100_bases
GGCTTAAACTTAAATTATAGTTTCTAATCCGTTAAAAAATTTAGCATAATAAAGCACACTTCCACAGCAAGTGTGCTTTT
TTTATAAAAACAAGGCAAAA

Downstream 100 bases:

>100_bases
TTCCACAAACCCTTGAACAATTGCTTTCCCAAGCGCAATCAATTGCAGGCTTAACTTTTGGGGAGTTAGCGGATGAACTG
CATATTCCTGTTCCGCCAGA

Product: cyclic 3',5'-adenosine monophosphate phosphodiesterase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 179; Mature: 178

Protein sequence:

>179_residues
MTALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWQKQDELPVFQKVRLAFERYFNGEIERFSEIPLKPEGTAF
QQAIWQALREIDYGELSTYGELALRINNPKAVRAVGGAVGSNPISIIIPCHRILGKDRTLTGFGGGLEAKRFLLQLEKIP
YIDKGTENTKPRFFKKYHE

Sequences:

>Translated_179_residues
MTALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWQKQDELPVFQKVRLAFERYFNGEIERFSEIPLKPEGTAF
QQAIWQALREIDYGELSTYGELALRINNPKAVRAVGGAVGSNPISIIIPCHRILGKDRTLTGFGGGLEAKRFLLQLEKIP
YIDKGTENTKPRFFKKYHE
>Mature_178_residues
TALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWQKQDELPVFQKVRLAFERYFNGEIERFSEIPLKPEGTAFQ
QAIWQALREIDYGELSTYGELALRINNPKAVRAVGGAVGSNPISIIIPCHRILGKDRTLTGFGGGLEAKRFLLQLEKIPY
IDKGTENTKPRFFKKYHE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=77, Percent_Identity=42.8571428571429, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1787596, Length=101, Percent_Identity=44.5544554455446, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788542, Length=161, Percent_Identity=34.7826086956522, Blast_Score=94, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=42.5, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=42.5, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=45.1219512195122, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI17137554, Length=119, Percent_Identity=36.1344537815126, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20529; Mature: 20398

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWQKQDELPVFQKVRLAFERY
CCEEEEEECCCCCCEEEEEECCCEEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
FNGEIERFSEIPLKPEGTAFQQAIWQALREIDYGELSTYGELALRINNPKAVRAVGGAVG
HCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEHHHCCCCC
SNPISIIIPCHRILGKDRTLTGFGGGLEAKRFLLQLEKIPYIDKGTENTKPRFFKKYHE
CCCEEEEEECHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure 
TALYYTYYPSPVGRLLILSDGESITHIDFEKEQYAPNPKWQKQDELPVFQKVRLAFERY
CEEEEEECCCCCCEEEEEECCCEEEEEECCHHHCCCCCCCCCCCCCCHHHHHHHHHHHH
FNGEIERFSEIPLKPEGTAFQQAIWQALREIDYGELSTYGELALRINNPKAVRAVGGAVG
HCCCHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEHHHCCCCC
SNPISIIIPCHRILGKDRTLTGFGGGLEAKRFLLQLEKIPYIDKGTENTKPRFFKKYHE
CCCEEEEEECHHHHCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]