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The map label for this gene is tgs1 [H]

Identifier: 148824324

GI number: 148824324

Start: 3508098

End: 3509489

Strand: Reverse

Name: tgs1 [H]

Synonym: TBFG_13151

Alternate gene names: 148824324

Gene position: 3509489-3508098 (Counterclockwise)

Preceding gene: 148824326

Following gene: 148824322

Centisome position: 79.32

GC content: 62.21

Gene sequence:

>1392_bases
ATGAATCACCTAACGACACTTGACGCCGGGTTTCTCAAGGCAGAAGACGTGGATCGGCACGTGAGTCTGGCAATCGGCGC
TCTGGCGGTCATCGAGGGGCCGGCTCCCGATCAGGAAGCCTTCTTATCGTCGCTCGCTCAACGCCTACGTCCCTGTACCC
GGTTCGGGCAGCGGTTACGCCTGCGCCCGTTCGACCTCGGTGCACCCAAATGGGTGGACGATCCCGACTTCGATCTTGGC
CGTCATGTGTGGCGCATCGCCTTGCCGCGGCCTGGCAACGAAGACCAGTTATTCGAGCTGATCGCCGATCTGATGGCGCG
TCGTTTGGACCGGGGTCGACCGCTGTGGGAGGTCTGGGTCATCGAAGGCCTGGCGGACAGCAAGTGGGCGATCCTGACCA
AACTGCACCACTGCATGGCCGACGGAATCGCGGCGACTCACCTGCTAGCTGGGCTCTCCGATGAAAGTATGAGCGACAGC
TTCGCGAGCAACATCCACACGACCATGCAGTCGCAATCCGCATCTGTGCGGCGGGGTGGATTCCGTGTCAATCCAAGCGA
GGCGTTGACCGCGTCGACCGCCGTGATGGCAGGCATCGTTCGCGCGGCCAAGGGTGCCAGTGAGATCGCGGCCGGCGTGC
TAAGTCCCGCCGCGTCGTCGTTGAACGGGCCGATCAGTGATTTGCGTCGCTACAGCGCAGCAAAGGTCGCTCTCGCCGAC
GTCGAACAGGTGTGCCGGAAATTCGACGTCACCATCAATGATGTTGCGCTTGCCGCGATTACGGAAAGCTACCGCAACGT
CCTCATCCAGCGGGGTGAGCGGCCTAGGTTTGATTCGCTGCGTACGCTAGTGCCGGTCTCGACGCGTTCCAACAGCGCTT
TGAGCAAGACCGATAACCGTGTTTCGTTAATGCTGCCCAACCTGCCGGTGGATCAAGAGAACCCGCTGCAGCGGCTGCGG
ATCGTGCACTCGCGGCTGACTCGGGCCAAGGCGGGGGGACAGAGACAATTCGGAAATACTTTGATGGCGATTGCCAACCG
CCTTCCGTTCCCCATGACCGCATGGGCGGTCGGGCTGTTGATGCGGCTGCCGCAGCGTGGTGTTGTCACCGTGGCGACAA
ATGTGCCGGGTCCACGACGGCCGCTGCAGATTATGGGCAGACGGGTGCTTGACCTATACCCGGTTTCGCCGATCGCGATG
CAACTGCGCACCAGTGTCGCGATGCTCAGCTACGCCGACGACCTGTACTTCGGGATCCTGGCCGACTACGACGTGGTAGC
AGATGCCGGCCAGCTGGCGCGAGGAATTGAAGACGCCGTCGCACGGCTGGTGGCGATCAGTAAGCGGCGCAAGGTGACTC
GCAGGCGCGGAGCGCTATCGCTGGTTGTGTGA

Upstream 100 bases:

>100_bases
CGCCAGCGGAGGACCTTTGGCCCTGCCTCAACGGCTCCTCGCAGCGGAGAGTGGTACTGACGGTTGCAGATAATCCGGAT
CACCGGGGAAGGCGCTGACC

Downstream 100 bases:

>100_bases
CGAGGCCTCGCAGGCAAAGGTCTGGCTGCGGTCCGGTTCCGGACCGAACCTGAAGTGGCGGCCGGTGATCTCCCACGGGA
TCACCCGCACACAGTGACGT

Product: hypothetical protein

Products: NA

Alternate protein names: TGS1; Probable triacylglycerol synthase tgs1 [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MNHLTTLDAGFLKAEDVDRHVSLAIGALAVIEGPAPDQEAFLSSLAQRLRPCTRFGQRLRLRPFDLGAPKWVDDPDFDLG
RHVWRIALPRPGNEDQLFELIADLMARRLDRGRPLWEVWVIEGLADSKWAILTKLHHCMADGIAATHLLAGLSDESMSDS
FASNIHTTMQSQSASVRRGGFRVNPSEALTASTAVMAGIVRAAKGASEIAAGVLSPAASSLNGPISDLRRYSAAKVALAD
VEQVCRKFDVTINDVALAAITESYRNVLIQRGERPRFDSLRTLVPVSTRSNSALSKTDNRVSLMLPNLPVDQENPLQRLR
IVHSRLTRAKAGGQRQFGNTLMAIANRLPFPMTAWAVGLLMRLPQRGVVTVATNVPGPRRPLQIMGRRVLDLYPVSPIAM
QLRTSVAMLSYADDLYFGILADYDVVADAGQLARGIEDAVARLVAISKRRKVTRRRGALSLVV

Sequences:

>Translated_463_residues
MNHLTTLDAGFLKAEDVDRHVSLAIGALAVIEGPAPDQEAFLSSLAQRLRPCTRFGQRLRLRPFDLGAPKWVDDPDFDLG
RHVWRIALPRPGNEDQLFELIADLMARRLDRGRPLWEVWVIEGLADSKWAILTKLHHCMADGIAATHLLAGLSDESMSDS
FASNIHTTMQSQSASVRRGGFRVNPSEALTASTAVMAGIVRAAKGASEIAAGVLSPAASSLNGPISDLRRYSAAKVALAD
VEQVCRKFDVTINDVALAAITESYRNVLIQRGERPRFDSLRTLVPVSTRSNSALSKTDNRVSLMLPNLPVDQENPLQRLR
IVHSRLTRAKAGGQRQFGNTLMAIANRLPFPMTAWAVGLLMRLPQRGVVTVATNVPGPRRPLQIMGRRVLDLYPVSPIAM
QLRTSVAMLSYADDLYFGILADYDVVADAGQLARGIEDAVARLVAISKRRKVTRRRGALSLVV
>Mature_463_residues
MNHLTTLDAGFLKAEDVDRHVSLAIGALAVIEGPAPDQEAFLSSLAQRLRPCTRFGQRLRLRPFDLGAPKWVDDPDFDLG
RHVWRIALPRPGNEDQLFELIADLMARRLDRGRPLWEVWVIEGLADSKWAILTKLHHCMADGIAATHLLAGLSDESMSDS
FASNIHTTMQSQSASVRRGGFRVNPSEALTASTAVMAGIVRAAKGASEIAAGVLSPAASSLNGPISDLRRYSAAKVALAD
VEQVCRKFDVTINDVALAAITESYRNVLIQRGERPRFDSLRTLVPVSTRSNSALSKTDNRVSLMLPNLPVDQENPLQRLR
IVHSRLTRAKAGGQRQFGNTLMAIANRLPFPMTAWAVGLLMRLPQRGVVTVATNVPGPRRPLQIMGRRVLDLYPVSPIAM
QLRTSVAMLSYADDLYFGILADYDVVADAGQLARGIEDAVARLVAISKRRKVTRRRGALSLVV

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Prefers C(26:0)-CoA over C(18:1)-CoA. TG synthesis activity increases in M.tuberculosis upon oxygen depletion and NO

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014292
- InterPro:   IPR004255 [H]

Pfam domain/function: PF03007 UPF0089 [H]

EC number: =2.3.1.20 [H]

Molecular weight: Translated: 50695; Mature: 50695

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLTTLDAGFLKAEDVDRHVSLAIGALAVIEGPAPDQEAFLSSLAQRLRPCTRFGQRLR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LRPFDLGAPKWVDDPDFDLGRHVWRIALPRPGNEDQLFELIADLMARRLDRGRPLWEVWV
CCCCCCCCCCCCCCCCCHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
IEGLADSKWAILTKLHHCMADGIAATHLLAGLSDESMSDSFASNIHTTMQSQSASVRRGG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHCCC
FRVNPSEALTASTAVMAGIVRAAKGASEIAAGVLSPAASSLNGPISDLRRYSAAKVALAD
EEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VEQVCRKFDVTINDVALAAITESYRNVLIQRGERPRFDSLRTLVPVSTRSNSALSKTDNR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEECCCCCCCHHCCCCE
VSLMLPNLPVDQENPLQRLRIVHSRLTRAKAGGQRQFGNTLMAIANRLPFPMTAWAVGLL
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
MRLPQRGVVTVATNVPGPRRPLQIMGRRVLDLYPVSPIAMQLRTSVAMLSYADDLYFGIL
HHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ADYDVVADAGQLARGIEDAVARLVAISKRRKVTRRRGALSLVV
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure
MNHLTTLDAGFLKAEDVDRHVSLAIGALAVIEGPAPDQEAFLSSLAQRLRPCTRFGQRLR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCC
LRPFDLGAPKWVDDPDFDLGRHVWRIALPRPGNEDQLFELIADLMARRLDRGRPLWEVWV
CCCCCCCCCCCCCCCCCHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
IEGLADSKWAILTKLHHCMADGIAATHLLAGLSDESMSDSFASNIHTTMQSQSASVRRGG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHCCC
FRVNPSEALTASTAVMAGIVRAAKGASEIAAGVLSPAASSLNGPISDLRRYSAAKVALAD
EEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VEQVCRKFDVTINDVALAAITESYRNVLIQRGERPRFDSLRTLVPVSTRSNSALSKTDNR
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHEEEECCCCCCCHHCCCCE
VSLMLPNLPVDQENPLQRLRIVHSRLTRAKAGGQRQFGNTLMAIANRLPFPMTAWAVGLL
EEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHH
MRLPQRGVVTVATNVPGPRRPLQIMGRRVLDLYPVSPIAMQLRTSVAMLSYADDLYFGIL
HHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ADYDVVADAGQLARGIEDAVARLVAISKRRKVTRRRGALSLVV
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]