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The map label for this gene is etfA

Identifier: 148824219

GI number: 148824219

Start: 3398785

End: 3399741

Strand: Reverse

Name: etfA

Synonym: TBFG_13044

Alternate gene names: 148824219

Gene position: 3399741-3398785 (Counterclockwise)

Preceding gene: 148824220

Following gene: 148824218

Centisome position: 76.84

GC content: 69.38

Gene sequence:

>957_bases
ATGGCTGAAGTACTGGTGCTCGTTGAGCACGCTGAAGGCGCGTTAAAGAAGGTCAGCGCCGAATTGATCACCGCCGCCCG
CGCCTTGGGCGAACCAGCCGCCGTCGTCGTCGGTGTGCCGGGGACGGCCGCGCCGCTGGTGGACGGGCTTAAGGCGGCTG
GTGCCGCCAAGATCTACGTCGCCGAGTCCGACCTTGTCGACAAATACCTGATCACCCCGGCGGTCGACGTGCTGGCCGGG
CTGGCCGAGTCCTCGGCCCCTGCCGGCGTACTAATCGCCGCCACCGCGGACGGCAAGGAGATCGCCGGCCGACTTGCGGC
TCGGATCGGCTCGGGTCTGCTGGTCGACGTGGTCGACGTGAGAGAAGGTGGAGTGGGTGTCCACAGCATCTTCGGTGGGG
CGTTCACCGTCGAAGCGCAGGCCAACGGCGACACCCCGGTGATCACCGTGCGCGCAGGAGCCGTGGAGGCGGAGCCGGCC
GCCGGCGCCGGTGAGCAGGTCAGCGTGGAAGTGCCGGCTGCGGCGGAGAACGCCGCCAGGATCACCGCGCGCGAACCGGC
GGTCGCCGGCGACCGGCCGGAGCTGACCGAGGCGACCATTGTGGTGGCCGGTGGCCGTGGTGTCGGCAGCGCGGAGAACT
TCAGCGTGGTCGAGGCGCTGGCCGACTCGCTGGGCGCCGCGGTCGGGGCCTCGCGTGCCGCAGTCGACTCCGGCTACTAC
CCGGGCCAGTTCCAGGTCGGCCAGACCGGCAAGACGGTGTCGCCCCAGCTCTACATTGCCCTGGGCATCTCCGGGGCGAT
CCAGCACCGCGCTGGCATGCAGACGTCCAAGACCATCGTCGCGGTCAACAAGGACGAAGAGGCGCCGATCTTTGAGATCG
CCGACTACGGGGTGGTGGGAGACCTGTTCAAGGTCGCTCCGCAGCTGACCGAGGCCATCAAGGCCCGCAAGGGCTAG

Upstream 100 bases:

>100_bases
CCGACGAGGGTGAAGGCGGCAACCAGATCGTGCAGTACCTGGTTGCCCAGAAAATCATCTAAGACATACGCACCTCCCAA
AGACGAGAGCGATATAACCC

Downstream 100 bases:

>100_bases
GTTCCCTGCGCGAGCAGTCGCAAAAGACCCCGACACGCCGAGGCGTGGGGGGCCTTGGCGCCTGCTCACCTAGCCCAACA
GTTCGTCATCTGACGTGCAC

Product: electron transfer flavoprotein alpha subunit fixB

Products: NA

Alternate protein names: Alpha-ETF; Electron transfer flavoprotein large subunit; ETFLS

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MAEVLVLVEHAEGALKKVSAELITAARALGEPAAVVVGVPGTAAPLVDGLKAAGAAKIYVAESDLVDKYLITPAVDVLAG
LAESSAPAGVLIAATADGKEIAGRLAARIGSGLLVDVVDVREGGVGVHSIFGGAFTVEAQANGDTPVITVRAGAVEAEPA
AGAGEQVSVEVPAAAENAARITAREPAVAGDRPELTEATIVVAGGRGVGSAENFSVVEALADSLGAAVGASRAAVDSGYY
PGQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADYGVVGDLFKVAPQLTEAIKARKG

Sequences:

>Translated_318_residues
MAEVLVLVEHAEGALKKVSAELITAARALGEPAAVVVGVPGTAAPLVDGLKAAGAAKIYVAESDLVDKYLITPAVDVLAG
LAESSAPAGVLIAATADGKEIAGRLAARIGSGLLVDVVDVREGGVGVHSIFGGAFTVEAQANGDTPVITVRAGAVEAEPA
AGAGEQVSVEVPAAAENAARITAREPAVAGDRPELTEATIVVAGGRGVGSAENFSVVEALADSLGAAVGASRAAVDSGYY
PGQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADYGVVGDLFKVAPQLTEAIKARKG
>Mature_317_residues
AEVLVLVEHAEGALKKVSAELITAARALGEPAAVVVGVPGTAAPLVDGLKAAGAAKIYVAESDLVDKYLITPAVDVLAGL
AESSAPAGVLIAATADGKEIAGRLAARIGSGLLVDVVDVREGGVGVHSIFGGAFTVEAQANGDTPVITVRAGAVEAEPAA
GAGEQVSVEVPAAAENAARITAREPAVAGDRPELTEATIVVAGGRGVGSAENFSVVEALADSLGAAVGASRAAVDSGYYP
GQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADYGVVGDLFKVAPQLTEAIKARKG

Specific function: The electron transfer flavoprotein serves as a specific electron acceptor for other dehydrogenases. It transfers the electrons to the main respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase)

COG id: COG2025

COG function: function code C; Electron transfer flavoprotein, alpha subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ETF alpha-subunit/fixB family

Homologues:

Organism=Homo sapiens, GI4503607, Length=316, Percent_Identity=42.4050632911392, Blast_Score=222, Evalue=3e-58,
Organism=Homo sapiens, GI189181759, Length=271, Percent_Identity=43.5424354243542, Blast_Score=207, Evalue=7e-54,
Organism=Escherichia coli, GI1787990, Length=240, Percent_Identity=34.1666666666667, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1786226, Length=232, Percent_Identity=37.5, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI87082157, Length=254, Percent_Identity=31.8897637795276, Blast_Score=77, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17506929, Length=316, Percent_Identity=36.7088607594937, Blast_Score=188, Evalue=4e-48,
Organism=Saccharomyces cerevisiae, GI6325261, Length=323, Percent_Identity=34.6749226006192, Blast_Score=170, Evalue=3e-43,
Organism=Drosophila melanogaster, GI17136898, Length=313, Percent_Identity=42.1725239616613, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI24652801, Length=313, Percent_Identity=42.1725239616613, Blast_Score=209, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ETFA_MYCTU (O53275)

Other databases:

- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   G70858
- RefSeq:   NP_217544.1
- RefSeq:   NP_337628.1
- ProteinModelPortal:   O53275
- SMR:   O53275
- EnsemblBacteria:   EBMYCT00000002803
- EnsemblBacteria:   EBMYCT00000069316
- GeneID:   888549
- GeneID:   923026
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3112
- KEGG:   mtu:Rv3028c
- TIGR:   MT3112
- TubercuList:   Rv3028c
- GeneTree:   EBGT00050000017730
- HOGENOM:   HBG615986
- OMA:   PNSTAPE
- ProtClustDB:   CLSK792228
- GO:   GO:0005829
- GO:   GO:0006810
- InterPro:   IPR001308
- InterPro:   IPR014730
- InterPro:   IPR014731
- InterPro:   IPR018206
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF000089
- SMART:   SM00893

Pfam domain/function: PF01012 ETF; PF00766 ETF_alpha

EC number: NA

Molecular weight: Translated: 31691; Mature: 31560

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00696 ETF_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVLVLVEHAEGALKKVSAELITAARALGEPAAVVVGVPGTAAPLVDGLKAAGAAKIYV
CCEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEE
AESDLVDKYLITPAVDVLAGLAESSAPAGVLIAATADGKEIAGRLAARIGSGLLVDVVDV
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEE
REGGVGVHSIFGGAFTVEAQANGDTPVITVRAGAVEAEPAAGAGEQVSVEVPAAAENAAR
CCCCCCHHHCCCCEEEEEEECCCCCCEEEEEECCEECCCCCCCCCEEEEECCCCCCCCEE
ITAREPAVAGDRPELTEATIVVAGGRGVGSAENFSVVEALADSLGAAVGASRAAVDSGYY
EEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCC
PGQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADYGVVG
CCEEEECCCCCCCCCEEEEEEECCHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCHHH
DLFKVAPQLTEAIKARKG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEVLVLVEHAEGALKKVSAELITAARALGEPAAVVVGVPGTAAPLVDGLKAAGAAKIYV
CEEEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCCCEEEEE
AESDLVDKYLITPAVDVLAGLAESSAPAGVLIAATADGKEIAGRLAARIGSGLLVDVVDV
ECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCEEEEEEEE
REGGVGVHSIFGGAFTVEAQANGDTPVITVRAGAVEAEPAAGAGEQVSVEVPAAAENAAR
CCCCCCHHHCCCCEEEEEEECCCCCCEEEEEECCEECCCCCCCCCEEEEECCCCCCCCEE
ITAREPAVAGDRPELTEATIVVAGGRGVGSAENFSVVEALADSLGAAVGASRAAVDSGYY
EEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHCCCCC
PGQFQVGQTGKTVSPQLYIALGISGAIQHRAGMQTSKTIVAVNKDEEAPIFEIADYGVVG
CCEEEECCCCCCCCCEEEEEEECCHHHHHHCCCCCCCEEEEECCCCCCCEEEEECCCHHH
DLFKVAPQLTEAIKARKG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036