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The map label for this gene is ilvB1

Identifier: 148824193

GI number: 148824193

Start: 3372839

End: 3374695

Strand: Reverse

Name: ilvB1

Synonym: TBFG_13018

Alternate gene names: 148824193

Gene position: 3374695-3372839 (Counterclockwise)

Preceding gene: 148824195

Following gene: 148824192

Centisome position: 76.27

GC content: 67.64

Gene sequence:

>1857_bases
GTGAGCGCACCAACCAAGCCACACTCACCGACATTCAAGCCAGAGCCACACAGCGCGGCGAATGAGCCAAAGCACCCGGC
GGCTCGTCCGAAACATGTTGCACTGCAGCAGCTTACCGGTGCACAGGCGGTCATCCGGTCGCTGGAGGAACTCGGCGTCG
ACGTCATTTTCGGGATTCCGGGCGGTGCGGTGCTGCCGGTGTATGACCCGCTGTTCGACTCGAAAAAGCTGCGCCACGTG
CTGGTCCGCCACGAACAGGGCGCCGGGCATGCCGCCAGCGGCTACGCGCACGTCACCGGCCGGGTGGGCGTGTGCATGGC
AACGTCGGGTCCCGGAGCGACCAACCTGGTGACCCCGCTGGCCGACGCGCAGATGGACTCGATCCCGGTGGTCGCTATCA
CCGGTCAGGTCGGGCGGGGGCTGATCGGCACCGACGCCTTCCAGGAGGCCGACATCTCGGGCATCACGATGCCGATCACC
AAGCACAACTTTCTGGTCCGCTCCGGTGACGACATTCCGCGGGTGCTGGCCGAGGCCTTCCACATCGCGGCCTCCGGCCG
TCCGGGCGCGGTGCTGGTCGACATCCCCAAGGACGTGCTGCAGGGCCAGTGCACGTTCAGCTGGCCGCCGCGGATGGAGC
TGCCCGGCTACAAGCCCAACACCAAACCGCACAGCCGGCAGGTCCGCGAGGCCGCCAAGCTGATCGCGGCCGCGCGCAAG
CCGGTGCTGTATGTCGGCGGCGGCGTCATCCGCGGTGAGGCCACCGAGCAGCTCCGGGAGCTGGCCGAGCTGACCGGCAT
CCCGGTGGTCACCACGCTGATGGCCCGCGGCGCGTTTCCCGACAGCCACCGGCAAAACCTCGGCATGCCCGGCATGCACG
GCACGGTGGCCGCCGTGGCGGCGCTGCAGCGCAGCGACCTGCTGATCGCGCTGGGTACCCGCTTCGACGACCGGGTGACC
GGCAAGCTCGACTCGTTCGCGCCGGAAGCCAAGGTCATCCACGCCGACATCGACCCGGCCGAGATCGGCAAGAACCGCCA
CGCCGACGTGCCCATCGTCGGTGACGTCAAGGCCGTCATCACCGAACTGATCGCGATGCTGCGCCACCACCACATTCCCG
GCACCATCGAGATGGCCGACTGGTGGGCATACCTGAACGGTGTGCGCAAGACCTATCCGCTGAGCTATGGGCCGCAGAGC
GACGGCAGCCTGAGCCCGGAATACGTGATCGAAAAGCTCGGCGAGATCGCCGGGCCGGACGCCGTCTTCGTCGCCGGCGT
CGGCCAGCACCAGATGTGGGCCGCGCAGTTCATCAGATACGAAAAGCCGCGCAGCTGGCTGAACTCCGGCGGTCTGGGCA
CCATGGGGTTTGCCATCCCGGCGGCCATGGGCGCCAAGATCGCCCTCCCCGGCACCGAGGTCTGGGCGATCGACGGCGAC
GGTTGCTTCCAGATGACCAACCAGGAGCTGGCCACCTGCGCGGTCGAGGGCATACCGGTCAAGGTGGCGCTGATCAACAA
CGGCAACCTGGGCATGGTGCGGCAGTGGCAGAGCCTGTTCTATGCCGAGCGGTACTCGCAGACCGACCTGGCCACTCATT
CGCACCGCATCCCCGACTTCGTGAAACTGGCCGAGGCCTTGGGGTGTGTGGGGTTGCGGTGCGAGCGGGAAGAGGACGTC
GTCGACGTCATCAACCAGGCGCGGGCGATCAACGACTGCCCGGTGGTGATCGACTTCATCGTCGGTGCCGACGCGCAAGT
GTGGCCGATGGTGGCCGCGGGCACCAGCAATGACGAGATCCAGGCCGCCCGCGGCATTCGCCCCCTGTTCGACGACATCA
CCGAAGGCCACGCCTGA

Upstream 100 bases:

>100_bases
CAAGCGAGCACCAACGCGACCCTCGTGCAGCAGCTGAGCTGGCGAGGGTTTTTTCTTGTCCACCAACGAGACTTCCAGAG
CAATAGAGGATCACACAACA

Downstream 100 bases:

>100_bases
TGAGCCCGAAGACGCACACGTTGTCGGTGTTGGTCGAAGACAAGCCCGGCGTGCTGGCGCGGGTGGCGGCGCTGTTCTCC
CGGCGCGGTTTCAACATCGA

Product: acetolactate synthase 1 catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase large subunit; AHAS

Number of amino acids: Translated: 618; Mature: 617

Protein sequence:

>618_residues
MSAPTKPHSPTFKPEPHSAANEPKHPAARPKHVALQQLTGAQAVIRSLEELGVDVIFGIPGGAVLPVYDPLFDSKKLRHV
LVRHEQGAGHAASGYAHVTGRVGVCMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMPIT
KHNFLVRSGDDIPRVLAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRMELPGYKPNTKPHSRQVREAAKLIAAARK
PVLYVGGGVIRGEATEQLRELAELTGIPVVTTLMARGAFPDSHRQNLGMPGMHGTVAAVAALQRSDLLIALGTRFDDRVT
GKLDSFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKAVITELIAMLRHHHIPGTIEMADWWAYLNGVRKTYPLSYGPQS
DGSLSPEYVIEKLGEIAGPDAVFVAGVGQHQMWAAQFIRYEKPRSWLNSGGLGTMGFAIPAAMGAKIALPGTEVWAIDGD
GCFQMTNQELATCAVEGIPVKVALINNGNLGMVRQWQSLFYAERYSQTDLATHSHRIPDFVKLAEALGCVGLRCEREEDV
VDVINQARAINDCPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDITEGHA

Sequences:

>Translated_618_residues
MSAPTKPHSPTFKPEPHSAANEPKHPAARPKHVALQQLTGAQAVIRSLEELGVDVIFGIPGGAVLPVYDPLFDSKKLRHV
LVRHEQGAGHAASGYAHVTGRVGVCMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMPIT
KHNFLVRSGDDIPRVLAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRMELPGYKPNTKPHSRQVREAAKLIAAARK
PVLYVGGGVIRGEATEQLRELAELTGIPVVTTLMARGAFPDSHRQNLGMPGMHGTVAAVAALQRSDLLIALGTRFDDRVT
GKLDSFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKAVITELIAMLRHHHIPGTIEMADWWAYLNGVRKTYPLSYGPQS
DGSLSPEYVIEKLGEIAGPDAVFVAGVGQHQMWAAQFIRYEKPRSWLNSGGLGTMGFAIPAAMGAKIALPGTEVWAIDGD
GCFQMTNQELATCAVEGIPVKVALINNGNLGMVRQWQSLFYAERYSQTDLATHSHRIPDFVKLAEALGCVGLRCEREEDV
VDVINQARAINDCPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDITEGHA
>Mature_617_residues
SAPTKPHSPTFKPEPHSAANEPKHPAARPKHVALQQLTGAQAVIRSLEELGVDVIFGIPGGAVLPVYDPLFDSKKLRHVL
VRHEQGAGHAASGYAHVTGRVGVCMATSGPGATNLVTPLADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMPITK
HNFLVRSGDDIPRVLAEAFHIAASGRPGAVLVDIPKDVLQGQCTFSWPPRMELPGYKPNTKPHSRQVREAAKLIAAARKP
VLYVGGGVIRGEATEQLRELAELTGIPVVTTLMARGAFPDSHRQNLGMPGMHGTVAAVAALQRSDLLIALGTRFDDRVTG
KLDSFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKAVITELIAMLRHHHIPGTIEMADWWAYLNGVRKTYPLSYGPQSD
GSLSPEYVIEKLGEIAGPDAVFVAGVGQHQMWAAQFIRYEKPRSWLNSGGLGTMGFAIPAAMGAKIALPGTEVWAIDGDG
CFQMTNQELATCAVEGIPVKVALINNGNLGMVRQWQSLFYAERYSQTDLATHSHRIPDFVKLAEALGCVGLRCEREEDVV
DVINQARAINDCPVVIDFIVGADAQVWPMVAAGTSNDEIQAARGIRPLFDDITEGHA

Specific function: Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine. Also involved in condensing pyruvate and 2-ketobutyrate to form 2-

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family

Homologues:

Organism=Homo sapiens, GI93004078, Length=574, Percent_Identity=28.0487804878049, Blast_Score=186, Evalue=5e-47,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=27.4706867671692, Blast_Score=157, Evalue=2e-38,
Organism=Escherichia coli, GI87081685, Length=581, Percent_Identity=46.1273666092943, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI1790104, Length=569, Percent_Identity=44.1124780316344, Blast_Score=451, Evalue=1e-128,
Organism=Escherichia coli, GI1786717, Length=557, Percent_Identity=36.4452423698384, Blast_Score=345, Evalue=5e-96,
Organism=Escherichia coli, GI1787096, Length=559, Percent_Identity=28.4436493738819, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788716, Length=568, Percent_Identity=27.6408450704225, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17531301, Length=567, Percent_Identity=25.7495590828924, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17531299, Length=567, Percent_Identity=25.7495590828924, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17542570, Length=510, Percent_Identity=27.6470588235294, Blast_Score=137, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323755, Length=619, Percent_Identity=42.4878836833603, Blast_Score=459, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6320816, Length=493, Percent_Identity=23.5294117647059, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=501, Percent_Identity=21.9560878243513, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321524, Length=504, Percent_Identity=22.2222222222222, Blast_Score=81, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6323073, Length=501, Percent_Identity=22.5548902195609, Blast_Score=80, Evalue=8e-16,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=26.5377855887522, Blast_Score=187, Evalue=1e-47,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): ILVB1_MYCTU (P0A622)

Other databases:

- EMBL:   BX842581
- EMBL:   AE000516
- PIR:   F70855
- RefSeq:   NP_337598.1
- RefSeq:   YP_177917.1
- ProteinModelPortal:   P0A622
- SMR:   P0A622
- EnsemblBacteria:   EBMYCT00000003781
- EnsemblBacteria:   EBMYCT00000072990
- GeneID:   887286
- GeneID:   926690
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3083
- KEGG:   mtu:Rv3003c
- TIGR:   MT3083
- TubercuList:   Rv3003c
- GeneTree:   EBGT00050000014643
- HOGENOM:   HBG323037
- OMA:   DVAVCMA
- ProtClustDB:   PRK07789
- BRENDA:   2.2.1.6
- GO:   GO:0040007
- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00118

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N

EC number: =2.2.1.6

Molecular weight: Translated: 66123; Mature: 65992

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00187 TPP_ENZYMES

Important sites: BINDING 85-85 BINDING 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPTKPHSPTFKPEPHSAANEPKHPAARPKHVALQQLTGAQAVIRSLEELGVDVIFGIP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECC
GGAVLPVYDPLFDSKKLRHVLVRHEQGAGHAASGYAHVTGRVGVCMATSGPGATNLVTPL
CCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCC
ADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLVRSGDDIPRVLAEAF
CCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHH
HIAASGRPGAVLVDIPKDVLQGQCTFSWPPRMELPGYKPNTKPHSRQVREAAKLIAAARK
HHHCCCCCCEEEEECCHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PVLYVGGGVIRGEATEQLRELAELTGIPVVTTLMARGAFPDSHRQNLGMPGMHGTVAAVA
CEEEECCEEEECHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHH
ALQRSDLLIALGTRFDDRVTGKLDSFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKAVI
HHHHCCEEEEECCCCCCHHCCCHHHCCCCCEEEECCCCHHHCCCCCCCCCCEECCHHHHH
TELIAMLRHHHIPGTIEMADWWAYLNGVRKTYPLSYGPQSDGSLSPEYVIEKLGEIAGPD
HHHHHHHHHCCCCCEEEHHHHHHHHHCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
AVFVAGVGQHQMWAAQFIRYEKPRSWLNSGGLGTMGFAIPAAMGAKIALPGTEVWAIDGD
EEEEECCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCEEECCCCEEEEECCC
GCFQMTNQELATCAVEGIPVKVALINNGNLGMVRQWQSLFYAERYSQTDLATHSHRIPDF
CCEEECCHHHHHHEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHH
VKLAEALGCVGLRCEREEDVVDVINQARAINDCPVVIDFIVGADAQVWPMVAAGTSNDEI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCHH
QAARGIRPLFDDITEGHA
HHHHCCCHHHHHHHCCCC
>Mature Secondary Structure 
SAPTKPHSPTFKPEPHSAANEPKHPAARPKHVALQQLTGAQAVIRSLEELGVDVIFGIP
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHCCCEEEEECC
GGAVLPVYDPLFDSKKLRHVLVRHEQGAGHAASGYAHVTGRVGVCMATSGPGATNLVTPL
CCCEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCC
ADAQMDSIPVVAITGQVGRGLIGTDAFQEADISGITMPITKHNFLVRSGDDIPRVLAEAF
CCCCCCCCCEEEEECCCCCCCCCCCCHHHCCCCCEEEEEECCCEEEECCCCHHHHHHHHH
HIAASGRPGAVLVDIPKDVLQGQCTFSWPPRMELPGYKPNTKPHSRQVREAAKLIAAARK
HHHCCCCCCEEEEECCHHHHCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PVLYVGGGVIRGEATEQLRELAELTGIPVVTTLMARGAFPDSHRQNLGMPGMHGTVAAVA
CEEEECCEEEECHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHCCCCCCCCHHHHHHH
ALQRSDLLIALGTRFDDRVTGKLDSFAPEAKVIHADIDPAEIGKNRHADVPIVGDVKAVI
HHHHCCEEEEECCCCCCHHCCCHHHCCCCCEEEECCCCHHHCCCCCCCCCCEECCHHHHH
TELIAMLRHHHIPGTIEMADWWAYLNGVRKTYPLSYGPQSDGSLSPEYVIEKLGEIAGPD
HHHHHHHHHCCCCCEEEHHHHHHHHHCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCC
AVFVAGVGQHQMWAAQFIRYEKPRSWLNSGGLGTMGFAIPAAMGAKIALPGTEVWAIDGD
EEEEECCCCHHHHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHCCCEEECCCCEEEEECCC
GCFQMTNQELATCAVEGIPVKVALINNGNLGMVRQWQSLFYAERYSQTDLATHSHRIPDF
CCEEECCHHHHHHEECCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHCCCCHHH
VKLAEALGCVGLRCEREEDVVDVINQARAINDCPVVIDFIVGADAQVWPMVAAGTSNDEI
HHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEEECCCCCHH
QAARGIRPLFDDITEGHA
HHHHCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036