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The map label for this gene is speE

Identifier: 148823795

GI number: 148823795

Start: 2941705

End: 2943276

Strand: Direct

Name: speE

Synonym: TBFG_12620

Alternate gene names: 148823795

Gene position: 2941705-2943276 (Clockwise)

Preceding gene: 148823794

Following gene: 148823796

Centisome position: 66.49

GC content: 69.02

Gene sequence:

>1572_bases
ATGACGTCCACCCGGCAGGCGGGCGAAGCCACCGAAGCTTCGGTACGGTGGCGGGCCGTGCTGCTGGCCGCGGTCGCGGC
GTGCGCGGCCTGCGGTCTCGTTTACGAGCTCGCGCTGCTGACACTGGCGGCGAGCCTGAACGGCGGCGGGATCGTGGCCA
CCTCCCTGATCGTCGCGGGCTACATAGCCGCGCTGGGAGCAGGCGCCTTGCTGATCAAGCCGCTACTTGCACACGCGGCC
ATCGCGTTCATCGCCGTGGAGGCGGTGCTGGGCATCATCGGCGGATTGTCCGCGGCGGCGCTGTATGCGGCGTTCGCGTT
CCTGGACGAGCTCGACGGGTCGACGCTGGTTCTTGCGGTGGGCACCGCCCTGATCGGCGGGCTGGTCGGCGCCGAGGTGC
CGCTGCTGATGACGCTGTTGCAGCGCGGCCGCGTGGCAGGGGCCGCCGATGCCGGACGCACCCTGGCCAACCTCAACGCG
GCCGACTATCTGGGCGCGTTGGTCGGCGGGCTGGCCTGGCCATTCCTGCTGCTGCCGCAGTTAGGGATGATCCGCGGTGC
GGCGGTCACCGGCATCGTCAATCTGGCGGCCGCCGGGGTTGTGTCGATCTTCCTGCTGCGCCACGTCGTGTCCGGCCGGC
AACTGGTGACCGCCTTATGCGCGCTCGCCGCGGCGCTCGGGCTGATCGCCACACTGCTGGTGCATTCCCACGACATTGAG
ACCACCGGCCGCCAACAGCTCTACGCCGACCCGATCATCGCCTACCGACACAGCGCCTACCAGGAAATCGTGGTCACCCG
CCGCGGCGATGACCTGCGCCTCTACCTGGACGGAGGTTTGCAGTTCTGCACCCGCGACGAATACCGCTACACCGAAAGCC
TGGTCTACCCGGCAGTCTCCGATGGCGCGCGTTCGGTGCTGGTGCTCGGTGGCGGCGACGGACTGGCAGCCCGCGAACTG
CTGCGCCAACCCGGCATCGAGCAGATCGTGCAGGTGGAACTCGACCCCGCGGTCATCGAACTGGCGCGCACCACCCTGCG
CGACGTCAACGCCGGTTCGCTGGACAACCCGCGCGTACACGTCGTGATCGACGACGCCATGAGCTGGCTACGCGGCGCCG
CGGTCCCCCCGGCTGGCTTCGACGCAGTGATCGTCGACCTTCGCGACCCCGATACTCCCGTGCTGGGTCGGCTGTATTCC
ACCGAGTTCTACGCACTCGCCGCCCGCGCGCTCGCGCCCGGCGGGCTCATGGTCGTGCAGGCAGGCAGCCCGTATTCGAC
CCCGACTGCGTTCTGGCGCATCATCTCCACGATCCGGTCCGCCGGGTATGCCGTCACGCCCTACCACGTGCACGTGCCCA
CCTTCGGCGACTGGGGATTCGCCCTGGCACGCCTTACAGACATCGCGCCCACCCCCGCTGTGCCGAGCACTGCCCCTGCA
CTGCGCTTCCTGGACCAACAGGTGCTCGAGGCCGCGACCGTGTTTTCCGGCGACATCCGGCCCCGCACGTTGGACCCGTC
GACCCTGGACAATCCGCACATTGTTGAGGACATGCGGCACGGCTGGGACTAG

Upstream 100 bases:

>100_bases
GATTCCGTGAGCACATCGACGCACCTGCGCTGCATCCGGCGGTGTTCGCTACCGCCGTCATGCTGCTGGCGGTAGCGGGG
GTAATCGCCGCCGCGTTGTC

Downstream 100 bases:

>100_bases
CGCACCCATCTAGGGCGGCCAGGGTTTGCACAACGCAGCACGGGTTCCGAACGGAACCGGGGCCCGCTCGTAGCCCGGCC
ATAAAAGCATAAAAACAGTA

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MTSTRQAGEATEASVRWRAVLLAAVAACAACGLVYELALLTLAASLNGGGIVATSLIVAGYIAALGAGALLIKPLLAHAA
IAFIAVEAVLGIIGGLSAAALYAAFAFLDELDGSTLVLAVGTALIGGLVGAEVPLLMTLLQRGRVAGAADAGRTLANLNA
ADYLGALVGGLAWPFLLLPQLGMIRGAAVTGIVNLAAAGVVSIFLLRHVVSGRQLVTALCALAAALGLIATLLVHSHDIE
TTGRQQLYADPIIAYRHSAYQEIVVTRRGDDLRLYLDGGLQFCTRDEYRYTESLVYPAVSDGARSVLVLGGGDGLAAREL
LRQPGIEQIVQVELDPAVIELARTTLRDVNAGSLDNPRVHVVIDDAMSWLRGAAVPPAGFDAVIVDLRDPDTPVLGRLYS
TEFYALAARALAPGGLMVVQAGSPYSTPTAFWRIISTIRSAGYAVTPYHVHVPTFGDWGFALARLTDIAPTPAVPSTAPA
LRFLDQQVLEAATVFSGDIRPRTLDPSTLDNPHIVEDMRHGWD

Sequences:

>Translated_523_residues
MTSTRQAGEATEASVRWRAVLLAAVAACAACGLVYELALLTLAASLNGGGIVATSLIVAGYIAALGAGALLIKPLLAHAA
IAFIAVEAVLGIIGGLSAAALYAAFAFLDELDGSTLVLAVGTALIGGLVGAEVPLLMTLLQRGRVAGAADAGRTLANLNA
ADYLGALVGGLAWPFLLLPQLGMIRGAAVTGIVNLAAAGVVSIFLLRHVVSGRQLVTALCALAAALGLIATLLVHSHDIE
TTGRQQLYADPIIAYRHSAYQEIVVTRRGDDLRLYLDGGLQFCTRDEYRYTESLVYPAVSDGARSVLVLGGGDGLAAREL
LRQPGIEQIVQVELDPAVIELARTTLRDVNAGSLDNPRVHVVIDDAMSWLRGAAVPPAGFDAVIVDLRDPDTPVLGRLYS
TEFYALAARALAPGGLMVVQAGSPYSTPTAFWRIISTIRSAGYAVTPYHVHVPTFGDWGFALARLTDIAPTPAVPSTAPA
LRFLDQQVLEAATVFSGDIRPRTLDPSTLDNPHIVEDMRHGWD
>Mature_522_residues
TSTRQAGEATEASVRWRAVLLAAVAACAACGLVYELALLTLAASLNGGGIVATSLIVAGYIAALGAGALLIKPLLAHAAI
AFIAVEAVLGIIGGLSAAALYAAFAFLDELDGSTLVLAVGTALIGGLVGAEVPLLMTLLQRGRVAGAADAGRTLANLNAA
DYLGALVGGLAWPFLLLPQLGMIRGAAVTGIVNLAAAGVVSIFLLRHVVSGRQLVTALCALAAALGLIATLLVHSHDIET
TGRQQLYADPIIAYRHSAYQEIVVTRRGDDLRLYLDGGLQFCTRDEYRYTESLVYPAVSDGARSVLVLGGGDGLAARELL
RQPGIEQIVQVELDPAVIELARTTLRDVNAGSLDNPRVHVVIDDAMSWLRGAAVPPAGFDAVIVDLRDPDTPVLGRLYST
EFYALAARALAPGGLMVVQAGSPYSTPTAFWRIISTIRSAGYAVTPYHVHVPTFGDWGFALARLTDIAPTPAVPSTAPAL
RFLDQQVLEAATVFSGDIRPRTLDPSTLDNPHIVEDMRHGWD

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG4262

COG function: function code R; Predicted spermidine synthase with an N-terminal membrane domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=264, Percent_Identity=26.5151515151515, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1786312, Length=271, Percent_Identity=30.2583025830258, Blast_Score=113, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI212645616, Length=215, Percent_Identity=29.3023255813954, Blast_Score=70, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25154106, Length=263, Percent_Identity=28.8973384030418, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323175, Length=248, Percent_Identity=26.6129032258064, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6325326, Length=269, Percent_Identity=28.2527881040892, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24645443, Length=268, Percent_Identity=29.4776119402985, Blast_Score=93, Evalue=5e-19,
Organism=Drosophila melanogaster, GI21355941, Length=208, Percent_Identity=29.8076923076923, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_MYCBO (Q7TY95)

Other databases:

- EMBL:   BX248343
- RefSeq:   NP_856278.1
- ProteinModelPortal:   Q7TY95
- SMR:   Q7TY95
- EnsemblBacteria:   EBMYCT00000016148
- GeneID:   1091993
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2632
- GeneTree:   EBGT00050000018071
- HOGENOM:   HBG564037
- OMA:   YAKATRF
- ProtClustDB:   PRK03612
- BioCyc:   MBOV233413:MB2632-MONOMER
- BRENDA:   2.5.1.16
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 54684; Mature: 54553

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 261-261 BINDING 313-313 BINDING 333-333 BINDING 386-386 BINDING 389-389

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSTRQAGEATEASVRWRAVLLAAVAACAACGLVYELALLTLAASLNGGGIVATSLIVAG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YIAALGAGALLIKPLLAHAAIAFIAVEAVLGIIGGLSAAALYAAFAFLDELDGSTLVLAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
GTALIGGLVGAEVPLLMTLLQRGRVAGAADAGRTLANLNAADYLGALVGGLAWPFLLLPQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGMIRGAAVTGIVNLAAAGVVSIFLLRHVVSGRQLVTALCALAAALGLIATLLVHSHDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTGRQQLYADPIIAYRHSAYQEIVVTRRGDDLRLYLDGGLQFCTRDEYRYTESLVYPAVS
CCCCHHHHCCHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCC
DGARSVLVLGGGDGLAARELLRQPGIEQIVQVELDPAVIELARTTLRDVNAGSLDNPRVH
CCCCEEEEEECCCCHHHHHHHHCCCHHHHHEECCCHHHHHHHHHHHHCCCCCCCCCCEEE
VVIDDAMSWLRGAAVPPAGFDAVIVDLRDPDTPVLGRLYSTEFYALAARALAPGGLMVVQ
EEEHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AGSPYSTPTAFWRIISTIRSAGYAVTPYHVHVPTFGDWGFALARLTDIAPTPAVPSTAPA
CCCCCCCHHHHHHHHHHHHHCCCEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHH
LRFLDQQVLEAATVFSGDIRPRTLDPSTLDNPHIVEDMRHGWD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
TSTRQAGEATEASVRWRAVLLAAVAACAACGLVYELALLTLAASLNGGGIVATSLIVAG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
YIAALGAGALLIKPLLAHAAIAFIAVEAVLGIIGGLSAAALYAAFAFLDELDGSTLVLAV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHH
GTALIGGLVGAEVPLLMTLLQRGRVAGAADAGRTLANLNAADYLGALVGGLAWPFLLLPQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHH
LGMIRGAAVTGIVNLAAAGVVSIFLLRHVVSGRQLVTALCALAAALGLIATLLVHSHDIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TTGRQQLYADPIIAYRHSAYQEIVVTRRGDDLRLYLDGGLQFCTRDEYRYTESLVYPAVS
CCCCHHHHCCHHHHHHHHHHHHHEEEECCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCC
DGARSVLVLGGGDGLAARELLRQPGIEQIVQVELDPAVIELARTTLRDVNAGSLDNPRVH
CCCCEEEEEECCCCHHHHHHHHCCCHHHHHEECCCHHHHHHHHHHHHCCCCCCCCCCEEE
VVIDDAMSWLRGAAVPPAGFDAVIVDLRDPDTPVLGRLYSTEFYALAARALAPGGLMVVQ
EEEHHHHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEE
AGSPYSTPTAFWRIISTIRSAGYAVTPYHVHVPTFGDWGFALARLTDIAPTPAVPSTAPA
CCCCCCCHHHHHHHHHHHHHCCCEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCCCHHH
LRFLDQQVLEAATVFSGDIRPRTLDPSTLDNPHIVEDMRHGWD
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972