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The map label for this gene is pepE

Identifier: 148823303

GI number: 148823303

Start: 2362168

End: 2363295

Strand: Reverse

Name: pepE

Synonym: TBFG_12124

Alternate gene names: 148823303

Gene position: 2363295-2362168 (Counterclockwise)

Preceding gene: 148823305

Following gene: 148823297

Centisome position: 53.41

GC content: 66.31

Gene sequence:

>1128_bases
ATGGGTTCTCGCCGATTCGACGCCGAGGTTTATGCACGGCGGCTGGCTTTAGCGGCGGCCGCGACGGCGGACGCCGGTCT
GGCGGGTCTGGTGATAACTCCCGGCTACGACCTGTGTTACCTCATCGGGTCGCGAGCGGAGACGTTCGAGCGGCTCACCG
CGTTGGTGTTGCCGGCCGCCGGTGCGCCGGCGGTTGTGCTGCCGCGGCTGGAGCTCGCCGCCCTCAAGCAATCCGCCGCA
GCGGAATTGGGTCTGCGCGTGTGCGATTGGGTCGACGGTGACGACCCCTACGGGTTGGTGAGTGCCGTGTTGGGCGGAGC
TCCGGTAGCGACCGCGGTCACCGATTCCATGCCGGCGTTGCACATGTTGCCGCTGGCCGACGCACTGGGTGTGCTGCCGG
TATTGGCCACCGACGTGCTGCGCAGGCTGCGGATGGTCAAGGAGGAAACCGAGATCGACGCGCTGCGTAAGGCCGGCGCG
GCGATCGATCGAGTGCATGCCCGAGTGCCGGAGTTTCTGGTCCCGGGCCGAACGGAAGCCGACGTAGCCGCCGACATCGC
CGAAGCAATTGTCGCCGAAGGGCATTCGGAGGTAGCGTTCGTCATCGTGGGTTCCGGGCCGCACGGCGCCGACCCGCATC
ACGGATATTCGGACCGCGAATTGCGGGAGGGTGACATCGTTGTCGTCGACATCGGCGGCACGTATGGGCCTGGATACCAC
TCCGACTCCACCCGAACCTACAGCATCGGCGAGCCTGATTCTGATGTAGCGCAGTCATATTCGATGTTGCAGCGAGCCCA
GCGGGCGGCGTTCGAGGCCATCCGCCCAGGGGTGACAGCGGAGCAGGTGGACGCCGCCGCGCGTGACGTGCTCGCCGAGG
CCGGGCTCGCGGAGTATTTTGTGCACCGCACCGGGCACGGCATCGGGCTGTGCGTGCACGAGGAGCCCTATATCGTCGCC
GGCAATGACCTGGTGTTGGTTCCCGGCATGGCGTTTTCCATCGAGCCGGGAATCTATTTCCCGGGCCGGTGGGGCGCCCG
CATCGAGGACATCGTGATCGTGACCGAGGACGGTGCTGTGTCTGTCAACAACTGCCCGCACGAGTTGATCGTGGTGCCGG
TGTCCTAG

Upstream 100 bases:

>100_bases
GCGGCGGAGCCGGGTGCGGCGGGTCGCCACGCATCGGATCGGGTGCGGGCATGCGGGCTATCTTGCCAGCGCTCGCGAAC
GCGCGGTTAACCTGGCTGAC

Downstream 100 bases:

>100_bases
GCCAATGGTGTCGCGCTCAGCCGGGTATCTTGGCGAGGATGTAGTTGGTGATGCCGATGACCGCCTGCTGGCCGCGGCTG
GTGTCGGGCGTCATGATGTC

Product: dipeptidase pepE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MGSRRFDAEVYARRLALAAAATADAGLAGLVITPGYDLCYLIGSRAETFERLTALVLPAAGAPAVVLPRLELAALKQSAA
AELGLRVCDWVDGDDPYGLVSAVLGGAPVATAVTDSMPALHMLPLADALGVLPVLATDVLRRLRMVKEETEIDALRKAGA
AIDRVHARVPEFLVPGRTEADVAADIAEAIVAEGHSEVAFVIVGSGPHGADPHHGYSDRELREGDIVVVDIGGTYGPGYH
SDSTRTYSIGEPDSDVAQSYSMLQRAQRAAFEAIRPGVTAEQVDAAARDVLAEAGLAEYFVHRTGHGIGLCVHEEPYIVA
GNDLVLVPGMAFSIEPGIYFPGRWGARIEDIVIVTEDGAVSVNNCPHELIVVPVS

Sequences:

>Translated_375_residues
MGSRRFDAEVYARRLALAAAATADAGLAGLVITPGYDLCYLIGSRAETFERLTALVLPAAGAPAVVLPRLELAALKQSAA
AELGLRVCDWVDGDDPYGLVSAVLGGAPVATAVTDSMPALHMLPLADALGVLPVLATDVLRRLRMVKEETEIDALRKAGA
AIDRVHARVPEFLVPGRTEADVAADIAEAIVAEGHSEVAFVIVGSGPHGADPHHGYSDRELREGDIVVVDIGGTYGPGYH
SDSTRTYSIGEPDSDVAQSYSMLQRAQRAAFEAIRPGVTAEQVDAAARDVLAEAGLAEYFVHRTGHGIGLCVHEEPYIVA
GNDLVLVPGMAFSIEPGIYFPGRWGARIEDIVIVTEDGAVSVNNCPHELIVVPVS
>Mature_374_residues
GSRRFDAEVYARRLALAAAATADAGLAGLVITPGYDLCYLIGSRAETFERLTALVLPAAGAPAVVLPRLELAALKQSAAA
ELGLRVCDWVDGDDPYGLVSAVLGGAPVATAVTDSMPALHMLPLADALGVLPVLATDVLRRLRMVKEETEIDALRKAGAA
IDRVHARVPEFLVPGRTEADVAADIAEAIVAEGHSEVAFVIVGSGPHGADPHHGYSDRELREGDIVVVDIGGTYGPGYHS
DSTRTYSIGEPDSDVAQSYSMLQRAQRAAFEAIRPGVTAEQVDAAARDVLAEAGLAEYFVHRTGHGIGLCVHEEPYIVAG
NDLVLVPGMAFSIEPGIYFPGRWGARIEDIVIVTEDGAVSVNNCPHELIVVPVS

Specific function: Unknown

COG id: COG0006

COG function: function code E; Xaa-Pro aminopeptidase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M24B family

Homologues:

Organism=Homo sapiens, GI93141226, Length=238, Percent_Identity=31.9327731092437, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI11559925, Length=262, Percent_Identity=26.7175572519084, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI264681563, Length=227, Percent_Identity=29.5154185022026, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI260593665, Length=280, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI149589008, Length=280, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788728, Length=236, Percent_Identity=40.2542372881356, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI1789275, Length=264, Percent_Identity=28.7878787878788, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1786364, Length=210, Percent_Identity=27.1428571428571, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI21357079, Length=286, Percent_Identity=26.5734265734266, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PEPE_MYCBO (P65811)

Other databases:

- EMBL:   BX248341
- RefSeq:   NP_855765.1
- ProteinModelPortal:   P65811
- SMR:   P65811
- MEROPS:   M24.034
- EnsemblBacteria:   EBMYCT00000018110
- GeneID:   1091134
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb2116c
- GeneTree:   EBGT00050000016752
- HOGENOM:   HBG545079
- OMA:   VEYIAPA
- ProtClustDB:   CLSK872002
- BioCyc:   MBOV233413:MB2116C-MONOMER
- GO:   GO:0006508
- InterPro:   IPR000587
- InterPro:   IPR000994
- InterPro:   IPR001131
- Gene3D:   G3DSA:3.90.230.10
- PANTHER:   PTHR10804

Pfam domain/function: PF01321 Creatinase_N; PF00557 Peptidase_M24; SSF55920 Peptidase_M24_cat_core

EC number: 3.4.-.- [C]

Molecular weight: Translated: 39440; Mature: 39309

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS00491 PROLINE_PEPTIDASE

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x187cdfa0)-; HASH(0x1b286904)-;

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSRRFDAEVYARRLALAAAATADAGLAGLVITPGYDLCYLIGSRAETFERLTALVLPAA
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHCCCC
GAPAVVLPRLELAALKQSAAAELGLRVCDWVDGDDPYGLVSAVLGGAPVATAVTDSMPAL
CCCEEEECCHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCH
HMLPLADALGVLPVLATDVLRRLRMVKEETEIDALRKAGAAIDRVHARVPEFLVPGRTEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCH
DVAADIAEAIVAEGHSEVAFVIVGSGPHGADPHHGYSDRELREGDIVVVDIGGTYGPGYH
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
SDSTRTYSIGEPDSDVAQSYSMLQRAQRAAFEAIRPGVTAEQVDAAARDVLAEAGLAEYF
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
VHRTGHGIGLCVHEEPYIVAGNDLVLVPGMAFSIEPGIYFPGRWGARIEDIVIVTEDGAV
HHHCCCCEEEEEECCCEEEECCCEEEECCEEEEECCCEECCCCCCCCEEEEEEEECCCEE
SVNNCPHELIVVPVS
EECCCCCEEEEEECH
>Mature Secondary Structure 
GSRRFDAEVYARRLALAAAATADAGLAGLVITPGYDLCYLIGSRAETFERLTALVLPAA
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEECCHHHHHHHHHHHHCCCC
GAPAVVLPRLELAALKQSAAAELGLRVCDWVDGDDPYGLVSAVLGGAPVATAVTDSMPAL
CCCEEEECCHHHHHHHHHHHHHHCCHHCCCCCCCCCHHHHHHHHCCCCHHHHHHCCCCCH
HMLPLADALGVLPVLATDVLRRLRMVKEETEIDALRKAGAAIDRVHARVPEFLVPGRTEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCH
DVAADIAEAIVAEGHSEVAFVIVGSGPHGADPHHGYSDRELREGDIVVVDIGGTYGPGYH
HHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCC
SDSTRTYSIGEPDSDVAQSYSMLQRAQRAAFEAIRPGVTAEQVDAAARDVLAEAGLAEYF
CCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHH
VHRTGHGIGLCVHEEPYIVAGNDLVLVPGMAFSIEPGIYFPGRWGARIEDIVIVTEDGAV
HHHCCCCEEEEEECCCEEEECCCEEEECCEEEEECCCEECCCCCCCCEEEEEEEECCCEE
SVNNCPHELIVVPVS
EECCCCCEEEEEECH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972