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The map label for this gene is cobK

Identifier: 148823285

GI number: 148823285

Start: 2342103

End: 2342837

Strand: Reverse

Name: cobK

Synonym: TBFG_12106

Alternate gene names: 148823285

Gene position: 2342837-2342103 (Counterclockwise)

Preceding gene: 148823286

Following gene: 148823284

Centisome position: 52.95

GC content: 66.67

Gene sequence:

>735_bases
GTGACCCGGGTGTTGTTGCTCGGCGGCACCGCCGAGGGCCGTGCGCTGGCGAAAGAGTTGCACCCACACGTTGAGATCGT
CAGCTCGCTGGCCGGCCGGGTACCCAACCCTGCCCTGCCGATCGGTCCGGTGCGCATCGGCGGGTTCGGCGGTGTCGAGG
GGCTGCGCGGTTGGCTGCGAGAGGAGCGCATCGATGCCGTCGTCGACGCCACCCACCCCTTCGCGGTAACCATCACCGCG
CACGCCGCGCAGGTGTGCGGTGAGCTCGGGCTCCCGTACCTGGTACTGGCTCGCCCGCCGTGGGATCCCGGTACCGCCAT
CATCGCGGTATCGGACATCGAGGCTGCAGACGTTGTTGCTGAACAAGGTTATTCGCGAGTGTTCCTGACCACCGGACGCT
CGGGTATTGCGGCCTTCGCCAACAGCGACGCGTGGTTTTTGATCCGCGTGGTTACCGCGCCCGATGGCACCGCCCTGCCG
CGGCGGCACAAACTGGTGCTATCTCGCGGGCCATATGGTTACCACGATGAGTTCGCACTGCTGCGTGAGCAGCGCATCGA
CGCATTGGTCACCAAGAACAGCGGTGGCAAGATGACCCGAGCGAAGCTGGATGCCGCTGCTGCGCTGGGTATTTCGGTGG
TCATGATAGCGCGCCCGCTGCTGCCGGCTGGGGTAGCGGCGGTCGATTCTGTCCACCGGGCCGCCATGTGGGTGGCCGGT
CTGCCTAGCCGGTGA

Upstream 100 bases:

>100_bases
TCACCAGGACCGCCGTGATCGTTGTTGGCGACGTGCTGACCGCTGAGGGCTTTACCGACAGCTACCTATATTCGGTGGCA
CGGCACGGTCGGTATGCACA

Downstream 100 bases:

>100_bases
GGTCGTCGGGCTCCGCGTCGGCAAGCAGCGCATCGCGCGCTCGAGCGACACGGGATCGGATGGTGCCCACCGGGCAGCCG
CACACCGCCGCGGCGTCCGC

Product: cobalt-precorrin-6x reductase

Products: NA

Alternate protein names: Precorrin-6X reductase

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTRVLLLGGTAEGRALAKELHPHVEIVSSLAGRVPNPALPIGPVRIGGFGGVEGLRGWLREERIDAVVDATHPFAVTITA
HAAQVCGELGLPYLVLARPPWDPGTAIIAVSDIEAADVVAEQGYSRVFLTTGRSGIAAFANSDAWFLIRVVTAPDGTALP
RRHKLVLSRGPYGYHDEFALLREQRIDALVTKNSGGKMTRAKLDAAAALGISVVMIARPLLPAGVAAVDSVHRAAMWVAG
LPSR

Sequences:

>Translated_244_residues
MTRVLLLGGTAEGRALAKELHPHVEIVSSLAGRVPNPALPIGPVRIGGFGGVEGLRGWLREERIDAVVDATHPFAVTITA
HAAQVCGELGLPYLVLARPPWDPGTAIIAVSDIEAADVVAEQGYSRVFLTTGRSGIAAFANSDAWFLIRVVTAPDGTALP
RRHKLVLSRGPYGYHDEFALLREQRIDALVTKNSGGKMTRAKLDAAAALGISVVMIARPLLPAGVAAVDSVHRAAMWVAG
LPSR
>Mature_243_residues
TRVLLLGGTAEGRALAKELHPHVEIVSSLAGRVPNPALPIGPVRIGGFGGVEGLRGWLREERIDAVVDATHPFAVTITAH
AAQVCGELGLPYLVLARPPWDPGTAIIAVSDIEAADVVAEQGYSRVFLTTGRSGIAAFANSDAWFLIRVVTAPDGTALPR
RHKLVLSRGPYGYHDEFALLREQRIDALVTKNSGGKMTRAKLDAAAALGISVVMIARPLLPAGVAAVDSVHRAAMWVAGL
PSR

Specific function: Catalyzes the reduction of the macrocycle of precorrin- 6X into precorrin-6Y

COG id: COG2099

COG function: function code H; Precorrin-6x reductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin-6x reductase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBK_MYCTU (Q10680)

Other databases:

- EMBL:   BX842578
- EMBL:   AE000516
- PIR:   A70765
- RefSeq:   NP_216586.1
- RefSeq:   NP_336596.1
- EnsemblBacteria:   EBMYCT00000002866
- EnsemblBacteria:   EBMYCT00000069188
- GeneID:   887566
- GeneID:   924600
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2130
- KEGG:   mtu:Rv2070c
- TIGR:   MT2130
- TubercuList:   Rv2070c
- GeneTree:   EBGT00050000016507
- HOGENOM:   HBG711334
- OMA:   NERCEVI
- ProtClustDB:   PRK08057
- BRENDA:   1.3.1.54
- InterPro:   IPR003723
- TIGRFAMs:   TIGR00715

Pfam domain/function: PF02571 CbiJ

EC number: =1.3.1.54

Molecular weight: Translated: 25737; Mature: 25606

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS51014 COBK_CBIJ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRVLLLGGTAEGRALAKELHPHVEIVSSLAGRVPNPALPIGPVRIGGFGGVEGLRGWLR
CCEEEEEECCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHH
EERIDAVVDATHPFAVTITAHAAQVCGELGLPYLVLARPPWDPGTAIIAVSDIEAADVVA
HHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCHHHHHH
EQGYSRVFLTTGRSGIAAFANSDAWFLIRVVTAPDGTALPRRHKLVLSRGPYGYHDEFAL
HCCCCEEEEEECCCCEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHH
LREQRIDALVTKNSGGKMTRAKLDAAAALGISVVMIARPLLPAGVAAVDSVHRAAMWVAG
HHHHHHHEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
LPSR
CCCC
>Mature Secondary Structure 
TRVLLLGGTAEGRALAKELHPHVEIVSSLAGRVPNPALPIGPVRIGGFGGVEGLRGWLR
CEEEEEECCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCEEECCCCCHHHHHHHHH
EERIDAVVDATHPFAVTITAHAAQVCGELGLPYLVLARPPWDPGTAIIAVSDIEAADVVA
HHHHHHHHCCCCCEEEEEEHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECCCHHHHHH
EQGYSRVFLTTGRSGIAAFANSDAWFLIRVVTAPDGTALPRRHKLVLSRGPYGYHDEFAL
HCCCCEEEEEECCCCEEEEECCCCEEEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHH
LREQRIDALVTKNSGGKMTRAKLDAAAALGISVVMIARPLLPAGVAAVDSVHRAAMWVAG
HHHHHHHEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
LPSR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036