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The map label for this gene is pcnA [H]

Identifier: 148663774

GI number: 148663774

Start: 4400077

End: 4401519

Strand: Reverse

Name: pcnA [H]

Synonym: MRA_3946

Alternate gene names: 148663774

Gene position: 4401519-4400077 (Counterclockwise)

Preceding gene: 148663783

Following gene: 148663773

Centisome position: 99.58

GC content: 66.53

Gene sequence:

>1443_bases
GTGCCGGAAGCCGTCCAGGAAGCCGATCTGCTAACCGCCGCTGCGGTTGCCTTGAACAGGCATGCTGCCTTATTGCGGGA
ACTCGGGTCGGTGTTCGCCGCCGCGGGACACGAGTTGTATCTGGTCGGCGGTTCGGTGCGAGATGCACTGTTGGGCCGGT
TGAGCCCCGACCTGGACTTCACCACCGACGCCCGTCCCGAGCGGGTGCAGGAGATCGTGCGGCCGTGGGCCGATGCGGTG
TGGGATACCGGAATCGAATTCGGCACCGTCGGCGTGGGTAAGAGCGACCACCGCATGGAGATCACCACATTCCGTGCCGA
CAGCTACGACCGGGTTTCGCGTCATCCAGAGGTACGTTTCGGCGATTGCCTCGAGGGCGATCTGGTCCGCCGCGACTTCA
CCACGAACGCAATGGCTGTGCGCGTCACCGCCACTGGGCCGGGCGAATTCCTGGATCCGCTTGGTGGCTTGGCGGCGCTG
CGGGCCAAGGTGTTAGACACCCCGGCGGCGCCGTCGGGGTCCTTTGGCGACGATCCGTTGCGGATGCTGCGCGCCGCGCG
GTTCGTCTCGCAACTTGGATTCGCGGTGGCGCCGCGGGTGCGCGCGGCGATCGAAGAGATGGCGCCGCAGTTGGCCCGAA
TCAGCGCCGAACGGGTGGCCGCCGAGCTGGACAAGCTGCTGGTCGGTGAGGATCCGGCCGCGGGTATCGACCTGATGGTG
CAGAGCGGTATGGGTGCTGTGGTCTTGCCTGAAATCGGTGGGATGCGGATGGCGATCGACGAACATCACCAGCACAAGGA
CGTCTATCAGCATTCCTTGACCGTGCTGCGGCAGGCGATCGCGCTGGAGGACGACGGCCCGGATCTGGTGTTGCGCTGGG
CGGCGCTGCTGCACGACATCGGCAAGCCCGCCACCCGCCGTCACGAACCCGACGGTGGGGTGAGCTTCCATCACCACGAA
GTGGTCGGCGCCAAGATGGTGCGCAAGCGGATGCGGGCGCTGAAGTATTCCAAGCAGATGATCGACGACATCTCGCAGCT
GGTCTACCTGCATCTGCGGTTTCACGGCTACGGCGATGGGAAATGGACCGACTCTGCGGTGCGCCGCTATGTCACCGACG
CCGGGGCCCTACTGCCACGGCTGCACAAGCTGGTGCGCGCCGACTGCACGACCCGCAACAAGCGCCGGGCCGCGCGGTTG
CAGGCCAGTTACGACCGGCTGGAAGAGCGGATCGCGGAGCTGGCCGCCCAGGAGGATCTGGATCGGGTGCGCCCCGACCT
GGACGGCAACCAGATCATGGCGGTGCTCGACATTCCGGCGGGCCCGCAAGTCGGCGAGGCGTGGCGCTACTTGAAGGAGC
TGCGGCTAGAGCGCGGCCCGTTGTCCACCGAGGAGGCGACAACCGAGCTGCTGTCCTGGTGGAAATCACGGGGGAACCGC
TAG

Upstream 100 bases:

>100_bases
CGTCCCGACACCGGCCAATGCGACGGGTGCGGCAACGAGGGTTGCCGGTTCCTCCACCGACCGTCCCGGCCCACTTCGAC
TGCCATTAGGCTGATCGAAC

Downstream 100 bases:

>100_bases
CTTGGGAGTCGCGTCAGAACGGTTGTGGAGTACTGCATAGCCGGCGACGACGGCAGCGCCGGGATCTGGAACCGCCCGTT
CGACGTCGACCTCGACGGTG

Product: polyA polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPEAVQEADLLTAAAVALNRHAALLRELGSVFAAAGHELYLVGGSVRDALLGRLSPDLDFTTDARPERVQEIVRPWADAV
WDTGIEFGTVGVGKSDHRMEITTFRADSYDRVSRHPEVRFGDCLEGDLVRRDFTTNAMAVRVTATGPGEFLDPLGGLAAL
RAKVLDTPAAPSGSFGDDPLRMLRAARFVSQLGFAVAPRVRAAIEEMAPQLARISAERVAAELDKLLVGEDPAAGIDLMV
QSGMGAVVLPEIGGMRMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDIGKPATRRHEPDGGVSFHHHE
VVGAKMVRKRMRALKYSKQMIDDISQLVYLHLRFHGYGDGKWTDSAVRRYVTDAGALLPRLHKLVRADCTTRNKRRAARL
QASYDRLEERIAELAAQEDLDRVRPDLDGNQIMAVLDIPAGPQVGEAWRYLKELRLERGPLSTEEATTELLSWWKSRGNR

Sequences:

>Translated_480_residues
MPEAVQEADLLTAAAVALNRHAALLRELGSVFAAAGHELYLVGGSVRDALLGRLSPDLDFTTDARPERVQEIVRPWADAV
WDTGIEFGTVGVGKSDHRMEITTFRADSYDRVSRHPEVRFGDCLEGDLVRRDFTTNAMAVRVTATGPGEFLDPLGGLAAL
RAKVLDTPAAPSGSFGDDPLRMLRAARFVSQLGFAVAPRVRAAIEEMAPQLARISAERVAAELDKLLVGEDPAAGIDLMV
QSGMGAVVLPEIGGMRMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDIGKPATRRHEPDGGVSFHHHE
VVGAKMVRKRMRALKYSKQMIDDISQLVYLHLRFHGYGDGKWTDSAVRRYVTDAGALLPRLHKLVRADCTTRNKRRAARL
QASYDRLEERIAELAAQEDLDRVRPDLDGNQIMAVLDIPAGPQVGEAWRYLKELRLERGPLSTEEATTELLSWWKSRGNR
>Mature_479_residues
PEAVQEADLLTAAAVALNRHAALLRELGSVFAAAGHELYLVGGSVRDALLGRLSPDLDFTTDARPERVQEIVRPWADAVW
DTGIEFGTVGVGKSDHRMEITTFRADSYDRVSRHPEVRFGDCLEGDLVRRDFTTNAMAVRVTATGPGEFLDPLGGLAALR
AKVLDTPAAPSGSFGDDPLRMLRAARFVSQLGFAVAPRVRAAIEEMAPQLARISAERVAAELDKLLVGEDPAAGIDLMVQ
SGMGAVVLPEIGGMRMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDIGKPATRRHEPDGGVSFHHHEV
VGAKMVRKRMRALKYSKQMIDDISQLVYLHLRFHGYGDGKWTDSAVRRYVTDAGALLPRLHKLVRADCTTRNKRRAARLQ
ASYDRLEERIAELAAQEDLDRVRPDLDGNQIMAVLDIPAGPQVGEAWRYLKELRLERGPLSTEEATTELLSWWKSRGNR

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=274, Percent_Identity=30.2919708029197, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789436, Length=324, Percent_Identity=30.8641975308642, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87081691, Length=231, Percent_Identity=31.1688311688312, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71995920, Length=209, Percent_Identity=29.6650717703349, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6321016, Length=242, Percent_Identity=30.5785123966942, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI281360112, Length=215, Percent_Identity=30.6976744186047, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI21357337, Length=215, Percent_Identity=30.6976744186047, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 53088; Mature: 52957

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00018 EF_HAND_1 ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEAVQEADLLTAAAVALNRHAALLRELGSVFAAAGHELYLVGGSVRDALLGRLSPDLDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCC
TTDARPERVQEIVRPWADAVWDTGIEFGTVGVGKSDHRMEITTFRADSYDRVSRHPEVRF
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEECCCHHHHHCCCCCCC
GDCLEGDLVRRDFTTNAMAVRVTATGPGEFLDPLGGLAALRAKVLDTPAAPSGSFGDDPL
CCCCCCCHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RMLRAARFVSQLGFAVAPRVRAAIEEMAPQLARISAERVAAELDKLLVGEDPAAGIDLMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QSGMGAVVLPEIGGMRMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDI
HCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GKPATRRHEPDGGVSFHHHEVVGAKMVRKRMRALKYSKQMIDDISQLVYLHLRFHGYGDG
CCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
KWTDSAVRRYVTDAGALLPRLHKLVRADCTTRNKRRAARLQASYDRLEERIAELAAQEDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRVRPDLDGNQIMAVLDIPAGPQVGEAWRYLKELRLERGPLSTEEATTELLSWWKSRGNR
HHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PEAVQEADLLTAAAVALNRHAALLRELGSVFAAAGHELYLVGGSVRDALLGRLSPDLDF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCC
TTDARPERVQEIVRPWADAVWDTGIEFGTVGVGKSDHRMEITTFRADSYDRVSRHPEVRF
CCCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCEEEEEEEECCCHHHHHCCCCCCC
GDCLEGDLVRRDFTTNAMAVRVTATGPGEFLDPLGGLAALRAKVLDTPAAPSGSFGDDPL
CCCCCCCHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RMLRAARFVSQLGFAVAPRVRAAIEEMAPQLARISAERVAAELDKLLVGEDPAAGIDLMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
QSGMGAVVLPEIGGMRMAIDEHHQHKDVYQHSLTVLRQAIALEDDGPDLVLRWAALLHDI
HCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GKPATRRHEPDGGVSFHHHEVVGAKMVRKRMRALKYSKQMIDDISQLVYLHLRFHGYGDG
CCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCC
KWTDSAVRRYVTDAGALLPRLHKLVRADCTTRNKRRAARLQASYDRLEERIAELAAQEDL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRVRPDLDGNQIMAVLDIPAGPQVGEAWRYLKELRLERGPLSTEEATTELLSWWKSRGNR
HHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA