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The map label for this gene is nth

Identifier: 148663537

GI number: 148663537

Start: 4123603

End: 4124340

Strand: Reverse

Name: nth

Synonym: MRA_3709

Alternate gene names: 148663537

Gene position: 4124340-4123603 (Counterclockwise)

Preceding gene: 148663540

Following gene: 148663536

Centisome position: 93.31

GC content: 64.91

Gene sequence:

>738_bases
GTGCCCGGGCGCTGGTCTGCAGAAACCCGGCTCGCCTTGGTGCGACGGGCGCGGCGAATGAATCGCGCTTTGGCACAGGC
ATTTCCGCACGTATACTGCGAGCTGGACTTCACCACGCCGCTCGAGCTAGCCGTGGCAACCATACTTTCGGCGCAGAGCA
CCGACAAACGGGTGAATTTGACGACGCCAGCCTTGTTCGCGCGGTATCGGACGGCACGGGACTACGCCCAGGCGGATCGC
ACCGAGCTCGAGAGCCTCATCCGCCCCACCGGCTTCTACCGCAACAAAGCGGCCTCTCTCATCGGCCTGGGGCAGGCCCT
AGTTGAGCGGTTTGGCGGTGAGGTGCCGGCCACCATGGACAAGTTGGTGACGCTGCCCGGGGTGGGGCGCAAAACCGCCA
ATGTCATCCTGGGTAACGCATTCGGTATCCCCGGAATCACGGTGGATACTCATTTCGGACGATTGGTGCGCCGGTGGCGC
TGGACCACCGCAGAGGACCCGGTCAAGGTGGAGCAGGCGGTCGGTGAGCTGATCGAACGCAAGGAGTGGACCTTGCTCAG
CCACCGAGTGATCTTTCACGGACGGCGGGTGTGCCACGCCCGCAGACCGGCGTGTGGTGTTTGTGTGCTTGCCAAAGACT
GCCCCTCCTTCGGGCTTGGCCCCACTGAACCGCTGCTCGCGGCGCCCCTGGTCCAGGGCCCCGAGACCGATCACCTGCTG
GCCCTGGCTGGACTGTAA

Upstream 100 bases:

>100_bases
AATCAACACGAGGTCTCAGTGAGATCACGAAATCAGTACTCTGTCGTAGGTGACAGCGGCGAAGTCATCCAGATCGAAGC
CAGCGGCGCGTGCCGCCGAC

Downstream 100 bases:

>100_bases
TGCCAAGCCTGCCAACAACCCCCGCTGAGACCGCAATGACGACGTTAACCGGAAAGACCCGCTGGACCATCGCGATCCTG
GCGGTGGTGGCAGCGCTGAT

Product: ultraviolet N-glycosylase/AP lyase

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MPGRWSAETRLALVRRARRMNRALAQAFPHVYCELDFTTPLELAVATILSAQSTDKRVNLTTPALFARYRTARDYAQADR
TELESLIRPTGFYRNKAASLIGLGQALVERFGGEVPATMDKLVTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWR
WTTAEDPVKVEQAVGELIERKEWTLLSHRVIFHGRRVCHARRPACGVCVLAKDCPSFGLGPTEPLLAAPLVQGPETDHLL
ALAGL

Sequences:

>Translated_245_residues
MPGRWSAETRLALVRRARRMNRALAQAFPHVYCELDFTTPLELAVATILSAQSTDKRVNLTTPALFARYRTARDYAQADR
TELESLIRPTGFYRNKAASLIGLGQALVERFGGEVPATMDKLVTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWR
WTTAEDPVKVEQAVGELIERKEWTLLSHRVIFHGRRVCHARRPACGVCVLAKDCPSFGLGPTEPLLAAPLVQGPETDHLL
ALAGL
>Mature_244_residues
PGRWSAETRLALVRRARRMNRALAQAFPHVYCELDFTTPLELAVATILSAQSTDKRVNLTTPALFARYRTARDYAQADRT
ELESLIRPTGFYRNKAASLIGLGQALVERFGGEVPATMDKLVTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWRW
TTAEDPVKVEQAVGELIERKEWTLLSHRVIFHGRRVCHARRPACGVCVLAKDCPSFGLGPTEPLLAAPLVQGPETDHLLA
LAGL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family

Homologues:

Organism=Homo sapiens, GI4505471, Length=223, Percent_Identity=29.5964125560538, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=37.0731707317073, Blast_Score=137, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=32.7683615819209, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=192, Percent_Identity=31.25, Blast_Score=84, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6319304, Length=195, Percent_Identity=28.2051282051282, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45550361, Length=175, Percent_Identity=32.5714285714286, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): END3_MYCBO (P63541)

Other databases:

- EMBL:   BX248346
- RefSeq:   NP_857337.1
- ProteinModelPortal:   P63541
- SMR:   P63541
- EnsemblBacteria:   EBMYCT00000017446
- GeneID:   1093265
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3698c
- GeneTree:   EBGT00050000016637
- HOGENOM:   HBG464473
- OMA:   FGEPTIA
- ProtClustDB:   CLSK799395
- BioCyc:   MBOV233413:MB3698C-MONOMER
- BRENDA:   4.2.99.18
- GO:   GO:0005622
- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- Gene3D:   G3DSA:1.10.340.30
- Gene3D:   G3DSA:1.10.1670.10
- SMART:   SM00478
- SMART:   SM00525
- SMART:   SM00278
- TIGRFAMs:   TIGR01083

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD; SSF48150 DNA_glycsylse

EC number: =4.2.99.18

Molecular weight: Translated: 27031; Mature: 26899

Theoretical pI: Translated: 10.26; Mature: 10.26

Prosite motif: PS00764 ENDONUCLEASE_III_1; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPGRWSAETRLALVRRARRMNRALAQAFPHVYCELDFTTPLELAVATILSAQSTDKRVNL
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEC
TTPALFARYRTARDYAQADRTELESLIRPTGFYRNKAASLIGLGQALVERFGGEVPATMD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KLVTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWRWTTAEDPVKVEQAVGELIER
HHHCCCCCCCHHHHEEEECCCCCCCCEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KEWTLLSHRVIFHGRRVCHARRPACGVCVLAKDCPSFGLGPTEPLLAAPLVQGPETDHLL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCEE
ALAGL
EECCC
>Mature Secondary Structure 
PGRWSAETRLALVRRARRMNRALAQAFPHVYCELDFTTPLELAVATILSAQSTDKRVNL
CCCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEC
TTPALFARYRTARDYAQADRTELESLIRPTGFYRNKAASLIGLGQALVERFGGEVPATMD
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHH
KLVTLPGVGRKTANVILGNAFGIPGITVDTHFGRLVRRWRWTTAEDPVKVEQAVGELIER
HHHCCCCCCCHHHHEEEECCCCCCCCEECHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
KEWTLLSHRVIFHGRRVCHARRPACGVCVLAKDCPSFGLGPTEPLLAAPLVQGPETDHLL
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCCCEE
ALAGL
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972