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The map label for this gene is fmu [C]

Identifier: 148661198

GI number: 148661198

Start: 1584411

End: 1585784

Strand: Direct

Name: fmu [C]

Synonym: MRA_1416

Alternate gene names: 148661198

Gene position: 1584411-1585784 (Clockwise)

Preceding gene: 148661197

Following gene: 148661199

Centisome position: 35.85

GC content: 70.96

Gene sequence:

>1374_bases
ATGACCCCTAGATCGCGTGGGCCGCGCCGCCGGCCGCTGGACCCGGCGCGTCGTGCGGCCTTCGAGACGCTGCGGGCGGT
TAGTGCGCGCGACGCCTACGCGAACCTGGTGTTGCCCGCGCTGCTGGCCCAACGCGGTATCGGCGGTCGCGACGCCGCGT
TCGCCACCGAGCTGACATACGGCACCTGCCGAGCCCGCGGCCTGCTCGACGCGGTCATCGGTGCGGCCGCCGAGCGTTCG
CCGCAGGCGATCGATCCGGTGCTGCTAGACCTGTTGCGGCTCGGCACCTACCAATTGCTGCGCACGCGGGTCGACGCACA
CGCCGCAGTGTCGACCACCGTCGAGCAGGCCGGAATCGAATTCGATTCGGCGCGAGCAGGTTTCGTCAACGGTGTACTAC
GAACGATCGCCGGCCGAGACGAGCGGTCCTGGGTTGGCGAACTCGCTCCTGATGCGCAGAACGATCCGATCGGGCATGCC
GCGTTCGTGCATGCGCATCCCCGATGGATCGCCCAGGCCTTTGCTGACGCGTTGGGCGCGGCGGTCGGGGAGCTCGAGGC
AGTTTTGGCCAGCGACGACGAACGGCCAGCGGTGCACCTGGCGGCACGCCCCGGGGTGCTGACCGCCGGCGAACTGGCCC
GCGCGGTGCGCGGAACCGTCGGTCGGTATTCGCCGTTTGCGGTGTATCTGCCGCGCGGTGACCCGGGGCGACTGGCGCCG
GTGCGCGACGGCCAAGCGCTGGTCCAGGACGAGGGCAGCCAGTTAGTCGCCCGAGCATTGACCCTGGCGCCAGTCGACGG
CGATACCGGACGGTGGCTGGACCTGTGTGCCGGACCGGGCGGCAAGACCGCGCTGTTGGCCGGGCTGGGTTTGCAGTGCG
CAGCCCGGGTGACCGCGGTGGAACCCTCGCCACACCGCGCGGACCTGGTAGCACAGAACACCCGCGGGCTGCCGGTTGAG
CTCTTGCGTGTCGACGGGCGGCACACCGACCTCGACCCGGGTTTCGACCGGGTGCTGGTGGATGCGCCCTGCACCGGGCT
GGGCGCGTTACGCCGTCGGCCGGAGGCCCGTTGGCGTCGTCAGCCGGCGGACGTAGCGGCACTGGCCAAGCTACAACGCG
AGTTGTTGAGCGCCGCCATCGCGCTGACTCGGCCCGGCGGTGTCGTGCTCTATGCCACATGCTCGCCGCACCTGGCCGAG
ACTGTGGGTGCTGTCGCCGACGCGCTACGCCGACATCCGGTTCACGCGCTCGATACCCGCCCACTGTTCGAGCCGGTGAT
CGCGGGGCTGGGGGAGGGGCCCCACGTTCAGCTGTGGCCGCACCGGCACGGTACCGACGCCATGTTCGCCGCGGCGTTGC
GCCGCCTGACGTGA

Upstream 100 bases:

>100_bases
AACCGGTGCGGCTGGGCCAGATTCAGCCGCCCGGCAAGAAACTCATGAACGCGGCCGACTGGGCGCGGGGCGCACGGCTG
GACCTGGCCGCACGGGCAAC

Downstream 100 bases:

>100_bases
GGTTCGCCGCAGCGGCTCAGTAATGTGTCGCTCATGGCCGGTAGCACGGGGGGACCGCTGATAGCGCCGTCGATCCTAGC
CGCTGATTTCGCCAGACTCG

Product: Fmu protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 456

Protein sequence:

>457_residues
MTPRSRGPRRRPLDPARRAAFETLRAVSARDAYANLVLPALLAQRGIGGRDAAFATELTYGTCRARGLLDAVIGAAAERS
PQAIDPVLLDLLRLGTYQLLRTRVDAHAAVSTTVEQAGIEFDSARAGFVNGVLRTIAGRDERSWVGELAPDAQNDPIGHA
AFVHAHPRWIAQAFADALGAAVGELEAVLASDDERPAVHLAARPGVLTAGELARAVRGTVGRYSPFAVYLPRGDPGRLAP
VRDGQALVQDEGSQLVARALTLAPVDGDTGRWLDLCAGPGGKTALLAGLGLQCAARVTAVEPSPHRADLVAQNTRGLPVE
LLRVDGRHTDLDPGFDRVLVDAPCTGLGALRRRPEARWRRQPADVAALAKLQRELLSAAIALTRPGGVVLYATCSPHLAE
TVGAVADALRRHPVHALDTRPLFEPVIAGLGEGPHVQLWPHRHGTDAMFAAALRRLT

Sequences:

>Translated_457_residues
MTPRSRGPRRRPLDPARRAAFETLRAVSARDAYANLVLPALLAQRGIGGRDAAFATELTYGTCRARGLLDAVIGAAAERS
PQAIDPVLLDLLRLGTYQLLRTRVDAHAAVSTTVEQAGIEFDSARAGFVNGVLRTIAGRDERSWVGELAPDAQNDPIGHA
AFVHAHPRWIAQAFADALGAAVGELEAVLASDDERPAVHLAARPGVLTAGELARAVRGTVGRYSPFAVYLPRGDPGRLAP
VRDGQALVQDEGSQLVARALTLAPVDGDTGRWLDLCAGPGGKTALLAGLGLQCAARVTAVEPSPHRADLVAQNTRGLPVE
LLRVDGRHTDLDPGFDRVLVDAPCTGLGALRRRPEARWRRQPADVAALAKLQRELLSAAIALTRPGGVVLYATCSPHLAE
TVGAVADALRRHPVHALDTRPLFEPVIAGLGEGPHVQLWPHRHGTDAMFAAALRRLT
>Mature_456_residues
TPRSRGPRRRPLDPARRAAFETLRAVSARDAYANLVLPALLAQRGIGGRDAAFATELTYGTCRARGLLDAVIGAAAERSP
QAIDPVLLDLLRLGTYQLLRTRVDAHAAVSTTVEQAGIEFDSARAGFVNGVLRTIAGRDERSWVGELAPDAQNDPIGHAA
FVHAHPRWIAQAFADALGAAVGELEAVLASDDERPAVHLAARPGVLTAGELARAVRGTVGRYSPFAVYLPRGDPGRLAPV
RDGQALVQDEGSQLVARALTLAPVDGDTGRWLDLCAGPGGKTALLAGLGLQCAARVTAVEPSPHRADLVAQNTRGLPVEL
LRVDGRHTDLDPGFDRVLVDAPCTGLGALRRRPEARWRRQPADVAALAKLQRELLSAAIALTRPGGVVLYATCSPHLAET
VGAVADALRRHPVHALDTRPLFEPVIAGLGEGPHVQLWPHRHGTDAMFAAALRRLT

Specific function: May act as RNA methyltransferase

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family

Homologues:

Organism=Homo sapiens, GI32698918, Length=219, Percent_Identity=31.9634703196347, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI76150625, Length=228, Percent_Identity=29.3859649122807, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI76150623, Length=228, Percent_Identity=29.3859649122807, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI40316918, Length=184, Percent_Identity=37.5, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI2367212, Length=391, Percent_Identity=30.9462915601023, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI87081985, Length=228, Percent_Identity=29.3859649122807, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536757, Length=226, Percent_Identity=29.646017699115, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324268, Length=207, Percent_Identity=28.9855072463768, Blast_Score=84, Evalue=5e-17,
Organism=Drosophila melanogaster, GI22024126, Length=232, Percent_Identity=28.8793103448276, Blast_Score=85, Evalue=9e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y1407_MYCTU (P71675)

Other databases:

- EMBL:   BX842576
- EMBL:   AE000516
- PIR:   D70901
- RefSeq:   NP_215923.1
- RefSeq:   NP_335902.1
- ProteinModelPortal:   P71675
- SMR:   P71675
- EnsemblBacteria:   EBMYCT00000002571
- EnsemblBacteria:   EBMYCT00000069025
- GeneID:   886720
- GeneID:   924521
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT1451
- KEGG:   mtu:Rv1407
- TIGR:   MT1451
- TubercuList:   Rv1407
- GeneTree:   EBGT00050000017628
- HOGENOM:   HBG726909
- OMA:   SEYGLET
- ProtClustDB:   CLSK871930
- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- Gene3D:   G3DSA:1.10.940.10

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB; SSF48013 NusB_RsmB_TIM44

EC number: NA

Molecular weight: Translated: 48494; Mature: 48363

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: ACT_SITE 394-394 BINDING 301-301 BINDING 325-325 BINDING 341-341

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPRSRGPRRRPLDPARRAAFETLRAVSARDAYANLVLPALLAQRGIGGRDAAFATELTY
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GTCRARGLLDAVIGAAAERSPQAIDPVLLDLLRLGTYQLLRTRVDAHAAVSTTVEQAGIE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FDSARAGFVNGVLRTIAGRDERSWVGELAPDAQNDPIGHAAFVHAHPRWIAQAFADALGA
CCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
AVGELEAVLASDDERPAVHLAARPGVLTAGELARAVRGTVGRYSPFAVYLPRGDPGRLAP
HHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
VRDGQALVQDEGSQLVARALTLAPVDGDTGRWLDLCAGPGGKTALLAGLGLQCAARVTAV
CCCCCHHHHCCCHHHHHHHHHEECCCCCCCCEEEEECCCCCCCHHHHHCCHHHHHEEEEE
EPSPHRADLVAQNTRGLPVELLRVDGRHTDLDPGFDRVLVDAPCTGLGALRRRPEARWRR
CCCCCCHHHEECCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHCCCHHHHCC
QPADVAALAKLQRELLSAAIALTRPGGVVLYATCSPHLAETVGAVADALRRHPVHALDTR
CCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCC
PLFEPVIAGLGEGPHVQLWPHRHGTDAMFAAALRRLT
HHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
TPRSRGPRRRPLDPARRAAFETLRAVSARDAYANLVLPALLAQRGIGGRDAAFATELTY
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
GTCRARGLLDAVIGAAAERSPQAIDPVLLDLLRLGTYQLLRTRVDAHAAVSTTVEQAGIE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FDSARAGFVNGVLRTIAGRDERSWVGELAPDAQNDPIGHAAFVHAHPRWIAQAFADALGA
CCCCCHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHH
AVGELEAVLASDDERPAVHLAARPGVLTAGELARAVRGTVGRYSPFAVYLPRGDPGRLAP
HHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCC
VRDGQALVQDEGSQLVARALTLAPVDGDTGRWLDLCAGPGGKTALLAGLGLQCAARVTAV
CCCCCHHHHCCCHHHHHHHHHEECCCCCCCCEEEEECCCCCCCHHHHHCCHHHHHEEEEE
EPSPHRADLVAQNTRGLPVELLRVDGRHTDLDPGFDRVLVDAPCTGLGALRRRPEARWRR
CCCCCCHHHEECCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCHHHHHCCCHHHHCC
QPADVAALAKLQRELLSAAIALTRPGGVVLYATCSPHLAETVGAVADALRRHPVHALDTR
CCHHHHHHHHHHHHHHHHHHHEECCCCEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCC
PLFEPVIAGLGEGPHVQLWPHRHGTDAMFAAALRRLT
HHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036