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The map label for this gene is priA

Identifier: 148661193

GI number: 148661193

Start: 1578923

End: 1580890

Strand: Direct

Name: priA

Synonym: MRA_1411

Alternate gene names: 148661193

Gene position: 1578923-1580890 (Clockwise)

Preceding gene: 148661192

Following gene: 148661195

Centisome position: 35.72

GC content: 72.71

Gene sequence:

>1968_bases
ATGTTGTCGGTGCCGCACCTGGACCGCGACTTCGACTACTTGGTGCCCGCCGAACACTCCGACGATGCCCAGCCGGGGGT
GCGGGTACGGGTGCGGTTTCACGGTCGGCTGGTCGACGGGTTTGTCCTAGAGCGCCGCAGCGACAGCGATCACCACGGCA
AGCTGGGCTGGCTGGATCGTGTGGTGTCGCCCGAACCGGTGCTCACCACGGAGATCCGCCGGTTGGTCGATGCGGTGGCG
GCGCGCTACGCCGGGACCCGCCAGGACGTATTGCGGCTCGCAGTGCCCGCCCGGCACGCACGGGTGGAGCGGGAAATCAC
CACGGCCCCGGGTCGGCCGGTGGTAGCGCCGGTCGACCCGTCGGGTTGGGCGGCCTACGGTCGCGGTCGGCAATTCCTGG
CCGCGCTGGCCGACTCGCGCGCTGCGCGGGCCGTTTGGCAGGCGCTACCGGGCGAGCTGTGGGCGGACCGATTCGCCGAG
GCTGCCGCGCAGACCGTACGTGCCGGGCGCACGGTACTGGCGATCGTGCCCGATCAGCGGGATCTGGACACCCTGTGGCA
GGCCGCGACGGCCCTCGTCGATGAGCACAGTGTGGTAGCACTGTCGGCCGGCCTGGGCCCGGAGGCACGCTATCGGCGCT
GGCTGGCCGCGTTGCGGGGCAGCGCGCGGCTGGTGATTGGCACCCGCAGCGCGGTGTTCGCGCCGTTGAGCGAGCTGGGC
CTGGTCATGGTCTGGGCCGACGCCGACGACTCCCTGGCTGAGCCGCGGGCACCCTATCCGCACGCCCGTGAGGTGGCGAT
GCTGCGGGCGCATCAGGCGCGGTGCGCAGCGCTGATCGGCGGCTACGCCCGCACGGCCGAGGCCCACGCGCTGGTGCGTA
GCGGCTGGGCGCACGACGTGGTTGCACCCCGGCCGGAGGTGCGTGCACGCTCTCCTCGCGTGGTTGCCCTCGACGACAGC
GGATACGACGACGCGCGAGACCCGGCCGCCCGCACCGCACGGCTACCGTCCATCGCGCTGCGCGCCGCGCGCTCAGCGCT
GCAGTCCGGGGCGCCGGTGCTGGTGCAGGTGCCGCGGCGCGGGTACATCCCCTCGCTGGCCTGCGGGCGCTGCCGGGCGA
TCGCTCGTTGCCGGTCGTGCACGGGTCCGCTATCGCTGCAAGGCGCCGGCTCGCCCGGTGCGGTATGTCGCTGGTGTGGA
CGGGTGGACCCGACACTGCGATGCGTGCGCTGTGGGTCGGACGTGGTGCGTGCCGTGGTGGTGGGGGCCCGGCGCACTGC
CGAAGAGCTCGGCCGGGCATTCCCGGGTACGGCGGTGATTACGTCGGCCGGCGACACCCTGGTGCCCCAGCTCGACGCCG
GCCCAGCCCTGGTGGTCGCCACTCCAGGAGCCGAACCCCGGGCGCCCGGCGGGTATGGGGCGGCGCTGCTGCTGGATAGC
TGGGCGCTGCTGGGCCGTCAAGACTTGCGCGCGGCCGAGGACGCGCTGTGGCGCTGGATGACGGCGGCCGCCCTGGTTCG
GCCGCGCGGGGCGGGCGGTGTGGTGACCGTGGTCGCCGAATCGTCCATTCCGACAGTGCAATCGCTGATCCGGTGGGATC
CGGTCGGTCACGCGGAGGCCGAACTGGCAGCCCGAACCGAAGTCGGCCTGCCGCCAAGTGTGCACATCGCTGCTCTTGAC
GGCCCTGCCGGCACCGTGACGGCATTGCTGGAGGCGGCTCGGCTGCCCGACCCGGATCGCCTCCAAGCCGATCTGCTGGG
CCCGGTGGACCTGCCACCCGGCGTCCGTCGCCCGGCGGGCATCCCCGCCGATGCGCCGGTCATCAGGATGTTGCTGCGGG
TGTGCCGCGAGCAGGGCCTGGAGTTGGCGGCGAGTCTGCGGCGCGGCATCGGTGTGCTCAGTGCGCGGCAAACCCGGCAA
ACCCGTAGCCTGGTTCGGGTACAGATTGACCCGCTGCATATCGGGTAA

Upstream 100 bases:

>100_bases
CAGCACCTACGGATAGCTAGCGGACCGGTTGTCTAGCAGCGGATCTCGCGGTCAAGCCCGCACGCCCGTCGAAGTAGAGC
CGATCGCGCGGGTGCTGCCG

Downstream 100 bases:

>100_bases
ACGGAGTAACCGCTAGCTCAACACTTCCGGGCGGTGAAGATAAGGTATTCCCACTGCATCACGCCGTCGCAGAGGTATTC
GCGACAAAGTTCGGTGATTT

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MLSVPHLDRDFDYLVPAEHSDDAQPGVRVRVRFHGRLVDGFVLERRSDSDHHGKLGWLDRVVSPEPVLTTEIRRLVDAVA
ARYAGTRQDVLRLAVPARHARVEREITTAPGRPVVAPVDPSGWAAYGRGRQFLAALADSRAARAVWQALPGELWADRFAE
AAAQTVRAGRTVLAIVPDQRDLDTLWQAATALVDEHSVVALSAGLGPEARYRRWLAALRGSARLVIGTRSAVFAPLSELG
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAHALVRSGWAHDVVAPRPEVRARSPRVVALDDS
GYDDARDPAARTARLPSIALRAARSALQSGAPVLVQVPRRGYIPSLACGRCRAIARCRSCTGPLSLQGAGSPGAVCRWCG
RVDPTLRCVRCGSDVVRAVVVGARRTAEELGRAFPGTAVITSAGDTLVPQLDAGPALVVATPGAEPRAPGGYGAALLLDS
WALLGRQDLRAAEDALWRWMTAAALVRPRGAGGVVTVVAESSIPTVQSLIRWDPVGHAEAELAARTEVGLPPSVHIAALD
GPAGTVTALLEAARLPDPDRLQADLLGPVDLPPGVRRPAGIPADAPVIRMLLRVCREQGLELAASLRRGIGVLSARQTRQ
TRSLVRVQIDPLHIG

Sequences:

>Translated_655_residues
MLSVPHLDRDFDYLVPAEHSDDAQPGVRVRVRFHGRLVDGFVLERRSDSDHHGKLGWLDRVVSPEPVLTTEIRRLVDAVA
ARYAGTRQDVLRLAVPARHARVEREITTAPGRPVVAPVDPSGWAAYGRGRQFLAALADSRAARAVWQALPGELWADRFAE
AAAQTVRAGRTVLAIVPDQRDLDTLWQAATALVDEHSVVALSAGLGPEARYRRWLAALRGSARLVIGTRSAVFAPLSELG
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAHALVRSGWAHDVVAPRPEVRARSPRVVALDDS
GYDDARDPAARTARLPSIALRAARSALQSGAPVLVQVPRRGYIPSLACGRCRAIARCRSCTGPLSLQGAGSPGAVCRWCG
RVDPTLRCVRCGSDVVRAVVVGARRTAEELGRAFPGTAVITSAGDTLVPQLDAGPALVVATPGAEPRAPGGYGAALLLDS
WALLGRQDLRAAEDALWRWMTAAALVRPRGAGGVVTVVAESSIPTVQSLIRWDPVGHAEAELAARTEVGLPPSVHIAALD
GPAGTVTALLEAARLPDPDRLQADLLGPVDLPPGVRRPAGIPADAPVIRMLLRVCREQGLELAASLRRGIGVLSARQTRQ
TRSLVRVQIDPLHIG
>Mature_655_residues
MLSVPHLDRDFDYLVPAEHSDDAQPGVRVRVRFHGRLVDGFVLERRSDSDHHGKLGWLDRVVSPEPVLTTEIRRLVDAVA
ARYAGTRQDVLRLAVPARHARVEREITTAPGRPVVAPVDPSGWAAYGRGRQFLAALADSRAARAVWQALPGELWADRFAE
AAAQTVRAGRTVLAIVPDQRDLDTLWQAATALVDEHSVVALSAGLGPEARYRRWLAALRGSARLVIGTRSAVFAPLSELG
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAHALVRSGWAHDVVAPRPEVRARSPRVVALDDS
GYDDARDPAARTARLPSIALRAARSALQSGAPVLVQVPRRGYIPSLACGRCRAIARCRSCTGPLSLQGAGSPGAVCRWCG
RVDPTLRCVRCGSDVVRAVVVGARRTAEELGRAFPGTAVITSAGDTLVPQLDAGPALVVATPGAEPRAPGGYGAALLLDS
WALLGRQDLRAAEDALWRWMTAAALVRPRGAGGVVTVVAESSIPTVQSLIRWDPVGHAEAELAARTEVGLPPSVHIAALD
GPAGTVTALLEAARLPDPDRLQADLLGPVDLPPGVRRPAGIPADAPVIRMLLRVCREQGLELAASLRRGIGVLSARQTRQ
TRSLVRVQIDPLHIG

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the helicase family. PriA subfamily

Homologues:

Organism=Escherichia coli, GI1790370, Length=370, Percent_Identity=28.6486486486486, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PRIA_MYCBO (P0A5A6)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855089.1
- ProteinModelPortal:   P0A5A6
- SMR:   P0A5A6
- EnsemblBacteria:   EBMYCT00000017032
- GeneID:   1090725
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1437
- GeneTree:   EBGT00050000017134
- HOGENOM:   HBG350750
- OMA:   QALVRWD
- ProtClustDB:   PRK14873
- BioCyc:   MBOV233413:MB1437-MONOMER

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 69840; Mature: 69840

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVPHLDRDFDYLVPAEHSDDAQPGVRVRVRFHGRLVDGFVLERRSDSDHHGKLGWLDR
CCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCEEECCHHEECCCCCCCCCCHHHHHH
VVSPEPVLTTEIRRLVDAVAARYAGTRQDVLRLAVPARHARVEREITTAPGRPVVAPVDP
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCC
SGWAAYGRGRQFLAALADSRAARAVWQALPGELWADRFAEAAAQTVRAGRTVLAIVPDQR
CCCCHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
DLDTLWQAATALVDEHSVVALSAGLGPEARYRRWLAALRGSARLVIGTRSAVFAPLSELG
CHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHCHHHCC
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAHALVRSGWAHDV
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VAPRPEVRARSPRVVALDDSGYDDARDPAARTARLPSIALRAARSALQSGAPVLVQVPRR
CCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCC
GYIPSLACGRCRAIARCRSCTGPLSLQGAGSPGAVCRWCGRVDPTLRCVRCGSDVVRAVV
CCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHH
VGARRTAEELGRAFPGTAVITSAGDTLVPQLDAGPALVVATPGAEPRAPGGYGAALLLDS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHH
WALLGRQDLRAAEDALWRWMTAAALVRPRGAGGVVTVVAESSIPTVQSLIRWDPVGHAEA
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCHH
ELAARTEVGLPPSVHIAALDGPAGTVTALLEAARLPDPDRLQADLLGPVDLPPGVRRPAG
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCC
IPADAPVIRMLLRVCREQGLELAASLRRGIGVLSARQTRQTRSLVRVQIDPLHIG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECEECC
>Mature Secondary Structure
MLSVPHLDRDFDYLVPAEHSDDAQPGVRVRVRFHGRLVDGFVLERRSDSDHHGKLGWLDR
CCCCCCCCCCCCEECCCCCCCCCCCCCEEEEEECCEEECCHHEECCCCCCCCCCHHHHHH
VVSPEPVLTTEIRRLVDAVAARYAGTRQDVLRLAVPARHARVEREITTAPGRPVVAPVDP
CCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEECCC
SGWAAYGRGRQFLAALADSRAARAVWQALPGELWADRFAEAAAQTVRAGRTVLAIVPDQR
CCCCHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCC
DLDTLWQAATALVDEHSVVALSAGLGPEARYRRWLAALRGSARLVIGTRSAVFAPLSELG
CHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCHHHHHCHHHCC
LVMVWADADDSLAEPRAPYPHAREVAMLRAHQARCAALIGGYARTAEAHALVRSGWAHDV
EEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VAPRPEVRARSPRVVALDDSGYDDARDPAARTARLPSIALRAARSALQSGAPVLVQVPRR
CCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCC
GYIPSLACGRCRAIARCRSCTGPLSLQGAGSPGAVCRWCGRVDPTLRCVRCGSDVVRAVV
CCCCCCHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHCCCCHHHHHHHHCHHHHHHHH
VGARRTAEELGRAFPGTAVITSAGDTLVPQLDAGPALVVATPGAEPRAPGGYGAALLLDS
HHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHH
WALLGRQDLRAAEDALWRWMTAAALVRPRGAGGVVTVVAESSIPTVQSLIRWDPVGHAEA
HHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCHH
ELAARTEVGLPPSVHIAALDGPAGTVTALLEAARLPDPDRLQADLLGPVDLPPGVRRPAG
HHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCC
IPADAPVIRMLLRVCREQGLELAASLRRGIGVLSARQTRQTRSLVRVQIDPLHIG
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972