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The map label for this gene is carA [H]

Identifier: 148661174

GI number: 148661174

Start: 1557281

End: 1558411

Strand: Direct

Name: carA [H]

Synonym: MRA_1392

Alternate gene names: 148661174

Gene position: 1557281-1558411 (Clockwise)

Preceding gene: 148661173

Following gene: 148661175

Centisome position: 35.23

GC content: 67.73

Gene sequence:

>1131_bases
TTGAGCAAAGCCGTATTGGTCCTCGAAGACGGCCGGGTGTTCACCGGCAGGCCGTTCGGCGCGACCGGACAAGCGCTCGG
GGAGGCCGTGTTTTCCACCGGCATGTCCGGTTATCAGGAGACGCTGACCGATCCCAGCTATCACCGTCAGATCGTGGTGG
CCACCGCGCCGCAGATCGGCAACACCGGCTGGAACGGCGAGGACTCCGAAAGCCGAGGGGAGCGGATCTGGGTCGCCGGT
TACGCGGTGCGCGACCCGTCGCCGCGCGCGTCCAACTGGCGCGCCACCGGCACGTTGGAAGACGAACTCATCCGCCAGCG
CATCGTCGGGATCGCCGGCATCGACACCCGGGCCGTGGTGCGCCATCTGCGCAGCCGCGGGTCGATGAAGGCGGGGGTGT
TCTCCGACGGGGCGCTGGCCGAGCCTGCCGACTTGATCGCGCGGGTGCGAGCACAACAGTCGATGCTGGGCGCCGATCTG
GCCGGCGAGGTCAGCACCGCGGAGCCGTATGTCGTCGAACCCGACGGGCCACCGGGTGTTTCGAGGTTCACCGTGGCCGC
CCTAGATCTTGGTATCAAGACCAACACTCCGCGTAACTTCGCCCGGCGCGGGATTCGCTGCCATGTGCTGCCGGCATCGA
CCACCTTCGAGCAGATCGCCGAACTCAACCCGCATGGCGTGTTCTTGTCCAACGGCCCCGGCGACCCGGCCACCGCCGAT
CACGTCGTCGCGCTTACCCGCGAGGTGCTGGGCGCCGGAATCCCGTTGTTCGGCATCTGTTTCGGCAACCAGATCCTGGG
CCGCGCGCTGGGCCTGTCGACCTACAAGATGGTGTTTGGGCACCGCGGCATCAACATCCCGGTCGTCGACCACGCCACCG
GTCGGGTGGCGGTGACCGCGCAAAACCATGGCTTCGCCCTTCAGGGGGAGGCGGGCCAATCCTTCGCCACCCCGTTCGGT
CCCGCGGTGGTCAGCCACACCTGCGCCAACGACGGTGTGGTCGAAGGCGTCAAGCTCGTTGACGGGCGGGCGTTTTCGGT
GCAATACCACCCGGAAGCCGCCGCCGGCCCGCACGATGCCGAGTACCTGTTCGACCAGTTCGTGGAGCTGATGGCAGGGG
AGGGCCGCTAG

Upstream 100 bases:

>100_bases
TACTCGCGATCCGTTGGCGACTGCCGTCCGGCACCGAGATCGATGTCGGGTTTCGGGCAGACAACCGCGACGAATACCAG
GAGTGGCTGGAGGAACCCGT

Downstream 100 bases:

>100_bases
TGCCCCGTCGCACCGATCTGCACCACGTGCTGGTCATCGGCTCCGGGCCGATCGTCATCGGCCAGGCGTGCGAGTTCGAC
TACTCCGGGACTCAGGCGTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MSKAVLVLEDGRVFTGRPFGATGQALGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNGEDSESRGERIWVAG
YAVRDPSPRASNWRATGTLEDELIRQRIVGIAGIDTRAVVRHLRSRGSMKAGVFSDGALAEPADLIARVRAQQSMLGADL
AGEVSTAEPYVVEPDGPPGVSRFTVAALDLGIKTNTPRNFARRGIRCHVLPASTTFEQIAELNPHGVFLSNGPGDPATAD
HVVALTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVVDHATGRVAVTAQNHGFALQGEAGQSFATPFG
PAVVSHTCANDGVVEGVKLVDGRAFSVQYHPEAAAGPHDAEYLFDQFVELMAGEGR

Sequences:

>Translated_376_residues
MSKAVLVLEDGRVFTGRPFGATGQALGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNGEDSESRGERIWVAG
YAVRDPSPRASNWRATGTLEDELIRQRIVGIAGIDTRAVVRHLRSRGSMKAGVFSDGALAEPADLIARVRAQQSMLGADL
AGEVSTAEPYVVEPDGPPGVSRFTVAALDLGIKTNTPRNFARRGIRCHVLPASTTFEQIAELNPHGVFLSNGPGDPATAD
HVVALTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVVDHATGRVAVTAQNHGFALQGEAGQSFATPFG
PAVVSHTCANDGVVEGVKLVDGRAFSVQYHPEAAAGPHDAEYLFDQFVELMAGEGR
>Mature_375_residues
SKAVLVLEDGRVFTGRPFGATGQALGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIGNTGWNGEDSESRGERIWVAGY
AVRDPSPRASNWRATGTLEDELIRQRIVGIAGIDTRAVVRHLRSRGSMKAGVFSDGALAEPADLIARVRAQQSMLGADLA
GEVSTAEPYVVEPDGPPGVSRFTVAALDLGIKTNTPRNFARRGIRCHVLPASTTFEQIAELNPHGVFLSNGPGDPATADH
VVALTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVVDHATGRVAVTAQNHGFALQGEAGQSFATPFGP
AVVSHTCANDGVVEGVKLVDGRAFSVQYHPEAAAGPHDAEYLFDQFVELMAGEGR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=378, Percent_Identity=40.4761904761905, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI21361331, Length=380, Percent_Identity=34.7368421052632, Blast_Score=199, Evalue=2e-51,
Organism=Homo sapiens, GI169790915, Length=380, Percent_Identity=34.7368421052632, Blast_Score=199, Evalue=3e-51,
Organism=Escherichia coli, GI1786215, Length=386, Percent_Identity=45.5958549222798, Blast_Score=296, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI193204318, Length=378, Percent_Identity=38.0952380952381, Blast_Score=231, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6322331, Length=396, Percent_Identity=35.6060606060606, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6324878, Length=383, Percent_Identity=35.7702349869452, Blast_Score=213, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=38.560411311054, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=38.560411311054, Blast_Score=225, Evalue=4e-59,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39767; Mature: 39636

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAVLVLEDGRVFTGRPFGATGQALGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIG
CCCEEEEEECCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCC
NTGWNGEDSESRGERIWVAGYAVRDPSPRASNWRATGTLEDELIRQRIVGIAGIDTRAVV
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHH
RHLRSRGSMKAGVFSDGALAEPADLIARVRAQQSMLGADLAGEVSTAEPYVVEPDGPPGV
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCC
SRFTVAALDLGIKTNTPRNFARRGIRCHVLPASTTFEQIAELNPHGVFLSNGPGDPATAD
CCEEEEEEEECCCCCCCHHHHHCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCHH
HVVALTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVVDHATGRVAVTA
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCCEEEEEE
QNHGFALQGEAGQSFATPFGPAVVSHTCANDGVVEGVKLVDGRAFSVQYHPEAAAGPHDA
CCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCHHCCEEEECCEEEEEEECCCCCCCCCHH
EYLFDQFVELMAGEGR
HHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKAVLVLEDGRVFTGRPFGATGQALGEAVFSTGMSGYQETLTDPSYHRQIVVATAPQIG
CCEEEEEECCEEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCEEEEEECCCCC
NTGWNGEDSESRGERIWVAGYAVRDPSPRASNWRATGTLEDELIRQRIVGIAGIDTRAVV
CCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCEEECCCHHHHHHHHHHHHHCCCCHHHHH
RHLRSRGSMKAGVFSDGALAEPADLIARVRAQQSMLGADLAGEVSTAEPYVVEPDGPPGV
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCCC
SRFTVAALDLGIKTNTPRNFARRGIRCHVLPASTTFEQIAELNPHGVFLSNGPGDPATAD
CCEEEEEEEECCCCCCCHHHHHCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCCCCCHH
HVVALTREVLGAGIPLFGICFGNQILGRALGLSTYKMVFGHRGINIPVVDHATGRVAVTA
HHHHHHHHHHHCCCCEEEEHHHHHHHHHHHCHHHHHHHHCCCCCCEEEEECCCCEEEEEE
QNHGFALQGEAGQSFATPFGPAVVSHTCANDGVVEGVKLVDGRAFSVQYHPEAAAGPHDA
CCCCEEEECCCCCCCCCCCCHHHHHHHCCCCCHHCCEEEECCEEEEEEECCCCCCCCCHH
EYLFDQFVELMAGEGR
HHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]