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The map label for this gene is prfA [H]

Identifier: 148661087

GI number: 148661087

Start: 1456805

End: 1457878

Strand: Direct

Name: prfA [H]

Synonym: MRA_1307

Alternate gene names: 148661087

Gene position: 1456805-1457878 (Clockwise)

Preceding gene: 148661086

Following gene: 148661088

Centisome position: 32.96

GC content: 64.15

Gene sequence:

>1074_bases
ATGACGCAGCCAGTGCAGACGATTGACGTGTTGCTCGCCGAACACGCCGAGCTCGAGCTTGCGCTGGCAGATCCCGCGCT
GCACAGCAATCCGGCCGAGGCGCGCAGAGTCGGGCGCCGGTTTGCCCGATTGGCCCCGATCGTCGCAACCCACCGCAAGC
TGACGTCCGCGCGCGACGACCTCGAGACCGCGCGCGAGCTGGTGGCTTCCGACGAGTCGTTCGCCGCCGAGGTTGCCGCA
TTGGAGGCTCGGGTGGGCGAACTGGATGCCCAACTCACTGACATGTTGGCACCGCGTGACCCGCACGATGCCGATGACAT
TGTGCTGGAAGTCAAATCCGGCGAGGGGGGCGAAGAATCCGCGTTGTTCGCCGCCGATTTGGCCAGGATGTATATCCGCT
ACGCCGAGCGGCACGGCTGGGCGGTGACGGTGTTGGACGAGACCACCTCGGATCTGGGTGGGTACAAGGACGCGACGTTG
GCGATTGCCAGCAAAGCCGACACCCCCGACGGGGTGTGGTCGCGCATGAAGTTCGAGGGCGGGGTGCACCGCGTACAACG
GGTCCCAGTGACGGAATCCCAAGGCCGCGTGCATACTTCGGCGGCGGGTGTGCTGGTCTATCCGGAGCCCGAGGAAGTCG
GCCAAGTGCAGATCGACGAGTCGGATCTGCGTATCGACGTTTTCCGGTCGTCCGGCAAGGGCGGGCAGGGAGTGAATACC
ACCGACTCCGCGGTGCGTATCACCCATCTGCCCACTGGAATCGTCGTCACCTGTCAGAACGAACGGTCGCAGCTGCAGAA
CAAGACGCGTGCGTTGCAGGTGCTGGCCGCTCGGTTGCAGGCAATGGCCGAGGAGCAGGCGCTGGCCGACGCGTCGGCCG
ACCGGGCTAGCCAAATCCGCACTGTGGACCGTAGTGAACGCATTCGCACCTACAACTTCCCGGAGAACCGGATCACCGAC
CACCGGATCGGTTACAAGTCACACAATCTCGATCAGGTGCTGGATGGCGATCTTGACGCGTTGTTCGACGCTCTGTCCGC
CGCGGACAAGCAATCCCGGTTGCGACAATCATGA

Upstream 100 bases:

>100_bases
CCGGCAAATAGCTGGCTTACCGACGCCCGAACTGTGCACCAGCGGTACAGGACGGGCGTCGGTTCGCGTTAGGGTCCGCG
CTCGCGGGAAGAAGGTTGAC

Downstream 100 bases:

>100_bases
CCTCCGCGCCGGCGACGATGCGGTGGGGGAACCTCCCGCTTGCGGGGGAGAGCGGCACAATGACCCTGCGTCAGGCGATC
GACTTGGCTGCTGCGCTATT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MTQPVQTIDVLLAEHAELELALADPALHSNPAEARRVGRRFARLAPIVATHRKLTSARDDLETARELVASDESFAAEVAA
LEARVGELDAQLTDMLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWAVTVLDETTSDLGGYKDATL
AIASKADTPDGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVGQVQIDESDLRIDVFRSSGKGGQGVNT
TDSAVRITHLPTGIVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALADASADRASQIRTVDRSERIRTYNFPENRITD
HRIGYKSHNLDQVLDGDLDALFDALSAADKQSRLRQS

Sequences:

>Translated_357_residues
MTQPVQTIDVLLAEHAELELALADPALHSNPAEARRVGRRFARLAPIVATHRKLTSARDDLETARELVASDESFAAEVAA
LEARVGELDAQLTDMLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWAVTVLDETTSDLGGYKDATL
AIASKADTPDGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVGQVQIDESDLRIDVFRSSGKGGQGVNT
TDSAVRITHLPTGIVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALADASADRASQIRTVDRSERIRTYNFPENRITD
HRIGYKSHNLDQVLDGDLDALFDALSAADKQSRLRQS
>Mature_356_residues
TQPVQTIDVLLAEHAELELALADPALHSNPAEARRVGRRFARLAPIVATHRKLTSARDDLETARELVASDESFAAEVAAL
EARVGELDAQLTDMLAPRDPHDADDIVLEVKSGEGGEESALFAADLARMYIRYAERHGWAVTVLDETTSDLGGYKDATLA
IASKADTPDGVWSRMKFEGGVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVGQVQIDESDLRIDVFRSSGKGGQGVNTT
DSAVRITHLPTGIVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALADASADRASQIRTVDRSERIRTYNFPENRITDH
RIGYKSHNLDQVLDGDLDALFDALSAADKQSRLRQS

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=279, Percent_Identity=46.5949820788531, Blast_Score=246, Evalue=3e-65,
Organism=Homo sapiens, GI34577120, Length=362, Percent_Identity=37.5690607734807, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI166795305, Length=176, Percent_Identity=46.5909090909091, Blast_Score=159, Evalue=3e-39,
Organism=Escherichia coli, GI1787462, Length=348, Percent_Identity=44.8275862068966, Blast_Score=273, Evalue=2e-74,
Organism=Escherichia coli, GI2367172, Length=337, Percent_Identity=35.9050445103858, Blast_Score=201, Evalue=8e-53,
Organism=Caenorhabditis elegans, GI17542784, Length=240, Percent_Identity=39.5833333333333, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6321295, Length=331, Percent_Identity=38.6706948640483, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI19921226, Length=346, Percent_Identity=38.150289017341, Blast_Score=213, Evalue=2e-55,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 39037; Mature: 38906

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPVQTIDVLLAEHAELELALADPALHSNPAEARRVGRRFARLAPIVATHRKLTSARDD
CCCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETARELVASDESFAAEVAALEARVGELDAQLTDMLAPRDPHDADDIVLEVKSGEGGEES
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
ALFAADLARMYIRYAERHGWAVTVLDETTSDLGGYKDATLAIASKADTPDGVWSRMKFEG
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHCC
GVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVGQVQIDESDLRIDVFRSSGKGGQGVNT
CHHHHHCCCCCCCCCCEEECCCEEEEECCHHHCCEEEECCCCCEEEEEECCCCCCCCCCC
TDSAVRITHLPTGIVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALADASADRASQIR
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TVDRSERIRTYNFPENRITDHRIGYKSHNLDQVLDGDLDALFDALSAADKQSRLRQS
HCCHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQPVQTIDVLLAEHAELELALADPALHSNPAEARRVGRRFARLAPIVATHRKLTSARDD
CCCHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LETARELVASDESFAAEVAALEARVGELDAQLTDMLAPRDPHDADDIVLEVKSGEGGEES
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHH
ALFAADLARMYIRYAERHGWAVTVLDETTSDLGGYKDATLAIASKADTPDGVWSRMKFEG
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHCCCCCCEEEEEECCCCCHHHHHHHHHCC
GVHRVQRVPVTESQGRVHTSAAGVLVYPEPEEVGQVQIDESDLRIDVFRSSGKGGQGVNT
CHHHHHCCCCCCCCCCEEECCCEEEEECCHHHCCEEEECCCCCEEEEEECCCCCCCCCCC
TDSAVRITHLPTGIVVTCQNERSQLQNKTRALQVLAARLQAMAEEQALADASADRASQIR
CCCEEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
TVDRSERIRTYNFPENRITDHRIGYKSHNLDQVLDGDLDALFDALSAADKQSRLRQS
HCCHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA