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The map label for this gene is purB [H]

Identifier: 148660554

GI number: 148660554

Start: 871314

End: 872732

Strand: Direct

Name: purB [H]

Synonym: MRA_0786

Alternate gene names: 148660554

Gene position: 871314-872732 (Clockwise)

Preceding gene: 148660552

Following gene: 148660555

Centisome position: 19.71

GC content: 65.61

Gene sequence:

>1419_bases
GTGAGCATTCCCAACGTGCTGGCCACCCGATACGCCAGCGCCGAGATGGTCGCGATCTGGTCGCCGGAGGCCAAGGTGGT
CTCGGAGCGGCGGTTATGGCTGGCCGTATTGCGGGCACAGGCAGAGCTGGGGGTAGCGGTTGCCGATTCGGTGCTCGCCG
ACTACGAACGTGTGGTCGACGATGTGGACTTGGCCTCGATCTCAGCCCGGGAGCGGGTGCTGCGCCACGATGTCAAGGCC
CGCATCGAGGAATTCAACGCATTGGCCGGTCATGAGCACGTGCACAAGGGGATGACCAGCCGCGACCTGACCGAGAACGT
GGAGCAACTGCAGATTCGGCGGTCGCTGGAAGTGATTTTCGCCCATGGGGTGGCGGCGGTGGCGCGGCTGGCCGAGCGGG
CGGTGAGCTACCGTGACCTGATCATGGCCGGGCGCAGCCACAACGTGGCCGCTCAGGCCACCACCTTGGGCAAGCGGTTC
GCCTCGGCGGCCCAAGAGATGATGATCGCGTTGAGGCGGTTGAGGGAGTTGATCGACCGCTACCCCCTGCGTGGCATCAA
GGGCCCGATGGGCACCGGTCAGGACATGCTCGATCTGCTGGGCGGTGACCGTGCGGCGCTGGCCGATCTCGAGCGGCGCG
TCGCCGACTTCTTGGGCTTTGCAACTGTTTTCAACAGCGTGGGGCAGGTGTATCCGCGTTCATTGGACCACGACGTGGTT
TCGGCTCTGGTGCAGCTCGGCGCGGGGCCGTCATCACTGGCACACACGATTCGATTGATGGCCGGCCACGAGCTCGCCAC
CGAGGGTTTCGCGCCGGGTCAGGTCGGTTCGTCGGCGATGCCGCACAAGATGAACACCCGCAGCTGCGAACGGGTCAACG
GGCTGCAGGTTGTGCTACGCGGCTATGCATCCATGGTGGCCGAGTTAGCCGGTGCACAGTGGAACGAGGGTGATGTGTTT
TGCTCCGTGGTGCGCCGGGTTGCGTTGCCGGACAGCTTCTTTGCCGTCGACGGGCAGATCGAGACGTTTTTGACGGTGCT
GGACGAGTTCGGCGCCTACCCGGCGGTGATCGGCCGCGAGTTGGATCGTTATCTGCCGTTCCTGGCCACCACTAAGGTGC
TAATGGCGGCCGTGCGCGCGGGGATGGGTCGCGAGTCCGCGCACCGGTTGATCTCCGAGCACGCGGTGGCGACGGCGCTG
GCCATGCGAGAACACGGCGCGGAGCCCGACCTGCTGGACCGGTTGGCCGCCGATCCGCGGCTGACGCTGGGACGAGACGC
TTTGGAGGCCGCGCTGGCCGACAAGAAGGCATTTGCCGGTGCCGCGGGTGACCAGGTCGATGATGTGGTCGCGATGGTGG
ACGCGCTGGTGAGCCGTTACCCGGACGCGGCTAAATACACGCCGGGTGCAATTCTTTAG

Upstream 100 bases:

>100_bases
CGATGAGTCGGGCACATCGGGTGCTCCCTGGCGCCGGGACTCGTGTGACAACTGCGACTACTAGGCCCGCGACCGTAAGC
TGTGTCTTTGTGAGGGCCAA

Downstream 100 bases:

>100_bases
TGTCATGACTACCGCCGCCGGGCTTTCGGGCATCGATCTGACCGATCTGGACAACTTCGCCGACGGCTTCCCCCATCACC
TCTTCGCCATCCACCGTCGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSIPNVLATRYASAEMVAIWSPEAKVVSERRLWLAVLRAQAELGVAVADSVLADYERVVDDVDLASISARERVLRHDVKA
RIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLEVIFAHGVAAVARLAERAVSYRDLIMAGRSHNVAAQATTLGKRF
ASAAQEMMIALRRLRELIDRYPLRGIKGPMGTGQDMLDLLGGDRAALADLERRVADFLGFATVFNSVGQVYPRSLDHDVV
SALVQLGAGPSSLAHTIRLMAGHELATEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYASMVAELAGAQWNEGDVF
CSVVRRVALPDSFFAVDGQIETFLTVLDEFGAYPAVIGRELDRYLPFLATTKVLMAAVRAGMGRESAHRLISEHAVATAL
AMREHGAEPDLLDRLAADPRLTLGRDALEAALADKKAFAGAAGDQVDDVVAMVDALVSRYPDAAKYTPGAIL

Sequences:

>Translated_472_residues
MSIPNVLATRYASAEMVAIWSPEAKVVSERRLWLAVLRAQAELGVAVADSVLADYERVVDDVDLASISARERVLRHDVKA
RIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLEVIFAHGVAAVARLAERAVSYRDLIMAGRSHNVAAQATTLGKRF
ASAAQEMMIALRRLRELIDRYPLRGIKGPMGTGQDMLDLLGGDRAALADLERRVADFLGFATVFNSVGQVYPRSLDHDVV
SALVQLGAGPSSLAHTIRLMAGHELATEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYASMVAELAGAQWNEGDVF
CSVVRRVALPDSFFAVDGQIETFLTVLDEFGAYPAVIGRELDRYLPFLATTKVLMAAVRAGMGRESAHRLISEHAVATAL
AMREHGAEPDLLDRLAADPRLTLGRDALEAALADKKAFAGAAGDQVDDVVAMVDALVSRYPDAAKYTPGAIL
>Mature_471_residues
SIPNVLATRYASAEMVAIWSPEAKVVSERRLWLAVLRAQAELGVAVADSVLADYERVVDDVDLASISARERVLRHDVKAR
IEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLEVIFAHGVAAVARLAERAVSYRDLIMAGRSHNVAAQATTLGKRFA
SAAQEMMIALRRLRELIDRYPLRGIKGPMGTGQDMLDLLGGDRAALADLERRVADFLGFATVFNSVGQVYPRSLDHDVVS
ALVQLGAGPSSLAHTIRLMAGHELATEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLRGYASMVAELAGAQWNEGDVFC
SVVRRVALPDSFFAVDGQIETFLTVLDEFGAYPAVIGRELDRYLPFLATTKVLMAAVRAGMGRESAHRLISEHAVATALA
MREHGAEPDLLDRLAADPRLTLGRDALEAALADKKAFAGAAGDQVDDVVAMVDALVSRYPDAAKYTPGAIL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=442, Percent_Identity=31.6742081447964, Blast_Score=226, Evalue=5e-59,
Organism=Homo sapiens, GI183227688, Length=389, Percent_Identity=32.3907455012853, Blast_Score=206, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17508577, Length=466, Percent_Identity=27.6824034334764, Blast_Score=169, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32564234, Length=343, Percent_Identity=30.6122448979592, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323391, Length=449, Percent_Identity=34.7438752783964, Blast_Score=242, Evalue=8e-65,
Organism=Drosophila melanogaster, GI24647570, Length=465, Percent_Identity=33.1182795698925, Blast_Score=238, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51041; Mature: 50910

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPNVLATRYASAEMVAIWSPEAKVVSERRLWLAVLRAQAELGVAVADSVLADYERVVD
CCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVDLASISARERVLRHDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLEVIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AHGVAAVARLAERAVSYRDLIMAGRSHNVAAQATTLGKRFASAAQEMMIALRRLRELIDR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPLRGIKGPMGTGQDMLDLLGGDRAALADLERRVADFLGFATVFNSVGQVYPRSLDHDVV
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SALVQLGAGPSSLAHTIRLMAGHELATEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLR
HHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GYASMVAELAGAQWNEGDVFCSVVRRVALPDSFFAVDGQIETFLTVLDEFGAYPAVIGRE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHH
LDRYLPFLATTKVLMAAVRAGMGRESAHRLISEHAVATALAMREHGAEPDLLDRLAADPR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
LTLGRDALEAALADKKAFAGAAGDQVDDVVAMVDALVSRYPDAAKYTPGAIL
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SIPNVLATRYASAEMVAIWSPEAKVVSERRLWLAVLRAQAELGVAVADSVLADYERVVD
CCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DVDLASISARERVLRHDVKARIEEFNALAGHEHVHKGMTSRDLTENVEQLQIRRSLEVIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
AHGVAAVARLAERAVSYRDLIMAGRSHNVAAQATTLGKRFASAAQEMMIALRRLRELIDR
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YPLRGIKGPMGTGQDMLDLLGGDRAALADLERRVADFLGFATVFNSVGQVYPRSLDHDVV
CCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
SALVQLGAGPSSLAHTIRLMAGHELATEGFAPGQVGSSAMPHKMNTRSCERVNGLQVVLR
HHHHHHCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
GYASMVAELAGAQWNEGDVFCSVVRRVALPDSFFAVDGQIETFLTVLDEFGAYPAVIGRE
HHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHH
LDRYLPFLATTKVLMAAVRAGMGRESAHRLISEHAVATALAMREHGAEPDLLDRLAADPR
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCC
LTLGRDALEAALADKKAFAGAAGDQVDDVVAMVDALVSRYPDAAKYTPGAIL
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]