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The map label for this gene is pqqE [H]

Identifier: 148660468

GI number: 148660468

Start: 793463

End: 794638

Strand: Direct

Name: pqqE [H]

Synonym: MRA_0701

Alternate gene names: 148660468

Gene position: 793463-794638 (Clockwise)

Preceding gene: 148660467

Following gene: 148660469

Centisome position: 17.95

GC content: 65.99

Gene sequence:

>1176_bases
ATGACGAGCCCCGTACCCCGACTCATCGAGCAGTTCGAGCGGGGGCTCGACGCGCCGATCTGCCTTACCTGGGAGCTGAC
CTACGCCTGCAACCTAGCTTGCGTGCACTGCCTGTCGTCCTCGGGCAAACGCGATCCCGGCGAGTTGTCCACCCGCCAAT
GCAAGGACATCATCGACGAACTGGAACGCATGCAGGTGTTCTACGTGAACATCGGCGGCGGCGAACCAACCGTGCGCCCG
GACTTTTGGGAGCTGGTAGATTACGCCACCGCACACCACGTCGGGGTGAAATTCTCCACCAACGGGGTCCGGATCACCCC
CGAGGTGGCCACGCGGCTGGCAGCCACCGACTACGTCGACGTTCAGATCTCACTCGACGGCGCCACGGCCGAGGTCAACG
ACGCCATCCGCGGCACCGGGTCGTTCGACATGGCGGTGCGCGCGCTGCAGAACCTGGCAGCGGCGGGATTTGCCGGCGTC
AAGATCTCGGTTGTGATCACCCGGCGCAACGTCGCCCAGCTCGACGAATTCGCCACGCTGGCAAGCCGTTACGGAGCGAC
GTTGCGGATAACCAGGTTGCGACCGTCCGGGCGCGGGACTGACGTATGGGCCGACCTGCACCCCACCGCCGACCAGCAGG
TGCAGCTTTACGACTGGCTGGTTTCCAAAGGAGAGCGGGTGCTCACCGGCGATTCCTTCTTCCACCTGGCGCCGCTCGGC
CAGTCGGGGGCTCTGGCCGGCTTGAACATGTGCGGAGCCGGGCGGGTAGTGTGCCTGATCGACCCGGTGGGTGACGTGTA
TGCGTGCCCATTCGCCATTCATGACCACTTCTTAGCCGGAAACGTGTTGTCCGACGGCGGATTTCAAAATGTCTGGAAGA
ACTCGTCGCTGTTTCGCGAGCTCCGGGAGCCCCAGTCCGCAGGCGCCTGTGGCAGCTGCGGACACTACGACAGCTGCCGG
GGCGGCTGCATGGCGGCGAAATTCTTCACCGGCCTGCCGCTGGACGGGCCGGATCCCGAATGCGTGCAAGGCCATAGCGA
GCCGGCGCTGGCGCGCGAGCGCCACCTACCGCGGCCCCGCGCCGACCACTCCCGCGGTCGGCGCGTCAGCAAACCGGTGC
CCCTGACGCTGTCGATGCGGCCACCCAAGCGCCCGTGCAATGAAAGTCCGGTGTAG

Upstream 100 bases:

>100_bases
CCTGCCGCGGCGCCGGCGTCGACGACTGTGACCAGGATCCGTACCTGCACGCCCTGAGTGTGCTCGCCGGTTCGAACATG
CTGGTTCCTCGGCAGACAAC

Downstream 100 bases:

>100_bases
CCGTGGCCGAAGCGTGGTTTGAAACGGTAGCCATCGCGCAGCAACGCGCGAAGCGGAGGCTGCCGAAATCGGTTTACTCG
TCCCTGATTGCGGCCAGTGA

Product: coenzyme PQQ synthesis protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MTSPVPRLIEQFERGLDAPICLTWELTYACNLACVHCLSSSGKRDPGELSTRQCKDIIDELERMQVFYVNIGGGEPTVRP
DFWELVDYATAHHVGVKFSTNGVRITPEVATRLAATDYVDVQISLDGATAEVNDAIRGTGSFDMAVRALQNLAAAGFAGV
KISVVITRRNVAQLDEFATLASRYGATLRITRLRPSGRGTDVWADLHPTADQQVQLYDWLVSKGERVLTGDSFFHLAPLG
QSGALAGLNMCGAGRVVCLIDPVGDVYACPFAIHDHFLAGNVLSDGGFQNVWKNSSLFRELREPQSAGACGSCGHYDSCR
GGCMAAKFFTGLPLDGPDPECVQGHSEPALARERHLPRPRADHSRGRRVSKPVPLTLSMRPPKRPCNESPV

Sequences:

>Translated_391_residues
MTSPVPRLIEQFERGLDAPICLTWELTYACNLACVHCLSSSGKRDPGELSTRQCKDIIDELERMQVFYVNIGGGEPTVRP
DFWELVDYATAHHVGVKFSTNGVRITPEVATRLAATDYVDVQISLDGATAEVNDAIRGTGSFDMAVRALQNLAAAGFAGV
KISVVITRRNVAQLDEFATLASRYGATLRITRLRPSGRGTDVWADLHPTADQQVQLYDWLVSKGERVLTGDSFFHLAPLG
QSGALAGLNMCGAGRVVCLIDPVGDVYACPFAIHDHFLAGNVLSDGGFQNVWKNSSLFRELREPQSAGACGSCGHYDSCR
GGCMAAKFFTGLPLDGPDPECVQGHSEPALARERHLPRPRADHSRGRRVSKPVPLTLSMRPPKRPCNESPV
>Mature_390_residues
TSPVPRLIEQFERGLDAPICLTWELTYACNLACVHCLSSSGKRDPGELSTRQCKDIIDELERMQVFYVNIGGGEPTVRPD
FWELVDYATAHHVGVKFSTNGVRITPEVATRLAATDYVDVQISLDGATAEVNDAIRGTGSFDMAVRALQNLAAAGFAGVK
ISVVITRRNVAQLDEFATLASRYGATLRITRLRPSGRGTDVWADLHPTADQQVQLYDWLVSKGERVLTGDSFFHLAPLGQ
SGALAGLNMCGAGRVVCLIDPVGDVYACPFAIHDHFLAGNVLSDGGFQNVWKNSSLFRELREPQSAGACGSCGHYDSCRG
GCMAAKFFTGLPLDGPDPECVQGHSEPALARERHLPRPRADHSRGRRVSKPVPLTLSMRPPKRPCNESPV

Specific function: Required for production of the bacteriocin subtilosin. May activate a metalloenzyme that catalyzes a modification of prosubtilosin [H]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786999, Length=210, Percent_Identity=27.6190476190476, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR008792
- InterPro:   IPR007197 [H]

Pfam domain/function: PF05402 PqqD; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 42449; Mature: 42317

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPVPRLIEQFERGLDAPICLTWELTYACNLACVHCLSSSGKRDPGELSTRQCKDIIDE
CCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LERMQVFYVNIGGGEPTVRPDFWELVDYATAHHVGVKFSTNGVRITPEVATRLAATDYVD
HHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEEECCCEEECHHHHHHHHHCCEEE
VQISLDGATAEVNDAIRGTGSFDMAVRALQNLAAAGFAGVKISVVITRRNVAQLDEFATL
EEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHH
ASRYGATLRITRLRPSGRGTDVWADLHPTADQQVQLYDWLVSKGERVLTGDSFFHLAPLG
HHHCCCEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEECCCEEEEECCC
QSGALAGLNMCGAGRVVCLIDPVGDVYACPFAIHDHFLAGNVLSDGGFQNVWKNSSLFRE
CCCCCCCCEECCCCCEEEEECCCCCCEECCHHHHHHHHCCCEECCCCCHHHHCCHHHHHH
LREPQSAGACGSCGHYDSCRGGCMAAKFFTGLPLDGPDPECVQGHSEPALARERHLPRPR
HHCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHCCCCCC
ADHSRGRRVSKPVPLTLSMRPPKRPCNESPV
CCHHCCCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
TSPVPRLIEQFERGLDAPICLTWELTYACNLACVHCLSSSGKRDPGELSTRQCKDIIDE
CCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHH
LERMQVFYVNIGGGEPTVRPDFWELVDYATAHHVGVKFSTNGVRITPEVATRLAATDYVD
HHCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEEECCCEEECHHHHHHHHHCCEEE
VQISLDGATAEVNDAIRGTGSFDMAVRALQNLAAAGFAGVKISVVITRRNVAQLDEFATL
EEEEECCCCCHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCHHHHHHHHHH
ASRYGATLRITRLRPSGRGTDVWADLHPTADQQVQLYDWLVSKGERVLTGDSFFHLAPLG
HHHCCCEEEEEEECCCCCCCCEEEECCCCCCCHHHHHHHHHHCCCEEEECCCEEEEECCC
QSGALAGLNMCGAGRVVCLIDPVGDVYACPFAIHDHFLAGNVLSDGGFQNVWKNSSLFRE
CCCCCCCCEECCCCCEEEEECCCCCCEECCHHHHHHHHCCCEECCCCCHHHHCCHHHHHH
LREPQSAGACGSCGHYDSCRGGCMAAKFFTGLPLDGPDPECVQGHSEPALARERHLPRPR
HHCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCCCCCCHHHHCCCCCCCHHHHHCCCCCC
ADHSRGRRVSKPVPLTLSMRPPKRPCNESPV
CCHHCCCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 10572140; 10809709; 10809710 [H]