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The map label for this gene is echA5 [C]

Identifier: 148660450

GI number: 148660450

Start: 776089

End: 776880

Strand: Direct

Name: echA5 [C]

Synonym: MRA_0685

Alternate gene names: 148660450

Gene position: 776089-776880 (Clockwise)

Preceding gene: 148660449

Following gene: 148660453

Centisome position: 17.56

GC content: 69.7

Gene sequence:

>792_bases
ATGAGTGATCTGGTGCGTGTGGAGCGCAAAGGTCGGGTGACCACGGTGATTCTGAACCGGCCGGCCTCCCGCAACGCGGT
CAACGGCCCGACCGCCGCGGCGTTGTGCGCGGCGTTCGAGCAATTCGACCGGGACGACGCCGCGTCGGTGGCCGTACTCT
GGGGTGCGGGTGGAACCTTTTGTGCGGGAGCCGATTTGAAGGCCTTTGGCACACCGGAGGCCAACTCTGTGCACCGGACG
GGTCCCGGCCCGATGGGGCCGTCACGAATGATGCTGTCCAAACCTGTGATCGCCGCCGTCAGCGGCTACGCCGTCGCCGG
GGGGCTGGAATTGGCACTGTGGTGCGACCTGCGGGTGGCCGAGGAAGACGCCGTGTTCGGTGTGTTTTGCCGTCGCTGGG
GGGTACCGCTCATCGACGGCGGCACCGTGCGACTGCCACGGCTGATCGGGCACAGCCGCGCGATGGACATGATCCTCACT
GGCCGTGGGGTGCCGGCCGACGAAGCGCTGGCCATGGGGTTGGCCAATCGGGTGGTGCCCAAGGGTCAAGCCCGACAGGC
GGCTGAGGAGTTGGCGGCGCAATTGGCCGCGCTGCCGCAGCAGTGTCTGCGATCGGATCGGCTGTCGGCGCTGCACCAGT
GGGGCCTGCCCGAGTCCGCGGCGCTCGACCTCGAGTTCGCCAGCATCGCGCGGGTGGCCGGCGAGGCGCTAGAGGGGGCG
AGACGGTTCGCCGCGGGTGCCGGTCGGCATGGGGCCCCGGCACCTCGGGCCGAACAGGGCGACACGCTTTAG

Upstream 100 bases:

>100_bases
TGCCCGCCGACTGGCCGGGCGCCGGGTTACGGGCGGCGTACCACGACTTCGCCACTGCAATGGCGAAACGACGCGATGCA
ACTCAACTCCTGGAGGTGAC

Downstream 100 bases:

>100_bases
GCGGGTACGGCTCAGACCAAGGCGAAGGTCCGTGCCGATGCCGGCGAGGGCCACGGCTGCGGAATGGGTCGTTGCCGGAC
AACCTGGGGCCACCAGAACC

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSDLVRVERKGRVTTVILNRPASRNAVNGPTAAALCAAFEQFDRDDAASVAVLWGAGGTFCAGADLKAFGTPEANSVHRT
GPGPMGPSRMMLSKPVIAAVSGYAVAGGLELALWCDLRVAEEDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILT
GRGVPADEALAMGLANRVVPKGQARQAAEELAAQLAALPQQCLRSDRLSALHQWGLPESAALDLEFASIARVAGEALEGA
RRFAAGAGRHGAPAPRAEQGDTL

Sequences:

>Translated_263_residues
MSDLVRVERKGRVTTVILNRPASRNAVNGPTAAALCAAFEQFDRDDAASVAVLWGAGGTFCAGADLKAFGTPEANSVHRT
GPGPMGPSRMMLSKPVIAAVSGYAVAGGLELALWCDLRVAEEDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILT
GRGVPADEALAMGLANRVVPKGQARQAAEELAAQLAALPQQCLRSDRLSALHQWGLPESAALDLEFASIARVAGEALEGA
RRFAAGAGRHGAPAPRAEQGDTL
>Mature_262_residues
SDLVRVERKGRVTTVILNRPASRNAVNGPTAAALCAAFEQFDRDDAASVAVLWGAGGTFCAGADLKAFGTPEANSVHRTG
PGPMGPSRMMLSKPVIAAVSGYAVAGGLELALWCDLRVAEEDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILTG
RGVPADEALAMGLANRVVPKGQARQAAEELAAQLAALPQQCLRSDRLSALHQWGLPESAALDLEFASIARVAGEALEGAR
RFAAGAGRHGAPAPRAEQGDTL

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=199, Percent_Identity=35.1758793969849, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI70995211, Length=188, Percent_Identity=34.0425531914894, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI68989263, Length=153, Percent_Identity=37.2549019607843, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI20127408, Length=191, Percent_Identity=31.9371727748691, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI31542718, Length=196, Percent_Identity=27.0408163265306, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI4757968, Length=210, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI49355787, Length=210, Percent_Identity=25.7142857142857, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI4502327, Length=205, Percent_Identity=34.1463414634146, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=197, Percent_Identity=39.0862944162437, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI221142681, Length=198, Percent_Identity=38.8888888888889, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788597, Length=184, Percent_Identity=32.6086956521739, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788682, Length=194, Percent_Identity=35.0515463917526, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787660, Length=211, Percent_Identity=34.5971563981043, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790281, Length=208, Percent_Identity=31.25, Blast_Score=75, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=47.3469387755102, Blast_Score=220, Evalue=6e-58,
Organism=Caenorhabditis elegans, GI25145438, Length=246, Percent_Identity=30.8943089430894, Blast_Score=100, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17554946, Length=207, Percent_Identity=34.2995169082126, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17534483, Length=189, Percent_Identity=35.978835978836, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=180, Percent_Identity=31.6666666666667, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=208, Percent_Identity=29.3269230769231, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535521, Length=228, Percent_Identity=28.0701754385965, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=188, Percent_Identity=34.5744680851064, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=188, Percent_Identity=34.5744680851064, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=188, Percent_Identity=34.5744680851064, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=194, Percent_Identity=30.9278350515464, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=198, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17536985, Length=252, Percent_Identity=25.3968253968254, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21357171, Length=209, Percent_Identity=49.2822966507177, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24650670, Length=248, Percent_Identity=41.9354838709677, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI20129971, Length=210, Percent_Identity=35.7142857142857, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653477, Length=210, Percent_Identity=35.7142857142857, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24653139, Length=191, Percent_Identity=36.6492146596859, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI19921000, Length=271, Percent_Identity=31.7343173431734, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583077, Length=271, Percent_Identity=31.7343173431734, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24583079, Length=271, Percent_Identity=31.7343173431734, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27442; Mature: 27311

Theoretical pI: Translated: 7.89; Mature: 7.89

Prosite motif: PS00086 CYTOCHROME_P450 ; PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLVRVERKGRVTTVILNRPASRNAVNGPTAAALCAAFEQFDRDDAASVAVLWGAGGTF
CCCHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCE
CAGADLKAFGTPEANSVHRTGPGPMGPSRMMLSKPVIAAVSGYAVAGGLELALWCDLRVA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCEEEECCCEEEEEEEEEEC
EEDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILTGRGVPADEALAMGLANRVVP
CCCHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCC
KGQARQAAEELAAQLAALPQQCLRSDRLSALHQWGLPESAALDLEFASIARVAGEALEGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHH
RRFAAGAGRHGAPAPRAEQGDTL
HHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDLVRVERKGRVTTVILNRPASRNAVNGPTAAALCAAFEQFDRDDAASVAVLWGAGGTF
CCHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCE
CAGADLKAFGTPEANSVHRTGPGPMGPSRMMLSKPVIAAVSGYAVAGGLELALWCDLRVA
ECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCHHHHHCCEEEECCCEEEEEEEEEEC
EEDAVFGVFCRRWGVPLIDGGTVRLPRLIGHSRAMDMILTGRGVPADEALAMGLANRVVP
CCCHHHHHHHHHCCCCEECCCCEEHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCC
KGQARQAAEELAAQLAALPQQCLRSDRLSALHQWGLPESAALDLEFASIARVAGEALEGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHHHHHHHHHH
RRFAAGAGRHGAPAPRAEQGDTL
HHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA