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The map label for this gene is echA4 [C]

Identifier: 148660448

GI number: 148660448

Start: 774429

End: 775367

Strand: Direct

Name: echA4 [C]

Synonym: MRA_0683

Alternate gene names: 148660448

Gene position: 774429-775367 (Clockwise)

Preceding gene: 148660447

Following gene: 148660449

Centisome position: 17.52

GC content: 67.31

Gene sequence:

>939_bases
ATGACACACGCGATCAGGCCGGTCGATTTCGACAACCTGAAGACGATGACCTATGAGGTCACCGGTCGGATTGCGCGGAT
CACCTTCAACCGGCCGGAGAAGGGCAACGCGATCATCGCAGACACCCCGCTGGAGTTGTCTGCTCTGGTGGAGCGTGCCG
ATCTGGATCCAGGCGTGCATGTCATTCTGGTGTCCGGTCGCGGCGAGGGATTCTGTGCCGGCTTCGACCTGTCCGCCTAC
GCCGAGGGGTCGTCGTCGACCGGGGGCGGCGGCGCATACCAAGGCACGGTGCTAGATGGCAAGACCCAGGCCGTCAACCA
CCTACCGAACCAGCCGTGGGACCCGATGATCGACTACCAGATGATGAGCCGGTTCGTGCGCGGATTCGCCAGTCTGATGC
ATGCCGACAAGCCGACGGTGGTCAAGATCCACGGCTACTGCGTGGCCGGCGGCACCGACATCGCGCTGCACGCCGATCAG
GTGATCGCCGCCGCCGACGCCAAGATCGGCTACCCGCCCACCCGGGTGTGGGGGGTGCCGGCGGCGGGCCTGTGGGCGCA
CCGGCTCGGCGACCAGCGGGCCAAACGGCTGCTGTTCACCGGCGATTGCATCACCGGCGCGCAGGCCGCCGAGTGGGGCC
TGGCGGTCGAGGCGCCGGAGCCGGCTGACCTCGACGAGCGGACCGAGCGACTGGTGGCCCGGATCGCCGCACTGCCGGTC
AATCAATTGATCATGGTCAAGCTCGCGCTCAATTCCGCTCTGCTGCAACAGGGTGTGGCCACCAGCAGGATGGTCAGCAC
CGTGTTCGACGGCGCCGCTCGGCACACACCCGAGGGGCACGCGTTTGTCGCCGACGCGGTCGAGCACGGCTTCCGGGATG
CGGTGCGGCGCCGTGACGAGCCGTTTGGCGACTACGGCCGTCAAGCATCGCGGGTGTAA

Upstream 100 bases:

>100_bases
CTCGGCGGCCAGTGGGGCGGAGCGTACGGCACCATGCCGGCCGGTCTGGATCTCGCGCCCATCCTCGAGCGTGCGCTGGT
AAAAGGCTGAGCGGCCGCTG

Downstream 100 bases:

>100_bases
CCATGCCGGCCATGACCGCCCGTTCGGTGGTACTCAGCGTGCTGCTCGGTGCTCATCCCGCGTGGGCCACCGCAAGCGAA
TTGATCCAGCTGACAGCGGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MTHAIRPVDFDNLKTMTYEVTGRIARITFNRPEKGNAIIADTPLELSALVERADLDPGVHVILVSGRGEGFCAGFDLSAY
AEGSSSTGGGGAYQGTVLDGKTQAVNHLPNQPWDPMIDYQMMSRFVRGFASLMHADKPTVVKIHGYCVAGGTDIALHADQ
VIAAADAKIGYPPTRVWGVPAAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPEPADLDERTERLVARIAALPV
NQLIMVKLALNSALLQQGVATSRMVSTVFDGAARHTPEGHAFVADAVEHGFRDAVRRRDEPFGDYGRQASRV

Sequences:

>Translated_312_residues
MTHAIRPVDFDNLKTMTYEVTGRIARITFNRPEKGNAIIADTPLELSALVERADLDPGVHVILVSGRGEGFCAGFDLSAY
AEGSSSTGGGGAYQGTVLDGKTQAVNHLPNQPWDPMIDYQMMSRFVRGFASLMHADKPTVVKIHGYCVAGGTDIALHADQ
VIAAADAKIGYPPTRVWGVPAAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPEPADLDERTERLVARIAALPV
NQLIMVKLALNSALLQQGVATSRMVSTVFDGAARHTPEGHAFVADAVEHGFRDAVRRRDEPFGDYGRQASRV
>Mature_311_residues
THAIRPVDFDNLKTMTYEVTGRIARITFNRPEKGNAIIADTPLELSALVERADLDPGVHVILVSGRGEGFCAGFDLSAYA
EGSSSTGGGGAYQGTVLDGKTQAVNHLPNQPWDPMIDYQMMSRFVRGFASLMHADKPTVVKIHGYCVAGGTDIALHADQV
IAAADAKIGYPPTRVWGVPAAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPEPADLDERTERLVARIAALPVN
QLIMVKLALNSALLQQGVATSRMVSTVFDGAARHTPEGHAFVADAVEHGFRDAVRRRDEPFGDYGRQASRV

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Escherichia coli, GI1787660, Length=238, Percent_Identity=27.3109243697479, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 33541; Mature: 33410

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHAIRPVDFDNLKTMTYEVTGRIARITFNRPEKGNAIIADTPLELSALVERADLDPGVH
CCCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCEE
VILVSGRGEGFCAGFDLSAYAEGSSSTGGGGAYQGTVLDGKTQAVNHLPNQPWDPMIDYQ
EEEEECCCCCEEECCCCCEECCCCCCCCCCCEEECEEECCCHHHHHCCCCCCCCCCCCHH
MMSRFVRGFASLMHADKPTVVKIHGYCVAGGTDIALHADQVIAAADAKIGYPPTRVWGVP
HHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEECCCEEEECCCCCCCCCCCEECCC
AAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPEPADLDERTERLVARIAALPV
CHHHHHHHHCHHHHHEEEEECCCCCCCCHHHCCEEECCCCCCCHHHHHHHHHHHHHHCCC
NQLIMVKLALNSALLQQGVATSRMVSTVFDGAARHTPEGHAFVADAVEHGFRDAVRRRDE
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCEEHHHHHHHHHHHHHHHCCC
PFGDYGRQASRV
CCCHHCHHHCCC
>Mature Secondary Structure 
THAIRPVDFDNLKTMTYEVTGRIARITFNRPEKGNAIIADTPLELSALVERADLDPGVH
CCCCCCCCCCCCEEEEEEECCEEEEEEECCCCCCCEEEEECCHHHHHHHHHHCCCCCEE
VILVSGRGEGFCAGFDLSAYAEGSSSTGGGGAYQGTVLDGKTQAVNHLPNQPWDPMIDYQ
EEEEECCCCCEEECCCCCEECCCCCCCCCCCEEECEEECCCHHHHHCCCCCCCCCCCCHH
MMSRFVRGFASLMHADKPTVVKIHGYCVAGGTDIALHADQVIAAADAKIGYPPTRVWGVP
HHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEECCCEEEECCCCCCCCCCCEECCC
AAGLWAHRLGDQRAKRLLFTGDCITGAQAAEWGLAVEAPEPADLDERTERLVARIAALPV
CHHHHHHHHCHHHHHEEEEECCCCCCCCHHHCCEEECCCCCCCHHHHHHHHHHHHHHCCC
NQLIMVKLALNSALLQQGVATSRMVSTVFDGAARHTPEGHAFVADAVEHGFRDAVRRRDE
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHCCCCCCEEHHHHHHHHHHHHHHHCCC
PFGDYGRQASRV
CCCHHCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA