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The map label for this gene is fadE8 [H]

Identifier: 148660447

GI number: 148660447

Start: 772790

End: 774418

Strand: Direct

Name: fadE8 [H]

Synonym: MRA_0682

Alternate gene names: 148660447

Gene position: 772790-774418 (Clockwise)

Preceding gene: 148660446

Following gene: 148660448

Centisome position: 17.48

GC content: 66.91

Gene sequence:

>1629_bases
ATGTCAGATACGCATGTCGTCACCAACCAGGTTCCGCCCTTGGAGAACTACAATCCCGCGTCATCCCCGGTGCTCATCGA
GGCTCTGATCCAGGAGGGTGGCCAGTGGGGCCTGGATGAAGTAAACGAGGTCGGGGCAATTTCTGCCAGCTGCCAAGCCC
AACGCTGGGGAGAGCTTGCAGACCGCAACCGGCCCATCCTGCATACCCACGACGCTTACGGGTACCGGGTCGATGAGGTG
GAGTACGACCCGGCCTACCACGAGCTGATGCGTACCGCGATCACCCATGGCATGCACGCCGCACCGTGGGCTGACGACCG
CCCGGGTGCGCACGTGGTGCGAGCGGCCAAGACATCGGTGTGGACCGTCGAGCCGGGCCATATCTGCCCCATCTCGATGA
CCTACGCCGTCGTTCCGGCGCTGCGGTATAACTCCGAGCTGGCTGCGGTCTACGAGCCGCTGCTGACCAGTCGTGAGTAC
GACCCGGAGCTGAAGCCGGCGACCACGAAGGCCGGCATCACCGCCGGCATGTCGATGACCGAGAAGCAGGGTGGCTCCGA
CGTGCGCGCTGGCACCACCCAGGCGACCCCGAATGCGGACGGCAGCTACAGCTTGACCGGCCACAAGTGGTTCACTTCGG
CGCCGATGTGCGACATCTTCCTGGTGCTCGCGCAGGCACCGGACGGGCTGTCGTGCTTCCTGCTGCCGCGGGTGCTGCCC
GACGGCACCCGCAACCGAATGTTCTTGCAGCGGCTCAAGGACAAGCTCGGCAACCACGCAAACGCCTCGAGCGAGGTCGA
ATACGACGGTGCCGTCGCGTGGCTGGTGGGCGAGGAGGGCCGCGGCGTGCCGACCATCATCGAGATGGTCAACCTCACCC
GGCTGGACTGCGCTCTGGGCAGTGCCACCAGCATGCGCACCGGCCTAACCCGCGCCGTCCACCATGCCCAGCATCGGAAG
GCGTTCGGCGCCTACCTGATCGACCAGCCGTTGATGCGCAACGTGCTGGCCGACCTGGCGGTGGAGGCCGAGGCCGCCAC
CATCGTGGCAATGCGGATGGCCGGTGCCACCGACAACGCGGTGCGCGGGAACGAGACCGAAGCGCTGCTGCGTCGCATCG
GCCTGGCGGCCGCCAAGTACTGGGTGTGCAAGCGCTCCACCGCTCACGCCGCCGAAGCGCTGGAGTGCCTGGGCGGCAAC
GGTTATGTCGAGGATTCCGGGATGCCCCGGCTCTACCGGGAGGCGCCGTTGATGGGCATCTGGGAGGGCTCGGGCAATGT
CAGCGCGCTAGATACCTTGCGCGCCATGGCAACCCGGCCCGCATGCGTCGAGGTGCTGTTTGACGAGCTGGCCCGCAGCG
CAGGCCAGGACCCCAGGCTGGACGGCCACGTCGAAAGGCTGCGTCCGCAGCTGGGCGATCTTGACACGATCGGTTATCGA
GCCCGCAAGATTGCCGAAGACATCTGCCTGGCGTTGCAGGGATCGTTGTTGGTGCGCCACGGACATCCCGCCGTCGCCGA
GGCGTTTCTGGCCACTCGGCTCGGCGGCCAGTGGGGCGGAGCGTACGGCACCATGCCGGCCGGTCTGGATCTCGCGCCCA
TCCTCGAGCGTGCGCTGGTAAAAGGCTGA

Upstream 100 bases:

>100_bases
CCCAGACACTGTGGCGATTCTTTGCAGCGCACTTCAGATGAGGACAAAACCATCCGTTACATTCTCTTGTGCAGTTGTAG
AAAAAACGTAACATGGTGGC

Downstream 100 bases:

>100_bases
GCGGCCGCTGATGACACACGCGATCAGGCCGGTCGATTTCGACAACCTGAAGACGATGACCTATGAGGTCACCGGTCGGA
TTGCGCGGATCACCTTCAAC

Product: acyl-CoA dehydrogenase FadE8

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MSDTHVVTNQVPPLENYNPASSPVLIEALIQEGGQWGLDEVNEVGAISASCQAQRWGELADRNRPILHTHDAYGYRVDEV
EYDPAYHELMRTAITHGMHAAPWADDRPGAHVVRAAKTSVWTVEPGHICPISMTYAVVPALRYNSELAAVYEPLLTSREY
DPELKPATTKAGITAGMSMTEKQGGSDVRAGTTQATPNADGSYSLTGHKWFTSAPMCDIFLVLAQAPDGLSCFLLPRVLP
DGTRNRMFLQRLKDKLGNHANASSEVEYDGAVAWLVGEEGRGVPTIIEMVNLTRLDCALGSATSMRTGLTRAVHHAQHRK
AFGAYLIDQPLMRNVLADLAVEAEAATIVAMRMAGATDNAVRGNETEALLRRIGLAAAKYWVCKRSTAHAAEALECLGGN
GYVEDSGMPRLYREAPLMGIWEGSGNVSALDTLRAMATRPACVEVLFDELARSAGQDPRLDGHVERLRPQLGDLDTIGYR
ARKIAEDICLALQGSLLVRHGHPAVAEAFLATRLGGQWGGAYGTMPAGLDLAPILERALVKG

Sequences:

>Translated_542_residues
MSDTHVVTNQVPPLENYNPASSPVLIEALIQEGGQWGLDEVNEVGAISASCQAQRWGELADRNRPILHTHDAYGYRVDEV
EYDPAYHELMRTAITHGMHAAPWADDRPGAHVVRAAKTSVWTVEPGHICPISMTYAVVPALRYNSELAAVYEPLLTSREY
DPELKPATTKAGITAGMSMTEKQGGSDVRAGTTQATPNADGSYSLTGHKWFTSAPMCDIFLVLAQAPDGLSCFLLPRVLP
DGTRNRMFLQRLKDKLGNHANASSEVEYDGAVAWLVGEEGRGVPTIIEMVNLTRLDCALGSATSMRTGLTRAVHHAQHRK
AFGAYLIDQPLMRNVLADLAVEAEAATIVAMRMAGATDNAVRGNETEALLRRIGLAAAKYWVCKRSTAHAAEALECLGGN
GYVEDSGMPRLYREAPLMGIWEGSGNVSALDTLRAMATRPACVEVLFDELARSAGQDPRLDGHVERLRPQLGDLDTIGYR
ARKIAEDICLALQGSLLVRHGHPAVAEAFLATRLGGQWGGAYGTMPAGLDLAPILERALVKG
>Mature_541_residues
SDTHVVTNQVPPLENYNPASSPVLIEALIQEGGQWGLDEVNEVGAISASCQAQRWGELADRNRPILHTHDAYGYRVDEVE
YDPAYHELMRTAITHGMHAAPWADDRPGAHVVRAAKTSVWTVEPGHICPISMTYAVVPALRYNSELAAVYEPLLTSREYD
PELKPATTKAGITAGMSMTEKQGGSDVRAGTTQATPNADGSYSLTGHKWFTSAPMCDIFLVLAQAPDGLSCFLLPRVLPD
GTRNRMFLQRLKDKLGNHANASSEVEYDGAVAWLVGEEGRGVPTIIEMVNLTRLDCALGSATSMRTGLTRAVHHAQHRKA
FGAYLIDQPLMRNVLADLAVEAEAATIVAMRMAGATDNAVRGNETEALLRRIGLAAAKYWVCKRSTAHAAEALECLGGNG
YVEDSGMPRLYREAPLMGIWEGSGNVSALDTLRAMATRPACVEVLFDELARSAGQDPRLDGHVERLRPQLGDLDTIGYRA
RKIAEDICLALQGSLLVRHGHPAVAEAFLATRLGGQWGGAYGTMPAGLDLAPILERALVKG

Specific function: May help to prevent alkylation damage by protecting DNA and destroying alkylating agents [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI76496475, Length=306, Percent_Identity=27.1241830065359, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4557235, Length=306, Percent_Identity=27.1241830065359, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI4557233, Length=263, Percent_Identity=28.8973384030418, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI226958414, Length=297, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI226958412, Length=297, Percent_Identity=26.2626262626263, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI21361497, Length=385, Percent_Identity=25.1948051948052, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI4501859, Length=284, Percent_Identity=26.056338028169, Blast_Score=73, Evalue=8e-13,
Organism=Homo sapiens, GI4503943, Length=287, Percent_Identity=27.1777003484321, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI7656849, Length=273, Percent_Identity=28.9377289377289, Blast_Score=69, Evalue=9e-12,
Organism=Homo sapiens, GI7669494, Length=256, Percent_Identity=27.734375, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI87082384, Length=520, Percent_Identity=44.2307692307692, Blast_Score=404, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI86563383, Length=490, Percent_Identity=30.2040816326531, Blast_Score=187, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI86563381, Length=490, Percent_Identity=30.2040816326531, Blast_Score=187, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17538396, Length=290, Percent_Identity=27.9310344827586, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17508101, Length=299, Percent_Identity=25.752508361204, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71985184, Length=285, Percent_Identity=25.6140350877193, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17506239, Length=268, Percent_Identity=27.2388059701493, Blast_Score=86, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17569725, Length=276, Percent_Identity=24.6376811594203, Blast_Score=80, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71985192, Length=224, Percent_Identity=25.8928571428571, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17570075, Length=282, Percent_Identity=24.113475177305, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI281363737, Length=398, Percent_Identity=26.1306532663317, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21356377, Length=263, Percent_Identity=25.8555133079848, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24666513, Length=294, Percent_Identity=24.8299319727891, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646207, Length=281, Percent_Identity=24.9110320284698, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355753, Length=265, Percent_Identity=24.9056603773585, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24660351, Length=277, Percent_Identity=25.2707581227437, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M [H]

EC number: NA

Molecular weight: Translated: 58535; Mature: 58404

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00073 ACYL_COA_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTHVVTNQVPPLENYNPASSPVLIEALIQEGGQWGLDEVNEVGAISASCQAQRWGELA
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
DRNRPILHTHDAYGYRVDEVEYDPAYHELMRTAITHGMHAAPWADDRPGAHVVRAAKTSV
HCCCCEEEECCCCCEEECCEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEE
WTVEPGHICPISMTYAVVPALRYNSELAAVYEPLLTSREYDPELKPATTKAGITAGMSMT
EEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHH
EKQGGSDVRAGTTQATPNADGSYSLTGHKWFTSAPMCDIFLVLAQAPDGLSCFLLPRVLP
HHCCCCCCCCCCCCCCCCCCCCEEECCCCEECCCCHHHHHHHHHCCCCCCEEEEEHHHCC
DGTRNRMFLQRLKDKLGNHANASSEVEYDGAVAWLVGEEGRGVPTIIEMVNLTRLDCALG
CCCCHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHC
SATSMRTGLTRAVHHAQHRKAFGAYLIDQPLMRNVLADLAVEAEAATIVAMRMAGATDNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCC
VRGNETEALLRRIGLAAAKYWVCKRSTAHAAEALECLGGNGYVEDSGMPRLYREAPLMGI
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHCCCEEE
WEGSGNVSALDTLRAMATRPACVEVLFDELARSAGQDPRLDGHVERLRPQLGDLDTIGYR
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHCCCHHHHHH
ARKIAEDICLALQGSLLVRHGHPAVAEAFLATRLGGQWGGAYGTMPAGLDLAPILERALV
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KG
CC
>Mature Secondary Structure 
SDTHVVTNQVPPLENYNPASSPVLIEALIQEGGQWGLDEVNEVGAISASCQAQRWGELA
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHH
DRNRPILHTHDAYGYRVDEVEYDPAYHELMRTAITHGMHAAPWADDRPGAHVVRAAKTSV
HCCCCEEEECCCCCEEECCEECCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHCCCCEE
WTVEPGHICPISMTYAVVPALRYNSELAAVYEPLLTSREYDPELKPATTKAGITAGMSMT
EEECCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCHH
EKQGGSDVRAGTTQATPNADGSYSLTGHKWFTSAPMCDIFLVLAQAPDGLSCFLLPRVLP
HHCCCCCCCCCCCCCCCCCCCCEEECCCCEECCCCHHHHHHHHHCCCCCCEEEEEHHHCC
DGTRNRMFLQRLKDKLGNHANASSEVEYDGAVAWLVGEEGRGVPTIIEMVNLTRLDCALG
CCCCHHHHHHHHHHHHCCCCCCCCCEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHC
SATSMRTGLTRAVHHAQHRKAFGAYLIDQPLMRNVLADLAVEAEAATIVAMRMAGATDNA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHCCCCCCC
VRGNETEALLRRIGLAAAKYWVCKRSTAHAAEALECLGGNGYVEDSGMPRLYREAPLMGI
CCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCCCHHHHHCCCEEE
WEGSGNVSALDTLRAMATRPACVEVLFDELARSAGQDPRLDGHVERLRPQLGDLDTIGYR
EECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHCHHHCCCHHHHHH
ARKIAEDICLALQGSLLVRHGHPAVAEAFLATRLGGQWGGAYGTMPAGLDLAPILERALV
HHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
KG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961409; 7610040; 9278503 [H]