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The map label for this gene is 148660428

Identifier: 148660428

GI number: 148660428

Start: 751306

End: 752811

Strand: Direct

Name: 148660428

Synonym: MRA_0665

Alternate gene names: NA

Gene position: 751306-752811 (Clockwise)

Preceding gene: 148660426

Following gene: 148660429

Centisome position: 17.0

GC content: 66.8

Gene sequence:

>1506_bases
ATGACCACCGCACAAGCCGCCGAATCCCAAAACCCATATCTCGAGGGCTTCCTGGCGCCGGTGAGCACCGAGGTAACTGC
CACCGACCTGCCGGTCACCGGCCGCATTCCGGAACACCTCGACGGGCGTTATCTGCGTAACGGCCCCAACCCGGTCGCGG
AGGTCGACCCGGCCACCTACCACTGGTTCACCGGCGACGCCATGGTGCACGGAGTCGCGCTGCGCGACGGGAAGGCCCGC
TGGTATCGCAATCGCTGGGTCCGCACACCCGCGGTGTGCGCCGCCCTGGGCGAGCCCATTTCGGCCCGGCCTCACCCGCG
CACCGGGATTATCGAGGGCGGTCCCAACACCAACGTGCTGACCCACGCCGGACGCACCCTGGCCTTGGTTGAGGCCGGCG
TGGTCAACTACGAACTCACCGATGAGCTGGACACCGTGGGACCCTGTGACTTCGACGGCACCCTGCACGGCGGTTACACC
GCCCATCCGCAGCGTGATCCGCACACGGGTGAACTGCACGCGGTGTCCTACTCGTTCGCCCGCGGACACAGAGTGCAGTA
CTCGGTGATCGGCACCGACGGACACGCTCGTCGGACGGTTGATATCGAGGTGGCGGGATCGCCGATGATGCACAGCTTCT
CCCTGACCGACAACTACGTGGTGATCTACGACCTGCCGGTGACCTTCGACCCAATGCAGGTGGTGCCGGCGTCCGTGCCA
CGCTGGCTGCAACGGCCCGCCAGGTTGGTGATCCAGTCGGTCCTGGGCCGTGTCCGCATCCCCGACCCGATAGCGGCGTT
GGGCAACCGGATGCAGGGTCACTCCGATCGCCTCCCGTACGCCTGGAACCCCAGCTACCCGGCGCGCGTCGGTGTCATGC
CGCGCGAGGGTGGCAACGAGGACGTGCGGTGGTTCGACATCGAACCCTGCTACGTATACCACCCACTTAACGCCTACTCG
GAGTGCCGGAACGGCGCTGAGGTGCTGGTGTTGGACGTGGTGCGCTACTCACGGATGTTTGATCGCGACCGGCGGGGTCC
CGGCGGTGACAGCCGGCCCTCGCTGGATCGCTGGACCATCAACCTGGCGACCGGTGCGGTGACCGCCGAATGCCGCGACG
ATCGGGCGCAGGAGTTTCCCCGCATCAACGAGACTCTGGTGGGTGGGCCGCATCGCTTCGCCTACACCGTCGGCATCGAG
GGTGGGTTTCTCGTCGGCGCCGGCGCTGCGTTGTCGACTCCGCTGTATAAACAGGACTGCGTGACCGGGTCCAGCACGGT
CGCCTCGCTCGATCCCGACCTGCTGATCGGCGAGATGGTGTTCGTGCCGAACCCGTCGGCGCGTGCAGAAGATGACGGGA
TTCTCATGGGCTACGGCTGGCACCGCGGCCGCGACGAAGGCCAGCTGCTCTTGCTGGATGCCCAGACTCTCGAGTCGATC
GCCACCGTGCACCTGCCACAGCGTGTGCCGATGGGCTTCCACGGCAACTGGGCGCCGACCACCTGA

Upstream 100 bases:

>100_bases
CTCGTCGCGAACACCGGTCTGCGAGGTCATCCTTGCCATGTTCTCACCAAGGGCGTACCGTTCCAATATCAGTGAAATAA
CAATGTTATAGGAGATCGGC

Downstream 100 bases:

>100_bases
CGGCGCCTCGGGTGCGATACAGTGACTCATACCACACAACGGGCCGGTGGCAGCCACGAGCGTCGACAGAAGGGTTTCCC
ATGGGCGTCAGCATCGAGGT

Product: putative dioxygenase

Products: NA

Alternate protein names: ACO; 8'-apo-beta-carotenal 15,15'-oxygenase; Diox1 [H]

Number of amino acids: Translated: 501; Mature: 500

Protein sequence:

>501_residues
MTTAQAAESQNPYLEGFLAPVSTEVTATDLPVTGRIPEHLDGRYLRNGPNPVAEVDPATYHWFTGDAMVHGVALRDGKAR
WYRNRWVRTPAVCAALGEPISARPHPRTGIIEGGPNTNVLTHAGRTLALVEAGVVNYELTDELDTVGPCDFDGTLHGGYT
AHPQRDPHTGELHAVSYSFARGHRVQYSVIGTDGHARRTVDIEVAGSPMMHSFSLTDNYVVIYDLPVTFDPMQVVPASVP
RWLQRPARLVIQSVLGRVRIPDPIAALGNRMQGHSDRLPYAWNPSYPARVGVMPREGGNEDVRWFDIEPCYVYHPLNAYS
ECRNGAEVLVLDVVRYSRMFDRDRRGPGGDSRPSLDRWTINLATGAVTAECRDDRAQEFPRINETLVGGPHRFAYTVGIE
GGFLVGAGAALSTPLYKQDCVTGSSTVASLDPDLLIGEMVFVPNPSARAEDDGILMGYGWHRGRDEGQLLLLDAQTLESI
ATVHLPQRVPMGFHGNWAPTT

Sequences:

>Translated_501_residues
MTTAQAAESQNPYLEGFLAPVSTEVTATDLPVTGRIPEHLDGRYLRNGPNPVAEVDPATYHWFTGDAMVHGVALRDGKAR
WYRNRWVRTPAVCAALGEPISARPHPRTGIIEGGPNTNVLTHAGRTLALVEAGVVNYELTDELDTVGPCDFDGTLHGGYT
AHPQRDPHTGELHAVSYSFARGHRVQYSVIGTDGHARRTVDIEVAGSPMMHSFSLTDNYVVIYDLPVTFDPMQVVPASVP
RWLQRPARLVIQSVLGRVRIPDPIAALGNRMQGHSDRLPYAWNPSYPARVGVMPREGGNEDVRWFDIEPCYVYHPLNAYS
ECRNGAEVLVLDVVRYSRMFDRDRRGPGGDSRPSLDRWTINLATGAVTAECRDDRAQEFPRINETLVGGPHRFAYTVGIE
GGFLVGAGAALSTPLYKQDCVTGSSTVASLDPDLLIGEMVFVPNPSARAEDDGILMGYGWHRGRDEGQLLLLDAQTLESI
ATVHLPQRVPMGFHGNWAPTT
>Mature_500_residues
TTAQAAESQNPYLEGFLAPVSTEVTATDLPVTGRIPEHLDGRYLRNGPNPVAEVDPATYHWFTGDAMVHGVALRDGKARW
YRNRWVRTPAVCAALGEPISARPHPRTGIIEGGPNTNVLTHAGRTLALVEAGVVNYELTDELDTVGPCDFDGTLHGGYTA
HPQRDPHTGELHAVSYSFARGHRVQYSVIGTDGHARRTVDIEVAGSPMMHSFSLTDNYVVIYDLPVTFDPMQVVPASVPR
WLQRPARLVIQSVLGRVRIPDPIAALGNRMQGHSDRLPYAWNPSYPARVGVMPREGGNEDVRWFDIEPCYVYHPLNAYSE
CRNGAEVLVLDVVRYSRMFDRDRRGPGGDSRPSLDRWTINLATGAVTAECRDDRAQEFPRINETLVGGPHRFAYTVGIEG
GFLVGAGAALSTPLYKQDCVTGSSTVASLDPDLLIGEMVFVPNPSARAEDDGILMGYGWHRGRDEGQLLLLDAQTLESIA
TVHLPQRVPMGFHGNWAPTT

Specific function: Cleaves a number of carotenals and carotenols in the all-trans configuration at the 15-15' double bond producing retinal or retinol, respectively. Also shows activity toward lycopenals and the corresponding alcohols. Does not cleave beta- carotene or lyco

COG id: COG3670

COG function: function code Q; Lignostilbene-alpha,beta-dioxygenase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carotenoid oxygenase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004294 [H]

Pfam domain/function: PF03055 RPE65 [H]

EC number: =1.14.99.41 [H]

Molecular weight: Translated: 54944; Mature: 54813

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTAQAAESQNPYLEGFLAPVSTEVTATDLPVTGRIPEHLDGRYLRNGPNPVAEVDPATY
CCCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHCCCCEECCCCCCCCCCCCCEE
HWFTGDAMVHGVALRDGKARWYRNRWVRTPAVCAALGEPISARPHPRTGIIEGGPNTNVL
EEEECCCEEEEEEEECCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEECCCCCCEE
THAGRTLALVEAGVVNYELTDELDTVGPCDFDGTLHGGYTAHPQRDPHTGELHAVSYSFA
EECCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECC
RGHRVQYSVIGTDGHARRTVDIEVAGSPMMHSFSLTDNYVVIYDLPVTFDPMQVVPASVP
CCCEEEEEEEECCCCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCHHHCCCHHH
RWLQRPARLVIQSVLGRVRIPDPIAALGNRMQGHSDRLPYAWNPSYPARVGVMPREGGNE
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCC
DVRWFDIEPCYVYHPLNAYSECRNGAEVLVLDVVRYSRMFDRDRRGPGGDSRPSLDRWTI
CCEEEECCCEEEEECCCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
NLATGAVTAECRDDRAQEFPRINETLVGGPHRFAYTVGIEGGFLVGAGAALSTPLYKQDC
EEECCEEEEHHHHHHHHHCCCCCHHHCCCCCEEEEEEECCCCEEEECCHHHCCCCHHHHC
VTGSSTVASLDPDLLIGEMVFVPNPSARAEDDGILMGYGWHRGRDEGQLLLLDAQTLESI
CCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEHHHHHHH
ATVHLPQRVPMGFHGNWAPTT
HHEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTAQAAESQNPYLEGFLAPVSTEVTATDLPVTGRIPEHLDGRYLRNGPNPVAEVDPATY
CCCCCCCCCCCCCEEEECCCCCCEEEECCCCCCCCCHHCCCCEECCCCCCCCCCCCCEE
HWFTGDAMVHGVALRDGKARWYRNRWVRTPAVCAALGEPISARPHPRTGIIEGGPNTNVL
EEEECCCEEEEEEEECCCHHHHHHHCCCCHHHHHHHCCCCCCCCCCCCCEEECCCCCCEE
THAGRTLALVEAGVVNYELTDELDTVGPCDFDGTLHGGYTAHPQRDPHTGELHAVSYSFA
EECCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCEEEEEEECC
RGHRVQYSVIGTDGHARRTVDIEVAGSPMMHSFSLTDNYVVIYDLPVTFDPMQVVPASVP
CCCEEEEEEEECCCCCEEEEEEEECCCCCEEEEECCCCEEEEEECCCCCCCHHHCCCHHH
RWLQRPARLVIQSVLGRVRIPDPIAALGNRMQGHSDRLPYAWNPSYPARVGVMPREGGNE
HHHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCC
DVRWFDIEPCYVYHPLNAYSECRNGAEVLVLDVVRYSRMFDRDRRGPGGDSRPSLDRWTI
CCEEEECCCEEEEECCCHHHHHCCCCEEEEEHHHHHHHHHHHCCCCCCCCCCCCCCEEEE
NLATGAVTAECRDDRAQEFPRINETLVGGPHRFAYTVGIEGGFLVGAGAALSTPLYKQDC
EEECCEEEEHHHHHHHHHCCCCCHHHCCCCCEEEEEEECCCCEEEECCHHHCCCCHHHHC
VTGSSTVASLDPDLLIGEMVFVPNPSARAEDDGILMGYGWHRGRDEGQLLLLDAQTLESI
CCCCCEEECCCCCEEEEEEEEECCCCCCCCCCCEEEECCCCCCCCCCCEEEEEHHHHHHH
ATVHLPQRVPMGFHGNWAPTT
HHEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]