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The map label for this gene is recD

Identifier: 148660401

GI number: 148660401

Start: 721315

End: 723042

Strand: Reverse

Name: recD

Synonym: MRA_0638

Alternate gene names: 148660401

Gene position: 723042-721315 (Counterclockwise)

Preceding gene: 148660402

Following gene: 148660400

Centisome position: 16.36

GC content: 69.97

Gene sequence:

>1728_bases
GTGAAGCTCACCGACGTCGATTTTGCCGTCGAGGCCTCCGGGATGGTGCGGGCCTTCAACCAGGCCGGTGTGCTGGACGT
GTCGGATGTGCATGTGGCGCAACGTCTCTGTGCACTGGCCGGGGAGAGCGACGAGCGGGTGGCGTTGGCGGTAGCGGTGG
CGGTCCGCGCGCTACGGGCCGGTTCGGTGTGTGTGGATCTGTTGTCGATCGCGCGGGTTGCTGGACACGACGACCTGCCG
TGGCCGGATCCGGCGGACTGGTTGGCGGCGGTGCGGGCCAGTCCGTTGCTCGCCGATCCACCCGTGCTGCACCTGTATGA
CGATCGGCTGCTCTATCTGGACCGCTACTGGCGCGAAGAAGAGCAGGTGTGCGCGGACCTGCTCGCGTTGCTGACATCCA
GGCGACCGGCCGGGGTACCCGACTTGCGGCGACTGTTCCCGACCGGCTTTGACGAGCAGCGGCGCGCGGCGGAAATCGCG
CTCTCGCAAGGTGTTACGGTGTTGACTGGCGGGCCTGGCACCGGGAAGACGACGACGGTCGCGCGGCTGCTGGCGTTGGT
TGCCGAGCAGGCGGAGCTGGCCGGTGAGCCGCGGCCGCGGATCGCGCTGGCCGCACCCACCGGCAAGGCGGCGGCCCGGC
TGGCCGAGGCGGTACGGCGCGAGATGGCCAAGCTCGACGCCACCGACCGGGCACGGCTCGGCGACCTGCACGCGGTCACG
CTGCATCGTCTGCTGGGTGCCAAACCGGGAGCGCGGTTTCGCCAGGATCGCCAGAACCGGTTGCCTCACAACGTGATCGT
GGTCGACGAGACCTCGATGGTGTCGCTGACGCTGATGGCCCGGCTGGCCGAGGCGGTGCGCCCGGGCGCCCGGTTGATCC
TCGTCGGCGATGCCGACCAGTTGGCGTCGGTGGAAGCCGGAGCGGTGCTGGCGGATTTGGTCGATGGCTTCTCGGTACGC
GACGACGCGCTGGTGGCGCAGCTGCGAACGTCGCATCGGTTCGGCAAGGTGATCGGTACCTTGGCCGAAGCGATCCGGGC
CGGCGATGGCGATGCCGTACTGGGCCTGCTGCGCTCGGGTGAGGAGCGGATCGAGTTCGTCGACGACGAGGATCCGGCGC
CGCGGTTGCGCGCGGTGCTGGTGCCACACGCGTTGCGGCTGCGCGAGGCGGCGCTGTTGGGTGCCTCCGACGTCGCGTTG
GCCACCCTGGACGAGCACCGGCTGCTGTGTGCGCATCGGGACGGGCCCACCGGCGTGCTGCACTGGAACCGCCGGGTTCA
AGCCTGGCTCGCCGAGGAGACCGGCCAGCCGCCCTGGACGCCGTGGTACGCCGGGCGTCCGCTGCTGGTGACGGCCAACG
ACTACGGCCTTCGCGTCTACAACGGCGACACCGGTGTCGTGCTGGCCGGCCCCACCGGTCTGCGGGCCGTCATTTCTGGT
GCATCCGGGCCACTTGACGTTGCCACCGGCCGCCTCGGCGACGTCGAGACCATGCACGCGATGACCATCCACAAGAGTCA
GGGCAGCCAGGTCGACGAGGTGACGGTGCTGATGCCGCAAGAGGATTCGCGGTTGCTGACCCGGGAGTTGCTGTACACCG
CGGTGACCCGGGCCAAACGCAAGGTCCGGGTGGTCGGGTCCGAAGCTTCGGTGCGGGCCGCGATCGCACGCCGGGCGGTG
CGGGCCAGCGGGTTGCGGATGCGGTTACAGTCGACGGGGTGTGGCTGA

Upstream 100 bases:

>100_bases
CGGTGACGGCCGGGCATCCGGCCGGTGTGTTCACCTGGAACCCGCCCACGGCGTTGGTGGTGGCGCTCTCGGATCTGCTC
GACCGGGGAAGGCTGCAGTC

Downstream 100 bases:

>100_bases
CTCGTCGGGGATGTGCTCGGCTCGTCGGGTAACCGCGCCCGGACTTTGACATCGCATACACGAACAGCACGGCGACGGCG
CATCATGGATGTGTGCGGAT

Product: putative exonuclease V alpha chain

Products: NA

Alternate protein names: Exodeoxyribonuclease V 67 kDa polypeptide

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKLTDVDFAVEASGMVRAFNQAGVLDVSDVHVAQRLCALAGESDERVALAVAVAVRALRAGSVCVDLLSIARVAGHDDLP
WPDPADWLAAVRASPLLADPPVLHLYDDRLLYLDRYWREEEQVCADLLALLTSRRPAGVPDLRRLFPTGFDEQRRAAEIA
LSQGVTVLTGGPGTGKTTTVARLLALVAEQAELAGEPRPRIALAAPTGKAAARLAEAVRREMAKLDATDRARLGDLHAVT
LHRLLGAKPGARFRQDRQNRLPHNVIVVDETSMVSLTLMARLAEAVRPGARLILVGDADQLASVEAGAVLADLVDGFSVR
DDALVAQLRTSHRFGKVIGTLAEAIRAGDGDAVLGLLRSGEERIEFVDDEDPAPRLRAVLVPHALRLREAALLGASDVAL
ATLDEHRLLCAHRDGPTGVLHWNRRVQAWLAEETGQPPWTPWYAGRPLLVTANDYGLRVYNGDTGVVLAGPTGLRAVISG
ASGPLDVATGRLGDVETMHAMTIHKSQGSQVDEVTVLMPQEDSRLLTRELLYTAVTRAKRKVRVVGSEASVRAAIARRAV
RASGLRMRLQSTGCG

Sequences:

>Translated_575_residues
MKLTDVDFAVEASGMVRAFNQAGVLDVSDVHVAQRLCALAGESDERVALAVAVAVRALRAGSVCVDLLSIARVAGHDDLP
WPDPADWLAAVRASPLLADPPVLHLYDDRLLYLDRYWREEEQVCADLLALLTSRRPAGVPDLRRLFPTGFDEQRRAAEIA
LSQGVTVLTGGPGTGKTTTVARLLALVAEQAELAGEPRPRIALAAPTGKAAARLAEAVRREMAKLDATDRARLGDLHAVT
LHRLLGAKPGARFRQDRQNRLPHNVIVVDETSMVSLTLMARLAEAVRPGARLILVGDADQLASVEAGAVLADLVDGFSVR
DDALVAQLRTSHRFGKVIGTLAEAIRAGDGDAVLGLLRSGEERIEFVDDEDPAPRLRAVLVPHALRLREAALLGASDVAL
ATLDEHRLLCAHRDGPTGVLHWNRRVQAWLAEETGQPPWTPWYAGRPLLVTANDYGLRVYNGDTGVVLAGPTGLRAVISG
ASGPLDVATGRLGDVETMHAMTIHKSQGSQVDEVTVLMPQEDSRLLTRELLYTAVTRAKRKVRVVGSEASVRAAIARRAV
RASGLRMRLQSTGCG
>Mature_575_residues
MKLTDVDFAVEASGMVRAFNQAGVLDVSDVHVAQRLCALAGESDERVALAVAVAVRALRAGSVCVDLLSIARVAGHDDLP
WPDPADWLAAVRASPLLADPPVLHLYDDRLLYLDRYWREEEQVCADLLALLTSRRPAGVPDLRRLFPTGFDEQRRAAEIA
LSQGVTVLTGGPGTGKTTTVARLLALVAEQAELAGEPRPRIALAAPTGKAAARLAEAVRREMAKLDATDRARLGDLHAVT
LHRLLGAKPGARFRQDRQNRLPHNVIVVDETSMVSLTLMARLAEAVRPGARLILVGDADQLASVEAGAVLADLVDGFSVR
DDALVAQLRTSHRFGKVIGTLAEAIRAGDGDAVLGLLRSGEERIEFVDDEDPAPRLRAVLVPHALRLREAALLGASDVAL
ATLDEHRLLCAHRDGPTGVLHWNRRVQAWLAEETGQPPWTPWYAGRPLLVTANDYGLRVYNGDTGVVLAGPTGLRAVISG
ASGPLDVATGRLGDVETMHAMTIHKSQGSQVDEVTVLMPQEDSRLLTRELLYTAVTRAKRKVRVVGSEASVRAAIARRAV
RASGLRMRLQSTGCG

Specific function: Exhibits several catalytic activities, including ATP- dependent exonuclease, ATP-stimulated endonuclease, ATP-dependent unwinding and DNA-dependent ATPase activities. Strand cleavage occurs 5' to 3' during the unwinding of duplex DNA at CHI sequences, whi

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recD family

Homologues:

Organism=Escherichia coli, GI1789182, Length=581, Percent_Identity=36.6609294320138, Blast_Score=250, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): EX5A_MYCTU (P96919)

Other databases:

- EMBL:   BX842574
- EMBL:   AE000516
- PIR:   B70612
- RefSeq:   NP_215143.1
- RefSeq:   NP_335067.1
- ProteinModelPortal:   P96919
- SMR:   P96919
- EnsemblBacteria:   EBMYCT00000003008
- EnsemblBacteria:   EBMYCT00000070025
- GeneID:   887999
- GeneID:   925059
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0657
- KEGG:   mtu:Rv0629c
- TIGR:   MT0657
- TubercuList:   Rv0629c
- GeneTree:   EBGT00050000017311
- HOGENOM:   HBG482408
- OMA:   LPMMAKL
- ProtClustDB:   CLSK871834
- GO:   GO:0005829
- InterPro:   IPR007807
- InterPro:   IPR006344
- InterPro:   IPR000606
- TIGRFAMs:   TIGR01447

Pfam domain/function: PF05127 DUF699; PF01443 Viral_helicase1

EC number: =3.1.11.5

Molecular weight: Translated: 61748; Mature: 61748

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTDVDFAVEASGMVRAFNQAGVLDVSDVHVAQRLCALAGESDERVALAVAVAVRALRA
CCCCCCCEEEECCHHHHHHCCCCCEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
GSVCVDLLSIARVAGHDDLPWPDPADWLAAVRASPLLADPPVLHLYDDRLLYLDRYWREE
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCEEEEHHHHHCCH
EQVCADLLALLTSRRPAGVPDLRRLFPTGFDEQRRAAEIALSQGVTVLTGGPGTGKTTTV
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHH
ARLLALVAEQAELAGEPRPRIALAAPTGKAAARLAEAVRREMAKLDATDRARLGDLHAVT
HHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHH
LHRLLGAKPGARFRQDRQNRLPHNVIVVDETSMVSLTLMARLAEAVRPGARLILVGDADQ
HHHHHCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCHH
LASVEAGAVLADLVDGFSVRDDALVAQLRTSHRFGKVIGTLAEAIRAGDGDAVLGLLRSG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
EERIEFVDDEDPAPRLRAVLVPHALRLREAALLGASDVALATLDEHRLLCAHRDGPTGVL
CHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCEEE
HWNRRVQAWLAEETGQPPWTPWYAGRPLLVTANDYGLRVYNGDTGVVLAGPTGLRAVISG
EECHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEECCCCHHHHHCC
ASGPLDVATGRLGDVETMHAMTIHKSQGSQVDEVTVLMPQEDSRLLTRELLYTAVTRAKR
CCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
KVRVVGSEASVRAAIARRAVRASGLRMRLQSTGCG
EEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCC
>Mature Secondary Structure
MKLTDVDFAVEASGMVRAFNQAGVLDVSDVHVAQRLCALAGESDERVALAVAVAVRALRA
CCCCCCCEEEECCHHHHHHCCCCCEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
GSVCVDLLSIARVAGHDDLPWPDPADWLAAVRASPLLADPPVLHLYDDRLLYLDRYWREE
CHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCEEEEHHHHHCCH
EQVCADLLALLTSRRPAGVPDLRRLFPTGFDEQRRAAEIALSQGVTVLTGGPGTGKTTTV
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHHH
ARLLALVAEQAELAGEPRPRIALAAPTGKAAARLAEAVRREMAKLDATDRARLGDLHAVT
HHHHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHH
LHRLLGAKPGARFRQDRQNRLPHNVIVVDETSMVSLTLMARLAEAVRPGARLILVGDADQ
HHHHHCCCCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCEEEEEECCHH
LASVEAGAVLADLVDGFSVRDDALVAQLRTSHRFGKVIGTLAEAIRAGDGDAVLGLLRSG
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCC
EERIEFVDDEDPAPRLRAVLVPHALRLREAALLGASDVALATLDEHRLLCAHRDGPTGVL
CHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCCEEE
HWNRRVQAWLAEETGQPPWTPWYAGRPLLVTANDYGLRVYNGDTGVVLAGPTGLRAVISG
EECHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEECCCCEEEECCCCHHHHHCC
ASGPLDVATGRLGDVETMHAMTIHKSQGSQVDEVTVLMPQEDSRLLTRELLYTAVTRAKR
CCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHH
KVRVVGSEASVRAAIARRAVRASGLRMRLQSTGCG
EEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036