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The map label for this gene is yafV [C]

Identifier: 148660247

GI number: 148660247

Start: 570274

End: 571116

Strand: Reverse

Name: yafV [C]

Synonym: MRA_0487

Alternate gene names: 148660247

Gene position: 571116-570274 (Counterclockwise)

Preceding gene: 148660248

Following gene: 148660246

Centisome position: 12.92

GC content: 67.62

Gene sequence:

>843_bases
ATGCGAATCGCGTTGGCGCAAATCCGCAGCGGTACCGACCCCGCCGCCAATCTGCAACTGGTCGGCAAGTACGCCGGCGA
AGCCGCCACCGCGGGCGCACAGCTGGTGGTGTTTCCTGAGGCGACCATGTGCCGGCTCGGTGTCCCGCTGCGGCAGGTCG
CCGAGCCCGTCGACGGACCCTGGGCAAACGGAGTCCGACGGATCGCGACCGAGGCGGGCATCACCGTGATCGCCGGCATG
TTCACCCCGACCGGCGACGGGCGGGTAACAAACACGCTGATCGCAGCCGGCCCGGGCACGCCCAATCAGCCGGACGCGCA
CTACCACAAGATCCACCTCTATGACGCGTTCGGCTTCACCGAGTCACGTACCGTCGCACCCGGGCGCGAACCGGTGGTAG
TCGTGGTCGACGGCGTGCGGGTGGGTTTGACCGTTTGCTACGACATTCGCTTTCCCGCCCTTTATACCGAGCTGGCGCGG
CGCGGGGCCCAACTGATCGCGGTCTGTGCATCCTGGGGTTCCGGTCCGGGCAAACTCGAACAGTGGACGTTGCTGGCCCG
CGCCCGGGCGCTAGACTCCATGAGTTACGTCGCCGCGGCCGGCCAAGCAGACCCAGGTGATGCCCGCACCGGCGTGGGGG
CGAGCTCGGCTGCACCGACCGGGGTAGGCGGCAGCCTGGTGGCCTCGCCGCTAGGCGAGGTGGTGGTGTCAGCTGGCACC
CAGCCGCAACTGCTGGTCGCCGACATCGATGTCGACAATGTGGCCGCGGCTCGCGACCGCATTGCGGTGCTACGCAACCA
GACAGACTTCGTTCAGATCGATAAGGCACAATCGCGTGGGTGA

Upstream 100 bases:

>100_bases
CGCGCCGGTGCGGCGCGCTCGTCGCCGGGCGCAAGCTGGATTGCCCGGCTCCTGCGCGCGCCGGTGCGGCGCGCTCGTCG
CCGGGCCTAGGCTGGCGCGC

Downstream 100 bases:

>100_bases
CCAACCCGCAAGGTCCACCGAACGACCCGTCGCCCTGGGCGCGCCCTGGGGATCAAGGTCCGCTCGCCCGACCCCCGGCA
TCGTCCGAAGCATCCACCGG

Product: carbon-nitrogen hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRIALAQIRSGTDPAANLQLVGKYAGEAATAGAQLVVFPEATMCRLGVPLRQVAEPVDGPWANGVRRIATEAGITVIAGM
FTPTGDGRVTNTLIAAGPGTPNQPDAHYHKIHLYDAFGFTESRTVAPGREPVVVVVDGVRVGLTVCYDIRFPALYTELAR
RGAQLIAVCASWGSGPGKLEQWTLLARARALDSMSYVAAAGQADPGDARTGVGASSAAPTGVGGSLVASPLGEVVVSAGT
QPQLLVADIDVDNVAAARDRIAVLRNQTDFVQIDKAQSRG

Sequences:

>Translated_280_residues
MRIALAQIRSGTDPAANLQLVGKYAGEAATAGAQLVVFPEATMCRLGVPLRQVAEPVDGPWANGVRRIATEAGITVIAGM
FTPTGDGRVTNTLIAAGPGTPNQPDAHYHKIHLYDAFGFTESRTVAPGREPVVVVVDGVRVGLTVCYDIRFPALYTELAR
RGAQLIAVCASWGSGPGKLEQWTLLARARALDSMSYVAAAGQADPGDARTGVGASSAAPTGVGGSLVASPLGEVVVSAGT
QPQLLVADIDVDNVAAARDRIAVLRNQTDFVQIDKAQSRG
>Mature_280_residues
MRIALAQIRSGTDPAANLQLVGKYAGEAATAGAQLVVFPEATMCRLGVPLRQVAEPVDGPWANGVRRIATEAGITVIAGM
FTPTGDGRVTNTLIAAGPGTPNQPDAHYHKIHLYDAFGFTESRTVAPGREPVVVVVDGVRVGLTVCYDIRFPALYTELAR
RGAQLIAVCASWGSGPGKLEQWTLLARARALDSMSYVAAAGQADPGDARTGVGASSAAPTGVGGSLVASPLGEVVVSAGT
QPQLLVADIDVDNVAAARDRIAVLRNQTDFVQIDKAQSRG

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain

Homologues:

Organism=Homo sapiens, GI9910460, Length=276, Percent_Identity=32.9710144927536, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI297632348, Length=279, Percent_Identity=29.7491039426523, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI297632350, Length=279, Percent_Identity=29.7491039426523, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI5031947, Length=279, Percent_Identity=29.7491039426523, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17556280, Length=280, Percent_Identity=28.2142857142857, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6322335, Length=301, Percent_Identity=27.5747508305648, Blast_Score=99, Evalue=6e-22,
Organism=Saccharomyces cerevisiae, GI6323383, Length=283, Percent_Identity=26.1484098939929, Blast_Score=88, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21355835, Length=273, Percent_Identity=28.2051282051282, Blast_Score=104, Evalue=5e-23,
Organism=Drosophila melanogaster, GI17933642, Length=261, Percent_Identity=28.3524904214559, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y480_MYCTU (Q11146)

Other databases:

- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   C70743
- RefSeq:   NP_214994.2
- RefSeq:   NP_334907.1
- ProteinModelPortal:   Q11146
- SMR:   Q11146
- EnsemblBacteria:   EBMYCT00000001514
- EnsemblBacteria:   EBMYCT00000069635
- GeneID:   887163
- GeneID:   923850
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0498
- KEGG:   mtu:Rv0480c
- TIGR:   MT0498
- TubercuList:   Rv0480c
- GeneTree:   EBGT00050000015646
- HOGENOM:   HBG742418
- OMA:   LDGPWAN
- ProtClustDB:   CLSK881173
- InterPro:   IPR003010
- InterPro:   IPR001110
- Gene3D:   G3DSA:3.60.110.10

Pfam domain/function: PF00795 CN_hydrolase; SSF56317 Ntlse/CNhydtse

EC number: 3.5.-.- [C]

Molecular weight: Translated: 28995; Mature: 28995

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: PS50263 CN_HYDROLASE; PS01227 UPF0012

Important sites: ACT_SITE 100-100 ACT_SITE 170-170 ACT_SITE 206-206

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIALAQIRSGTDPAANLQLVGKYAGEAATAGAQLVVFPEATMCRLGVPLRQVAEPVDGP
CEEEEEEHHCCCCCCCCEEEEEECCCCHHCCCCEEEEECCCHHHHCCCCHHHHCCCCCCC
WANGVRRIATEAGITVIAGMFTPTGDGRVTNTLIAAGPGTPNQPDAHYHKIHLYDAFGFT
HHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEEEECCCC
ESRTVAPGREPVVVVVDGVRVGLTVCYDIRFPALYTELARRGAQLIAVCASWGSGPGKLE
CCCCCCCCCCCEEEEECCCEEEEEEEEECCCHHHHHHHHHCCHHEEEEEECCCCCCCCHH
QWTLLARARALDSMSYVAAAGQADPGDARTGVGASSAAPTGVGGSLVASPLGEVVVSAGT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QPQLLVADIDVDNVAAARDRIAVLRNQTDFVQIDKAQSRG
CCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
>Mature Secondary Structure
MRIALAQIRSGTDPAANLQLVGKYAGEAATAGAQLVVFPEATMCRLGVPLRQVAEPVDGP
CEEEEEEHHCCCCCCCCEEEEEECCCCHHCCCCEEEEECCCHHHHCCCCHHHHCCCCCCC
WANGVRRIATEAGITVIAGMFTPTGDGRVTNTLIAAGPGTPNQPDAHYHKIHLYDAFGFT
HHHHHHHHHHHCCEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCCCEEEEEEEEECCCC
ESRTVAPGREPVVVVVDGVRVGLTVCYDIRFPALYTELARRGAQLIAVCASWGSGPGKLE
CCCCCCCCCCCEEEEECCCEEEEEEEEECCCHHHHHHHHHCCHHEEEEEECCCCCCCCHH
QWTLLARARALDSMSYVAAAGQADPGDARTGVGASSAAPTGVGGSLVASPLGEVVVSAGT
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
QPQLLVADIDVDNVAAARDRIAVLRNQTDFVQIDKAQSRG
CCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036