Click here to switch to the map view.

The map label for this gene is fadB2

Identifier: 148660234

GI number: 148660234

Start: 560205

End: 561065

Strand: Direct

Name: fadB2

Synonym: MRA_0474

Alternate gene names: 148660234

Gene position: 560205-561065 (Clockwise)

Preceding gene: 148660233

Following gene: 148660235

Centisome position: 12.67

GC content: 64.0

Gene sequence:

>861_bases
GTGAGCGATGCGATCCAGCGGGTAGGGGTTGTCGGGGCCGGGCAGATGGGGTCCGGCATCGCCGAGGTCTCGGCTCGCGC
CGGCGTCGAAGTGACGGTGTTCGAGCCGGCCGAGGCGTTGATCACCGCGGGACGCAACCGCATCGTGAAGTCGCTGGAGC
GGGCCGTCAGCGCCGGCAAGGTAACCGAGCGCGAGCGTGACCGCGCCCTCGGCCTGTTGACCTTCACCACCGACCTCAAC
GACCTATCCGATAGGCAACTGGTGATCGAGGCCGTTGTCGAGGACGAGGCCGTCAAGTCCGAGATCTTCGCCGAGCTCGA
CCGGGTCGTCACCGATCCGGACGCGGTGCTGGCGTCGAATACCTCCAGCATCCCGATCATGAAGGTCGCCGCGGCCACCA
AGCAGCCGCAACGGGTTCTTGGCCTGCATTTCTTCAATCCGGTCCCGGTGCTGCCGCTGGTCGAGTTGGTGCGCACGCTG
GTCACCGACGAAGCCGCCGCCGCGCGCACGGAGGAGTTTGCCAGTACTGTGCTGGGCAAACAGGTCGTGCGTTGCTCCGA
CCGCTCCGGATTCGTGGTCAATGCGCTCCTGGTGCCGTATTTGCTGTCGGCGATTCGGATGGTCGAGGCCGGGTTTGCCA
CCGTCGAAGATGTCGACAAGGCCGTTGTTGCGGGGTTATCGCACCCGATGGGTCCGCTGCGGCTTTCCGATCTTGTCGGC
CTAGACACCCTCAAGCTGATCGCGGACAAGATGTTCGAAGAATTCAAAGAACCGCACTACGGGCCCCCTCCGCTGTTGCT
GCGTATGGTTGAGGCGGGCCAGTTGGGAAAGAAATCGGGTCGAGGTTTCTACACGTACTGA

Upstream 100 bases:

>100_bases
AGAGGGCCAGTTCCACTAGTCTGCCGAGCAGACGCAAAAGCACCCTTTTGCGGCGCAAAAGTGGCGCTTTTGCGTCTGCT
CGCGCATTTGAGGAGGAACA

Downstream 100 bases:

>100_bases
AGTGTATGAACGGCCCCCAGGCTTGACGCAAGGCGAGATCACAGACCGAGACGGTGTGGTTACGATCGTGTGACAGCCGT
TGCGTACATCGGGTAGTATT

Product: 3-hydroxybutyryl-CoA dehydrogenase

Products: NA

Alternate protein names: Beta-hydroxybutyryl-CoA dehydrogenase; BHBD

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSDAIQRVGVVGAGQMGSGIAEVSARAGVEVTVFEPAEALITAGRNRIVKSLERAVSAGKVTERERDRALGLLTFTTDLN
DLSDRQLVIEAVVEDEAVKSEIFAELDRVVTDPDAVLASNTSSIPIMKVAAATKQPQRVLGLHFFNPVPVLPLVELVRTL
VTDEAAAARTEEFASTVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLVG
LDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGQLGKKSGRGFYTY

Sequences:

>Translated_286_residues
MSDAIQRVGVVGAGQMGSGIAEVSARAGVEVTVFEPAEALITAGRNRIVKSLERAVSAGKVTERERDRALGLLTFTTDLN
DLSDRQLVIEAVVEDEAVKSEIFAELDRVVTDPDAVLASNTSSIPIMKVAAATKQPQRVLGLHFFNPVPVLPLVELVRTL
VTDEAAAARTEEFASTVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLVG
LDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGQLGKKSGRGFYTY
>Mature_285_residues
SDAIQRVGVVGAGQMGSGIAEVSARAGVEVTVFEPAEALITAGRNRIVKSLERAVSAGKVTERERDRALGLLTFTTDLND
LSDRQLVIEAVVEDEAVKSEIFAELDRVVTDPDAVLASNTSSIPIMKVAAATKQPQRVLGLHFFNPVPVLPLVELVRTLV
TDEAAAARTEEFASTVLGKQVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLVGL
DTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGQLGKKSGRGFYTY

Specific function: Catalyzes the NAD-dependent dehydration of beta- hydroxybutyryl-CoA to acetoacetyl-CoA in vitro at pH 10. Also catalyzes the reverse reaction albeit in a lower pH range of 5.5- 6.5. The reverse reaction is able to use NADPH as well as NADH

COG id: COG1250

COG function: function code I; 3-hydroxyacyl-CoA dehydrogenase

Gene ontology:

Cell location: Mitochondria or Peroxisomes [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyacyl-CoA dehydrogenase family

Homologues:

Organism=Homo sapiens, GI296179429, Length=290, Percent_Identity=41.0344827586207, Blast_Score=210, Evalue=9e-55,
Organism=Homo sapiens, GI296179427, Length=307, Percent_Identity=38.7622149837134, Blast_Score=201, Evalue=5e-52,
Organism=Homo sapiens, GI20127408, Length=283, Percent_Identity=32.8621908127208, Blast_Score=152, Evalue=3e-37,
Organism=Homo sapiens, GI68989263, Length=296, Percent_Identity=31.4189189189189, Blast_Score=124, Evalue=8e-29,
Organism=Homo sapiens, GI261878539, Length=296, Percent_Identity=31.4189189189189, Blast_Score=124, Evalue=9e-29,
Organism=Homo sapiens, GI115430219, Length=258, Percent_Identity=27.906976744186, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787661, Length=285, Percent_Identity=41.0526315789474, Blast_Score=186, Evalue=1e-48,
Organism=Escherichia coli, GI1788682, Length=263, Percent_Identity=34.9809885931559, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790281, Length=271, Percent_Identity=31.3653136531365, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17549919, Length=289, Percent_Identity=40.4844290657439, Blast_Score=213, Evalue=9e-56,
Organism=Caenorhabditis elegans, GI17553560, Length=288, Percent_Identity=37.8472222222222, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17563036, Length=293, Percent_Identity=38.9078498293515, Blast_Score=179, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17508951, Length=288, Percent_Identity=32.2916666666667, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17508953, Length=288, Percent_Identity=32.2916666666667, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI25144276, Length=288, Percent_Identity=32.2916666666667, Blast_Score=144, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17558304, Length=283, Percent_Identity=31.095406360424, Blast_Score=141, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI71985923, Length=284, Percent_Identity=28.8732394366197, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71985930, Length=287, Percent_Identity=27.5261324041812, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI193203623, Length=161, Percent_Identity=33.5403726708075, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI115533614, Length=161, Percent_Identity=33.5403726708075, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24583077, Length=283, Percent_Identity=35.3356890459364, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24583079, Length=283, Percent_Identity=35.3356890459364, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19921000, Length=283, Percent_Identity=35.3356890459364, Blast_Score=152, Evalue=2e-37,
Organism=Drosophila melanogaster, GI45549573, Length=278, Percent_Identity=28.0575539568345, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20129993, Length=248, Percent_Identity=27.4193548387097, Blast_Score=78, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FADB2_MYCTU (O53753)

Other databases:

- EMBL:   AE000516
- EMBL:   BX842573
- PIR:   H70828
- RefSeq:   NP_214982.1
- RefSeq:   NP_334894.1
- HSSP:   P00348
- ProteinModelPortal:   O53753
- SMR:   O53753
- EnsemblBacteria:   EBMYCT00000002940
- EnsemblBacteria:   EBMYCT00000069338
- GeneID:   886288
- GeneID:   923831
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0484
- KEGG:   mtu:Rv0468
- TIGR:   MT0484
- TubercuList:   Rv0468
- GeneTree:   EBGT00050000015509
- HOGENOM:   HBG691737
- OMA:   QVKTEIF
- ProtClustDB:   PRK07819
- GO:   GO:0005829
- InterPro:   IPR022694
- InterPro:   IPR006176
- InterPro:   IPR006108
- InterPro:   IPR008927
- InterPro:   IPR013328
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PIRSF:   PIRSF000105

Pfam domain/function: PF00725 3HCDH; PF02737 3HCDH_N; SSF48179 6DGDH_C_like

EC number: =1.1.1.157

Molecular weight: Translated: 30729; Mature: 30598

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00067 3HCDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAIQRVGVVGAGQMGSGIAEVSARAGVEVTVFEPAEALITAGRNRIVKSLERAVSAGK
CCHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
VTERERDRALGLLTFTTDLNDLSDRQLVIEAVVEDEAVKSEIFAELDRVVTDPDAVLASN
CHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
TSSIPIMKVAAATKQPQRVLGLHFFNPVPVLPLVELVRTLVTDEAAAARTEEFASTVLGK
CCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHC
LDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGQLGKKSGRGFYTY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDAIQRVGVVGAGQMGSGIAEVSARAGVEVTVFEPAEALITAGRNRIVKSLERAVSAGK
CHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
VTERERDRALGLLTFTTDLNDLSDRQLVIEAVVEDEAVKSEIFAELDRVVTDPDAVLASN
CHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCC
TSSIPIMKVAAATKQPQRVLGLHFFNPVPVLPLVELVRTLVTDEAAAARTEEFASTVLGK
CCCCCHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCHHHHHHHC
LDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGQLGKKSGRGFYTY
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036