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The map label for this gene is metZ [H]

Identifier: 148660156

GI number: 148660156

Start: 471320

End: 472540

Strand: Direct

Name: metZ [H]

Synonym: MRA_0398

Alternate gene names: 148660156

Gene position: 471320-472540 (Clockwise)

Preceding gene: 148660155

Following gene: 148660158

Centisome position: 10.66

GC content: 65.27

Gene sequence:

>1221_bases
ATGACCGACGAGTCTTCGGTCCGCACCCCGAAGGCGCTGCCCGACGGCGTCAGCCAGGCCACCGTCGGGGTGCGCGGCGG
GATGTTGCGGTCGGGGTTCGAAGAGACCGCCGAGGCGATGTACCTGACGTCCGGATATGTCTACGGCTCGGCGGCGGTTG
CCGAGAAGTCGTTCGCTGGCGAGCTGGACCACTATGTGTACTCCCGCTACGGCAACCCAACGGTGTCGGTGTTCGAGGAG
CGGCTGCGGCTGATCGAGGGTGCCCCGGCGGCGTTCGCCACCGCCAGTGGCATGGCCGCGGTATTCACCTCGCTGGGCGC
GCTGCTGGGTGCCGGAGACCGACTGGTTGCCGCGCGCAGCCTGTTTGGCTCGTGTTTCGTGGTGTGCAGCGAGATCCTGC
CGCGCTGGGGGGTGCAGACCGTCTTCGTCGACGGTGACGACCTCTCGCAATGGGAGCGGGCGCTTTCGGTACCCACGCAG
GCCGTGTTCTTCGAGACGCCGTCCAATCCCATGCAGTCGCTGGTGGATATCGCTGCGGTGACCGAGCTGGCACATGCCGC
GGGTGCAAAAGTGGTGCTGGACAACGTATTTGCCACACCGCTACTGCAGCAGGGCTTTCCGCTGGGGGTCGACGTGGTGG
TGTACTCGGGCACCAAGCACATCGACGGTCAGGGTCGGGTGCTGGGCGGGGCCATACTCGGTGACCGGGAGTACATCGAC
GGTCCGGTGCAAAAGCTGATGCGCCACACCGGTCCGGCGATGAGTGCGTTCAACGCCTGGGTACTGTTGAAAGGCCTTGA
GACGCTGGCTATTCGGGTGCAACACAGCAATGCCTCGGCGCAGCGGATCGCGGAGTTCCTCAACGGCCATCCCTCGGTTC
GGTGGGTGCGTTACCCGTACCTGCCGTCGCACCCACAATATGACCTGGCCAAGCGTCAGATGTCCGGTGGCGGAACCGTC
GTTACCTTCGCACTCGACTGCCCGGAGGATGTTGCCAAACAGCGGGCCTTCGAGGTGCTCGACAAGATGCGGCTGATCGA
CATCTCCAACAACCTCGGCGACGCCAAATCGCTTGTCACCCACCCCGCCACCACGACGCACCGGGCGATGGGCCCGGAGG
GCCGGGCCGCGATCGGGCTCGGTGACGGTGTGGTCCGCATCTCGGTTGGGTTGGAAGACACCGACGACCTGATTGCCGAT
ATCGATCGGGCGTTGAGCTAA

Upstream 100 bases:

>100_bases
CGGCCTATAACGTGCTGGACGGCTTCGAAGGGCATCTCGACGCTGAGGGTCATCGAGGCGCAACGGGCTGGCGGGCGGTG
GGACTGCCGTGGAGACAGGG

Downstream 100 bases:

>100_bases
CCCGCTGCCTCTTGCTCGGCGTGCTCGGCCTGTTCGGCGGCTGCCAGCGCTCCTTGTGCCTGCTGTTCCATCAAGGTCAT
CACTAACCTGGCGTAGATCA

Product: O-succinylhomoserine sulfhydrylase

Products: cystathionine; succinate

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 406; Mature: 405

Protein sequence:

>406_residues
MTDESSVRTPKALPDGVSQATVGVRGGMLRSGFEETAEAMYLTSGYVYGSAAVAEKSFAGELDHYVYSRYGNPTVSVFEE
RLRLIEGAPAAFATASGMAAVFTSLGALLGAGDRLVAARSLFGSCFVVCSEILPRWGVQTVFVDGDDLSQWERALSVPTQ
AVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGGAILGDREYID
GPVQKLMRHTGPAMSAFNAWVLLKGLETLAIRVQHSNASAQRIAEFLNGHPSVRWVRYPYLPSHPQYDLAKRQMSGGGTV
VTFALDCPEDVAKQRAFEVLDKMRLIDISNNLGDAKSLVTHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEDTDDLIAD
IDRALS

Sequences:

>Translated_406_residues
MTDESSVRTPKALPDGVSQATVGVRGGMLRSGFEETAEAMYLTSGYVYGSAAVAEKSFAGELDHYVYSRYGNPTVSVFEE
RLRLIEGAPAAFATASGMAAVFTSLGALLGAGDRLVAARSLFGSCFVVCSEILPRWGVQTVFVDGDDLSQWERALSVPTQ
AVFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGGAILGDREYID
GPVQKLMRHTGPAMSAFNAWVLLKGLETLAIRVQHSNASAQRIAEFLNGHPSVRWVRYPYLPSHPQYDLAKRQMSGGGTV
VTFALDCPEDVAKQRAFEVLDKMRLIDISNNLGDAKSLVTHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEDTDDLIAD
IDRALS
>Mature_405_residues
TDESSVRTPKALPDGVSQATVGVRGGMLRSGFEETAEAMYLTSGYVYGSAAVAEKSFAGELDHYVYSRYGNPTVSVFEER
LRLIEGAPAAFATASGMAAVFTSLGALLGAGDRLVAARSLFGSCFVVCSEILPRWGVQTVFVDGDDLSQWERALSVPTQA
VFFETPSNPMQSLVDIAAVTELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGGAILGDREYIDG
PVQKLMRHTGPAMSAFNAWVLLKGLETLAIRVQHSNASAQRIAEFLNGHPSVRWVRYPYLPSHPQYDLAKRQMSGGGTVV
TFALDCPEDVAKQRAFEVLDKMRLIDISNNLGDAKSLVTHPATTTHRAMGPEGRAAIGLGDGVVRISVGLEDTDDLIADI
DRALS

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=332, Percent_Identity=32.5301204819277, Blast_Score=191, Evalue=8e-49,
Organism=Homo sapiens, GI299473759, Length=332, Percent_Identity=28.9156626506024, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI25453487, Length=331, Percent_Identity=27.7945619335347, Blast_Score=138, Evalue=1e-32,
Organism=Escherichia coli, GI1790375, Length=388, Percent_Identity=33.5051546391753, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1789383, Length=349, Percent_Identity=27.2206303724928, Blast_Score=127, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17560056, Length=336, Percent_Identity=34.5238095238095, Blast_Score=201, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI17538055, Length=336, Percent_Identity=33.9285714285714, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71980708, Length=347, Percent_Identity=30.835734870317, Blast_Score=175, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=407, Percent_Identity=32.1867321867322, Blast_Score=200, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6319307, Length=363, Percent_Identity=34.7107438016529, Blast_Score=186, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6321254, Length=360, Percent_Identity=25.8333333333333, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321170, Length=263, Percent_Identity=26.9961977186312, Blast_Score=107, Evalue=4e-24,
Organism=Saccharomyces cerevisiae, GI6321904, Length=347, Percent_Identity=25.3602305475504, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI19922554, Length=348, Percent_Identity=32.7586206896552, Blast_Score=202, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48

Molecular weight: Translated: 43346; Mature: 43214

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDESSVRTPKALPDGVSQATVGVRGGMLRSGFEETAEAMYLTSGYVYGSAAVAEKSFAG
CCCCCCCCCCCCCCCCCHHHHHHHCCCHHHCCHHHHHHHEEEECCEEECCHHHHHHHHHH
ELDHYVYSRYGNPTVSVFEERLRLIEGAPAAFATASGMAAVFTSLGALLGAGDRLVAARS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LFGSCFVVCSEILPRWGVQTVFVDGDDLSQWERALSVPTQAVFFETPSNPMQSLVDIAAV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
TELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGGAILGDREYID
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCEEEEEEEECCHHHCC
GPVQKLMRHTGPAMSAFNAWVLLKGLETLAIRVQHSNASAQRIAEFLNGHPSVRWVRYPY
HHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECC
LPSHPQYDLAKRQMSGGGTVVTFALDCPEDVAKQRAFEVLDKMRLIDISNNLGDAKSLVT
CCCCCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHC
HPATTTHRAMGPEGRAAIGLGDGVVRISVGLEDTDDLIADIDRALS
CCCCCCHHCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TDESSVRTPKALPDGVSQATVGVRGGMLRSGFEETAEAMYLTSGYVYGSAAVAEKSFAG
CCCCCCCCCCCCCCCCHHHHHHHCCCHHHCCHHHHHHHEEEECCEEECCHHHHHHHHHH
ELDHYVYSRYGNPTVSVFEERLRLIEGAPAAFATASGMAAVFTSLGALLGAGDRLVAARS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
LFGSCFVVCSEILPRWGVQTVFVDGDDLSQWERALSVPTQAVFFETPSNPMQSLVDIAAV
HHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHH
TELAHAAGAKVVLDNVFATPLLQQGFPLGVDVVVYSGTKHIDGQGRVLGGAILGDREYID
HHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCEEEEEEEECCHHHCC
GPVQKLMRHTGPAMSAFNAWVLLKGLETLAIRVQHSNASAQRIAEFLNGHPSVRWVRYPY
HHHHHHHHHCCHHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCCCCEEEEEECC
LPSHPQYDLAKRQMSGGGTVVTFALDCPEDVAKQRAFEVLDKMRLIDISNNLGDAKSLVT
CCCCCCHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHEEEEECCCCCCHHHHHC
HPATTTHRAMGPEGRAAIGLGDGVVRISVGLEDTDDLIADIDRALS
CCCCCCHHCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; L-cysteine

Specific reaction: O-succinyl-L-homoserine + L-cysteine = cystathionine + succinate

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]