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The map label for this gene is yeiE [C]

Identifier: 148660143

GI number: 148660143

Start: 455784

End: 456749

Strand: Direct

Name: yeiE [C]

Synonym: MRA_0386

Alternate gene names: 148660143

Gene position: 455784-456749 (Clockwise)

Preceding gene: 148660133

Following gene: 148660144

Centisome position: 10.31

GC content: 67.39

Gene sequence:

>966_bases
ATGACCCCGGCTCAACTTCGGGCCTATTCGGCGGTGGTTCGCCTGGGCTCGGTACGGGCGGCCGCCGCGGAACTCGGTCT
TTCCGACGCCGGAGTCTCCATGCACGTCGCGGCGCTGCGCAAGGAACTCGACGACCCGCTGTTTACCAGGACCGGTGCCG
GGCTGGCGTTCACGCCCGGCGGGCTGCGGCTGGCCAGCCGCGCGGTCGAAATCCTGGGCCTGCAACAACAAACCGCGATC
GAGGTCACCGAGGCCGCCCACGGGCGTCGGTTGCTGCGCATCGCCGCCTCCAGCGCCTTCGCCGAACACGCCGCGCCGGG
CCTGATCGAGCTCTTCTCGTCTCGGGCCGACGACCTTTCGGTCGAGTTGAGCGTGCATCCCACCAGCCGGTTCCGCGAAC
TGATCTGCTCGCGCGCCGTCGACATCGCGATCGGCCCGGCCAGTGAGAGCTCGATCGGTTCCGACGGCTCGATCTTTCTA
CGGCCCTTCCTGAAGTATCAGATCATCACCGTCGTCGCGCCGAATAGCCCACTGGCCGCAGGCATTCCGATGCCCGCGCT
GTTGCGTCACCAGCAATGGATGTTGGGTCCGTCCGCCGGCAGCGTAGATGGTGAGATCGCAACCATGTTGCGCGGCTTGG
CGATTCCGGAGTCCCAGCAACGGATCTTCCAGAGCGATGCCGCCGCGCTGGAGGAGGTCATGCGCGTCGGGGGCGCCACG
CTGGCCATTGGCTTTGCGGTCGCCAAGGATCTTGCCGCCGGACGGTTGGTGCACGTGACCGGTCCTGGGCTGGATCGCGC
CGGCGAGTGGTGTGTGGCGACATTGGCGCCTTCGGCCCGCCAACCCGCCGTCTCCGAGCTTGTTGGCTTCATCAGCACCC
CGAGGTGTATTCAGGCGATGATCCCGGGTAGCGGGGTCGGGGTGACGCGGTTCCGCCCAAAGGTCCACGTCACCCTGTGG
AGCTAG

Upstream 100 bases:

>100_bases
TTCGCACCACCGTCGCGACCCCCGCGGTGTCACCGGCGGCCCAGATCGCCATCAGCTCGGCAAGCACTTCACGCACGCTT
CCCAAAGTAGGCTTCAGTGC

Downstream 100 bases:

>100_bases
CTACTTCGACTTGAAAGGCTCGGCGCGCCGGTCCGCCCGTTGACGGGGCCCGGCTGCGAGGATTAGCCAGTTCCCTTGTC
GCACAGGAGCGTTGAGGCTA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTPAQLRAYSAVVRLGSVRAAAAELGLSDAGVSMHVAALRKELDDPLFTRTGAGLAFTPGGLRLASRAVEILGLQQQTAI
EVTEAAHGRRLLRIAASSAFAEHAAPGLIELFSSRADDLSVELSVHPTSRFRELICSRAVDIAIGPASESSIGSDGSIFL
RPFLKYQIITVVAPNSPLAAGIPMPALLRHQQWMLGPSAGSVDGEIATMLRGLAIPESQQRIFQSDAAALEEVMRVGGAT
LAIGFAVAKDLAAGRLVHVTGPGLDRAGEWCVATLAPSARQPAVSELVGFISTPRCIQAMIPGSGVGVTRFRPKVHVTLW
S

Sequences:

>Translated_321_residues
MTPAQLRAYSAVVRLGSVRAAAAELGLSDAGVSMHVAALRKELDDPLFTRTGAGLAFTPGGLRLASRAVEILGLQQQTAI
EVTEAAHGRRLLRIAASSAFAEHAAPGLIELFSSRADDLSVELSVHPTSRFRELICSRAVDIAIGPASESSIGSDGSIFL
RPFLKYQIITVVAPNSPLAAGIPMPALLRHQQWMLGPSAGSVDGEIATMLRGLAIPESQQRIFQSDAAALEEVMRVGGAT
LAIGFAVAKDLAAGRLVHVTGPGLDRAGEWCVATLAPSARQPAVSELVGFISTPRCIQAMIPGSGVGVTRFRPKVHVTLW
S
>Mature_320_residues
TPAQLRAYSAVVRLGSVRAAAAELGLSDAGVSMHVAALRKELDDPLFTRTGAGLAFTPGGLRLASRAVEILGLQQQTAIE
VTEAAHGRRLLRIAASSAFAEHAAPGLIELFSSRADDLSVELSVHPTSRFRELICSRAVDIAIGPASESSIGSDGSIFLR
PFLKYQIITVVAPNSPLAAGIPMPALLRHQQWMLGPSAGSVDGEIATMLRGLAIPESQQRIFQSDAAALEEVMRVGGATL
AIGFAVAKDLAAGRLVHVTGPGLDRAGEWCVATLAPSARQPAVSELVGFISTPRCIQAMIPGSGVGVTRFRPKVHVTLWS

Specific function: Unknown

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1788481, Length=266, Percent_Identity=27.8195488721804, Blast_Score=69, Evalue=3e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): Y377_MYCTU (O53712)

Other databases:

- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   D70833
- RefSeq:   NP_214891.1
- RefSeq:   NP_334799.1
- ProteinModelPortal:   O53712
- SMR:   O53712
- EnsemblBacteria:   EBMYCT00000000816
- EnsemblBacteria:   EBMYCT00000070112
- GeneID:   886452
- GeneID:   923599
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0391
- KEGG:   mtu:Rv0377
- TIGR:   MT0391
- TubercuList:   Rv0377
- GeneTree:   EBGT00050000015804
- HOGENOM:   HBG323665
- OMA:   ITTPRCI
- ProtClustDB:   CLSK790477
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 33637; Mature: 33506

Theoretical pI: Translated: 8.52; Mature: 8.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAQLRAYSAVVRLGSVRAAAAELGLSDAGVSMHVAALRKELDDPLFTRTGAGLAFTPG
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCEEECCC
GLRLASRAVEILGLQQQTAIEVTEAAHGRRLLRIAASSAFAEHAAPGLIELFSSRADDLS
CHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEE
VELSVHPTSRFRELICSRAVDIAIGPASESSIGSDGSIFLRPFLKYQIITVVAPNSPLAA
EEEEECCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHEEECCEEEEEEEEECCCCCCC
GIPMPALLRHQQWMLGPSAGSVDGEIATMLRGLAIPESQQRIFQSDAAALEEVMRVGGAT
CCCCHHHHHCHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHH
LAIGFAVAKDLAAGRLVHVTGPGLDRAGEWCVATLAPSARQPAVSELVGFISTPRCIQAM
HHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHH
IPGSGVGVTRFRPKVHVTLWS
CCCCCCCEEEECCEEEEEEEC
>Mature Secondary Structure 
TPAQLRAYSAVVRLGSVRAAAAELGLSDAGVSMHVAALRKELDDPLFTRTGAGLAFTPG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCCCCCEEECCC
GLRLASRAVEILGLQQQTAIEVTEAAHGRRLLRIAASSAFAEHAAPGLIELFSSRADDLS
CHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCEE
VELSVHPTSRFRELICSRAVDIAIGPASESSIGSDGSIFLRPFLKYQIITVVAPNSPLAA
EEEEECCHHHHHHHHHHHHHEEEECCCCCCCCCCCCCHHEEECCEEEEEEEEECCCCCCC
GIPMPALLRHQQWMLGPSAGSVDGEIATMLRGLAIPESQQRIFQSDAAALEEVMRVGGAT
CCCCHHHHHCHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHH
LAIGFAVAKDLAAGRLVHVTGPGLDRAGEWCVATLAPSARQPAVSELVGFISTPRCIQAM
HHHHHHHHHHHHCCEEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCHHHHHH
IPGSGVGVTRFRPKVHVTLWS
CCCCCCCEEEECCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036