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The map label for this gene is purA [H]

Identifier: 148660123

GI number: 148660123

Start: 436834

End: 438132

Strand: Reverse

Name: purA [H]

Synonym: MRA_0366

Alternate gene names: 148660123

Gene position: 438132-436834 (Counterclockwise)

Preceding gene: 148660126

Following gene: 148660122

Centisome position: 9.91

GC content: 65.13

Gene sequence:

>1299_bases
ATGCCGGCGATCGTCCTCATCGGCGCCCAATGGGGCGACGAGGGCAAGGGTAAGGCCACCGACCTGTTGGGTGGCCGCGT
GCAGTGGGTGGTGCGCTATCAGGGGGGCAACAACGCCGGGCACACCGTCGTCTTGCCTACCGGCGAGAATTTCGCATTGC
ATCTCATCCCGTCGGGTGTGCTGACGCCCGGGGTCACCAACGTTATCGGCAACGGCGTGGTGATCGATCCCGGTGTCCTG
CTCAATGAGCTGCGGGGACTGCAAGACCGCGGCGTGGACACCGCCAAGCTGTTGATCTCCGCCGACGCCCATCTGCTGAT
GCCTTACCACATTGCTATCGACAAGGTCACCGAGCGCTATATGGGCAGCAAGAAGATCGGCACCACCGGCCGTGGTATCG
GGCCGTGCTACCAGGACAAGATCGCCCGTATTGGGATCCGGGTCGCCGATGTGCTGGACCCGGAACAATTGACCCACAAG
GTCGAGGCCGCGTGCGAGTTCAAGAACCAAGTGCTGGTCAAGATCTACAACCGCAAGGCCCTGGACCCCGCGCAGGTGGT
CGACGCGCTGCTGGAGCAGGCCGAGGGTTTCAAGCATCGCATCGCCGACACCCGGCTGCTACTCAATGCTGCCCTGGAGG
CCGGCGAAACGGTGCTGCTGGAAGGCTCTCAAGGCACCCTGCTCGATGTCGACCACGGCACCTACCCGTATGTGACATCG
TCGAATCCGACGGCGGGTGGCGCGGCCGTGGGCTCTGGTATCGGCCCGACCCGCATCGGCACCGTGCTGGGGATTCTCAA
GGCCTACACCACTCGGGTGGGCTCCGGCCCGTTCCCCACCGAGCTGTTCGACGAACACGGCGAATACCTGTCCAAGACTG
GCCGCGAGTTCGGTGTGACCACCGGCCGGCGCCGGCGCTGCGGCTGGTTCGACGCCGTCATTGCCCGCTACGCCGCCCGG
GTCAACGGCATCACCGACTACTTCCTGACCAAGCTCGACGTGCTGTCCAGCCTGGAATCGGTGCCGGTCTGCGTCGGATA
TGAGATCGACGGCCGGCGCACCCGCGATATGCCGATGACCCAGCGGGATCTTTGCCGCGCCAAGCCGGTTTACGAGGAGC
TGCCGGGCTGGTGGGAAGACATCTCCGGGGCGCGCGAGTTCGACGACCTGCCCGCCAAAGCGCGTGATTATGTGCTGCGA
CTGGAACAGCTTGCCGGAGCACCGGTTTCGTGCATTGGAGTCGGTCCGGGGCGGGAACAGACCATCGTGCGCCGGGACGT
CCTGCAGGATCGCCCGTGA

Upstream 100 bases:

>100_bases
CCGTATACATGACAGCTCCTCGCAACCTGCTGGGTAGGGTAGGTCACCGGCTGGACCACAATAAGCGGGCATAGCCTGCG
AGACCAGGTGGGAGCAACTC

Downstream 100 bases:

>100_bases
CAGATGCGTCAGTGCACCCCGACGAACTCGATCCCGAGTATCACCACCACGGTGGATTTCCGGAGTACGGTCCGGCCAGT
CCCGGTGCGGGGTTCGGCCA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGVLTPGVTNVIGNGVVIDPGVL
LNELRGLQDRGVDTAKLLISADAHLLMPYHIAIDKVTERYMGSKKIGTTGRGIGPCYQDKIARIGIRVADVLDPEQLTHK
VEAACEFKNQVLVKIYNRKALDPAQVVDALLEQAEGFKHRIADTRLLLNAALEAGETVLLEGSQGTLLDVDHGTYPYVTS
SNPTAGGAAVGSGIGPTRIGTVLGILKAYTTRVGSGPFPTELFDEHGEYLSKTGREFGVTTGRRRRCGWFDAVIARYAAR
VNGITDYFLTKLDVLSSLESVPVCVGYEIDGRRTRDMPMTQRDLCRAKPVYEELPGWWEDISGAREFDDLPAKARDYVLR
LEQLAGAPVSCIGVGPGREQTIVRRDVLQDRP

Sequences:

>Translated_432_residues
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGVLTPGVTNVIGNGVVIDPGVL
LNELRGLQDRGVDTAKLLISADAHLLMPYHIAIDKVTERYMGSKKIGTTGRGIGPCYQDKIARIGIRVADVLDPEQLTHK
VEAACEFKNQVLVKIYNRKALDPAQVVDALLEQAEGFKHRIADTRLLLNAALEAGETVLLEGSQGTLLDVDHGTYPYVTS
SNPTAGGAAVGSGIGPTRIGTVLGILKAYTTRVGSGPFPTELFDEHGEYLSKTGREFGVTTGRRRRCGWFDAVIARYAAR
VNGITDYFLTKLDVLSSLESVPVCVGYEIDGRRTRDMPMTQRDLCRAKPVYEELPGWWEDISGAREFDDLPAKARDYVLR
LEQLAGAPVSCIGVGPGREQTIVRRDVLQDRP
>Mature_431_residues
PAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGVLTPGVTNVIGNGVVIDPGVLL
NELRGLQDRGVDTAKLLISADAHLLMPYHIAIDKVTERYMGSKKIGTTGRGIGPCYQDKIARIGIRVADVLDPEQLTHKV
EAACEFKNQVLVKIYNRKALDPAQVVDALLEQAEGFKHRIADTRLLLNAALEAGETVLLEGSQGTLLDVDHGTYPYVTSS
NPTAGGAAVGSGIGPTRIGTVLGILKAYTTRVGSGPFPTELFDEHGEYLSKTGREFGVTTGRRRRCGWFDAVIARYAARV
NGITDYFLTKLDVLSSLESVPVCVGYEIDGRRTRDMPMTQRDLCRAKPVYEELPGWWEDISGAREFDDLPAKARDYVLRL
EQLAGAPVSCIGVGPGREQTIVRRDVLQDRP

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=433, Percent_Identity=44.5727482678984, Blast_Score=331, Evalue=8e-91,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=44.1588785046729, Blast_Score=327, Evalue=1e-89,
Organism=Homo sapiens, GI40316944, Length=404, Percent_Identity=43.5643564356436, Blast_Score=296, Evalue=3e-80,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=51.0588235294118, Blast_Score=442, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI25146555, Length=433, Percent_Identity=44.1108545034642, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI25146553, Length=433, Percent_Identity=44.1108545034642, Blast_Score=348, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6324109, Length=430, Percent_Identity=41.1627906976744, Blast_Score=338, Evalue=1e-93,
Organism=Drosophila melanogaster, GI21356033, Length=426, Percent_Identity=43.8967136150235, Blast_Score=323, Evalue=2e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 46823; Mature: 46692

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGV
CCEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCC
LTPGVTNVIGNGVVIDPGVLLNELRGLQDRGVDTAKLLISADAHLLMPYHIAIDKVTERY
CCCCHHHHHCCCEEECCHHHHHHHCCHHHCCCCCEEEEEECCCEEEEHHHHHHHHHHHHH
MGSKKIGTTGRGIGPCYQDKIARIGIRVADVLDPEQLTHKVEAACEFKNQVLVKIYNRKA
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
LDPAQVVDALLEQAEGFKHRIADTRLLLNAALEAGETVLLEGSQGTLLDVDHGTYPYVTS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTAGGAAVGSGIGPTRIGTVLGILKAYTTRVGSGPFPTELFDEHGEYLSKTGREFGVT
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
TGRRRRCGWFDAVIARYAARVNGITDYFLTKLDVLSSLESVPVCVGYEIDGRRTRDMPMT
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
QRDLCRAKPVYEELPGWWEDISGAREFDDLPAKARDYVLRLEQLAGAPVSCIGVGPGREQ
HHHHHHCCCHHHHCCCHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCH
TIVRRDVLQDRP
HHHHHHHHCCCC
>Mature Secondary Structure 
PAIVLIGAQWGDEGKGKATDLLGGRVQWVVRYQGGNNAGHTVVLPTGENFALHLIPSGV
CEEEEEECCCCCCCCCCHHHHCCCEEEEEEEECCCCCCCCEEEEECCCCEEEEEECCCC
LTPGVTNVIGNGVVIDPGVLLNELRGLQDRGVDTAKLLISADAHLLMPYHIAIDKVTERY
CCCCHHHHHCCCEEECCHHHHHHHCCHHHCCCCCEEEEEECCCEEEEHHHHHHHHHHHHH
MGSKKIGTTGRGIGPCYQDKIARIGIRVADVLDPEQLTHKVEAACEFKNQVLVKIYNRKA
CCCCCCCCCCCCCCCHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCEEEEEECCCC
LDPAQVVDALLEQAEGFKHRIADTRLLLNAALEAGETVLLEGSQGTLLDVDHGTYPYVTS
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEEECCCCCCCEEEC
SNPTAGGAAVGSGIGPTRIGTVLGILKAYTTRVGSGPFPTELFDEHGEYLSKTGREFGVT
CCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
TGRRRRCGWFDAVIARYAARVNGITDYFLTKLDVLSSLESVPVCVGYEIDGRRTRDMPMT
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCC
QRDLCRAKPVYEELPGWWEDISGAREFDDLPAKARDYVLRLEQLAGAPVSCIGVGPGREQ
HHHHHHCCCHHHHCCCHHHHHCCCHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCH
TIVRRDVLQDRP
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA