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The map label for this gene is php [C]

Identifier: 148659994

GI number: 148659994

Start: 276345

End: 277325

Strand: Reverse

Name: php [C]

Synonym: MRA_0238

Alternate gene names: 148659994

Gene position: 277325-276345 (Counterclockwise)

Preceding gene: 148659998

Following gene: 148659993

Centisome position: 6.27

GC content: 65.85

Gene sequence:

>981_bases
GTGCCAGAACTAAATACCGCTCGCGGACCCATCGACACCGCTGATCTCGGCGTCACGCTGATGCACGAGCACGTCTTCAT
CATGACCACCGAGATTGCGCAGAACTACCCGGAAGCCTGGGGCGACGAGGACAAGCGGGTGGCCGGCGCCATCGCCCGGC
TAGGCGAACTCAAGGCCCGCGGCGTGGACACCATCGTCGACCTCACGGTGATCGGGCTGGGCCGATACATCCCGCGCATC
GCCCGGGTGGCCGCGGCCACCGAGCTGAACATCGTCGTGGCCACCGGCTTGTACACCTACAACGACGTCCCGTTCTACTT
CCACTACCTCGGGCCGGGCGCACAGCTGGACGGCCCGGAGATCATGACCGACATGTTCGTCCGCGACATCGAGCACGGCA
TCGCCGACACCGGCATCAAGGCGGGAATCCTCAAGTGCGCCACCGACGAACCCGGCCTCACCCCTGGTGTCGAGCGGGTG
TTGCGCGCGGTCGCCCAAGCACACAAACGCACCGGGGCGCCGATCTCCACCCACACCCACGCCGGGCTGCGGCGCGGCCT
TGACCAGCAACGCATCTTCGCCGAGGAGGGGGTGGACCTGAGCCGGGTGGTTATCGGACACTGCGGCGACAGCACCGACG
TCGGCTACCTGGAAGAGCTCATCGCCGCCGGCTCCTACCTCGGGATGGACCGGTTCGGCGTCGACGTGATCTCACCGTTT
CAGGACCGGGTGAACATCGTGGCCCGAATGTGCGAGCGCGGCCATGCCGACAAGATGGTGCTATCACACGACGCCTGCTG
CTATTTCGACGCGCTTCCCGAGGAGCTGGTGCCGGTGGCGATGCCGAATTGGCATTACCTCCACATCCACAACGACGTCA
TCCCCGCACTGAAGCAGCACGGCGTCACCGACGAGCAGCTGCACACCATGCTCGTCGACAACCCGCGCCGCATCTTCGAG
CGGCAGGGCGGCTATCAGTGA

Upstream 100 bases:

>100_bases
CAGCATAAGAATTGGCTCCCGAGCGGTTCGGTCAACGACGAGGGTGACGCTACTGCACTGCGGCCCCGCAAGACACCGAT
TGCGGCCTAGCATCAGTGAC

Downstream 100 bases:

>100_bases
GACAGCCGCGCCGGGCGAATGCCATGGGCTTGGCATTGTGCATATATATCGGCTCCTTATTGATATATACTCCGATTCAT
GGCGAAACATCTCGTCGACA

Product: phosphotriesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MPELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKARGVDTIVDLTVIGLGRYIPRI
ARVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPEIMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERV
LRAVAQAHKRTGAPISTHTHAGLRRGLDQQRIFAEEGVDLSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPF
QDRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQHGVTDEQLHTMLVDNPRRIFE
RQGGYQ

Sequences:

>Translated_326_residues
MPELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKARGVDTIVDLTVIGLGRYIPRI
ARVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPEIMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERV
LRAVAQAHKRTGAPISTHTHAGLRRGLDQQRIFAEEGVDLSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPF
QDRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQHGVTDEQLHTMLVDNPRRIFE
RQGGYQ
>Mature_325_residues
PELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKARGVDTIVDLTVIGLGRYIPRIA
RVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPEIMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERVL
RAVAQAHKRTGAPISTHTHAGLRRGLDQQRIFAEEGVDLSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPFQ
DRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQHGVTDEQLHTMLVDNPRRIFER
QGGYQ

Specific function: Its Real Enzymatic Activity Is Not Yet Known. It Was Tested For General Esterase, Aminopeptidase, Sulfatase, Phosphatase, Carbonic Anhydrase, Phosphodiesterase, And Phosphotriesterase Activities With The Following Substrates: P- Nitrophenyl Acetate, L-Ala

COG id: COG1735

COG function: function code R; Predicted metal-dependent hydrolase with the TIM-barrel fold

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphotriesterase family

Homologues:

Organism=Homo sapiens, GI20070186, Length=352, Percent_Identity=24.7159090909091, Blast_Score=86, Evalue=5e-17,
Organism=Homo sapiens, GI47933341, Length=352, Percent_Identity=24.7159090909091, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1789780, Length=305, Percent_Identity=30.8196721311475, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24645325, Length=360, Percent_Identity=23.3333333333333, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PHP_MYCTU (P96413)

Other databases:

- EMBL:   BX842572
- EMBL:   AE000516
- PIR:   D70962
- RefSeq:   NP_214744.1
- RefSeq:   NP_334647.1
- ProteinModelPortal:   P96413
- SMR:   P96413
- EnsemblBacteria:   EBMYCT00000003079
- EnsemblBacteria:   EBMYCT00000069988
- GeneID:   886705
- GeneID:   923138
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0240
- KEGG:   mtu:Rv0230c
- TIGR:   MT0240
- TubercuList:   Rv0230c
- GeneTree:   EBGT00050000018157
- HOGENOM:   HBG732614
- OMA:   EPIMMKN
- ProtClustDB:   CLSK790368
- InterPro:   IPR017947
- InterPro:   IPR001559
- PANTHER:   PTHR10819

Pfam domain/function: PF02126 PTE

EC number: NA

Molecular weight: Translated: 35886; Mature: 35755

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS01322 PHOSPHOTRIESTERASE_1; PS51347 PHOSPHOTRIESTERASE_2; PS00962 RIBOSOMAL_S2_1 ; PS01323 PHOSPHOTRIESTERASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKAR
CCCCCCCCCCCCHHHCCCEEECCEEEEEEHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHC
GVDTIVDLTVIGLGRYIPRIARVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECEEEECCCCEEEEECCCCCCCCCHH
IMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERVLRAVAQAHKRTGAPISTHTH
HHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
AGLRRGLDQQRIFAEEGVDLSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPF
HHHHHCCCHHHHHHHCCCCHHHHHEECCCCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHH
QDRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQH
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHCCHHHHCCCCCCCEEEEEECCHHHHHHHHC
GVTDEQLHTMLVDNPRRIFERQGGYQ
CCCHHHHHHHHHCCHHHHHHHCCCCC
>Mature Secondary Structure 
PELNTARGPIDTADLGVTLMHEHVFIMTTEIAQNYPEAWGDEDKRVAGAIARLGELKAR
CCCCCCCCCCCHHHCCCEEECCEEEEEEHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHC
GVDTIVDLTVIGLGRYIPRIARVAAATELNIVVATGLYTYNDVPFYFHYLGPGAQLDGPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECEEEECCCCEEEEECCCCCCCCCHH
IMTDMFVRDIEHGIADTGIKAGILKCATDEPGLTPGVERVLRAVAQAHKRTGAPISTHTH
HHHHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHH
AGLRRGLDQQRIFAEEGVDLSRVVIGHCGDSTDVGYLEELIAAGSYLGMDRFGVDVISPF
HHHHHCCCHHHHHHHCCCCHHHHHEECCCCCCCHHHHHHHHHCCCCCCHHHHCCHHHHHH
QDRVNIVARMCERGHADKMVLSHDACCYFDALPEELVPVAMPNWHYLHIHNDVIPALKQH
HHHHHHHHHHHHCCCCCCEEECCCCHHHHHCCHHHHCCCCCCCEEEEEECCHHHHHHHHC
GVTDEQLHTMLVDNPRRIFERQGGYQ
CCCHHHHHHHHHCCHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036