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The map label for this gene is pckA [H]

Identifier: 148659975

GI number: 148659975

Start: 253144

End: 254964

Strand: Direct

Name: pckA [H]

Synonym: MRA_0219

Alternate gene names: 148659975

Gene position: 253144-254964 (Clockwise)

Preceding gene: 148659974

Following gene: 148659978

Centisome position: 5.73

GC content: 64.09

Gene sequence:

>1821_bases
ATGACCTCAGCGACCATCCCCGGTCTGGATACCGCGCCGACGAATCACCAGGGGTTGCTGTCCTGGGTCGAAGAGGTCGC
CGAGCTCACCCAGCCGGACCGGGTGGTCTTCACTGACGGCTCGGAAGAAGAGTTCCAGCGGCTCTGCGATCAGCTAGTCG
AGGCCGGCACGTTCATCAGGCTCAACCCCGAGAAGCACAAGAACTCCTACCTGGCATTGTCGGATCCGTCCGATGTCGCG
CGGGTGGAGTCGCGGACGTACATCTGCTCGGCGAAAGAGATCGACGCCGGCCCCACCAACAACTGGATGGATCCCGGCGA
AATGCGGTCCATCATGAAAGACCTGTACCGGGGTTGCATGCGCGGGCGCACCATGTATGTGGTGCCGTTCTGTATGGGAC
CGCTGGGCGCCGAGGACCCCAAACTTGGTGTGGAGATCACCGACTCCGAGTACGTCGTCGTCTCCATGCGCACCATGACC
CGGATGGGCAAGGCCGCGCTGGAGAAAATGGGCGACGACGGTTTCTTTGTCAAGGCGCTGCACTCGGTCGGCGCGCCGCT
GGAACCGGGCCAAAAGGACGTGGCCTGGCCCTGCAGCGAAACCAAGTACATCACCCACTTCCCGGAGACCCGGGAGATCT
GGAGCTACGGCTCGGGCTACGGCGGCAACGCGTTGCTGGGCAAGAAGTGCTACTCACTGCGTATCGCGTCGGCGATGGCC
CACGATGAGGGCTGGCTGGCCGAGCACATGCTGATCCTCAAGCTGATTTCGCCGGAGAACAAGGCTTACTACTTCGCGGC
CGCATTCCCGTCGGCGTGTGGCAAGACCAACCTGGCGATGCTGCAGCCAACCATCCCCGGCTGGCGTGCGGAGACACTCG
GAGACGACATCGCATGGATGCGATTTGGCAAGGACGGTCGCCTGTACGCCGTCAACCCGGAATTCGGCTTCTTCGGGGTG
GCGCCGGGCACCAACTGGAAGTCGAACCCTAACGCCATGCGCACCATTGCCGCCGGCAACACGGTGTTCACCAATGTCGC
ACTCACCGACGACGGCGACGTGTGGTGGGAGGGCCTGGAAGGCGACCCGCAGCACCTGATCGACTGGAAGGGCAACGACT
GGTACTTCCGCGAGACGGAAACCAATGCGGCACACCCGAACTCCCGGTACTGCACACCGATGTCGCAGTGCCCGATCCTG
GCCCCCGAGTGGGATGACCCGCAGGGCGTCCCGATCTCGGGGATCCTGTTCGGCGGCCGCCGCAAGACCACGGTTCCGCT
GGTCACCGAGGCGCGCGACTGGCAGCACGGGGTGTTCATCGGTGCGACCCTGGGTAGCGAGCAGACCGCCGCGGCCGAGG
GCAAGGTCGGCAATGTGCGCCGCGACCCGATGGCCATGCTGCCGTTTTTGGGCTACAACGTTGGGGACTACTTCCAGCAC
TGGATCAACCTGGGCAAGCACGCCGATGAGTCCAAGCTGCCCAAGGTGTTCTTCGTCAACTGGTTCCGTCGCGGTGACGA
CGGTCGCTTCCTGTGGCCGGGCTTCGGCGAGAACAGCCGGGTGCTGAAGTGGATCGTCGATCGCATCGAGCACAAGGCCG
GCGGTGCGACCACCCCGATCGGCACCGTTCCCGCCGTGGAGGACTTGGACCTGGACGGACTGGACGTCGACGCCGCCGAT
GTAGCCGCGGCGCTGGCAGTCGATGCCGATGAATGGCGTCAGGAACTGCCGCTGATCGAAGAATGGCTGCAGTTCGTCGG
CGAGAAGCTGCCGACCGGTGTCAAAGATGAGTTCGACGCCCTGAAGGAGCGCCTAGGTTAG

Upstream 100 bases:

>100_bases
TGTCTCACTAGGTGAGCGGCCGTATCAGCCTCGCCACCAAGACGGGCATACCTAACCCATACGTAACCGCGAGCACCCGA
TAACTACGCAGGAGAATTCG

Downstream 100 bases:

>100_bases
GGCGAGCAGACGCATAAGCCCCCGCACGCACGGCGTGTCGAGGGCTTTAGTGTCTGCTCGCGCTCGTTAGCGGCGGGCAC
GCACAAGTTCTTCGACAGCG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 606; Mature: 605

Protein sequence:

>606_residues
MTSATIPGLDTAPTNHQGLLSWVEEVAELTQPDRVVFTDGSEEEFQRLCDQLVEAGTFIRLNPEKHKNSYLALSDPSDVA
RVESRTYICSAKEIDAGPTNNWMDPGEMRSIMKDLYRGCMRGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRTMT
RMGKAALEKMGDDGFFVKALHSVGAPLEPGQKDVAWPCSETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGV
APGTNWKSNPNAMRTIAAGNTVFTNVALTDDGDVWWEGLEGDPQHLIDWKGNDWYFRETETNAAHPNSRYCTPMSQCPIL
APEWDDPQGVPISGILFGGRRKTTVPLVTEARDWQHGVFIGATLGSEQTAAAEGKVGNVRRDPMAMLPFLGYNVGDYFQH
WINLGKHADESKLPKVFFVNWFRRGDDGRFLWPGFGENSRVLKWIVDRIEHKAGGATTPIGTVPAVEDLDLDGLDVDAAD
VAAALAVDADEWRQELPLIEEWLQFVGEKLPTGVKDEFDALKERLG

Sequences:

>Translated_606_residues
MTSATIPGLDTAPTNHQGLLSWVEEVAELTQPDRVVFTDGSEEEFQRLCDQLVEAGTFIRLNPEKHKNSYLALSDPSDVA
RVESRTYICSAKEIDAGPTNNWMDPGEMRSIMKDLYRGCMRGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRTMT
RMGKAALEKMGDDGFFVKALHSVGAPLEPGQKDVAWPCSETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGV
APGTNWKSNPNAMRTIAAGNTVFTNVALTDDGDVWWEGLEGDPQHLIDWKGNDWYFRETETNAAHPNSRYCTPMSQCPIL
APEWDDPQGVPISGILFGGRRKTTVPLVTEARDWQHGVFIGATLGSEQTAAAEGKVGNVRRDPMAMLPFLGYNVGDYFQH
WINLGKHADESKLPKVFFVNWFRRGDDGRFLWPGFGENSRVLKWIVDRIEHKAGGATTPIGTVPAVEDLDLDGLDVDAAD
VAAALAVDADEWRQELPLIEEWLQFVGEKLPTGVKDEFDALKERLG
>Mature_605_residues
TSATIPGLDTAPTNHQGLLSWVEEVAELTQPDRVVFTDGSEEEFQRLCDQLVEAGTFIRLNPEKHKNSYLALSDPSDVAR
VESRTYICSAKEIDAGPTNNWMDPGEMRSIMKDLYRGCMRGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRTMTR
MGKAALEKMGDDGFFVKALHSVGAPLEPGQKDVAWPCSETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMAH
DEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWMRFGKDGRLYAVNPEFGFFGVA
PGTNWKSNPNAMRTIAAGNTVFTNVALTDDGDVWWEGLEGDPQHLIDWKGNDWYFRETETNAAHPNSRYCTPMSQCPILA
PEWDDPQGVPISGILFGGRRKTTVPLVTEARDWQHGVFIGATLGSEQTAAAEGKVGNVRRDPMAMLPFLGYNVGDYFQHW
INLGKHADESKLPKVFFVNWFRRGDDGRFLWPGFGENSRVLKWIVDRIEHKAGGATTPIGTVPAVEDLDLDGLDVDAADV
AAALAVDADEWRQELPLIEEWLQFVGEKLPTGVKDEFDALKERLG

Specific function: Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle [H]

COG id: COG1274

COG function: function code C; Phosphoenolpyruvate carboxykinase (GTP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [GTP] family [H]

Homologues:

Organism=Homo sapiens, GI187281517, Length=612, Percent_Identity=52.124183006536, Blast_Score=619, Evalue=1e-177,
Organism=Homo sapiens, GI66346721, Length=603, Percent_Identity=50.9121061359867, Blast_Score=586, Evalue=1e-167,
Organism=Homo sapiens, GI66346723, Length=354, Percent_Identity=54.2372881355932, Blast_Score=376, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71989645, Length=601, Percent_Identity=48.7520798668885, Blast_Score=578, Evalue=1e-165,
Organism=Caenorhabditis elegans, GI71989658, Length=601, Percent_Identity=48.7520798668885, Blast_Score=576, Evalue=1e-164,
Organism=Caenorhabditis elegans, GI71989653, Length=592, Percent_Identity=48.8175675675676, Blast_Score=570, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI17555492, Length=615, Percent_Identity=48.9430894308943, Blast_Score=565, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI32564411, Length=538, Percent_Identity=51.6728624535316, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI32564407, Length=527, Percent_Identity=51.4231499051233, Blast_Score=517, Evalue=1e-147,
Organism=Caenorhabditis elegans, GI71989649, Length=504, Percent_Identity=49.0079365079365, Blast_Score=490, Evalue=1e-139,
Organism=Caenorhabditis elegans, GI32564409, Length=373, Percent_Identity=49.8659517426273, Blast_Score=350, Evalue=1e-96,
Organism=Caenorhabditis elegans, GI17540956, Length=488, Percent_Identity=24.7950819672131, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24655085, Length=602, Percent_Identity=50.4983388704319, Blast_Score=602, Evalue=1e-172,
Organism=Drosophila melanogaster, GI24655082, Length=599, Percent_Identity=51.5859766277129, Blast_Score=594, Evalue=1e-170,
Organism=Drosophila melanogaster, GI161077226, Length=569, Percent_Identity=52.0210896309315, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24655088, Length=503, Percent_Identity=52.0874751491054, Blast_Score=530, Evalue=1e-150,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018091
- InterPro:   IPR013035
- InterPro:   IPR008209
- InterPro:   IPR008210 [H]

Pfam domain/function: PF00821 PEPCK [H]

EC number: =4.1.1.32 [H]

Molecular weight: Translated: 67254; Mature: 67122

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00505 PEPCK_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSATIPGLDTAPTNHQGLLSWVEEVAELTQPDRVVFTDGSEEEFQRLCDQLVEAGTFIR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCEEE
LNPEKHKNSYLALSDPSDVARVESRTYICSAKEIDAGPTNNWMDPGEMRSIMKDLYRGCM
ECCCCCCCCEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRTMTRMGKAALEKMGDDGFFVKAL
CCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHH
HSVGAPLEPGQKDVAWPCSETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWM
CCCCHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCHHHCCCCEEEE
RFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMRTIAAGNTVFTNVALTDDGDVWWEGLE
EECCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCEEEECCC
GDPQHLIDWKGNDWYFRETETNAAHPNSRYCTPMSQCPILAPEWDDPQGVPISGILFGGR
CCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCHHHCCEECCCCCCCCCCCEEEEEECCC
RKTTVPLVTEARDWQHGVFIGATLGSEQTAAAEGKVGNVRRDPMAMLPFLGYNVGDYFQH
CCCEEEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
WINLGKHADESKLPKVFFVNWFRRGDDGRFLWPGFGENSRVLKWIVDRIEHKAGGATTPI
HHHHCCCCCHHHCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
GTVPAVEDLDLDGLDVDAADVAAALAVDADEWRQELPLIEEWLQFVGEKLPTGVKDEFDA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHH
LKERLG
HHHHCC
>Mature Secondary Structure 
TSATIPGLDTAPTNHQGLLSWVEEVAELTQPDRVVFTDGSEEEFQRLCDQLVEAGTFIR
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCEEE
LNPEKHKNSYLALSDPSDVARVESRTYICSAKEIDAGPTNNWMDPGEMRSIMKDLYRGCM
ECCCCCCCCEEEECCCHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RGRTMYVVPFCMGPLGAEDPKLGVEITDSEYVVVSMRTMTRMGKAALEKMGDDGFFVKAL
CCCEEEEEEEECCCCCCCCCCCCEEECCCCEEEEEHHHHHHHHHHHHHHCCCCCHHHHHH
HSVGAPLEPGQKDVAWPCSETKYITHFPETREIWSYGSGYGGNALLGKKCYSLRIASAMA
HHCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
HDEGWLAEHMLILKLISPENKAYYFAAAFPSACGKTNLAMLQPTIPGWRAETLGDDIAWM
CCCCHHHHHHHHHHEECCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCHHHCCCCEEEE
RFGKDGRLYAVNPEFGFFGVAPGTNWKSNPNAMRTIAAGNTVFTNVALTDDGDVWWEGLE
EECCCCCEEEECCCCCEEECCCCCCCCCCCCCEEEEECCCEEEEEEEEECCCCEEEECCC
GDPQHLIDWKGNDWYFRETETNAAHPNSRYCTPMSQCPILAPEWDDPQGVPISGILFGGR
CCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCHHHCCEECCCCCCCCCCCEEEEEECCC
RKTTVPLVTEARDWQHGVFIGATLGSEQTAAAEGKVGNVRRDPMAMLPFLGYNVGDYFQH
CCCEEEEEECCCCCCCCEEEEEECCCCCHHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
WINLGKHADESKLPKVFFVNWFRRGDDGRFLWPGFGENSRVLKWIVDRIEHKAGGATTPI
HHHHCCCCCHHHCCEEEEEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHCCCCCCCC
GTVPAVEDLDLDGLDVDAADVAAALAVDADEWRQELPLIEEWLQFVGEKLPTGVKDEFDA
CCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHCCCCCHHHHHH
LKERLG
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA