Click here to switch to the map view.

The map label for this gene is ytpA [H]

Identifier: 148659947

GI number: 148659947

Start: 215448

End: 216287

Strand: Direct

Name: ytpA [H]

Synonym: MRA_0191

Alternate gene names: 148659947

Gene position: 215448-216287 (Clockwise)

Preceding gene: 148659942

Following gene: 148659948

Centisome position: 4.87

GC content: 66.55

Gene sequence:

>840_bases
ATGACTACCACCCGGACTGAACGGAATTTCGCGGGCATCGGCGATGTGCGCATCGTCTACGACGTCTGGACGCCGGACAC
CGCGCCGCAAGCGGTGGTCGTGCTGGCCCATGGTCTGGGCGAGCATGCCCGCCGCTACGACCATGTCGCGCAGCGGCTCG
GCGCGGCCGGCCTGGTCACCTATGCGCTTGACCACCGCGGGCATGGCCGCTCGGGTGGCAAACGGGTGCTAGTGAGAGAC
ATCTCCGAGTACACCGCTGACTTCGACACCCTCGTTGGGATCGCCACCCGGGAATATCCCGGGTGCAAGCGCATCGTGCT
CGGGCACAGCATGGGCGGCGGCATTGTGTTCGCTTACGGTGTCGAACGTCCAGACAACTACGACCTGATGGTGCTTTCGG
CGCCGGCGGTGGCGGCACAGGACCTGGTGAGCCCGGTAGTGGCGGTTGCCGCCAAGCTTCTGGGCGTCGTGGTGCCCGGC
CTGCCGGTGCAGGAACTGGATTTTACTGCCATCTCTCGCGACCCTGAGGTGGTCCAGGCTTACAACACCGACCCACTCGT
GCACCACGGACGGGTTCCGGCCGGGATTGGCCGCGCGCTGCTGCAGGTGGGCGAGACCATGCCGCGGCGAGCACCGGCAT
TGACCGCGCCGCTGCTAGTGCTGCACGGCACCGATGACCGGCTGATCCCCATCGAGGGCAGCCGTCGCCTGGTCGAATGT
GTGGGATCGGCCGACGTGCAGCTGAAGGAGTATCCCGGGCTGTACCACGAGGTGTTCAACGAGCCGGAGCGCAACCAGGT
GCTCGACGATGTGGTCGCCTGGCTCACCGAGCGGTTGTAG

Upstream 100 bases:

>100_bases
GGCGGGCTCGCGGCCAGAGTTTTCTGCGAGCACACTCACGTCAATGAGCCTACGCAGAGTCTCCGACACTCTCACCGGAG
CAGCCGTTACGCTCCCGGTA

Downstream 100 bases:

>100_bases
GCCGAGCCGACCTGTCGCAGCCCTCCACTAGTTTTGGCGCCATGACCAACGACAAGATGCTGGCCCGCATCGCAGCCCTG
CTGCGCCAGGCCGAAGGCAC

Product: putative lysophospholipase

Products: NA

Alternate protein names: Bacilysocin biosynthesis protein ytpA [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MTTTRTERNFAGIGDVRIVYDVWTPDTAPQAVVVLAHGLGEHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKRVLVRD
ISEYTADFDTLVGIATREYPGCKRIVLGHSMGGGIVFAYGVERPDNYDLMVLSAPAVAAQDLVSPVVAVAAKLLGVVVPG
LPVQELDFTAISRDPEVVQAYNTDPLVHHGRVPAGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVEC
VGSADVQLKEYPGLYHEVFNEPERNQVLDDVVAWLTERL

Sequences:

>Translated_279_residues
MTTTRTERNFAGIGDVRIVYDVWTPDTAPQAVVVLAHGLGEHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKRVLVRD
ISEYTADFDTLVGIATREYPGCKRIVLGHSMGGGIVFAYGVERPDNYDLMVLSAPAVAAQDLVSPVVAVAAKLLGVVVPG
LPVQELDFTAISRDPEVVQAYNTDPLVHHGRVPAGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVEC
VGSADVQLKEYPGLYHEVFNEPERNQVLDDVVAWLTERL
>Mature_278_residues
TTTRTERNFAGIGDVRIVYDVWTPDTAPQAVVVLAHGLGEHARRYDHVAQRLGAAGLVTYALDHRGHGRSGGKRVLVRDI
SEYTADFDTLVGIATREYPGCKRIVLGHSMGGGIVFAYGVERPDNYDLMVLSAPAVAAQDLVSPVVAVAAKLLGVVVPGL
PVQELDFTAISRDPEVVQAYNTDPLVHHGRVPAGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVECV
GSADVQLKEYPGLYHEVFNEPERNQVLDDVVAWLTERL

Specific function: Phospholipase involved in the biosynthesis of the antibiotic bacilysocin. It probably catalyzes the hydrolysis of the 2-sn-acyl moiety of phosphatidylglycerol to produce bacilysocin (lysophosphatidylglycerol). Is also able to catalyze the hydrolysis react

COG id: COG2267

COG function: function code I; Lysophospholipase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily [H]

Homologues:

Organism=Homo sapiens, GI51242953, Length=259, Percent_Identity=36.6795366795367, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI6005786, Length=259, Percent_Identity=36.6795366795367, Blast_Score=169, Evalue=3e-42,
Organism=Escherichia coli, GI1787678, Length=277, Percent_Identity=24.9097472924188, Blast_Score=72, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6322756, Length=305, Percent_Identity=25.9016393442623, Blast_Score=85, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022742 [H]

Pfam domain/function: PF12146 Hydrolase_4 [H]

EC number: NA

Molecular weight: Translated: 30262; Mature: 30131

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTTRTERNFAGIGDVRIVYDVWTPDTAPQAVVVLAHGLGEHARRYDHVAQRLGAAGLVT
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHH
YALDHRGHGRSGGKRVLVRDISEYTADFDTLVGIATREYPGCKRIVLGHSMGGGIVFAYG
HHHHCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEE
VERPDNYDLMVLSAPAVAAQDLVSPVVAVAAKLLGVVVPGLPVQELDFTAISRDPEVVQA
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECCCCHHEEE
YNTDPLVHHGRVPAGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVEC
CCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHH
VGSADVQLKEYPGLYHEVFNEPERNQVLDDVVAWLTERL
HCCCCCEEHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TTTRTERNFAGIGDVRIVYDVWTPDTAPQAVVVLAHGLGEHARRYDHVAQRLGAAGLVT
CCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHHH
YALDHRGHGRSGGKRVLVRDISEYTADFDTLVGIATREYPGCKRIVLGHSMGGGIVFAYG
HHHHCCCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEEE
VERPDNYDLMVLSAPAVAAQDLVSPVVAVAAKLLGVVVPGLPVQELDFTAISRDPEVVQA
CCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEECCCCHHEEE
YNTDPLVHHGRVPAGIGRALLQVGETMPRRAPALTAPLLVLHGTDDRLIPIEGSRRLVEC
CCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHH
VGSADVQLKEYPGLYHEVFNEPERNQVLDDVVAWLTERL
HCCCCCEEHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]