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The map label for this gene is fadE2 [H]

Identifier: 148659918

GI number: 148659918

Start: 183347

End: 184558

Strand: Reverse

Name: fadE2 [H]

Synonym: MRA_0162

Alternate gene names: 148659918

Gene position: 184558-183347 (Counterclockwise)

Preceding gene: 148659923

Following gene: 148659917

Centisome position: 4.18

GC content: 67.16

Gene sequence:

>1212_bases
ATGTCGGCCAAGGCCATCGACTACCGCACGCGGTTGTCCGACTTCATGACCGAGCACGTCTTCGGCGCTGAGGCCGATTA
CGACGACTATCGCCGCGCGGCCGGACCGGCCGACCACACCGCGCCGCCGATCATCGAGGAACTGAAAACCAAGGCCAAAG
ACCGCGGCCTGTGGAACCTATTCCTGTCGGCCGAGTCGGGATTGACCAACCTGGAGTACGCGCCGCTGGCCGAAATGACC
GGATGGAGCATGGAAATCGCGCCCGAGGCACTCAACTGCGCCGCACCGGACACCGGCAACATGGAGATCCTGCACATGTT
CGGCACCGAGCAGCAACGGGCGCAATGGCTGCGGCCGCTGCTCGACGGCAAGATCCGCAGCGCCTTCTCGATGACCGAGC
CGGCGGTGGCCAGCAGCGACGCCCGCAACATCGAAACCACCATCTCCCGGGACGGTGCCGACTACGTCATCAACGGCCGT
AAGTGGTGGACGTCGGGAGCGGCGGACCCGCGGTGCAAGATCCTCATCGTGATGGGCCGCACCAACCCGGACGCAGCCGC
CCACCAACAGCAGTCGATGGTCCTCGTCCCGATCGACACCCCCGGCGTGACGATCGTTCGCTCCACACCGGTGTTCGGCT
GGCAGGACCGGCACGGCCACTGCGAGATCGACTACCACAACGTCCGGGTCCCGGCCACCAACCTGCTCGGCGAAGAGGGC
AGCGGATTCGCCATAGCCCAGGCCCGGCTGGGGCCGGGCCGTATCCACCACTGCATGCGTGCGCTGGGCGCGGCCGAACG
CGCCTTGGCACTCATGGTGAATCGCGTCCGCAACCGGGTGGCGTTCGGCCGTCCGCTAGCCGAACAGGGCGTCGTGCAAC
AGGCGATTGCTCAGTCCCGCAACGAAATCGACCAGGCAAGGCTGCTGTGCGAAAAGGCGGCGTGGACAATCGACCAACAT
GGCAACAAAGAGGCGCGCCACCTGGTCGCCATGATCAAGGCGGTGGCCCCGCGGGTGGCCTGCGATGTCATCGACCGCGC
AATCCAGGTCCACGGGGCCGCCGGCGTCAGCGACGACACCCCGCTGGCCCGGTTGTACGGCTGGCACCGCGCCATGCGCA
TCTTCGACGGTCCCGACGAGGTGCACCTGCGCTCCATCGCCCGCGCCGAGCTGTCGAGGGAGAAGTCCACCTTCGCTGCG
GCGGTCACCTGA

Upstream 100 bases:

>100_bases
AACGACGGCGAGCAGACGCAAAACCCCCTTCTCGTCGGCGTGTCGAGGGACTTATGCGTCTGCTAGCGCCGGTAGATATC
GTGGCGGAATGGACTTCGCG

Downstream 100 bases:

>100_bases
GATGGCTGTCCGTGAACTGCCGGGCGCGTGGAACTTTCGTGACGTCGCCGACACCGCAACCGCATTGCGGCCGGGGCGGC
TGTTCCGGTCCAGCGAGCTG

Product: acyl-CoA dehydrogenase FadE2

Products: 2,3-dehydroacyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSAKAIDYRTRLSDFMTEHVFGAEADYDDYRRAAGPADHTAPPIIEELKTKAKDRGLWNLFLSAESGLTNLEYAPLAEMT
GWSMEIAPEALNCAAPDTGNMEILHMFGTEQQRAQWLRPLLDGKIRSAFSMTEPAVASSDARNIETTISRDGADYVINGR
KWWTSGAADPRCKILIVMGRTNPDAAAHQQQSMVLVPIDTPGVTIVRSTPVFGWQDRHGHCEIDYHNVRVPATNLLGEEG
SGFAIAQARLGPGRIHHCMRALGAAERALALMVNRVRNRVAFGRPLAEQGVVQQAIAQSRNEIDQARLLCEKAAWTIDQH
GNKEARHLVAMIKAVAPRVACDVIDRAIQVHGAAGVSDDTPLARLYGWHRAMRIFDGPDEVHLRSIARAELSREKSTFAA
AVT

Sequences:

>Translated_403_residues
MSAKAIDYRTRLSDFMTEHVFGAEADYDDYRRAAGPADHTAPPIIEELKTKAKDRGLWNLFLSAESGLTNLEYAPLAEMT
GWSMEIAPEALNCAAPDTGNMEILHMFGTEQQRAQWLRPLLDGKIRSAFSMTEPAVASSDARNIETTISRDGADYVINGR
KWWTSGAADPRCKILIVMGRTNPDAAAHQQQSMVLVPIDTPGVTIVRSTPVFGWQDRHGHCEIDYHNVRVPATNLLGEEG
SGFAIAQARLGPGRIHHCMRALGAAERALALMVNRVRNRVAFGRPLAEQGVVQQAIAQSRNEIDQARLLCEKAAWTIDQH
GNKEARHLVAMIKAVAPRVACDVIDRAIQVHGAAGVSDDTPLARLYGWHRAMRIFDGPDEVHLRSIARAELSREKSTFAA
AVT
>Mature_402_residues
SAKAIDYRTRLSDFMTEHVFGAEADYDDYRRAAGPADHTAPPIIEELKTKAKDRGLWNLFLSAESGLTNLEYAPLAEMTG
WSMEIAPEALNCAAPDTGNMEILHMFGTEQQRAQWLRPLLDGKIRSAFSMTEPAVASSDARNIETTISRDGADYVINGRK
WWTSGAADPRCKILIVMGRTNPDAAAHQQQSMVLVPIDTPGVTIVRSTPVFGWQDRHGHCEIDYHNVRVPATNLLGEEGS
GFAIAQARLGPGRIHHCMRALGAAERALALMVNRVRNRVAFGRPLAEQGVVQQAIAQSRNEIDQARLLCEKAAWTIDQHG
NKEARHLVAMIKAVAPRVACDVIDRAIQVHGAAGVSDDTPLARLYGWHRAMRIFDGPDEVHLRSIARAELSREKSTFAAA
VT

Specific function: Unknown

COG id: COG1960

COG function: function code I; Acyl-CoA dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI211971008, Length=390, Percent_Identity=55.3846153846154, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI48976061, Length=390, Percent_Identity=55.3846153846154, Blast_Score=423, Evalue=1e-118,
Organism=Homo sapiens, GI38505218, Length=404, Percent_Identity=51.2376237623762, Blast_Score=408, Evalue=1e-114,
Organism=Homo sapiens, GI4557233, Length=281, Percent_Identity=32.0284697508897, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI21361497, Length=352, Percent_Identity=27.8409090909091, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI7656849, Length=289, Percent_Identity=30.4498269896194, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI4557231, Length=300, Percent_Identity=27, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI187960098, Length=300, Percent_Identity=27, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4501859, Length=302, Percent_Identity=28.1456953642384, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI76496475, Length=361, Percent_Identity=27.9778393351801, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4557235, Length=361, Percent_Identity=27.9778393351801, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI4501857, Length=307, Percent_Identity=28.3387622149837, Blast_Score=102, Evalue=5e-22,
Organism=Homo sapiens, GI226958414, Length=283, Percent_Identity=25.7950530035336, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI226958412, Length=283, Percent_Identity=25.7950530035336, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI87081958, Length=311, Percent_Identity=30.2250803858521, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI1786223, Length=349, Percent_Identity=24.9283667621776, Blast_Score=91, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562446, Length=368, Percent_Identity=52.445652173913, Blast_Score=378, Evalue=1e-105,
Organism=Caenorhabditis elegans, GI71985184, Length=323, Percent_Identity=30.9597523219814, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17505929, Length=298, Percent_Identity=29.8657718120805, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17534899, Length=352, Percent_Identity=28.6931818181818, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17551932, Length=299, Percent_Identity=30.1003344481605, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17533517, Length=292, Percent_Identity=29.4520547945205, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17508101, Length=287, Percent_Identity=28.9198606271777, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17569725, Length=331, Percent_Identity=27.4924471299094, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17506239, Length=282, Percent_Identity=27.6595744680851, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17570075, Length=285, Percent_Identity=27.719298245614, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17538396, Length=357, Percent_Identity=26.890756302521, Blast_Score=101, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI71990804, Length=215, Percent_Identity=32.093023255814, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI71985192, Length=240, Percent_Identity=30.8333333333333, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI32563615, Length=186, Percent_Identity=26.3440860215054, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI71982178, Length=252, Percent_Identity=26.1904761904762, Blast_Score=73, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356377, Length=386, Percent_Identity=29.5336787564767, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24646207, Length=347, Percent_Identity=31.1239193083574, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24660351, Length=286, Percent_Identity=29.7202797202797, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24666513, Length=284, Percent_Identity=29.5774647887324, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI281363737, Length=324, Percent_Identity=27.7777777777778, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI21355753, Length=288, Percent_Identity=23.6111111111111, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920834, Length=296, Percent_Identity=25.3378378378378, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.3

Molecular weight: Translated: 44344; Mature: 44213

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKAIDYRTRLSDFMTEHVFGAEADYDDYRRAAGPADHTAPPIIEELKTKAKDRGLWNL
CCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEE
FLSAESGLTNLEYAPLAEMTGWSMEIAPEALNCAAPDTGNMEILHMFGTEQQRAQWLRPL
EEECCCCCCCCCCCCHHHHCCCCEEECCHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHH
LDGKIRSAFSMTEPAVASSDARNIETTISRDGADYVINGRKWWTSGAADPRCKILIVMGR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEECCCEEECCCCCCCCEEEEEEEEC
TNPDAAAHQQQSMVLVPIDTPGVTIVRSTPVFGWQDRHGHCEIDYHNVRVPATNLLGEEG
CCCCHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEECEEECCHHHHCCCCC
SGFAIAQARLGPGRIHHCMRALGAAERALALMVNRVRNRVAFGRPLAEQGVVQQAIAQSR
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NEIDQARLLCEKAAWTIDQHGNKEARHLVAMIKAVAPRVACDVIDRAIQVHGAAGVSDDT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLARLYGWHRAMRIFDGPDEVHLRSIARAELSREKSTFAAAVT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
SAKAIDYRTRLSDFMTEHVFGAEADYDDYRRAAGPADHTAPPIIEELKTKAKDRGLWNL
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEE
FLSAESGLTNLEYAPLAEMTGWSMEIAPEALNCAAPDTGNMEILHMFGTEQQRAQWLRPL
EEECCCCCCCCCCCCHHHHCCCCEEECCHHHCCCCCCCCCEEEEEECCCCHHHHHHHHHH
LDGKIRSAFSMTEPAVASSDARNIETTISRDGADYVINGRKWWTSGAADPRCKILIVMGR
HHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCEEECCCEEECCCCCCCCEEEEEEEEC
TNPDAAAHQQQSMVLVPIDTPGVTIVRSTPVFGWQDRHGHCEIDYHNVRVPATNLLGEEG
CCCCHHHHCCCCEEEEEECCCCEEEEECCCCCCCCCCCCEEEEEECEEECCHHHHCCCCC
SGFAIAQARLGPGRIHHCMRALGAAERALALMVNRVRNRVAFGRPLAEQGVVQQAIAQSR
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
NEIDQARLLCEKAAWTIDQHGNKEARHLVAMIKAVAPRVACDVIDRAIQVHGAAGVSDDT
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PLARLYGWHRAMRIFDGPDEVHLRSIARAELSREKSTFAAAVT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acceptor

Specific reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]